江苏沿岸定置张网主要渔获组成以及对经济鱼类幼体的损害分析

深水张网是吕四渔场传统的作业方式,渔获量占江苏省海洋捕捞总量的比重较大。根据2004年4月~2005年11月吕四渔场深水张网渔业监测资料,结合渔业统计数据,分析了该渔业渔获物种类组成及其季节变化,以及主要经济鱼类渔获物种类组成和幼鱼比例。结果表明,各季节深水张网优势渔获物种类组成具有明显的季节变化特征,春季主要以小黄鱼、黄鲫、焦氏舌鳎和葛氏长臂虾为主,夏季为小黄鱼、黄鲫、焦氏舌鳎和银鲳,秋季为小黄鱼、焦氏舌鳎、棘头梅童鱼、灰鲳和银鲳,冬季为小黄鱼、焦氏舌鳎、棘头梅童鱼和斑鰶;小黄鱼和焦氏舌鳎是深水张网渔业常年优势渔获物。主要经济鱼类小黄鱼以6月份平均网产最高,但主要以幼鱼为主,2004年和2005年的平均体重分别仅0.81 g/ind和1.04 g/ind,幼鱼渔获尾数分别占到99.1%和99.8%,累计损害幼鱼尾数分别达1.84×107ind和2.37×107ind;银鲳平均体重3.22 g/ind和3.8 g/ind,累计损害幼鱼尾数分别为4.84×105ind和4.41×105ind。建议对深水张网渔业加强管理,可对该渔业提前一个月休渔,提高网囊网目尺寸,并强化管理力度。     

基于核酸适配体的PCR法检测溶藻弧菌及其灭活菌

溶藻弧菌(Vibrio alginolyticus)分布广,数量多,发病率高,是水产养殖中常见的条件致病菌,而对溶藻弧菌进行快速准确的识别鉴定是其病害防治的前提和基础。核酸适配体,因为具有较高的亲和特异性,在微生物的识别鉴定方面展现出了巨大的优势。本文利用核酸适配体和适配体筛选产物,通过结合、洗涤、加热分离、PCR扩增以及电泳检测等步骤,对溶藻弧菌进行了检测鉴定。结果表明,适配体和筛选产物都能对溶藻弧菌及其灭活菌进行较好的识别鉴定,适配体筛选产物对溶藻弧菌的检测下限为10~3cfu/mL,而对其灭活菌的检测下限为10~2cfu/mL,适配体对溶藻弧菌及其灭活菌的检测下限都可达到10 cfu/mL。该方法对溶藻弧菌有较好的亲和特异性,并能较好地区分溶藻弧菌与哈维氏弧菌等水产常见病原菌,在水产病害的检测中显示了较好的应用前景。     

Odor Modification in Salmon Hydrolysates Using the Maillard Reaction

The aim of this work was to study the effect of adding sugar during proteolysis to promote the Maillard reaction and mask the initial fish odor and off-flavors generated. An experimental design, based on the Doehlert plan, was used to study the influence of hydrolysis conditions (time, temperature, sugar, and antioxidant addition) on the odor characteristics of hydrolysates, soluble protein levels, and amino acid content. Results showed that the lowest level of sugar (10 g of D-xylose added to 1 kg of by-products) was enough to develop a grilled odor in hydrolysates. In the hydrolysis conditions used—i.e., enzyme inactivation at 95°C for 30 min—hydrolysis temperature had no effect on grilled odor production but significantly affected the soluble protein fraction, as did hydrolysis time. Soluble protein content and essential amino acid content increased with the enzymatic reaction but were not modified by adding sugar. Hydrolysis conditions that promote Maillard reactions while keeping a nutritional balance have been identified.     

Degradation and effect of hydrogen peroxide in small‐scale recirculation aquaculture system biofilters

From an environmental point of view, hydrogen peroxide (HP) has beneficial attributes compared with other disinfectants in terms of its ready degradation and neutral by‐products. The rapid degradation of HP can, however, cause difficulties with regard to safe and efficient water treatment when applied in different systems. In this study, we investigated the degradation kinetics of HP in biofilters from water recirculating aquaculture systems (RAS). The potential effect of HP on the nitrification process in the biofilters was also examined. Biofilter elements from two different pilot‐scale RAS were exposed to various HP treatments in batch experiments, and the HP concentration was found to follow an exponential decay. The biofilter ammonia and nitrite oxidation processes showed quick recuperation after exposure to a single dose of HP up to 30 mg L^?1. An average HP concentration of 10–13 mg L^?1 maintained over 3 h had a moderate inhibitory effect on the biofilter elements from one of the RAS with relatively high organic loading, while the nitrification was severely inhibited in the pilot‐scale biofilters from the other RAS with a relatively low organic loading. A pilot‐scale RAS, equipped with two biofilter units, both a moving‐bed (Biomedia) and a fixed‐bed (BIO‐BLOK^®) biofilter, was subjected to an average HP concentration of ～12 mg L^?1 for 3 h. The ammonium‐ and nitrite‐degrading efficiencies of both the Biomedia and the BIO‐BLOK^® filters were drastically reduced. The filters had not reverted to pre‐HP exposure efficiency after 24 h, suggesting a possible long‐term impact on the biofilters.     

