河北农业大学图书馆电子资源利用调查分析

通过读者问卷调查的方法,对河北农业大学图书馆电子资源利用情况进行了调查、分析,并根据调查结果提出了问题的解决方案及合理化发展建议。     

农户参与农房抵押贷款意愿的研究——基于山东、安徽228户农户的调查

通过对山东省潍坊市、安徽省安庆市的228户农户进行实地问卷调查,研究并分析了农户对农房抵押贷款的参与意愿及相关原因。研究结果表明:农户对于农房抵押贷款具有一定参与意愿,不同地区农户参与意愿有着较为明显的差异,被调查农户中潍坊市农户参与意愿高于安庆市农户;对农房生活保障功能的依赖是阻碍农户参与的主要原因;金融机构的正规性、自身的偿还能力以及政策细节与稳定性是农户在选择参与农房抵押贷款时所关注的主要方面;在政策期望方面,简化抵押贷款的手续步骤、加大政策宣传、降低贷款利息等得到较高关注。     

不同薏苡品种黑穗病发生情况调查

为筛选出适合宁化县推广种植的抗黑穗病品种，于2013年对当地种植的5个薏苡品种黑穗病发生情况进行调查，结果表明：龙薏一号黑穗病发病率最低，表现对黑穗病的抗性强，且产量较高，每667m2产量为257．43kg，适宜在薏苡黑穗病重发区推广种植。     

Infections Investigation of ALV and REV in Guangxi Local Poultry Breeds and Sequence Analysis of gp85 Gene of ALV Isolates

In order to investigate the infection status of avian leukosis virus (ALV) and avian reticuloendotheliosis virus (REV) in the major local breeds of Qinzhou,Guangxi,totally 953 samples of egg white,cloaca swab and serum of Ma duck,Shitou goose,Tiejiao-Ma chicken,turkey and pigeon were collected from the representing flocks and detected by the commercial ELISA kits.ALV was isolated for the ALV p27 positive samples by culturing on DF-1 cells,and gp85 gene was sequenced.The results showed that the detections of ALV were negative in the samples except those of Tiejiao-Ma chicken,while REV antibody was found positive in Ma duck,Tiejiao-Ma chicken and turkey.The nucleotide sequences of gp85 gene of two isolates shared 94.5% identity with each other,and shared 86.9% to 94.9% with reference strains.The amino acid sequences of gp85 gene of two isolates shared 91.5% identity with each other,and shared 84.0% to 91.6% with reference strains.There were many variable sites in the hyper variable region hr1 and hr2,and the vr2 and vr3 variable regions were relatively conservative.Phylogenetic tree analysis showed that the two isolates shared the highest homology with SCAU11-XG strain.     

河南省郏县红牛肠道寄生虫感染情况调查

为了解河南省郏县红牛肠道寄生虫感染情况，采用卢戈氏碘液染色法、饱和蔗糖溶液漂浮法和改良抗酸染色法对河南省郏县地区某红牛养殖场及散养红牛的218份新鲜粪便样本进行了检查，结果发现寄生虫总感染率为66.5%，共检出4种肠道寄生虫，其中球虫、圆线虫、鞭虫和绦虫感染率分别为61.5%、2.3%、6.9%和7.8%，混合感染率为11.5%，阳性粪便中虫卵或卵囊感染强度以轻度感染为主。结果表明，河南省郏县红牛肠道寄生虫感染较为普遍，应加强其肠道寄生虫病的防治。     

合福高铁沿线旅客旅游行为分析

在问卷调查的基础上,对合福高铁旅客的出游目的、旅游目的地偏好、旅游线路等旅游消费行为特征进行分析,结果表明:预订率最高的3趟班次依次为G28(09:52~13:31)、G322(09:12~13:36)、G1612(16:50~21:22);旅客出行目的以商旅为主;旅游目的地偏好依次为黄山、婺源、武夷山、三清山;旅客普遍使用旅游相关的APP。合福高铁沿线应从微旅游市场开发、移动智能营销平台建设、高铁配套设施、区域旅游合作等方面加强旅游联合营销。     

