香茅草中柠檬醛氢化还原橙花醇与香叶醇实验

本研究合成了固体催化剂Pt—Al2O3,以氢气和柠檬醛为主要原料,将其应用于柠檬醛的催化氢化还原反应中。通过实验主要考察了Pt负载量、催化剂用量、溶剂选择、反应温度等因素对催化反应的影响,并且确定了最佳反应条件:Pt的最佳负载量为1.6%,催化剂用量为5.5%,溶剂选择甲苯,温度选择为100℃。通过核磁共振,对所得目标产物进行了定性分析,确定目标产物为橙花醇与香叶醇。     

蒎烷的制备及其下游产品开发应用综述

介绍了由蒎烯催化加氢合成顺式蒎烷的工艺，以及蒎烷在合成芳樟醇、香叶醇和二氢月桂烯及其下游萜类产品的应用。     

川西獐牙菜SmGES基因的克隆、生物信息学分析与原核表达

研究藏药川西獐牙菜中环烯醚萜合成途径中的关键酶香叶醇合成酶的功能,并探讨其在香叶醇、龙胆苦苷和獐牙菜苦苷生物合成中的作用。以川西獐牙菜叶片为材料,提取RNA并反转为cDNA,通过川西獐牙菜转录组相关信息,用RT-PCR技术克隆得到了川西獐牙菜香叶醇合成酶基因,并命名为SmGES基因。将SmGES基因片段通过分子生物学的方法连接到原核表达载体pET-28a上并转化到Escherichia coli Rosetta(DE3)原核表达感受态中,进行相关的诱导表达。通过生物信息学进行相关分析得出,SmGES基因长1761 bp,编码586个氨基酸;SmGES蛋白等电点为5.35,相对分子质量为67.78 kDa。分析表明SmGES基因带有叶绿体转运肽,预测其是定位于叶绿体的亲水蛋白。文章还分析了该蛋白的细胞内定位、结构域、二级和三级结构。进化关系分析表明,SmGES基因与滇龙胆的GES基因具有较近的亲缘关系。诱导得到重组蛋白分子质量67.78 kDa的蛋白条带,与预期大小一致。研究结果为进一步阐明川西獐牙菜中环烯醚萜合成途径提供参考。     

Effect of manures and inorganic fertilizers on growth,yield, and essential oil of damask rose (Rosa damascena Mill.) and chemical properties of soil in western Himalayas

A field experiment was conducted to study different manures and inorganic fertilizers effect on damask rose during 2008–2012. The rose essential oil (EO) was obtained by hydro distillation in Clevenger type apparatus and the components in the rose oil were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). Application of 90:80:90 kg nitrogen: phosphorus: potassium (NPK) ha^?1 outshine all other treatments and recorded significantly higher flower and oil yield as compared to control. EO yield was also significantly correlated with flower yield plant^?1, number of flowers plant,^?1 and flower yield ha^?1. The higher percentage of citronellol+nerol was obtained by the application of 120:40:90 kg NPK ha^?1. Geraniol (26.2%) was maximum when the plants were fertilized with 90:40:90 kg NPK ha^?1. Citronellol+nerol/geraniol ratio was also high in fertilized plots. Furthermore, manure application resulted in a marked improvement in chemical properties (pH, percent organic carbon, available N, P, and K) of the soil.     

Weed control in rose-scented geranium (Pelargonium spp)

Abstract: Field investigations were carried out during 1999 and 2000 to identify effective chemical/ cultural methods of weed control in rose-scented geranium (Pelargonium spp). The treatments comprised pre-emergence applications of oxyfluorfen (0.15, 0.20 and 0.25 kg AI ha(-1)) and pendimethalin (0.50, 0.75 and 1.00kg AI ha(-1)), successive hand weeding, hoeing and mulching using spent of lemon grass (at 5 tonnes ha(-1)) 45 days after planting (DAP), three hand-weedings 30, 60 and 90 DAP, weed-free (frequent manual weeding) and weedy control. Broad-leaf weeds were more predominant than grass and sedge weeds, accounting for 85.8% weed density and 93.0% weed dry weight in 1999 and 77.2% weed density and 93.9% weed dry weight in 2000. Unrestricted weed growth significantly reduced geranium oil yield, by 61.6% and 70.6% in 1999 and 2000, respectively. Pre-emergence application of pendimethalin (0.75-1.00 kgAI ha(-1)) or oxyfluorfen (0.25 kg AI ha(-1)), successive hand-weeding, hoeing and mulching and three hand-weedings were highly effective in reducing weed density and dry weight and gave oil yield comparable to the weed-free check. Application of oxyfluorfen (0.15 or 0.20 kg AI ha(-1)) and pendimethalin (0.50 kg AI ha(-1)) were less effective in controlling the weed species in geranium. None of the herbicides impaired the quality of rose-scented geranium oil measured in terms of citronellol and geraniol content.