Upscaling as ecological information transfer: a simple framework with application to Arctic ecosystem carbon exchange

Transferring ecological information across scale often involves spatial aggregation, which alters information content and may bias estimates if the scaling process is nonlinear. Here, a potential solution, the preservation of the information content of fine-scale measurements, is highlighted using modeled net ecosystem exchange (NEE) of an Arctic tundra landscape as an example. The variance of aggregated normalized difference vegetation index (NDVI), measured from an airborne platform, decreased linearly with log(scale), resulting in a linear relationship between log(scale) and the scale-wise modeled NEE estimate. Preserving three units of information, the mean, variance and skewness of fine-scale NDVI observations, resulted in upscaled NEE estimates that deviated less than 4% from the fine-scale estimate. Preserving only the mean and variance resulted in nearly 23% NEE bias, and preserving only the mean resulted in larger error and a change in sign from CO₂ sink to source. Compressing NDVI maps by 70–75% using wavelet thresholding with the Haar and Coiflet basis functions resulted in 13% NEE bias across the study domain. Applying unique scale-dependent transfer functions between NDVI and leaf area index (LAI) decreased, but did not remove, bias in modeled flux in a smaller expanse using handheld NDVI observations. Quantifying the parameters of statistical distributions to preserve ecological information reduces bias when upscaling and makes possible spatial data assimilation to further reduce errors in estimates of ecological processes across scale.     

梨果实在贮藏过程中果胶分解酶活性的变化

以苹果梨和新高梨为试材,测定了梨贮藏过程中果胶分解酶的活性变化.结果表明:2品种果实在软化过程中多聚半乳糖醛酸酶(PG)活性均增加且在贮藏后期新高梨的PG活性极显著(p<0.01)高于苹果梨;贮藏性强的苹果梨的β-半乳糖苷酶(β-Gal)活性很低且在贮藏中减少,但贮藏性相对较弱的新高梨的β-Gal活性极显著(p<0.01)高于苹果梨且在可溶性果胶增加的时期达活性高峰;苹果梨的果胶甲基酯酶(PME)活性在贮藏后期有所增加,但新高梨减少.可见,在贮藏过程中PG和β-Gal酶均是梨果实软化的重要酶,但β-Gal的作用比PG酶更重要;PME的活性变化因品种而异,与耐贮性关系不大.     

双流道泵内非定常固液两相流的数值分析

采用Mixture多相流模型、标准的k-ε湍流模型与SIMPLEC算法,应用FLUENT软件对双流道泵内固液两相湍流进行了非定常数值模拟.研究结果表明:不同时刻,泵流道内的压力分布明显不同,呈现周期性变化规律;在叶轮流道内,固相体积浓度分布极不均匀,颗粒主要集中于叶轮出口处的工作面和后盖板上,而在蜗壳流道内,固相颗粒多集中于离叶轮半径较大的蜗壳壁面上.     

基于FLUENT的单-双涡室离心泵径向力分析

以某电厂3715L型脱硫泵为模型,应用商业软件FLUENT,采用标准k-ε湍流模型和SIMPLEC算法,对单-双涡室离心泵内部流场进行模拟,分析了这两种泵静压力和速度场的分布规律,并对径向力进行了计算分析.通过对比分析发现,单涡室离心泵在非设计工况点时隔舌两侧区域出现较大压差,作用于叶轮产生径向力,小流量时叶轮出口出现不对称的高速流体;双涡室结构能够有效地改善非设计工况点时压水室能量的转化,小流量时叶轮出口的高速液体呈对称分布,从而降低了压水室压差,起到了平衡径向力的作用;通过计算发现,偏离工况点时双涡室结构设计能有效地减小径向力.数值模拟的结果与现有理论的基本吻合,实际运行情况稳定,可以为更好地认识和设计双涡室离心泵提供依据.     

Application of the rpoS gene for the detection of Vibrio anguillarum in flounder and prawn by polymerase chain reaction

Vibrio anguillarum , an opportunistic fish pathogen, is the main species responsible for vibriosis, a disease that affects feral and farmed fish and shellfish, and causes considerable economic losses in marine aquaculture. In this study, we used polymerase chain reaction (PCR) to detect V. anguillarum . PCR specificity was evaluated by amplifying the rpoS gene, a general stress regulator, in six strains of V. anguillarum and 36 other bacterial species. PCR amplified a species-specific fragment (689 bp) from V. anguillarum . Furthermore, the PCR assay was sensitive enough to detect rpoS expression from 3 pg of genomic DNA , or from six colony-forming units (CFU) mL⁻¹ of cultured V. anguillarum . However, the assay was less sensitive when genomic DNA from the infected flounder and prawn was used (limit of detection, 50 ng and 10 ng g⁻¹ tissue, respectively). These data demonstrate that PCR amplification of the rpoS gene is a sensitive and species-specific method to detect V. anguillarum in practical situations.