三江源区高寒草原土壤湿度变化特征及与气候因子的关系

利用青海省三江源区兴海县牧业气象站1999-2016年高寒草原土壤湿度、牧草生育期资料,分析了高寒草原土壤湿度的年、季变化特征以及牧草生长季不同生育期土壤湿度的变化特征及与气候因子的关系。结果表明:高寒草原0-10、10-20、20-30、30-40和40-50 cm土壤湿度均随年际延长呈增加趋势,春季除40-50 cm土层外,其他各层土壤湿度均随年际延长呈显著增加趋势(P<0.05)。高寒草原牧草生长季的土壤相对湿度随年际延长呈显著上升趋势(P<0.05),且与生长季降水量之间呈极显著正相关关系(P<0.01)。随着气候变化,牧草抽穗、开花、成熟和枯黄期的土壤相对湿度随年际延长呈显著增加趋势(P<0.05)。牧草抽穗期、枯黄和全生育期的土壤湿度与气温呈显著负相关关系(P<0.05)。高寒草原牧草生长季土壤湿度的增加有利于草地植被生长。     

Increasing selenium supply during the close-up dry period improves nutrient metabolism and attenuates inflammatory response after calving in dairy cows

The objective of this study was to investigate whether feeding selenium (Se)-replete cows a Se-yeast supplement in late pregnancy affects nutrient metabolism and inflammatory response during the periparturient period. Twenty cows were randomly assigned to two groups with 10 cows each. Cows in one group received Se-yeast at 0.3 mg Se/kg DM during the last 4 weeks before calving in addition to fed a TMR containing supplemented sodium selenite at 0.3 mg Se/kg DM (Se-yeast), while cows in another group were only fed a TMR containing supplemented sodium selenite at 0.3 mg Se/kg DM (Control). Blood samples were collected and analyzed for nonesterified fatty acids (NEFA), β-hydroxybutyrate (BHBA), glucose, insulin, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, serum amyloid A (SAA), haptoglobin (Hp), and albumin. In control cows, plasma NEFA, IL-1β, IL-6, SAA, and Hp levels increased after calving, but glucose, insulin, and albumin levels decreased after parturition. Se-yeast supplemental cows had lower postpartum concentrations of NEFA, TNF-α, IL-1β, IL-6, SAA, and Hp, and higher postpartum levels of glucose, insulin, and albumin compared with control cows. The results indicate that feeding Se-replete cows a Se-yeast supplement in late pregnancy improves nutrient metabolism and attenuates the inflammatory response after calving.     

天然生胶杂质含量测量结果不确定度评定

根据GB/T 8086—2008规定的测定方法,分析了测定过程中的随机效应与系统效应,计算这两种效应对测定过程导致的不确定度和,最终对本方法所产生的不确定度给予综合评定。     

棉铃发育过程中不同组织IAA含量变化特点

为了解IAA与棉纤维品质形成间的关系及棉铃各部位IAA积累转运特点,本研究分析了棉铃各部分在发育过程中IAA含量的变化特点。通过选择陆地棉标准系TM-1、长纤维种质‘母35’和短纤维种质‘208’3个纤维长度差异较大的陆地棉材料,利用酶联免疫法对浙江杭州、海南三亚两地田间试验的各材料不同发育阶段的棉仁、棉籽壳、棉纤维、棉铃壳进行了IAA含量的测定。结果表明,不同参试材料的棉仁IAA含量存在明显差异,棉籽壳、棉纤维与棉铃壳的IAA含量峰值出现时间晚于棉仁,棉铃各个组织IAA含量具有随棉铃组织从内向外逐渐降低的趋势。研究表明,IAA可能是棉纤维发育的正效应因子,且棉铃发育过程中IAA可能呈初期在棉仁富集、随发育进程沿棉铃组织由内向外运输的规律。     

氮添加对天山高寒草原土壤酶活性和酶化学计量特征的影响

为探究氮添加对高寒草原生态系统土壤酶活性的影响,于2018年在中国科学院巴音布鲁克草原生态系统研究站,选择4个氮添加水平(对照,N0,0 kg·hm^-2·a^-1;低氮,N1,10 kg·hm^-2·a^-1;中氮,N3,30 kg·hm^-2·a^-1;高氮,N9,90 kg·hm^-2·a^-1),开展土壤酶活性对氮添加响应的研究,分析土壤酶活性对氮添加的响应特点,土壤酶化学计量比以及土壤酶活性与土壤环境因子的关系。结果表明:与对照相比,氮添加在N3水平显著增加β-1,4葡萄糖苷酶(βG)、β-D-纤维二糖水解酶(CBH)和β-1,4木糖苷酶(βX)酶活性(P<0.05),N1和N3水平显著增加碱性磷酸酶(AKP)活性(P<0.05),N3水平显著降低多酚氧化酶(PPO)活性(P<0.05),氮添加对亮氨酸氨基肽酶(LAP)活性影响不显著,N3水平下显著增加N-乙酰-β-D氨基葡萄糖苷酶(NAG)活性(P<0.05)。相关分析表明,8种土壤酶活性均与土壤有机碳(SOC、NAG除外)和总磷(TP)显著相关,与土壤总氮(TN)不相关。研究区土壤酶活性C∶N∶P化学计量比为1∶1∶1.2,与全球生态系统的土壤酶活性C∶N∶P的比值1∶1∶1相偏离,表明该研究区土壤微生物生长受磷素限制。冗余分析(RDA)进一步揭示出土壤有机碳和土壤全磷含量是影响土壤酶活性的主要因子。     

