旅游管理本科专业人才培养模式的探讨

概述了我国高等院校旅游管理专业人才培养的研究现状,并在客观分析了当前我国旅游管理本科专业人才培养存在问题的基础上,提出了从人才培养目标定位、人才培养方案确立、人才培养途径选择、人才培养效果评估及反馈与调整等5个方面构建旅游管理本科专业人才培养模式的思路.     

工程图学三维模型库建立的研究

讨论了如何应用三维CATIA绘图软件开发工程图学模型库，以及三维模型库在教学中的作用和它对教学改革的影响。     

用B型超声波导引活体采集奶水牛的卵母细胞

本试验利用B型超声波导引活体采集42头奶水牛的卵母细胞,每头牛每周采卵2次,共采卵96次,卵母细胞回收率67%,可用卵母细胞数平均为3.1,成熟培养的囊胚率平均为24.7%.对水牛的卵巢观察结果表明活采对水牛的健康及生殖机能不产生影响.     

Sperm preparation through Sephadex™ filtration improves in vitro fertilization rate of buffalo oocytes

Routinely, swim‐up method is used to separate high‐quality sperm; however, long processing time and close cell‐to‐cell contact during the centrifugation step are inevitable elements of oxidative stress to sperm. The objective was to evaluate Sephadex^? and glass wool filtration to separate motile, intact and viable sperm for in vitro fertilization in buffalo. The cumulus–oocyte complexes (COC s) were collected from ovaries of slaughtered buffaloes by aspiration and matured for 24 hr in CO ₂ incubator at 38.5°C and 5% CO ₂. Matured COC s were rinsed twice in fertilization TALP and placed in the pre‐warmed fertilization medium without sperm. Cryopreserved buffalo semen was thawed at 37°C for 30 s and processed through Sephadex^?, glass wool filtration and swim‐up (control). Total and motile sperm recovery rates were assessed, resuspended in fertilization TALP and incubated for 15–20 min in CO ₂ incubator. Samples prepared by each method were divided into two aliquots: one aliquot was studied for sperm quality (progressive motility, membrane integrity, viability, liveability), while the other was subjected to co‐incubation with sets of 10–15 in vitro matured oocytes. Data on sperm quality were analysed by ANOVA , while in vitro fertilizing rates were compared by chi‐squared test using SPSS ‐20. Least significant difference (LSD ) test was used to compare treatment means. Glass wool filtration yielded higher total and motile sperm recovery rate, while Sephadex^? filtration improved (p  <  .05) sperm quality (progressive motility, membrane integrity, viability, liveability). Sperm preparation through Sephadex filtration yielded higher in vitro fertilization rate in terms of cleavage rate compared to glass wool filtration and swim‐up (control). In conclusion, cryopreserved Nili‐Ravi buffalo sperm selected through Sephadex filtration showed improved quality and yielded better fertilization rates (cleavage rate) of in vitro matured/fertilized oocytes. Sephadex filtration could be a promising technique for use in in vitro fertilization in buffalo.     

重庆市农民工回乡创业政策支持体系研究

通过对重庆市璧山县青杠街道、荣昌县昌州街道、武隆县火炉镇的回乡创业农民工的实地调研,分析重庆市农民工回乡创业的制度障碍,提出建构农民工回乡创业的政策支持体系。     

地下滴灌技术发展及应用现状综述

地下滴灌方式有诸多优点,已成为微灌技术应用的典型形式之一,近年来已引起国内业界人士的重视。本文对地下滴灌技术的发展及应用现状进行了综述,并讨论了研制出一种既具有良好压力流量关系又可抗负压堵塞能力的灌水器（ 滴头） ,以促进我国推广应用地下滴灌技术。     

我国的森林生物量研究

论述了我国森林生物量的研究内容及方法,对乔木层、林下植被、凋落物、粗木质残体、根系以及区域尺度的生物量研究进行了总结,概述了直接收获法、回归模型、平均换算因子法等常见的森林生物量估测方法.最后提出当前我国在研究重点分布、基础数据采集以及空间尺度转换等方面存在的一些问题,指出森林生物量研究在遥感监测等方面的发展趋势.     

含硫沉积物中有机氯农药的样品提取、净化与测定

利用GC-ECD研究了天津污灌区沉积物中16种有机氯农药的提取和测定方法。考察了加速溶剂提取的提取条件、样品净化方法和GC-ECD的测定条件。结果表明,在125℃用1∶1正己烷和二氯甲烷提取静态10min,2次循环,磺化,过florisil层析柱,铜粉脱硫后用GC-ECD测定可以得到很好的效果。主要7种物质的方法回收率在75%～91%之间,重现性较好。天津代表性沉积物中7种主要OCPs的实测浓度在0.380～2.093mg·kg-1范围内。     

VBA二次开发工具用于渐开线齿轮齿廓的绘制

采用一种嵌入AutoCAD内部基于对象的集成编程环境VBA来绘制齿轮齿廓,本文给出了具体实现方法和程序片段,探讨并验证了这种方法的可行性。     

青海省退牧还草工程与思考

青海省退牧还草工程实施9年来,已累计完成禁牧封育826.7×104hm2,占全省中度以上退化草地的45.6%。通过监测,退牧区草地植被盖度提高了15%～20%;牧草高度增加了3.5～5.5cm;草地牧草产量提高了26.45%。同时,草地生态环境得到了明显改善,草畜矛盾有所缓解,退牧还草工程的实施效果明显。建议,扩大该项工程在省内的实施范围,增加年度的实施规模,尽快完成全省的退牧还草任务,从而扩大工程的生态经济效益。