The Fluorescent Characterization and Analysis of Two Brucella Attenuated Vaccine Infecting Mouse Macrophagocyte

The experiment was conducted to discuss the difference of binding time of green fluorescent protein B.melitensis M5 (GFP-M5) and B.abortus S19 (GFP-S19) infecting the mouse macrophagocyte (RAW264.7),lysosome,endoplasmic reticulum and golgi body in the initial stage and compare the binding rate of GFP-M5,GFP-S19 with organelle in different timeline,respectively,by confocal laser scanning microscope (CLSM) and flow cytometry.The result showed that GFP-M5 and GFP-S19 were successfully constructed.The intracellular survival ability of Brucella M5,Brucella S19,GFP-M5 and GFP-S19 were not obvisouly affected after infecting RAW264.7.GFP-M5 and GFP-S19 could enter the macrophagocyte in 30 mins,and in 2 h the Brucella could reach lysosome,endoplasmic reticulum and golgi body.In addition,the binding time for two attenuated vaccine did not show differences in 1,2,3 and 4 h.The content of GFP⁺ cell produced by RAW264.7 infected by GFP-M5 and GFP-S19 did not show significant differences (P>0.05).Therefore,the two strains did not have significant differences in the invasion ability in the initial stage of infecting host cell.     

上海松江中心城区道路绿化类型及景观分析

将松江中心城区道路绿化的配置形式归纳为14个类型,并对各类型的绿化景观效果进行了分析。     

经济绿肥覆盖对西双版纳胶园土壤性状的综合影响

论述了经济绿肥覆盖对提高土壤肥力、改善土壤物理性状、水土保持、提高抗病性、增加收入等方面的作用和重要意义。阐明了经济绿肥覆盖在西双版纳胶园的应用前景，它将实现经济效益和生态效益并重的胶园可持续发展。同时提出了在胶林行间特别是保护带陡坡地种植绿肥覆盖物的发展思路及相应措施，即完成胶园经济绿肥良种的筛选、经济效益和生态效益评价、及配套栽培技术试验示范。     

Reuolution and Research of Green Automobile

The green is as a symbol of no pollution,no public harm and environmental protection. The green automobile is the inevitable results that the automobile technique continuously developes. A new style vehicle--the green automobile is the urgent solicit of environmental protection is developed. The automobile industry can develop continuously. This article mainly discusses the importance of the green auto_mobile's research and development,the development status in quo and prospect of the green auto_mobile technique in the world,and states the relationship between the green automobile technique and environmental protection from green technical system theoretically clarify the study of green automobile,including its design and the system,the manufacture and its technology,the energy,the rebuild engineering and a series of subjects. How to evaluate the cycle and the evaluable mark of the green automobile,the prospect of green automobile are pointed out.     

Stability Ishikawa Iteration Procdeure for Strongly Pseudocontractive Operator and Strongly Accretive Operator Equations

By virtue of some analysis techniques,it is proved that certain ishikawa iteration procdeure with errors are stable with respect to strongly pseudocontractive operators in real Banach space. A related result deals with stable iteration procedures for solution of the nonlinear equation of strongly accretive operators. The results presented extend and improve the corresponding by Osilike,Zeng, Chidume, Deng, Liu,et al.     

EXISTENCE OF A BASIC OPTIMAL SOLUTION FOR LINEAR VECTOR EXTREME VALUE PROBBEM

In this paper, the concept of basic for linear vector extreme value problem is introduced in the partially ordered vector space. Some properties and results of the linear programming are generalized. And the existaence therom of basic optimal solution is proved.     

A NEW APPROACH OF OPTIMIZATION

In this paper, a necessary optimality condition of problems with equality constraints is investigated by the implicit function theorem in n-dimensional Euclidean space. A new approach solving these problems is obtained. The number of dimensions of corresponding system of equations is less than the classic Lagrangian multiplier method.