微生物法测定婴幼儿配方乳粉中的生物素、叶酸和VB12

目的:采用微生物法测定婴幼儿配方乳粉中生物素、叶酸和VB12.方法:利用不同乳酸菌对特定维生素具有敏感性的特点,通过测定菌株的生长变化来反映样品中相应微生物的维生素含量,对市售的13种婴幼儿配方乳粉维生素进行测定.结果:微生物法对于生物素和VB12测定数据更稳定,重复性好,生物素相对标准偏差为0.056％～4.158％,VB12的相对标准偏差范围为0.112％～3.848％,而叶酸的检测数据之间波动稍大,相对标准偏差为1.236％～8.332％.结论:本法适合婴幼儿配方乳粉中生物素、叶酸和VB12这3种维生素的分析定量研究.     

Training methodologies differ with the attachment of humans to horses

Our investigation is the first to use attachment theory to explore four commonplace training methods (Conspecific, Behavioral, Eclectic, and Conventional) and owners' levels of attachment (anxiety and avoidance) to their horses. An international sample of horse riders and handlers (N = 538), aged 18-80 years, completed demographics and the Experiences in Close Relationships—Relationship Structures Questionnaire. Behavioral training participants scored significantly higher on the attachment-avoidance scores to their horses than the Eclectic or Conspecific training methods. The Behavioral and Eclectic training methods were associated with higher levels of education. Further research is warranted to elucidate the role that training methods play in the relationships between humans and horses.     

野生动物价值评估方法的评价

野生动物不同于一般的商品,它是一种缺乏市场价格的“公共商品”。因此对野生动物价值的评估方法也不同于一般的经济学方法。本文根据国内外野生动物价值评估的研究现状,主要介绍了两种实用的野生动物价值评估方法即旅行费用法（TCM）和条件价值评估法（CVM）,并对这两种方法的优点及局限性进行了评价。     

Analysis and experimental study on identification of inner force of steel tube under tension (compression) and bending using blind-hole method

为了解在役拉(压)弯钢管的内力情况,在盲孔法测量钢结构应力的基础上,提出了拉(压)弯钢管的内力识别方法.采用盲孔法对不同外径、不同荷载下的3组9根钢管试件进行了2种不同方式下的钻孔试验,研究了不同钻孔方式、不同外径、不同表面应力分布情况下的钻孔释放规律.试验结果表明:钻孔加工应变对测量精度影响较大,计算应力时应消除钻孔加工应变的影响;在应力超过f_y/2时需对释放系数进行塑性修正;钢管表面应力的测量精度随钢管直径的增大而提高.盲孔法用于拉(压)弯钢管的表面应力检测和杆件内力识别,无论理论上还是实际操作都是可行的,识别精度满足工程应用要求.     

副溶血弧菌检测技术的研究进展

副溶血弧菌引发食物中毒的规模呈明显的上升趋势,目前已超过沙门氏菌食物中毒,跃居首位。因此需要从多方面加强检测,以防止该菌对食品造成的大面积污染,保证人的身体健康。为此本文全面介绍了副溶血弧菌的各种检测方法,包括:传统方法,免疫学方法,分子生物学技术,传感器技术等,为副溶血弧菌的检测提供理论依据。     

本科酶工程课程教学实践与体会

酶工程是高校生物工程和生物技术专业重要的专业课。结合课程教学实践,总结了酶工程教学过程中的几点体会,以提高酶工程教学质量,达到学生综合能力的培养目的。     

新疆不同地区牛源大肠杆菌耐药性分析

This study was aimed to investigate the resistance of Escherichia coli isolates from cattle farms in Xinjiang to antibiotics. The resistances of Escherichia coli isolates from cattle farms in five regions to commonly used antibiotic were determined using microdilution broth assay. Escherichia coli isolates were highly resistant to ampicillin (up to 39.3%) in all regions, and existed multidrug-resistant, but zero resistance was the dominant. Escherichia coli isolates from E cattle farm in Cherqi town, Baicheng county, exhibited resistant to three kinds of quinolones (6.7%). The results suggested that the resistances of Escherichia coli isolates to commonly used antibiotics were not serious in Xinjiang.     

一例金毛犬胫骨斜骨折的髓内针逆向插入法联合钢丝内固定术治疗

通过对一例金毛犬胫骨斜骨折进行内固定术,详细介绍了髓内针逆向插法联合钢丝进行内固定术的手术要点,为同行进行犬胫骨斜骨折内固定术治疗提供参考。     

Non-iterative variance component estimation in QTL analysis

In variance component quantitative trait loci (QTL) analysis, a mixed model is used to detect the most likely chromosome position of a QTL. The putative QTL is included as a random effect and a method is needed to estimate the QTL variance. The standard estimation method used is an iterative method based on the restricted maximum likelihood (REML). In this paper, we present a novel non-iterative variance component estimation method. This method is based on Henderson's method 3, but relaxes the condition of unbiasedness. Two similar estimators were compared, which were developed from two different partitions of the sum of squares in Henderson's method 3. The approach was compared with REML on data from a European wild boar × domestic pig intercross. A meat quality trait was studied on chromosome 6 where a functional gene was known to be located. Both partitions resulted in estimated QTL variances close to the REML estimates. From the non-iterative estimates, we could also compute good approximations of the likelihood ratio curve on the studied chromosome.     

不同播种方式对岷山红三叶草产量的影响

通过在海拔2 450 m的岷县南部高寒阴湿地区进行岷山红三叶Trifolium pratense cv.Minshan干草丰产栽培试验,得到以下结论：条播为岷山红三叶进行干草生产的最佳播种方式,行距以20 cm最为理想,该行距下生长速度快,而且产草量最高。播种当年A组（行距20 cm）鲜草产量比对照组(撒播)高出4 987.5 kg/hm2,是对照组的1.27倍,差异极显著（P＜0.01）;A组鲜草产量比B组（行距40 cm）高101 kg/hm2,差异显著（P<0.05）。生长第2年,A组鲜草产量均高于对照组和B组,是对照组的1.16倍,差异极显著（P＜0.01）,是B组的1.02倍,差异显著（P＜0.05）。不同播种方式对岷山红三叶鲜干比无影响。