由Cu(Ⅱ)促进的肌红蛋白选择性水解切割反应

采用聚丙烯酰胺凝胶电泳技术,研究了中性条件下[CuL(Cl)](C lO4)与马心肌红蛋白的选择性水解切割反应,L为2-[二(2-氨乙酸)氨基]乙醇.结果表明,[CuL(Cl)](ClO4)对马心肌红蛋白显示出较高的切割选择性和切割效率,且切割效率与浓度、介质条件、温度、温育时间有一定的相关性.     

Root parameters of forest trees as sensitive indicators of acidifying pollutants: a review of research of Japanese forest trees

The root parameters of forest trees can be indicators of a changing environment. We summarize the results of root studies with regard to the effects of acidifying pollutants, especially soil acidification and aluminum toxicity, on various root parameters of Japanese forest trees under experimentally controlled conditions. All root parameters such as biomass, morphology, nutritional status, and physiology can be regarded as indicators, because, under laboratory conditions, root responses occur prior to the responses in the aboveground parts. However, considering the conditions of forest sites, the nutritional status and physiological changes are better indicators of soil acidification and Al stress than the biomass and morphological response. The currently available data suggest that the most important indicator is the Ca/Al molar ratio in roots of Japanese tree species. In order to predict and detect the initial effects of soil acidification, we postulate that the specific root response to the Ca/Al molar ratio of tree roots should be considered as a parameter for use in long-term forest monitoring sites.     

Effects of chronic acid fog exposure with soil acidification or nitrogen loading on nutrient status in Abies firma seedlings

To clarify the nutrient status in momi fir (Abies firma Sieb. et Zucc.) seedlings under complex stress conditions of acid fog with soil acidification or nitrogen loading, we exposed seedling shoots to simulated acid fog (pH 3) and simultaneously loaded the soil with acid or excess nitrogen for 17 months. Proton and nitrogen loading reduced K concentration in soil, but these treatments had little effect on the nutrient status of fine roots in momi fir seedlings. Acid fog exposure resulted in reduced concentrations of Mg, Al, and Fe in current-year needles and Mg, Al, Cu, Fe, and Zn in 1-year-old needles. The complex effects of acid fog exposure with proton or nitrogen loading on nutrient status were relatively slight. However, elements such as Al and Cu notably reflect the effects of proton loading. These results show that the nutrient status of trace elements, rather than major elements, in needles of momi fir seedlings is sensitive to exposure to acid fog or proton loading.     

木荷凋落物分解及对土壤作用规律的研究

在福建省永春碧卿林场开展的木荷凋落物分解及土壤淋溶试验表明 :木荷人工林凋落物量和养分积累量下坡 >中坡 >上坡 ,养分积累量Ca >N >K >Mg >Fe >P >Zn >Cu ;凋落物分解的失重率Ws与时间t之间存在Ws =34 45 2Lnt -3 0 741(R =0 98)的对数关系 ;木荷凋落物可通过增加土壤有机质 ,提高土壤和下渗液的盐基量 ,提高土壤pH值 ,降低土壤水解性总酸度等途径来缓解土壤的酸化作用     

肉牛场生命周期估计及环境影响评价

试验采用生命周期估计(life cycle assessment,LCA)方法对江西高安某存栏1 800头的肉牛育肥场在整个育肥期间(7个月)的污染物排放量,以及污染物在全球变暖、环境酸化、富营养化、光化学臭氧合成方面对环境的影响进行了评估。系统边界包括化肥生产、农作物种植、饲料运输、肉牛生产和粪便处理。结果表明:温室气体排放量为(以CO2当量计)为11 908t,环境酸化气体排放量(以SO2当量计)为84.6t,富营养化气体排放量为(以PO43-当量计)为14.4t,光化学臭氧合成问题气体排放量为(以C2H4当量计)为1.68t。其中饲料运输主要影响全球变暖(占总排放量的49.8%)和光化学臭氧合成问题(占总排放量的54.1%),而粪便处理主要影响环境酸化(占总排放量的70.1%)和富营养化(占总排放量的76.4%),肉牛生产对环境的影响主要是全球变暖(占总排放量的18.1%)和光化学臭氧合成问题(占总排放量的30.9%)。因此,可考虑通过降低运输距离,改善饲料配方、粪便处理方式等降低污染物排放量。     

钙镁磷肥对元胡种植区酸化土壤改良效果的探究

为了探究钙镁磷肥对元胡种植区土壤酸化的阻控作用,通过连续两年的钙镁磷肥田间定位试验,以上元观镇当地元胡种植户施肥习惯为对照(CK),研究分析了在氮、钾肥用量相同的情况下,施用钙、镁、磷肥750 kg/hm~2、1 500 kg/hm~2和2 250 kg/hm~2,对土壤pH、交换性酸、阳离子交换量(CEC)、交换性盐基离子以及土壤基本肥力水平的影响。通过测算投入、产出效益,结果表明：施用钙镁磷肥能显著提升土壤pH水平,较(CK)处理提升0.27～0.31个单位,T100、T150两个处理能显著降低土壤交换性酸总量和交换性氢含量,对降低交换性铝有一定作用。施用钙镁磷肥较(CK)处理能显著提升阳离子交换量(CEC)以及交换性钙和交换性镁,是阻控土壤酸化的主要作用机制。但当施用量达到2 250 kg/hm~2时,种植元胡的新增纯收益会有所下降,伴随产生土壤中磷素过量的问题,会有造成磷素流失产生农业面源污染的风险。因此在汉中元胡种植区土壤酸化改良中推荐钙镁磷肥施用量750～1 500 kg/hm~2。     

