甘肃健康马属动物18项生理生化指标的测定

在大家畜急腹症外科临床研究的预备试验中,对甘肃武威地区健康马、骡、驴的PCV,TP,血糖,血液乳酸,血清钾、钠、氯和皮肤弹力,毛细血管再充盈等18项生理生化指标进行了检测。结果与国内外有关文献报导的数值不尽相同,血液乳酸正常值(马26.25±0.87mg％)大于国外文献报导值(10.0～12.0mg％),而且差异显著。     

人工神经网络在生物化学中的应用

本文论述了生物化学中模式识别、定量构效关系、化合物结构解析等运用人工神经网络进行计算的方法。     

植物淹水胁迫响应的研究进展

在概述植物对淹水胁迫各种适应方式的基础上,重点从质外体障碍的形成和根系的向氧性生长,发酵途径、厌氧多肽、植物激素及低氧锻炼等方面,对近年来植物抗淹水胁迫适应性的研究进展作了简要评述.     

电麻醉对西伯利亚鲟幼鱼血液生化指标的影响

设置5个麻醉组(15～35 V)与1个对照组(0 V),研究直流电麻醉对西伯利亚鲟幼鱼血液13项生化指标的影响.随着电压强度升高,西伯利亚鲟麻醉组总蛋白与白蛋白质量浓度逐渐升高,35 V组总蛋白质量浓度显著高于15 V组与20 V组.高密度脂蛋白、低密度脂蛋白和甘油三酯浓度在15 V组中最低,在35 V组最高,35 V...     

丁草胺对不同秧苗素质水稻生理生化特性的影响

通过盆栽试验,探讨了丁草胺对不同秧苗素质水稻生理生化指标的影响.丁草胺处理后叶片的光合作用速率、叶绿素含量和根系活力下降,而GSH含量和SOD活性上升.水稻弱苗对丁草胺的敏感性高于壮苗.     

蝴蝶滞育研究进展

介绍了近年来蝴蝶滞育虫态、光敏感时期、光周期和温度等环境因素对滞育的影响、滞育期间的形态结构和生理生化变化、滞育的神经内分泌调控和分子生物学等方面的研究概况。指出,目前蝴蝶滞育研究主要集中在外界环境对滞育的影响上,在生理生化和内分泌机制等方面的研究相对薄弱,而在分子生物学方面的研究报道极少。滞育的生理生化机制、神经内分泌调控、分子机制和滞育应用研究,将成为今后蝴蝶滞育研究的热点;一些观赏价值较高的蝴蝶将成为今后研究的重点。充足稳定的实验蝴蝶难以获得,是目前研究蝴蝶滞育的一个难点。蝴蝶养殖业的发展将有利于推动蝴蝶滞育研究。参63     

Determination of angiotensin I-converting enzyme activity in equine blood: lack of agreement between methods of analysis

Angiotensin-I converting enzyme (ACE) is a key regulator of blood pressure, electrolytes and fluid homeostasis through conversion of angiotensin I into angiotensin II. Recently, a genetic polymorphism of the ACE gene, which accounts for 47% of the variation of ACE activity in blood, has been advocated as a biomarker of athletic aptitude. Different methods of analysis and determination of ACE activity in plasma have been used in human and equine research without a consensus of a "gold standard" method. Different methods have often been used interchangeably or cited as being comparable in the existing literature; however, the actual agreement between assays has not been investigated. Therefore, in this study, we evaluated the level of agreement between three different assays using equine plasma obtained from 29 horses. Two spectrophotometric assays using Furylacryloyl-phenylalanyl-glycyl-glycine as substrate and one fluorimetric assay utilizing o-aminobenzoic acid-FRK-(Dnp)P-OH were employed. The results revealed that the measurements from the different assays were not in agreement, indicating that the methods should not be used interchangeably for measurement of equine ACE activity. Rather, a single method of analysis should be adopted to achieve comparable results and critical appraisal of the literature is needed when attempting to compare results obtained from different assays.     

草莓枯萎病菌抗多菌灵突变体ZY-R的生理生化特性研究

为明确抗多菌灵草莓枯萎病菌(ZY-R)菌株的生理生化特性,分析其可能的抗性机制,以课题组获得的抗多菌灵达53.91倍的ZY-R菌株及其敏感菌株(ZY)为研究材料,比较了ZY-R与ZY在苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)活力及相对渗率等方面的差异。结果表明:以清水和不同浓度多菌灵处理两菌株1.5~24 h后,ZY-R体内的POD和PAL活力始终高于ZY的;敏、抗菌株间POD活力的差异远大于PAL。在处理时间内,PAL活力先上升后下降,6 h达到最高值;而POD活力一直处于上升状态,24 h达到最高值,且敏、抗菌株之间的差异达到最大,25 mg/L条件下ZY-R的POD活力为ZY的2.26倍;此外,与ZY相比,ZY-R可以渗出更多的电解质。     

低温胁迫下番石榴叶片生理生化变化的探讨

通过不同低温对台湾引进的珍珠番石榴、无籽番石榴及福建省乡土番石榴的处理 ,测定其在低温胁迫下叶片若干生理生化变化 ,以探求番石榴不同品种的抗寒性与忍受的临界低温。结果表明 ,番石榴忍受的临界低温为 -3～ -4℃ ,因品种和低温持续时间而异。番石榴不同品种间耐寒性差异与叶片束缚水 自由水比值以及可溶性蛋白质、可溶性糖含量相关。乡土番石榴的抗寒性高于从台湾引进的两个品种。     

COMPARATIVE EVALUATION OF HYDROPIC DEGENERATION OF THE LIVER IN DAIRY CATTLE THROUGH BIOCHEMISTRY, ULTRASONOGRAPHY AND DIGITAL ANALYSIS

Ultrasonography of the liver of 181 Holstein-Friesian cows was performed and blood samples were collected for analysis. The hepatic ultrasonograms were evaluated and the echoes were analyzed digitally. After slaughter, liver specimens were taken and examined histopathologically. Of the 181 animals, 120 had a normal liver and 61 had hydropic degeneration of the liver, diagnosed through histopathologic examination. Diagnostic accuracy rates for hydropic degeneration were determined based on the following test positive conditions: a) for biochemical analysis—high levels of aspartate aminotransferase, alanine aminotransferase, total bilirubin and non-esterified fatty acids; b) for ultrasonography—presence of dark pattern and blurring of edges; and c) for digital analysis—low echo means at 1 cm and 3 cm from the hepatic surface. Digital analysis had the highest overall specificity, accuracy and positive predictive values for hydropic degeneration, followed by ultrasonography. The results suggest that ultrasonography and digital analysis of hepatic ultrasonograms can be used for diagnosis of hydropic degeneration of the liver in place of biochemical analysis.