不同品种烟草镉吸收的动力学研究

通过水培试验研究了不同品种烟草幼苗镉吸收的动力学特征及温度、代谢抑制剂对烟草镉吸收的影响。结果表明,烟草对镉的吸收Michaelis-Menten动力学方程I=Imax?C/(Km+C) 描述,不同品种间吸收的最大速率(Imax)为云烟85>G80、贵烟11号>Xanthi、CF965和RG17,Km为Xanthi>云烟85> G80>贵烟11号、RG17和CF965, ?（Imax/Km,根系对矿质元素的吸收能力）为贵烟11号?云烟85＞CF965＞G80＞RG17＞Xanthi。低温使各品种烟草镉的吸收量明显降低,代谢抑制使G80,Xanthi和CF965镉的吸收量降低50%以上,使RG17和云烟85镉的吸收量降低24%～44%,使贵烟11号镉的吸收量在营养液镉浓度为0.02mmol?L-1时降低55%,营养液镉浓度为0.22 mmol?L-1时降低34%。表明G80,Xanthi和CF965对镉的吸收以主动吸收过程为主,RG17和云烟85对镉的吸收以被动吸收过程为主,而贵烟11号镉的吸收在低镉浓度时以主动吸收过程为主,在较高镉浓度时以被动吸收过程为主。     

分子标记及其在云南稻种核心种质中的应用

本文总结了分子生物学发展的各类分子标记及其在核心种质中的应用。     

Classification of cassava into ‘bitter’ and ‘cool’ in Malawi: From farmers' perception to characterisation by molecular markers

Cassava roots, a major food in Africa, contain cyanogenic glucosides that may cause toxic effects. Malawian women farmers considered fields of seemingly similar cassava plants to be mixes of both ‘cool’ and ‘bitter’ cultivars. They regard roots from ‘cool’ cultivars as non-toxic. Roots of ‘bitter’ were considered to require extensive traditional processing done by women to be safe for consumption. But curiously, these women farmers preferred ‘bitter’ cultivars since toxicity confers protection against theft, which was a serious threat to the food security of their families. We studied how well these farmers comprehend the effects of genetic variations in cassava when dealing with cyanogenesis in this complex system. Using molecular markers we show that most plants farmers identified as belonging to a particular named cultivar had a genotype typical of that cultivar. Farmers' ethno-classification into ‘cool’ and ‘bitter’ cultivars corresponded to a genetic sub-division of the typical genotypes of the most common cultivars, with four-fold higher cyanogenic glucoside levels in the bitter cultivars. Examining morphology, farmers distinguished genotypes better than did the investigators when using a standard botanical key. Undoubtedly, these women farmers grasp sufficiently the genetic diversity of cassava with regard to cyanogenesis to simultaneously benefit from it and avoid its dangers. Consequently, acyanogenic cassava – the breeding of which is an announced good of some cassava genetic improvement programmes – is not a priority to these farmers. Advances in molecular genetics can help improve food supply in Africa by rapid micropropagation, marker assisted breeding and introduction of transgenic varieties, but can also help to elucidate tropical small-scale farmers' needs and skills. This revised version was published online in August 2006 with corrections to the Cover Date.     

Chloroplast DNA variation in cultivated and wild Prunus avium L: a comparative study

Chloroplast DNA variation in 96 Prunus avium L. cultivars was assessed and compared with the results of a previous study of cpDNA diversity in 23 wild populations of the species. The polymerase chain reaction‐restriction fragment length polymorphism method was used in these studies. Approximately 9% of the chloroplast genome was analyzed, using five universal primer pairs and three restriction enzymes. Ten polymorphic fragments were common to both the wild and sweet cherry; eight polymorphic fragments were found only in the wild cherry. In the cultivars, all mutations were small (5‐30 bp) indels. In the wild populations, a point mutation was also detected in addition to indels. The mutational combinations revealed three haplotypes in the cultivars, which are the main haplotypes in the wild cherry populations. Chloroplast DNA diversity in wild cherry is higher (16 haplotypes) than in sweet cherry cultivars (three haplotypes). The probable wild origin of the sweet cherry cultivars in the maternal line, on the basis of haplotypic similarity, was discussed.     

