燕麦叶片光合速率、气孔导度对光强和CO_2的响应与模拟

为指导燕麦生产,并了解燕麦在陆地生态系统水-碳循环过程中的作用,用LI-6400便携式光合测定仪,人工控制光强和CO2浓度,在锡林郭勒盟对燕麦叶片光合速率、气孔导度进行了研究,结果表明:燕麦叶片光合速率随光强的增强而增大,可以用米氏方程对其进行描述,在自然CO2浓度下,燕麦叶片的潜在最大光合速率为18.63μmol/(m2·s);在饱和光强下,光合速率随CO2浓度的增大而增大,也可以用米氏方程对其进行描述,CO2浓度→∞时,潜在最大光合速率达到110.28 μmol/(m2·s);气孔导度随光强的增强而增大,随CO2浓度的增大而减小;气孔限制值随光强的增强而增大,气孔限制值随CO2浓度的增大迅速增大,而[CO2]>200 μmol/mol时,气孔限制值基本保持恒定.     

基于冠层温度的玉米缺水诊断研究

冠层温度是判断植物水分状况的一项重要指标。以玉米良玉11为试验作物,在2014和2015年玉米抽雄期(7—8月)进行了4种不同梯度的土壤水分胁迫处理,研究了作物冠层温度、冠气温度比以及冠气温度差等指标与土壤含水量的关系,分析了冠气温度比与生理指标气孔导度的关系。研究结果显示:当土壤含水量低于0.16 cm3·cm~(-3)时,植株出现了明显的萎蔫现象,这时冠层温度、冠气温度比、冠气温度差也都分别达到了其最大值34℃,1.2℃和5℃;随着土壤含水量增大到0.20 cm3·cm~(-3),冠层温度等指标均达到其最小值,约为30℃,1.0℃和1℃,且变化比较稳定,这时植株生长良好,说明土壤供水充足,植株未受到水分胁迫。冠层温度等指标与气孔导度有显著的线性关系,气孔导度随着冠层温度等指标的增加而线性降低,表明气孔关闭会使得冠层温度显著提升。     

Effect of water deficit in different growth stages on stem sap flux of greenhouse grown pear-jujube tree

The effects of water deficit in different fruit growth stages on the variation of stem sap flux of 6-year old greenhouse-grown pear-jujube trees were investigated. Treatments included sufficient water supply during the whole fruit-growing period (T₁), mild water deficit during the flowering–fruit setting stage (T₂), moderate water deficit during the fruit rapid growth stage (T₃) and severe water deficit during the fruit maturing stage (T₄). Results showed that significant compensation effect on stem sap flux after re-watering was observed in T₂, but not in T₃ and T₄ stages. At the end of rapid growth stage, the diurnal variation of stomatal conductance generally had a similar trend as that of stem sap flux, but with a distinct midday depression from 12:00 to 14:00 p.m. In addition, a linear relationship between the relative available soil water content (RAWC) and the ratio of daily stem sap flux to that of sufficient water treatment was observed (R² = 0.4489).     

Physiological processes associated with salinity tolerance in an alfalfa half‐sib family

We previously reported an alfalfa half‐sib family, HS‐B, with improved salt tolerance, compared to parental plants, P‐B. In this study, we conducted additional experiments to address potential physiological mechanisms that may contribute to salt tolerance in HS‐B. Vegetatively propagated HS‐B and P‐B plants were treated with a nutrient solution (control) or a nutrient solution containing NaCl (EC = 12 dS/m). Shoots and roots were harvested at various time points after treatment for quantification of proline, soluble sugar, and H₂O₂ using spectrophotometers. Subcellular localization and quantification of Na in roots were conducted using a Na⁺‐specific dye under a confocal microscope. HS‐B produced 86 and 89% greater shoot and root dry biomass, respectively, compared to parental plants, P‐B, under salinity in the greenhouse. Under saline conditions the HS‐B shoots accumulated 115% and roots 55% more soluble sugars than P‐B counterparts. The non‐saline HS‐B shoots, however, showed 72% less soluble sugars than the non‐saline P‐B plants. Under saline conditions HS‐B accumulated 39% less proline in shoots, while roots accumulated 56% more proline, compared to their P‐B parents. HS‐B plants also showed a greater reduction of stomatal conductance under mild saline stress. HS‐B shoots and roots contained 3–4 times less reactive oxygen species (H₂O₂) after salt treatment compared to P‐B plants. Sodium localization and distribution analysis using Na⁺‐specific dye revealed HS‐B plants accumulated 88% and 48% less Na⁺ in stele and xylem vessels compared to P‐B. The study provides insights into the potential mechanisms that may contribute to salt tolerance in HS‐B: maintaining RWC by accumulating soluble sugars while reducing transpiration, maintaining healthy status by reducing oxidative stresses, and preventing salt toxicity by reducing accumulation of Na⁺ inside root cells and xylem.     