甘肃省兴隆山区域丛枝菌根真菌多样性初探

本研究采用Illumina HiSeq分子测序技术,探究了兴隆山东山丛枝菌根(Arbuscular mycorrhizal,AM)真菌多样性及其在不同海拔高度的分布特征。结果表明:1)自兴隆山东山土壤分离获得AM真菌共52种,隶属于4目8科13属,其中球囊霉属(Glomus)和原囊霉属(Archaeospora)为主要物种,分别为20种和9种,共占总物种的55.77%;2)各海拔梯度AM真菌种属分布结果表明,优势属球囊霉属(Glomus)和类球囊霉属(Paraglomus)随海拔变化呈现规律性分布,其余各属均匀分布于不同海拔梯度;球状巨孢囊霉(Gigaspora margarita)、蜜色无梗囊霉(Acaulospora mellea)、白色球囊霉(Glomus albidum)、球囊霉属的Glomus sp.1和Glomus sp.4,还有弯丝硬囊霉(Sclerocystis sinuosa)仅在部分海拔梯度出现,为不同海拔特有种;3)AM真菌丰富度分析表明,从低海拔到高海拔,ACE和Chao1指数总体上表现出“单峰”变化曲线,且部分海拔间差异性显著(P<0.05)。Spearman相关性分析发现,该地区AM真菌种属分布受海拔因子影响较小,两者间不具有显著相关性。综上,兴隆山东山AM真菌资源丰富,物种繁多,具有较大的应用潜力。     

Questionnaire on the process of recovering horses from general anesthesia and associated personnel injury in equine practice

ObjectiveTo collect data about the current practice of recovering horses from general anesthesia and recovery personnel safety.Study designOnline survey.MethodsAn online questionnaire, including questions on general demographic data, recovery drugs, modality and characteristics of equine recovery and morbidity and mortality, was designed and distributed via e-mail to equine practitioners worldwide.ResultsPractitioners from 22 countries completed 373 questionnaires; 53% of the participants were board-certified equine surgeons, and the remainder were board-certified anesthesiologists (18%), large animal residents (8%), general practitioners (7%), large animal interns (6%), anesthesia residents (4.5%) and veterinary technicians (1.6%). Respondents were employed by academia (58%) or private practice (42%). Of the respondents employed at a university, 93% had a board-certified anesthesiologist on staff compared with 7% of respondents employed at a private practice. Most of the respondents assist horses during recovery, with 23% assisting every recovery and 44% assisting recovery in the majority of cases. Reasons for choosing to assist horses during recovery were: orthopedic procedures (57%), neurological deficits (49%), bad health (47%), history of poor recovery (44%), foals (42%), draft breeds (30%), magnetic resonance imaging (17%) and computed tomography (16%). Unacceptable recoveries were reported by 77% of participants. Commonly reported complications during recovery with any method were: orthopedic injury (66%), myopathy (54%), skin abrasion (53%) and airway obstruction (37%). The incidences of unacceptable quality of recovery (p = 0.09) or personnel injury (p = 0.56) were not different between assisted and nonassisted recoveries; however, more equine fatalities were reported for assisted recoveries (p < 0.006). Practitioners in academia reported more unacceptable recoveries (p < 0.0007) and personnel injuries (p < 0.002) compared with those in private practice.ConclusionsThe method of recovery differs among hospitals. Recovery personnel injuries associated with assisting horses during recovery are an important and previously unreported finding.     

Identification and distribution of fungal pathogens associated with seedling blight of rice in the southern United States

Surveys were conducted in the five southern rice-producing states of Arkansas, Louisiana, Mississippi, Missouri and Texas in the United States during the 2018 and 2019 cropping seasons to determine the distribution and pathogenicity of fungal pathogens associated with seedling blight in rice. A total of 349 pathogenic fungal isolates were collected and identified as belonging to four genera: Rhizoctonia solani, Fusarium spp., Sclerotium rolfsii and Marasmius graminum based on morphological characteristics, molecular analysis and Koch's postulates. R. solani (252 out of 349 pathogenic isolates) was the most prevalent fungus isolated from diseased samples. Of the 252 pathogenic R. solani isolates, 245 were further classified as anastomosis group 11 (AG-11) and 7 as AG-4. Isolates of R. solani AG-4 and M. graminum were the most aggressive, with the highest stand loss (63% to 100%) and median disease rating (DR; 5.0), followed by isolates of R. solani AG-11 (stand loss = 4% to 100% and DR = 0.6 to 5.0), Fusarium spp. (stand loss = 26% to 48% and DR = 2.0 to 5.0) and S. rolfsii (stand loss = 33% to 48% and DR = 2.0 to 3.0) in causing seedling blight in rice. R. solani (62% to 83% of total pathogenic isolates) and Fusarium spp. (10% to 24% of total pathogenic isolates) were predominant in all the five states surveyed. S. rolfsii and M. graminum were present only in Louisiana and Texas. The results of this first systematic survey of rice seedling diseases in the southern United States will help develop effective fungicide seed treatment strategies for control of stand loss caused by seedling blight, one of the major factors limiting rice production.