施肥处理对老芒麦种子质量和产量的影响

在河北省坝上地区鱼儿出牧场中国农业大学科技攻关实验站内，以种植2种和3年的老芒麦为试验材料，通过施肥处理（尿素130kg/hm^2＋磷酸二铵70kg/hm^2），研究测定种子产量和质量的变化。试验结果表明：施肥可以提高不同种植年限老芒麦种子产量，但对老芒麦种子质量的影响不一致，可明显提高种植第3年的种子质量，在种子活力测定中，加速老化测定、脱氢酶活性和酸性磷酸酯酶活性测定能较好地反映施肥对种子质量的影响，而活力指数、电导率值和浸种液含糖量测定结果变化不大，无法表现种子质量的变化。     

Selection potential of different prolificacy traits in the finnish landrace and large white populations

Heritabilities and genetic correlations for different prolificacy traits were estimated to assess possibilities of selection for high number of piglets weaned. Three litter-size traits: total number of piglets born (TNB), number of piglets born alive (NBA), number of piglets weaned (NW); four piglet survival traits: number of stillborn piglets (NSB), percent of stillborn piglets (NSB%), piglet mortality between birth and weaning (PM), percent of dead piglets during suckling (PM%); and three traits measuring time intervals: age at first farrowing (AFF), first farrowing interval (FFI), and gestation length (GL) were analysed. The Finnish national litter recording scheme provided data on the first parity litters of 11 329 Landrace and 8 362 Large White pigs born between 1986 and 2000. The heritabilitiy estimates were moderate for AFF and GL (0.24–0.37), and low for all the other traits (0.03–0.11). The genetic correlations between TNB and PM (0.68 in Landrace and 0.43 in Large White) and between NBA and PM (0.64 in Landrace and 0.31 in Large White) suggest that selection only for high TNB or NBA will lead to increased PM. The results showed further that GL will increase indirectly if the selection pressure is for low PM (r _g =?0.050 in Landrace and ?0.43 in Large White.     

河西荒漠灌区紫花苜蓿施肥效应及其基于数据包络分析法的经济效益研究

为科学准确地评判河西走廊苜蓿主产区紫花苜蓿饲草生产中施肥措施对其产品质量及生产收益的影响,本研究以“甘农3号”紫花苜蓿为材料,通过2016、2017年2年田间试验,以该区域紫花苜蓿饲草生产的平衡施肥推荐方案(N 103.5 kg·hm^-2、P₂O₅ 105 kg·hm^-2、K₂O 90 kg·hm^-2)为对照,探讨了不施肥及3种不完全施肥(缺氮偏施、缺磷偏施、缺钾偏施)处理下紫花苜蓿的生产性能,并采用数据包络分析法(DEA)对其经济效益进行分析。结果表明:与不施肥相比,施肥措施各处理均显著提高紫花苜蓿产量、蛋白总量,降低其酸性洗涤纤维和中性洗涤纤维,提高了相对饲用价值,从而改善了紫花苜蓿品质,并有效增加了经济效益;与氮、磷、钾平衡施肥相比,各偏肥处理的紫花苜蓿产量和品质均显著低于平衡施肥,尤以缺磷偏施的降幅最大,2016、2017年2年的产量和蛋白总量降幅分别达到25.9%、25.7%和33.4%、33.1%。因此,磷是河西荒漠灌区紫花苜蓿饲草生产的养分限制因子,氮、磷、钾对该地区紫花苜蓿生产性能影响顺序为:磷>氮>钾。运用数据包络分析法(DEA)分析出河西荒漠灌区紫花苜蓿的施肥效应为氮、磷、钾平衡施肥的经济效益最优,为DEA有效;不完全施肥的3个评价单元及不施肥评价单元为DEA无效,其中,不施肥经济效益最低,3个不完全施肥评价单元中的缺磷偏肥的紫花苜蓿经济效益比缺氮偏肥和缺钾偏肥更低;另外还以DEA模型推算出不同施肥措施下紫花苜蓿饲草生产经济效益改进的具体方案,其中,不施肥的紫花苜蓿饲草生产需调整的幅度最大,调整额度达10678.88 CNY·hm^-2,各施肥措施需调整的幅度排序为:不施肥>缺磷偏施>缺氮偏施>缺钾偏施。     

