压砂西瓜间套马铃薯油葵栽培试验

针对宁夏压砂西瓜产业发展的需要,探索压砂西瓜高效栽培模式技术(间套)。结果表明:压砂西瓜套种油葵共生期长,前期油葵与西瓜争光和肥水,西瓜坐瓜和大小受限制,影响西瓜产量;压砂西瓜套种马铃薯共生期长,马铃薯与西瓜争光、争肥水不明显,西瓜坐瓜和大小不受限制,西瓜产量不受影响,每667 m2套种马铃薯生产比对照多收入231.2元。     

关于皆伐的思考

本文从景观层次上对皆伐的应用进行了分析 ,得出皆伐是森林主伐的一种行之有效的方式。在森林主伐中 ,应根据立地条件和景观格局 ,恰当考虑皆伐的应用。     

Research on Indoor CO2 Density in Chongqing Commercial Buildings

The density of indoor CO 2 is an important value for newly_built commercial buildings. It affects comercial buildings in two aspects, one is the indoor air_quality, the other is the consumption of energy. The aim of design is to keep good indoor air_quality and using little ventilation. The authors measure a typical department store and analyze the measurement. The conclusion as well as the opinion is given, which argues for improving the efficiency of new_wind.     

Wide mapping of a T-DNA insertion site in oilseed rape using bulk segregant analysis and comparative mapping

Doubled haploid oilseed rape lines segregating for a transgene inducing herbicide resistance (bar gene) were investigated for the wide mapping of the T-DNA insertion site. Bulk segregant analysis using presence/absence and intensity polymorphisms between the bulks, as well as comparative mapping with a linkage group deriving from another cross, led to the identification of 11 random amplified polymorphic DNA (RAPD) markers tightly or loosely linked to the bar gene. Ten RAPD loci out of 11 were located on the same side of the bar locus, strongly suggesting that the T-DNA integrated in a telomeric or subtelomeric position. The eleventh RAPD marker exhibited a strong segregation distortion, which could be the result of a heteroduplex formation. Comparison of the linkage groups obtained from the two crosses showed different recombination rates between markers, possibly reflecting differences in parental genetic backgrounds. Consequences and potential applications in transgene dispersal safety assessment studies are discussed.     

The integration and insertion site of GbVe1 gene in the genome of transgenic cotton (Gossypium hirsutum)

[Objective] The aim of this study was to obtain the flanking sequences of T-DNA in the transgenic cotton containing a GbVe1 over-expression cassette. [Method] The T-DNA insertion copy number in the transgenic GbVe1 cotton was analyzed by southern blot. Flanking sequences of the transgenic lines with putative single T-DNA insertion copy were obtained using high-efficiency Thermal asymmetric interlaced polymerase chain reaction (hiTAIL-PCR). The T-DNA insertion sites were further confirmed by PCR with specific primers. [Result] RB-flanking sequences (119-1 018 bp) and LB-flanking sequences (243-516 bp) were obtained from three transgenic lines with low copy number of T-DNA insertion. The AT content was more than 63% in these flanking sequences. A same single insertion site in the intron of Gohir.D01G157600.1 was found in the two transgenic lines 7/100826-152 and 12/100826-393, while two separated insertion sites, one also in the intron of Gohir.-D01G157600.1 and the other in the intergenic region of A12 chromosome, were found in the transgenic line 1/w-ch14. A deletion of 21 bp was found in the insertion site in the intron of Gohir.D01G157600.1. The T-DNA insertion in the intron of Gohir.D01G157600.1 was further confirmed by the specific PCR. [Conclusion] The flanking sequences of T-DNA in the transgenic GbVe1 cotton were obtained and the specific transformation event in the intron of Gohir.D01G157600.1 was further confirmed by PCR.     