Electron Transport Through Photosystem II Is Not Limited By A Wide Range of Water Deficit Conditions In Field‐Grown Gossypium hirsutum

Despite exhaustive literature describing drought stress effects on photosynthesis in Gossypium hirsutum, the sensitivity of photosynthetic electron flow to water deficit is heavily debated. To address this, G. hirsutum plants were grown at a field site near Camilla, GA under contrasting irrigation regimes, and pre‐dawn water potential (Ψ_PD), stomatal conductance (g_s), net photosynthesis (P_N), actual quantum yield of photosystem II (Φ_PSII) and electron transport rate (ETR) were measured at multiple times during the 2012 growing season. Ψ_PD values ranged from ?0.3 to ?1.1 MPa. Stomatal conductance exhibited a strong (r² = 0.697), sigmoidal response to Ψ_PD, where g_s was ≤0.1 mol m^?2 s^?1 at Ψ_PD values ≤ ?0.86 MPa. Neither Φ_PSII (r² = 0.015) nor ETR (r² = 0.010) was affected by Ψ_PD, despite exceptionally low Ψ_PD values (?1.1 MPa) causing a 71.7 % decline in P_N relative to values predicted for well‐watered G. hirsutum leaves at Ψ_PD = ?0.3 MPa. Further, P_N was strongly influenced by g_s, whereas ETR and Φ_PSII were not. We conclude that photosynthetic electron flow through photosystem II is insensitive to water deficit in field‐grown G. hirsutum.     

美洲黑杨与大青杨杂种叶片气孔导度的红外热像测量方法研究

为了研究红外热像技术在测量叶片气孔导度的应用上的可行性,以美洲黑杨与大青杨杂种(Populus deltoids Bartr.×Populus ussuriensis Kom.)为材料,在正常灌溉和干旱胁迫的对比下,利用红外热像仪考察了叶片的气孔导度Gs的日变化,根据叶片能量平衡方程分析了气孔导度与温度之间的关系,并采用便携式光合测量系统验证了红外热像测量气孔导度的准确性。结果表明：在2种水分处理条件下,叶片的气孔导度值均呈双峰曲线,峰值出现在10:00和14:00,正常灌溉和干旱条件下的峰值分别为120 mmol/(m2?s)、70 mmol/(m2?s)和80 mmol/(m2?s)、40 mmol/(m2?s);气孔导度与温度的响应呈反比趋势;气孔导度的模型计算值与实际测量值之间的关系呈线性相关,正常灌溉和干旱状态下的相关系数分别为0.8678、0.8347;气孔导度相关指数IG和水分胁迫指数CWSI分别与测量得到的气孔导度呈正比、反比的趋势。总之,与传统的利用便携式光合仪测定气孔导度相比,利用红外热像技术测量更便捷、准确,且适用性更加广泛,本研究为今后利用红外热像测量气孔导度奠定了理论基础。     

Infrared Thermal Imaging as a Rapid Tool for Identifying Water‐Stress Tolerant Maize Genotypes of Different Phenology

The main task of this research was to evaluate canopy temperature and Crop Water Stress Index (CWSI) by assessing genotype variability of maize performance for different water regimes. To that end, three hundred tropical and subtropical maize hybrids with different phenology in terms of date of anthesis were evaluated. The influence of phenology on the change in canopy temperatures and CWSI was not equal during the three dates of measurement. At the end of vegetative growth (82 days after sowing, DAS) and at the blister stage (DAS 97), a high significant difference in temperatures and CWSI (P < 0.001) were obtained between the early‐ and late‐maturity genotypes. During anthesis (DAS 89), phenology had a significant effect (P < 0.01) only for the well‐watered genotypes, while under water‐stress conditions, no differences were found between early and late genotypes in terms of canopy temperature and CWSI. High significant differences (P < 0.001) in stomatal conductance (g_s) between early and late genotypes for different treatments were observed. A relationship (R² = 0.62) between g_s and canopy temperature was obtained. Under a water‐stress canopy, temperature was measured at anthesis, which was negatively correlated with grain yield of the early (r = ?0.55)‐ and late (r = ?0.46)‐maturity genotypes in the water‐stressed condition.     