不同胡椒粉中胡椒碱的含量比较

采用高效液相色谱法(HPLC)和紫外分光光度法(UV)测定黑胡椒、胡椒根、市售胡椒粉和用不同浸泡液浸泡脱皮的白胡椒粉中的胡椒碱含量。结果表明：黑胡椒粉、2种市售胡椒粉和白胡椒粉中胡椒碱含量不同；果＞根、白胡椒＞黑胡椒、盐水浸泡＞河水浸泡＞醋浸泡、西安胡椒粉＞海南胡椒粉。说明不同的胡椒粉胡椒碱的含量不同，这可以为胡椒的质量控制提供比较有用的参考。     

Lipid-rich bovine serum albumin improves the viability and hatching ability of porcine blastocysts produced in vitro

The effects of lipid-rich bovine serum albumin (LR-BSA) on the development of porcine blastocysts produced in vitro were examined. Addition of 0.5 to 5 mg/ml LR-BSA to porcine blastocyst medium (PBM) from Day 5 (Day 0 = in vitro fertilization) significantly increased the hatching rates of blastocysts on Day 7 and the total cell numbers in Day-7 blastocysts. When Day-5 blastocysts were cultured with PBM alone, PBM containing LR-BSA, recombinant human serum albumin or fatty acid-free BSA, addition of LR-BSA significantly enhanced hatching rates and the cell number in blastocysts that survived compared with other treatments. The diameter, ATP content and numbers of both inner cell mass and total cells in Day-6 and Day-7 blastocysts cultured with PBM containing LR-BSA were significantly higher than in blastocysts cultured with PBM alone, whereas LR-BSA had no effect on mitochondrial membrane potential. The mRNA levels of enzymes involved in fatty acid metabolism and β-oxidation (ACSL1, ACSL3, CPT1, CPT2 and KAT) in Day-7 blastocysts were significantly upregulated by the addition of LR-BSA. The results indicated that LR-BSA enhanced hatching ability and quality of porcine blastocysts produced in vitro, as determined by ATP content, blastocyst diameter and expression levels of the specific genes, suggesting that the stimulatory effects of LR-BSA arise from lipids bound to albumin.     

菜用睡莲营养成分及重金属含量测定与评价

对绿梗菜用睡莲(Nymphaea tetragona)及红梗菜用睡莲的15营养成分和4种重金属含量进行了测定与评价。结果表明,1与104~140种(品种)新鲜蔬菜已知数据比较,绿梗和红梗菜用睡莲均有较高含量的维生素C、钠及较低含量的干物质、蛋白质、钾、钙、镁、铜、锌、磷及硒;绿梗菜用睡莲有较高含量的粗纤维、锰及较低含量的铁,红梗菜用睡莲有较高含量的铁及较低含量的粗纤维。2菜用睡莲是一种含钠量较高的蔬菜。3绿梗菜用睡莲营养成分含量总体高于红梗菜用睡莲。4湖北产区菜用睡莲的铅、砷、镉及汞等重金属含量符合食品安全国家标准的规定。     

基于高光谱特征参数的冬小麦氮营养指数估算

为了实现快速高精度获取冬小麦氮营养指数的高光谱监测技术，利用美国SVC HR-1024I型野外光谱辐射仪对2017-2019年关中地区的冬小麦进行遥感监测，获取“三边”参数、任意两波段光谱指数和植被指数，通过相关性分析和逐步回归分析方法筛选冬小麦氮营养指数的敏感光谱参数，结合偏最小二乘回归(PLSR)、随机森林算法(RFR)、支持向量机回归(SVR)和梯度增强回归(GBDT)建立冬小麦氮营养指数模型，并对模型估算精度进行验证。结果表明，从拔节期到灌浆期，各时期的氮营养指数与任意两波段光谱指数均呈极显著相关，其中拔节期氮营养指数与任意两波段光谱指数相关性均高于其他时期，且基于一阶导数光谱的归一化光谱指数和比值光谱指数与氮营养指数的相关系数最大，为0.66。拔节期基于梯度增强回归的冬小麦氮营养指数预测模型的决定系数(r²)和均方根误差(RMSE)分别为0.96和0.05,模型验证的r²、RMSE和相对预测偏差(RPD)分别为0.95、0.12和2.12,模型预测精度最高。因此，拔节期基于梯度增强回归的冬小麦氮营养指数估算模型可用于冬小麦氮营养监测...     

Effects of Different Substrate Composition on Growth of Gesneriaceae Plants

The cultivation experiment was carried out to investigate the effects of different proportions of peat soil, perlite, vermiculite and yellow mud on growth of Gesneriaceae species(Chirita gueilinensis, Sinningia speciosa, Lysionotus pauciflorus, Hemiboea henryi, Aeschynanthus acuminatus, Saintpaulia ionantha). The growth traits of each plant growing in 7 different matrix materials were investigated.The plant height, crown width and chlorophyll content of each plant were measured. The results showed that the best substrate ratio was peat soil∶vermiculite=2∶1for C. gueilinensis, L. pauciflorus and H. henryi; peat soil∶perlite∶vermiculite = 2∶1∶1for S. ionantha; peat soil∶vermiculite∶yellow mud=2∶1∶1 for S. speciosa; peat soil∶perlite∶vermiculite∶yellow mud=2∶1∶1∶1 for A. acuminatus.