全文获取类型
收费全文 | 50134篇 |
免费 | 2704篇 |
国内免费 | 3347篇 |
专业分类
林业 | 13672篇 |
农学 | 3256篇 |
基础科学 | 2374篇 |
6367篇 | |
综合类 | 19029篇 |
农作物 | 2698篇 |
水产渔业 | 814篇 |
畜牧兽医 | 4545篇 |
园艺 | 1947篇 |
植物保护 | 1483篇 |
出版年
2024年 | 314篇 |
2023年 | 787篇 |
2022年 | 1332篇 |
2021年 | 1477篇 |
2020年 | 1448篇 |
2019年 | 1980篇 |
2018年 | 1228篇 |
2017年 | 1655篇 |
2016年 | 2251篇 |
2015年 | 2377篇 |
2014年 | 3160篇 |
2013年 | 3174篇 |
2012年 | 4179篇 |
2011年 | 3632篇 |
2010年 | 2965篇 |
2009年 | 2935篇 |
2008年 | 2634篇 |
2007年 | 2827篇 |
2006年 | 2635篇 |
2005年 | 2186篇 |
2004年 | 1638篇 |
2003年 | 1345篇 |
2002年 | 1094篇 |
2001年 | 1066篇 |
2000年 | 882篇 |
1999年 | 674篇 |
1998年 | 625篇 |
1997年 | 501篇 |
1996年 | 589篇 |
1995年 | 431篇 |
1994年 | 381篇 |
1993年 | 333篇 |
1992年 | 299篇 |
1991年 | 264篇 |
1990年 | 209篇 |
1989年 | 227篇 |
1988年 | 158篇 |
1987年 | 74篇 |
1986年 | 41篇 |
1985年 | 42篇 |
1984年 | 22篇 |
1983年 | 14篇 |
1982年 | 9篇 |
1981年 | 24篇 |
1980年 | 15篇 |
1979年 | 13篇 |
1978年 | 6篇 |
1976年 | 7篇 |
1975年 | 4篇 |
1955年 | 13篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
11.
12.
13.
The saddle panels, directly in contact with the horse's back, are likely an important element to optimize the fitting of the saddle, the comfort of the horse, and subsequently, the pain management in dorsalgic horses. The aim of this study was to better understand the effect of the saddle panels on the horse's back, by evaluating a prototype saddle (comfort panels: CP) compared to a standard saddle (STD). The horse's back movements were measured using inertial measurement units (IMUs) fixed at the levels of thoracic vertebrae T6, T12, T16 (under the saddle) and lumbar vertebrae L2 and L5. The centers of mass (COMs) of the horse and the rider and limb's protraction-retraction angles, pressure between saddle and horse's back, and force on the stirrups were measured using respectively 2D motion capture, pressure mat and force sensors in the stirrup leather. Three horses were trotted at the rising trot (sitting: left diagonal-rider seated; standing: right diagonal-rider standing) by the same rider. To compare saddles, linear mixed-effects regression models were used. The estimated means (SE) were calculated. During sitting phase, pressure in the cranial and middle areas of the saddle significantly increased for CP compared to STD (+0.9 (0.2) kPa and +1.0 (0.1) kPa, respectively) whereas caudal pressure decreased (−1.8 (0.4) kPa). Concurrently, the range of motion of angles T12-T16 and T16-L2 under the saddle significantly increased (+1.8 (0.2)° and +2.3 (0.3)°, respectively). The results showed that modifications of the panels' shape not only affect the pressure distribution but also the kinematics of the thoracic and lumbar regions of the equine back. 相似文献
14.
15.
Xiaomei Guo Lifen Ji Longfei Zhu Liling Song Shinan Liu Qiaolu Zang 《The Journal of Horticultural Science and Biotechnology》2018,93(2):142-149
It was reported that the activity of NiR (nitrite reductase) enzyme encoded by the NiR gene was correlated with the regeneration ability in rice. To testify the function of NiR gene in bamboo, seven bamboo species whose calli had different differentiation rate were chosen to analyse their NiR enzyme activity. The results showed that bamboo NiR enzymatic activity had a certain correlation with the regeneration capacity. A NiR gene named DhNiR from Dendrocalamus hamiltonii with high regeneration capacity was cloned. Sequence analysis revealed that the ORF of DhNiR was 1779bp encoding 592 amino acids. Overexpression of DhNiR in rice reduced the time of shoot differentiation and increased the transformation rate, suggesting that DhNiR might play an important role in the regeneration ability of bamboo, and can be applied in regeneration and gene transformation of bamboo and other plant species. 相似文献
16.