Effects of Increasing Salinity Stress and Decreasing Water Availability on Ecophysiological Traits of Quinoa (Chenopodium quinoa Willd.) Grown in a Mediterranean‐Type Agroecosystem

Quinoa is a native Andean crop for domestic consumption and market sale, widely investigated due to its nutritional composition and gluten‐free seeds. Leaf water potential (Ψ_leaf) and its components and stomatal conductance (g_s) of quinoa, cultivar Titicaca, were investigated in Southern Italy, in field trials (2009 and 2010). This alternative crop was subjected to irrigation treatments, with the restitution of 100 %, 50 % and 25 % of the water necessary to replenish field capacity, with well water (100 W, 50 W, 25 W) and saline water (100 WS, 50 WS, 25 WS) with an electrical conductivity (EC_w) of 22 dS m^?1. As water and salt stress developed and Ψ_leaf decreased, the leaf osmotic potential (Ψ_π) declined (below ?2.05 MPa) to maintain turgor. Stomatal conductance decreased with the reduction in Ψ_leaf (with a steep drop at Ψ_leaf between ?0.8 and 1.2 MPa) and Ψ_π (with a steep drop at Ψ_π between ?1.2 and ?1.4 MPa). Salt and drought stress, in both years, did not affect markedly the relationship between water potential components, RWC and g_s. Leaf water potentials and g_s were inversely related to water limitation and soil salinity experimentally imposed, showing exponential (Ψ_leaf and turgor pressure, Ψ_p, vs. g_s) or linear (Ψ_leaf and Ψ_p vs. SWC) functions. At the end of the experiment, salt‐irrigated plants showed a severe drop in Ψ_leaf (below ?2 MPa), resulting in stomatal closure through interactive effects of soil water availability and salt excess to control the loss of turgor in leaves. The effects of salinity and drought resulted in strict dependencies between RWC and water potential components, showing that regulating cellular water deficit and volume is a powerful mechanism for conserving cellular hydration under stress, resulting in osmotic adjustment at turgor loss. The extent of osmotic adjustment associated with drought was not reflected in Ψ_π at full turgor. As soil was drying, the association between Ψ_leaf and SWC reflected the ability of quinoa to explore soil volume to continue extracting available water from the soil. However, leaf ABA content did not vary under concomitant salinity and drought stress conditions in 2009, while differing between 100 W and 100 WS in 2010. Quinoa showed good resistance to water and salt stress through stomatal responses and osmotic adjustments that played a role in the maintenance of a leaf turgor favourable to plant growth and preserved crop yield in cropping systems similar to those of Southern Italy.     

沙棘叶生理年龄与源库功能转化的关系

沙棘是喜光植物,属阳生叶。群体叶片,5月份生长速率慢,6月份加快,7月份最快,8月份开始减缓。一个枝条上新叶数占28.6%,成熟叶数76.7%,老叶数23.3%。自枝条顶端向下不同叶位叶之间存在着生理年龄上的差异,也存在着表观光合效率上的差异。叶序5～13为幼叶;15～31是成熟叶;33叶以为老叶。幼叶叶绿素含量较低,成熟叶含量最高,老叶又明显下降。气孔导度,也具有幼叶张开度小,成熟叶张开度最大,老叶张开度又变小。沙棘叶气孔CO2浓度也具有个体和生理年龄上的差异。     

Response of Vitis vinifera cv. ‘Tempranillo’ to partial rootzone drying in the field: Water relations, growth, yield and fruit and wine quality

This paper reports the effects of irrigation amount and partial rootzone drying (PRD) on water relations, growth, yield and wine quality of Vitis vinifera cv. ‘Tempranillo’ during two consecutive years in a commercial vineyard with a deep, light-clay soil located in Requena, Valencia, Spain. Partial rootzone drying applied at two amounts (100% and 50% of the estimated crop evapotranspiration), was compared to conventional drip irrigation, and also to rainfed vines. Results showed that the effects of irrigation amount on yield and wine quality were different between years. In 2003 with low yield values (around 6.3 t ha⁻¹) irrigation did neither affect grape production nor wine quality. However, in the following year, with much higher general yield (17 t ha⁻¹), the high irrigation dose increased yield by 30% compared to rainfed vines and it also increased must total soluble solids and wine alcohol content. In both seasons, PRD did not significantly affect physiological parameters, nor growth, yield or fruit and wine quality, when compared to the same amount of water applied by conventional drip irrigation. Overall these results suggest that, under our experimental conditions, it was the irrigation amount rather than the system of application what affected vine performance, indicating the difficulties of successfully employing the PRD type of irrigation with a drip system in heavy and deep soils.