沿海地区耕地集约利用与生态服务价值动态变化及相关性分析

沿海地区既是人类活动剧烈区又是生态环境敏感脆弱区,其耕地利用变化与生态环境效应研究亟待加强。以江苏沿海为研究区域,基于耕地集约利用和生态服务价值模型,估算沿海19个县市耕地集约利用水平与生态服务价值,分析其时空演变特征,探讨沿海地区耕地集约利用与生态服务价值的动态变化特征。结果显示：（1）2000-2010年江苏沿海19个县市耕地集约利用水平显著提高,2000年、2005年、2010年其平均值分别为0.181,0.251和0.372,空间格局变化特征显著,Ⅰ级区范围呈缩小趋势,Ⅱ级区和Ⅲ级区范围有所增加。（2）耕地生态服务价值呈波动变化,2000年、2005年、2010年其耕地生态服务价值平均值分别为1.68×109元,1.58×109元,1.55×109元,苏北地区耕地服务价值呈下降趋势,苏南地区呈上升趋势,耕地生态服务价值Ⅰ级区呈零散分布,Ⅱ级区范围在扩大,Ⅲ级区范围呈减少态趋势。（3）江苏沿海地区耕地集约利用水平与生态服务价值呈显著负相关,不同典型年份其相关系数分别为-0.700、-0.662和-0.682,不同经济梯度下其相关系数存在明显差异,经济发达的苏南地区相关系数值大于苏北地区,苏南地区相关系数呈下降趋,而苏北地区呈上升趋于。     

贝母属植物叶绿体基因组候选SNP位点应用分析

贝母属植物是中国中药材的宝库,由于形态上难以区分,在民间应用、临床应用和中药市场中还存在混淆应用、以次充好的现象,急需开发基于新型分子标记位点的精准检测方法。本研究应用多重序列比对、SNP筛选等生物信息学分析手段,对贝母属15种植物28条叶绿体基因组序列进行分析。结果发现,贝母属叶绿体基因组DNA同源性达97.22%,通过分析共发现SNP位点5879个,其中川贝母类鉴别候选位点71个,川贝母鉴别候选位点4个,瓦布贝母鉴别候选位点37个,太白贝母鉴别候选位点147个,浙贝母鉴别候选位点91个,湖北贝母鉴别候选位点271个,平贝母鉴别候选位点1393个,伊贝母鉴别候选位点89个。本研究还对SNP位点在精准鉴定、精确定量应用方面进行了讨论,可为川贝母中药资源鉴定提供技术支撑。     

滨海盐碱地不同抗虫棉品种的产量和熟相及去早果枝的效应

 为探讨不同抗虫棉品种在滨海盐碱地的产量和熟相及其对去早果枝的反应,以早发型（鲁棉研17、21）、晚发型（鲁棉研18、22）抗虫棉和两者的F₁杂交种（01H00、01H01）为材料,于2006－2007年在东营市盐碱棉田进行了试验观察。结果表明,早发型和晚发型抗虫棉品种间的早熟性和熟相存在显著差异,两者的杂交种在2006和2007年分别比常规种增产5.7%~14.5%和4.7%~6.8%,并表现出较好的熟相。早发型常规抗虫棉去早果枝增产5.6%~8.4%,早衰得到延缓;晚发型常规抗虫棉去早果枝减产9.3%~13.1%,表现晚熟。利用不同熟相类型的常规抗虫棉配置杂交组合以及对早发抗虫棉品种去早果枝都是延缓早衰、提高盐碱地棉花产量的有效途径。     

浅圆仓充氮气调技术实仓应用探究

通过对浅圆仓采用不同方法进行充氮试验,探索最佳的浅圆仓充氮气调储粮工艺。试验结果表明,采用上充下排方式,先以设备允许的最大流量(流量165m~3/h,浓度95%)充入氮气,当仓内氮气浓度接近95%时,再逐步提高设备氮气浓度至99%(流量90m~3/h),此工艺相较其他方法充氮速率略高,能耗相对略低,仓内氮气分布均匀。     

滨海盐碱地改良研究现状及展望

在中国东部耕地资源紧缺、人地供需失衡的背景下,迫切需要增加耕地,保证"占补平衡".滨海盐碱地作为一类潜在的土地后备资源,其合理开发利用对缓解土地资源不足的压力、提高农业水资源利用效率和生态系统稳定性具有重要的战略与现实意义.本文介绍了滨海盐碱地的资源现状及主要特点,从物理、化学、水利工程、生物4个方面总结了当前滨海盐碱...