穗肥施用时期对水稻籽粒中胚乳蛋白积累的影响

以武香粳9号为材料研究了不同穗肥施氮时期对水稻籽粒蛋白质积累的影响.结果表明:穗肥的施用提高了籽粒发育过程中单位干重蛋白质含量,以在倒0.5叶施用氮素穗肥影响最大;随施肥时期的延迟,稻米粗蛋白和谷蛋白含量逐步上升,醇溶蛋白含量逐渐降低;倒2.5叶施氮减少了强、弱势粒中蛋白组分的分布和含量,倒0.5叶施氮与此相反;胚乳     

"黑土型"退化草地人工植被施肥试验研究

采用四因素二次通用回归旋转设计,以产草量为标准对“黑土型”退化草地上人工植被的施肥时间和氮磷钾的用量进行2年定位试验,建立牧草产量函数模型。结果表明,单因素增产效应为施肥当年:氮肥>磷肥>钾肥>施肥时间;第2年:氮肥>磷肥>施肥时间>钾肥。确定“黑土型”退化草地上人工植被的施肥组合方案为施肥时间在分蘖~7月上旬、N:75~130 kg/hm2、P2O5:50.0~112.5 kg/hm2、少施或不施钾肥,可取得较高的产量和经济效益。同时,为获得较高牧草产量,以每2年施肥1次为好。     

Malunion of an in utero open tibial fracture in an aborted equine fetus

An in utero tibial fracture was examined at necropsy in a seven-month-old male aborted Thoroughbred fetus. Grossly the healed fracture had a smooth cortical surface. Histologically there was no callus formation or excessive osteoclastic or osteoblastic activity.     

Microdroplet In Vitro Fertilization Can Reduce the Number of Spermatozoa Necessary for Fertilizing Oocytes

Successful in vitro fertilization (IVF) in mice has been achieved using spermatozoa at concentrations specifically optimized for the experimental conditions, such as species and source of spermatozoa. Although IVF in mice is mostly performed using about 80–500 µl drops, it is expected that the number of spermatozoa used for insemination can be reduced by decreasing the size of the IVF drops. The present study was undertaken to examine the extent to which the number of spermatozoa used for IVF could be reduced by using small droplets (1 µl). We devised the experimental parameters using frozen–thawed spermatozoa from C57BL/6 mice in anticipation of broader applications to other mouse facilities. We found that as few as 5 spermatozoa per droplet could fertilize oocytes (1 or 3 oocytes per droplet), although the fertilization rates were low (13–15%). Practical fertilization rates (> 40%) could be achieved with frozen-thawed C57BL/6J spermatozoa, which are sensitive to cryopreservation, when 20 sperm per droplet were used to inseminate 3 oocytes. Even with spermatozoa from a very poor quality suspension (10% motility), about 25% of oocytes were fertilized. Our calculations indicate that the number of inseminated spermatozoa per oocyte can be reduced to 1/96–1/240 by this method. In two separate embryo transfer experiments, 60% and 47%, respectively, of embryos developed to term. Our microdroplet IVF method may be particularly advantageous when only a limited number of motile spermatozoa are available because of inadequate freezing-thawing or genetic reasons.     

国内外致泻大肠埃希氏菌O157:H7的检测标准比较及建议

致泻大肠埃希氏菌O157:H7（STEC O157:H7）是大肠埃希氏菌中致病性最严重的一种食源性致病菌,主要存在于牛肉、牛奶、水果及其制品中,对身体健康造成很大危害,甚至引发死亡。食品中STEC O157:H7检测尤为重要。本文对国内外STEC O157:H7的检测标准进行比较,提出我国标准在样品前处理、快速筛选方法的应用等方面需要加强,以便为该菌快速准确检测提供帮助,实现与国际标准化体系建设接轨,满足实验室检测需要。     

近30年中国农田耕层土壤有机质含量变化

土壤有机质含量是影响土壤肥力水平的重要指标,也是开展耕地质量建设、科学施肥工作的基础。利用2005—2014年测土配方施肥项目数据与全国第二次土壤普查数据进行对比,分析了近30年来中国农田耕层土壤有机质的变化趋势。结果显示,30年来我国农田耕层土壤有机质含量呈整体上升趋势,目前,全国耕层土壤有机质平均含量为24.65 g kg~(-1),较全国第二次土壤普查时期提高4.85 g kg~(-1),提高24.49%。其中,30~40 g kg~(-1)等级比例增加3.64个百分点,20~30 g kg~(-1)等级比例增加5.68个百分点,10~20 g kg~(-1)等级比例增加5.36个百分点。需要引起注意的是,大于40 g kg~(-1)等级比例减少了1.38个百分点。