松嫩平原耕作黑土Cu、Pb、Zn的污染现状分析

黑土区是中国重要的商品粮生产基地之一,对于中国的粮食安全和国民经济的稳定起着举足轻重的作用。此文以松嫩耕作黑土土壤为研究对象,对呼兰、绥化、海伦、依安与嫩江5个调查区的土壤中Cu、Pb、Zn的污染现状进行研究,揭示耕作黑土的健康状况,为黑土生态系统的科学管理提供理论依据。采用内梅罗综合污染指数法及TOPSIS法进行环境质量综合评价。结果表明：土壤Cu、Pb、Zn的污染程度,由东南至西北未表现出明显的分布规律。呼兰、依安土壤Cu、Pb、Zn的污染程度低于绥化、海伦和嫩江;大部分样地土壤Cu、Pb、Zn污染程度具有随剖面深度的增加而增加的特点,仅有绥化土壤10~20 cm土层污染程度小于0~10 cm土层。Cu、Pb、Zn各元素之间没有呈现显著的相关性,表明3种元素来源是不同的。两种方法的评价结果基本一致,研究区域黑土环境质量良好。     

宁夏地区大籽蒿精油成分分析及其抑菌性研究

为探究宁夏大籽蒿(Artemisia sieversiana Ehrhart ex Willd.)精油成分、抑菌功能及安全性,采用水蒸气蒸馏法进行精油提取,通过气相色谱-质谱连用法(GC-MS)进行成分分析,采用滤纸片法测定其对3种菌的抑菌圈直径,测定最低抑菌浓度(MIC)、最低杀菌浓度(MBC),并通过cck-8法检测大籽蒿精油对于2种皮肤细胞的毒性作用。结果表明,宁夏大籽蒿精油的主要成分为母菊薁(29.61%),樟脑(4.80%)、桉叶油醇(4.32%)、异木香酸甲酯(4.02%);精油对铜绿假单胞菌和大肠杆菌的最低抑菌浓度为0.0781 μg/mL,最低杀菌浓度为0.3125 μg/mL;金黄色葡萄球菌MIC为0.0195 μg/mL,MBC为0.078125 μg/mL;当精油浓度小于100 μg/mL时对角质细胞(HaCaT)和皮肤成纤维细胞(NHSF)均无毒性。综上,宁夏地区大籽蒿精油具有较强的抑菌活性且安全性较高,以上结果为宁夏大籽蒿精油开发提供理论依据,以期为当地脱贫致富提供科技支撑。     

控制性隔沟交替灌溉条件下土壤蒸发的预测研究

棵间土壤蒸发是控制性交替灌溉条件下农田土壤耗水的重要组成部分,为了了解沈阳地区潮棕壤土条件下大豆田不同控制性交替灌溉制度下的土壤蒸发变化规律,对土壤蒸发进行预报,采用Micro-lysimeters(MLS)对不同灌溉处理下的土壤蒸发量进行了测定,并建立了以气象因子、土壤含水率和叶面积指数为自变量的土壤蒸发的预报模型.结果表明,在本试验的条件下,各处理的相对土壤蒸发率(E/ET0)与表层土壤含水率(θ)的关系均呈指数函数形式,决定系数在0.71以上.各处理的E/ET0与LAI之间亦呈很好的指数函数关系,决定系数均在0.78以上.     

THE WAYS FOR IMPROVING INDOOR ENVIRONMENT AND AIR-CONDITIONING SYSTEMS IN DANCLING HALLS

Since 1988, air temperature, humidity, air quality, noise level and intensity of illumination in lots of dancing halls in China have been investigated and tested. This paper introduces the present situation of indoor environment and air conditioning systems in dancing halls of China. The design indexes of cooling load for room and ontside air are recommended. The reasons for indoor environment deterioration have been found out. From the actual situation of China, the ways for imoroving indoor environment and approach of energy conservation in air conditioning systems are explored.     

