全文获取类型
收费全文 | 32454篇 |
免费 | 2021篇 |
国内免费 | 1745篇 |
专业分类
林业 | 5541篇 |
农学 | 2298篇 |
基础科学 | 619篇 |
4013篇 | |
综合类 | 12343篇 |
农作物 | 1957篇 |
水产渔业 | 2717篇 |
畜牧兽医 | 2846篇 |
园艺 | 1173篇 |
植物保护 | 2713篇 |
出版年
2024年 | 170篇 |
2023年 | 557篇 |
2022年 | 851篇 |
2021年 | 1050篇 |
2020年 | 1117篇 |
2019年 | 1266篇 |
2018年 | 906篇 |
2017年 | 1359篇 |
2016年 | 1542篇 |
2015年 | 1186篇 |
2014年 | 1563篇 |
2013年 | 2110篇 |
2012年 | 2445篇 |
2011年 | 2227篇 |
2010年 | 1861篇 |
2009年 | 1899篇 |
2008年 | 1777篇 |
2007年 | 1917篇 |
2006年 | 1596篇 |
2005年 | 1297篇 |
2004年 | 1077篇 |
2003年 | 880篇 |
2002年 | 695篇 |
2001年 | 643篇 |
2000年 | 547篇 |
1999年 | 509篇 |
1998年 | 432篇 |
1997年 | 356篇 |
1996年 | 313篇 |
1995年 | 361篇 |
1994年 | 271篇 |
1993年 | 265篇 |
1992年 | 251篇 |
1991年 | 236篇 |
1990年 | 203篇 |
1989年 | 149篇 |
1988年 | 110篇 |
1987年 | 83篇 |
1986年 | 40篇 |
1985年 | 19篇 |
1984年 | 11篇 |
1983年 | 8篇 |
1982年 | 12篇 |
1981年 | 12篇 |
1980年 | 9篇 |
1979年 | 6篇 |
1978年 | 8篇 |
1977年 | 4篇 |
1976年 | 4篇 |
1955年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
大叶千斤拔—云南热区优良牧草种质资源 总被引:7,自引:0,他引:7
大叶千斤拔广泛分布于云南热区,其生态适应范围广,抗逆性强,是云南省热区值得开发利用的豆科灌木类优良牧草种质资源,同时,也是热区重要的水土保持植物和绿肥资源。 相似文献
102.
新疆农田土壤有机质含量及组成特征 总被引:3,自引:0,他引:3
目前新疆农田土壤有机质含量在5.30~24.21g/kg之间,平均14.65g/kg,与第二次土壤普查相比,新疆农田土壤有机质含量呈上升趋势;胡敏素碳含量占到有机质碳量的53%~70%,平均61%,腐殖酸碳量只占39%。 相似文献
103.
V. Artegoitia A. Meikle L. Olazabal J. P. Damin M. L. Adrien D. A. Mattiauda J. Bermudez A. Torre M. Carriquiry 《Journal of animal physiology and animal nutrition》2013,97(5):919-932
The objective was to evaluate the effect of body condition score (BCS) at 30 days before calving (−30 days) induced by a differential nutritional management, parity and week of lactation (WOL) on milk yield and composition, and milk casein and fatty acid composition. Primiparous and multiparous Holstein cows with high BCS (PH, n = 13; MH, n = 9) and low BCS (PL, n = 9; ML = 8) under grazing conditions were sampled at WOL 2 and 8 (before and after peak of lactation). Milk yield was greater in multiparous than in primiparous cows and tended to decrease from WOL 2 to 8 only in ML cows. Milk protein, fat and casein yields were greater in multiparous than in primiparous cows and decreased from WOL 2 to 8. Milk casein concentration in milk protein was greater in MH cows than in ML, PH and PL cows at WOL 2. Milk κ‐casein was greater, and β‐casein was less in multiparous than in primiparous cows. As lactation progressed, proportion of casein fractions were not altered. Only κ‐casein fraction was affected by BCS at −30 days as PL showed a higher concentration than PH. The de novo (4:0–15:1) and mixed‐origin fatty acids (16:0–16:1) in milk fat increased, whereas preformed fatty acids (≥17:0) decreased from WOL 2 to 8. Saturated (SAT) fatty acids tended to be greater and monounsaturated fatty acids (MUFA) were less in multiparous than in primiparous cows. High‐BCS cows had greater concentrations of polyunsaturated (PUFA), conjugated linoleic acid (CLA) as well as n‐6 and n‐3 fatty acids in milk fat than low‐BCS cows. The results indicate that casein and fatty acid fractions in milk were affected by parity and may be modified by a differential nutritional management during the pre‐calving period (BCS at −30 days) in cows under grazing conditions. 相似文献
104.
105.
Callose and β‐1,3‐glucanase inhibit Phytophthora cinnamomi in a resistant avocado rootstock 下载免费PDF全文
N. van den Berg J. B. Christie T. A. S. Aveling J. Engelbrecht 《Plant pathology》2018,67(5):1150-1160
Phytophthora root rot (PRR) of avocado, caused by Phytophthora cinnamomi, is a significant threat to sustainable production wherever the crop is grown. Resistant rootstocks in combination with phosphite applications are the most effective options for managing this disease. Recently, the mechanisms underpinning PRR resistance have been investigated by the avocado community. Here, biochemical assays and confocal and scanning electron microscopy were used to investigate early defence responses in PRR resistant and ‐susceptible avocado rootstocks. Zoospore germination and subsequent hyphal growth for the pathogen were significantly inhibited on the surface of resistant avocado roots. When penetration occurred in the resistant R0.06 rootstock, callose was deposited in the epidermal cells, parenchyma and cortex of roots. In addition, β‐1,3‐glucanase was released early (6 h post‐inoculation, hpi) in response to the pathogen, followed by a significant increase in catalase by 24 hpi. In contrast, susceptible R0.12 roots responded only with the deposition of lignin and phenolic compounds incapable of impeding pathogen colonization. In this study, PRR resistance was attributed to a timely multilayered response to infection by P. cinnamomi. 相似文献
106.