生物炭及炭基肥影响农田土壤改良效应的研究进展

我国南方农田普遍存在土壤酸化加速、土壤肥力和质量不断下降、土壤微生物群落结构和功能失调等土壤退化现象。近年来,大量研究表明生物炭是一种应用前景广阔的土壤改良剂,而将其与肥料有效结合制成生物炭基肥,将在我国农田酸化土壤改良和作物增产等方面发挥重要作用。本文重点针对生物炭和生物炭基肥在农田生态系统中影响土壤改良的效应进行了综述,旨在为我国南方农田土壤改良和农业生产可持续发展提供参考。     

Effect of feeding acidified or fermented barley using Limosilactobacillus reuteri with or without supplemental phytase on diet nutrient digestibility in growing pigs

Fermentation of cereal grains may degrade myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate) (InsP₆) thereby increasing nutrient digestibility. Effects of chemical acidification or fermentation with Limosilactobacillus (L.) reuteri with or without phytase of high β-glucan hull-less barley grain on apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients and gross energy (GE), standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AAs), and standardized total tract digestibility (STTD) of P were assessed in growing pigs. Pigs were fed four mash barley-based diets balanced for water content: 1) unfermented barley (Control); 2) chemically acidified barley (ACD) with lactic acid and acidic acid (0.019 L/kg barley grain at a ratio of 4:1 [vol/vol]); 3) barley fermented with L. reuteri TMW 1.656 (Fermented without phytase); and 4) barley fermented with L. reuteri TMW 1.656 and phytase (Fermented with phytase; 500 FYT/kg barley grain). The acidification and fermentation treatments occurred for 24 h at 37 °C in a water bath. The four diets were fed to eight ileal-cannulated barrows (initial body weight [BW], 17.4 kg) for four 11-d periods in a double 4 × 4 Latin square. Barley grain InsP₆ content of Control, ACD, Fermented without phytase, or Fermented with phytase was 1.12%, 0.59%, 0.52% dry matter (DM), or not detectable, respectively. Diet ATTD of DM, CP, Ca, and GE, digestible energy (DE), predicted net energy (NE) value, and urinary excretion of P were greater (P < 0.05) for ACD than Control. Diet ATTD of DM, CP, Ca, GE, DE and predicted NE value, urinary excretion of P was greater (P < 0.05), and diet AID of Ca and ATTD and STTD of P tended to be greater (P < 0.10) for Fermented without phytase than Control. Diet ATTD of GE was lower (P < 0.05) and diet ATTD and STTD of P, AID and ATTD of Ca was greater (P < 0.05) for Fermented with phytase than Fermented without phytase. Acidification or fermentation with/without phytase did not affect diet SID of CP and AA. In conclusion, ACD or Fermented without phytase partially degraded InsP₆ in barley grain and increased diet ATTD of DM, CP, and GE, but not SID of CP and most AA in growing pigs. Fermentation with phytase entirely degraded InsP₆ in barley grain and maximized P and Ca digestibility, thereby reducing the need to provide inorganic dietary P to meet P requirements of growing pigs.     

炭基肥添加对蜜柚果园酸性土壤pH和交换性能的影响

为研究炭基肥添加对蜜柚果园酸性土壤pH和交换性能的影响,设不添加肥料空白(CK)、只添加尿素(N)、水稻秸秆炭基肥(rice straw biochar-based fertilizer,BR)和竹屑炭基肥(bamboo biochar-based fertilizer,BB)各4个用量梯度(2.5,5,10,20 g/kg)且配施尿素处理,开展60天室内培养试验。结果表明:随着培养进行,BR和BB处理土壤pH均先升高后降低,交换性酸和交换性盐基离子含量呈上升趋势。培养60天时,随着BR和BB施用量增加,对提升土壤pH、CEC及盐基饱和度,降低交换性酸、交换性铝等效果越明显,且BR效果优于BB。与N处理相比,BR和BB使土壤pH分别提升0.24~3.43和0.21~3.40个单位,交换性酸分别降低45.55%~97.25%和34.01%~96.70%,[JP]交换性铝分别降低46.95%~99.75%和38.99%~99.00%,CEC分别提升7.02%~170.74%和6.52%~134.90%,盐基饱和度分别增加29.40%~51.61%和32.08%~53.90%。施用炭基肥可以提高蜜柚果园酸性土壤pH,减少土壤交换性酸,大幅度降低交换性铝含量,同时提升土壤CEC和盐基饱和度。蜜柚果园土壤酸化治理炭基肥适宜用量为2.5~5.0 g/kg,水稻秸秆炭基肥效果优于竹屑炭基肥。研究结果为蜜柚果园酸化土壤治理提供科学依据。