玉米种子培养皿法发芽试验误差分析

培养皿法是玉米种子发芽试验常采用快捷方便的方法,但此方法的发芽率比较低、变异系数较大,不能反映种子本来固有的发芽水平。对此,笔者进行了培养皿法发芽试验的研究,结果表明,在培养皿内种胚向下是造成发芽率低和变异系数大的原因,若将种胚全部向上置放,就能减少发芽率的误差,体现出玉米种子的正常发芽水平。     

Effects of remifentanil on monophasic action potential and transmural dispersion of repolarization in rabbit myocardium

AIM: To study the effect of remifentanil on monophasic action potential and transmural dispersion of repolarization (TDR) in the 3-layer myocardium of isolated rabbit hearts. METHODS: Adult rabbits (n=18, 2.0 ~ 2.5 kg) were used to isolate the hearts for preparing Langendorff perfusion model. The hearts were randomly divided into 3 groups after perfusion with K-H solution for 15 min: the perfusion in control group (C group) continued for 60 min; the hearts in remifentanil group (R group) were perfused with 12 μg/L remifentanil K-H solution for 60 min; the hearts in remifentanil+aminophylline group (RA group) were given 60-min perfusion of 12 μg/L K-H remifentanil+30 mg/L aminophylline. The HR and 3 layers of myocardial monophasic action potential (MAP) in the left ventricular anterior wall were recorded at time points after balanced infusion for 15 min (T₀), and continued perfusion for 15 min (T₁), 30 min (T₂) and 60 min (T₃). The monophasic action potential duration of repolarization at 90% (MAPD₉₀) and the transmural dispersion of repolarization (TDR) were calculated. The early afterdepolarization, delay afterdepolarization and arrhythmia were also observed. RESULTS: In R group, slower HR and prolonger MAPD₉₀ and TDR at T₁~T₃ were observed as compared with those at T₀ (P<0.05). R group showed slower HR and longer MAPD₉₀ and TDR than C group and RA group (P<0.05). CONCLUSION: Remifentanil slows the HR, extends the MAPD₉₀ and increases the TDR, thus being prone to induce reentry. Aminophylline makes HR faster and MAPD₉₀ shorter, thereby reducing the TDR.     

银中杨人工林抚育采伐技术研究

采用标准地调查方法,对初植密度为2 m×2m的银中杨人工林的抚育采伐开始期和采伐强度进行调查研究,确定抚育采伐开始期为8年生时,采伐强度为50%。研究确定了银中杨人工林的经营密度表,以期为生产经营提供依据。     

EZH2-mediated regulation of NF-κB target gene expression in gastric cancer

AIM: To explore the mechanism by which over-expression of enhancer of zeste homolog 2 (EZH2) in a panel of gastric cancer cell lines is involved in tumorigenesis of gastric cancer. METHODS: Real-time PCR and Western blot were employed to examine the mRNA and protein levels of EZH2, respectively. MTS assay, cell migration and soft agar assay were performed to investigate the role of EZH2 in the regulation of stomach cancer behaviors. The effect of EZH2 on NF-κB target gene expression was determined by Luciferase reporter and real-time PCR. Co-immunoprecipitation was used to analyze the interaction of EZH2 and p65 in HEK293T cells. RESULTS: The expression levels of EZH2 were significantly increased in the gastric cancer cells compared with normal gastric epithelial cells. Pharmacological inhibition by DZNep or knockdown of EZH2 significantly compromised AGS and SNU-16 cell activity, cell migration and anchorage-independent cell growth. Moreover, siRNA knockdown of EZH2 impaired NF-κB downstream targets, such as IL-8, CXCL5 and CCL20. In addition, the interaction of EZH2 and p65 was detected. CONCLUSION: EZH2 mediates the growth of gastric cancer cells through the regulation of NF-κB downstream gene expression.     

南京市绿地规划发展方向初探--以南京市绿地系统规划（2013-2020）为例

对最新版本的《南京市绿地系统规划》(2013-2020)进行分析与研究，综合相关规划文件，对南京市近几年绿地规划、建设内容进行回顾与评价，总结南京市在绿地规划方面的可取与不足之处。对南京城市的历史沿革、山水格局、绿地现状、自身定位、发展趋势进行思考，结合国内政策、国外理念等因素统筹地对南京未来的绿地发展方向进行展望与研究，包括绿地宏观布局层面、中观联系层面、微观建设层面3方面。     

哈密设施桃红蜘蛛的危害与防治

新疆哈密地区特有的环境条件为红蜘蛛的发生创造了强有力的生态环境,经多年的栽种经验,归纳总结了红蜘蛛的发生规律、造成的危害以及综合防治方法,有效的防治红蜘蛛的发生。