抗逆优质丰产棉花新品种的培育及应用是当前棉花生产节水提质增效的关键。‘衡棉1670’是河北省农林科学院旱作农业研究所以中早熟陆地棉品种资源材料‘衡棉210’为母本,以‘衡棉4号’为父本杂交,后代多年南繁北育,经旱棚盐池鉴定选择,在枯黄萎病混生重病地、不防治棉铃虫的高压胁迫下,经过多年连续定向选择育成。其突出表现为抗枯黄萎病、丰产优质、耐盐抗旱节水,适宜在河北省及黄河流域同类生态春播棉区推广种植,2019年8月通过河北省农作物品种审定委员会审定(审定编号为冀审棉20190013)。 相似文献
17.
18.
19.
为测定致羔羊脑炎粪肠球菌(Enterococcus faecalis)的生长曲线,寻求一种快速而准确的方法测定不同生长时期粪肠球菌数量,并客观评价其毒性强弱及其对小鼠脑组织的影响,试验采用平板菌落计数法和OD-Monitor振荡比浊法(Dλ值法)测定粪肠球菌的生长曲线,探究该菌在合适时间段内的吸光值(D600 nm)与平板菌落计数法测定的活菌数(CFU)的关系。用粪肠球菌感染小鼠,观察记录小鼠的死亡情况,最后采用Karber法计算粪肠球菌感染小鼠的半数致死量(LD50)。用LD50的剂量感染小鼠,及时采集死亡小鼠脑组织,未死亡的小鼠72 h后全部剖杀取脑组织,一部分做涂片染色,制作病理切片,观察病理变化;一部分进行培养,用于PCR方法进行细菌的回收鉴定。结果显示,用两种方法测定此株粪肠球菌的生长曲线基本一致,在2~8 h生长迅速,为对数生长期,8~14 h生长缓慢,为稳定期,14 h之后死亡数增加,进入衰亡期;对12 h粪肠球菌D600 nm与CFU的关系进行探讨,成功建立回归方程:y=20.769x-1.3422,R2=0.997;其感染小鼠的LD50为7.77×1011个活菌。以此剂量感染小鼠,脑组织涂片染色和培养染色,均能看到革兰氏阳性球菌;PCR结果显示,均出现了大小为112 bp的条带。对脑组织进行病理学观察发现该菌可导致脑组织充血、出血、形成微血栓,脑膜充血。通过生长曲线和其D600 nm与CFU关系的建立,可实时监测粪肠球菌数量,为后期更深入研究粪肠球菌穿越血脑屏障的机制奠定重要的理论基础。 相似文献
20.
l-proline (Pro) is a precursor of ornithine, which is converted into polyamines via ornithine decarboxylase (ODC). Polyamines plays a key role in the proliferation of intestinal epithelial cells. The study investigated the effect of Pro on polyamine metabolism and cell proliferation on porcine enterocytes in vivo and in vitro. Twenty-four Huanjiang mini-pigs were randomly assigned into 1 of 3 groups and fed a basal diet that contained 0.77% alanine (Ala, iso-nitrogenous control), 1% Pro or 1% Pro + 0.0167% α-difluoromethylornithine (DFMO) from d 15 to 70 of gestation. The fetal body weight and number of fetuses per litter were determined, and the small and large intestines were obtained on d 70 ± 1.78 of gestation. The in vitro study was performed in intestinal porcine epithelial (IPEC-J2) cells cultured in Dulbecco''s modified Eagle medium-high glucose (DMEM-H) containing 0 μmol/L Pro, 400 μmol/L Pro, or 400 μmol/L Pro + 10 mmol/L DFMO for 4 d. The results showed that maternal dietary supplementation with 1% Pro increased fetal weight; the protein and DNA concentrations of the fetal small intestine; and mRNA levels for potassium voltage-gated channel, shaker-related subfamily, member 1 (Kv1.1) in the fetal small and large intestines (P < 0.05). Supplementing Pro to either gilts or IPEC-J2 cells increased ODC protein abundances and polyamine concentrations in the fetal intestines and IPEC-J2 cells (P < 0.05). In comparison with the Pro group, the combined administration of Pro and DFMO reduced the expression of ODC protein and spermine concentration in the fetal intestine, as well as the concentrations of putrescine, spermidine and spermine in IPEC-J2 cells (P < 0.05). Meanwhile, the percentage of cells in the S-phase and the mRNA levels of proto-oncogenes c-fos and c-myc were increased in response to Pro supplementation, whereas depletion of cellular polyamines with DFMO increased tumor protein p53 (p53) mRNA levels (P < 0.05). Taken together, dietary supplementation with Pro improved fetal pig growth and intestinal epithelial cell proliferation via enhancing polyamine synthesis. 相似文献