改则高寒荒漠草地改良效果 总被引:3,自引:0,他引:3
对西藏阿里地区改则县高寒荒漠退化草地,采用了围栏、围栏+灌溉及围栏+灌溉+补播3种改良措施,以围栏外天然草地为对照,通过研究几个改良措施对草地植物高度、盖度、密度、地上生物量、重要值及物种多样性的影响,寻求适宜于该类退化草地恢复的最佳措施。结果表明,3种改良措施与对照相比均能明显改善退化草地群落中所有植物的生长状况;围栏+灌溉、围栏+灌溉+补播措施可以提高高寒荒漠退化草地的物种多样性;与其他改良措施相比,围栏+灌溉+补播措施可以显著提高高寒荒漠退化草地物种均匀度;综合考虑,围栏+灌溉措施更适宜于改则县高寒荒漠退化草地的改良恢复。 相似文献
107.
Melatonin reduces apoptotic cells,SOD2 and HSPB1 and improves the in vitro production and quality of bovine blastocysts 下载免费PDF全文
TC Marques EC da Silva Santos TO Diesel LO Leme CF Martins MAN Dode BG Alves FPH Costa EB de Oliveira ML Gambarini 《Reproduction in domestic animals》2018,53(1):226-236
Effects of adding different concentrations of melatonin (10?7, 10?9 and 10?11 M) to maturation (Experiment 1; Control, IVM + 10?7, IVM + 10?9, IVM + 10?11) and culture media (Experiment 2; Control, IVC + 10?7, IVC + 10?9, IVC + 10?11) were evaluated on in vitro bovine embryonic development. The optimal concentration of melatonin (10?9 M) from Experiments 1–2 was tested in both maturation and/or culture media of Experiment 3 (Control, IVM + 10?9, IVC + 10?9, IVM /IVC + 10?9). In Experiment 1, maturated oocytes from Control and IVM + 10?9 treatments showed increased glutathione content, mitochondrial membrane potential and percentage of Grade I blastocysts (40.6% and 43%, respectively). In Experiment 2, an increase in the percentage of Grade I blastocysts was detected in IVC + 10?7 (43.5%; 56.7%) and IVC + 10?9 (47.4%; 57.4%). Moreover, a lower number and percentage of apoptotic cells in blastocysts were observed in the IVC + 10?9 group compared to Control (3.8 ± 0.6; 3.6% versus 6.1 ± 0.6; 5.3%). In Experiment 3, the IVC + 10?9 treatment increased percentage of Grade I blastocysts with a lower number of apoptotic cells compared to IVM /IVC + 10?9 group (52.6%; 3.0 ± 0.5 versus 46.0%; 5.4 ± 1.0). The IVC + 10?9 treatment also had a higher mRNA expression of antioxidant gene (SOD 2) compared to the Control, as well as the heat shock protein (HSPB 1) compared to the IVM + 10?9. Reactive oxygen species production was greater in the IVM /IVC + 10?9 treatment group. In conclusion, the 10?9 M concentration of melatonin and the in vitro production phase in which it is used directly affected embryonic development and quality. 相似文献
108.
109.
爵床提取物对辣椒炭疽病菌的抑制作用及其苗期防效 总被引:1,自引:0,他引:1
爵床(J usticia procumbens L.)是一种传统的中药材.为探明爵床提取物对辣椒炭疽病菌的抑制作用,采用生长速率法和孢子萌发法分别测定了爵床提取物对辣椒炭疽病菌菌丝生长、孢子产生和孢子萌发的抑制作用,采用苗期盆栽试验观察爵床提取物对辣椒炭疽病的防治效果.结果表明,爵床甲醇提取物可抑制辣椒炭疽病菌菌丝生长、孢子产生和孢子萌发,其活性随着浓度的提高而增强,抑制中浓度分别为4.23、2.16和2.98 mg/mL.通过对爵床提取物处理后病菌孢子萌发形态显微观察,发现病菌孢子芽管长度受到明显抑制甚至不能萌发.当提取物浓度为16mg/mL时对苗期辣椒炭疽病的防效达73.08%. 相似文献
110.
镉对草莓幼苗根尖氧化系统和基因组DNA损伤的影响 总被引:4,自引:1,他引:4
采用随机扩增多态性DNA(RAPD)技术和生理生化方法检测镉胁迫对草莓幼苗根尖氧化系统和DNA多态性的影响。结果表明,用5、10和15 mg · L-1镉(CdCl2 · 2.5H2O)处理6 d后,草莓幼苗根伸长及根系中可溶性蛋白质含量均受到抑制,根尖活性氧爆发(超氧阴离子产生速率升高和过氧化氢含量增加),超氧化物歧化酶、过氧化物酶和过氧化氢酶活性下降,DNA增色效应减少。选用8条寡核苷酸引物(10 bp)对草莓幼苗根尖细胞中基因组DNA进行RAPD扩增,对照组图谱中可分辨出88条RAPD谱带,其分子量为150 ~ 3 500 bp,处理组与对照组RAPD图谱之间存在明显差异,且与镉浓度之间存在剂量效应关系。镉影响草莓幼苗根尖细胞中基因组模板的稳定性,活性氧爆发和DNA交联是根尖DNA损伤的主要原因,利用RAPD技术获得的DNA多态性变化可作为检测草莓根尖DNA损伤的指标。 相似文献