Methods for Rapidly Measuring the Lodging Resistance of Wheat Cultivars

Lodging resistance ratings for winter wheat cultivars are frequently based upon observations of lodging. This is an unreliable method because of the frequent occurrence of years without significant lodging events. It also does not distinguish between stem and root lodging resistance. This paper describes the development and testing of instrumentation and procedures for two field‐based methods to rapidly assess stem and root lodging resistance. Both methods used a specifically designed instrument for measuring the resistance of shoots against rotational displacement. Stem lodging resistance was assessed when the soil was dry and strong, whereas resistance to root lodging was assessed after the soil had been weakened by irrigation. Tests were carried out on 14 winter wheat cultivars grown at two sites in the UK during 2002. Both methods were able to detect statistically significant differences between the cultivars for their resistance to stem and root lodging. A comparison of these results with observations of lodging in the field showed that the methods accounted for about 60 and 50 % of the stem and root lodging respectively.     

江淮稻区不同穗型粳稻品种主要农艺和品质特性的比较分析

依据着粒密度，对江淮稻区大面积生产应用的和新近育成的36个粳稻品种(系)进行聚类分析。结果表明，供试品种可分成半密穗型(Ⅰ)、偏密穗型(Ⅱ)和密穗型（Ⅲ）三类。随着水稻品种由散穗型向半密穗型、偏密穗型和密穗型的演变，产量性状、品质性状、形态性状和部分生理、生化性状发生了明显的变化，产量、每穗总粒数和实粒数显著增加，而穗数/m²、千粒重和结实率呈下降趋势；株高增加，上部三张叶片变长、变宽，生物日产量增高；穗部一、二次枝梗数增多，着粒密度变大；抽穗期和成熟期叶片、茎秆的含氮量和叶片叶绿素含量增高；糙米率和整精米率下降、垩白粒率降低、胶稠度变硬、蛋白质含量呈上升的趋势。     

降水、灌溉和品种对玉米产量和水分利用效率的影响

8年田间试验结果表明,玉米产量明显受降水和灌溉的影响。玉米苗期灌溉最为关键,是保证高产的基础。在只灌溉苗水而没有其他灌溉条件下,夏玉米产量与7、8月份降水密切相关,而与生长季节的降水总量关系不明显。除了极干旱年份(季节降雨量小于200 mm)外,随着灌溉量增加,玉米水分利用效率(WUE)降低。玉米最优灌溉制度为:干旱年份,除了苗期的1次灌溉外,在拔节和扬花期分别实施2次灌溉;常年和湿润年份,除了出苗水外,在抽穗到扬花期的8月初实施1次灌溉。3年的田间试验结果表明,当地种植的不同玉米品种之间产量最大相差20%,水分利用效率差异在12%~19%。因此,将适宜的品种与优化的灌溉制度相结合,是提高华北平原夏玉米产量和水分利用效率的有效途径。     

不同青花菜品种形态和品质性状比较

筛选外观优异、品质优良的青花菜品种,以期为青花菜品质改良提供材料基础.笔者以国内常见的19个青花菜品种为材料,对其花球质量、花球直径、花球茎粗、花球形状、蕾粒大小、花球色泽、叶绿素、含水量、可溶性蛋白质、可溶性总糖及维生素C等形态和品质指标进行测定与分析.结果 表明,不同青花菜品种形态和品质之间存在一定差异,多数青花菜...     

短季棉主要农艺性状的遗传分析

选用5个早熟不早衰的短季棉品种和5 个早衰的短季棉品种进行部分双列杂交。通过对亲本、F1 和F2 代分别于2001 年和 2002 年两年田间试验研究。结果表明:子棉产量、皮棉产量和衣分3个性状以显性效应为主,其次为加性效应,同时还存在极显著的加性上位性与环境的互作效应,单铃重和成铃数以显性效应为主;与早熟有关的诸性状,生育期、始花期、铃期和果枝始节4个性状以加性效应为主,其次为显性效应,霜前花率以显性效应×环境互作效应为主,同时存在显著的加性上位效应,落叶株率以加性与加性互作上位性为主,落叶指数以加性效应为主;与纤维品质有关的诸性状,2.5%跨长、比强度、伸长率3个性状以加性效应为主,其次为显性效应,同时还存在着加性、上位性与环境的互作效应;同时还研究了产量、早熟性和纤维品质各性状之遗传和表型相关关系。