Parentage assignment of stocked black sea bream <Emphasis Type="Italic">Acanthopagrus schlegelii</Emphasis> in Hiroshima Bay using microsatellite DNA markers

The genetic contribution of 51 broodstock, comprising 29 females and 22 males, reared at Hiroshima City Marine Products Promotion Center for the production of stocked black sea bream was monitored during two consecutive years using seven microsatellite DNA loci. The high discrimination ability of these markers was reflected in the polymorphic identification content (PIC=0.831), the exclusion probability (Q≈1), and the low probability of identity index (I=3.635⁻¹⁰). The total number of breeders contributing to the mating process was estimated at 32 (62.7%) in 2000 and 30 (58.8%) in 2001. On pedigree reconstruction, 69.3% of the offspring were successfully assigned to a single broodstock pair. Loss of alleles accounted for 16.9% during seed production; nevertheless, 90.9% of males and 69.0% of females participated in the mating process. Based on microsatellite genetic tagging, 58.9% of the fish sampled during the two months after release were identified as hatchery stock, presenting no significant differences from wild conspecifics in either fork length or body weight.     

Considerations of stock structure of yellowfin tuna (Thunnus albacares) in the Indian Ocean based on fishery data

Yellowfin stock structure in the Indian Ocean was studied by using industrial tuna longline fishery data. Three types of test variables were used to detect stock structure, i.e., CPUE, age-specific CPUE, and coefficient of variation for size. Time-series data of test variables were compiled for six sub-areas that were arranged by dividing the whole region systematically along longitude lines every 20 degrees. Then time-series data were smoothed by moving averages, and regressed by simple models. Patterns of time-series trends were graphically and statistically compared to classify homogeneous sub-area groups. Two assumptions were (a) that homogeneous stocks exist longitudinally and overlap in adjacent waters, and (b) that test variables within homogeneous sub-area groups are equally affected, and hence patterns of the time-series trends are similar. After graphical screening for significant sub-area groups, analysis of covariance was applied to test homogeneity of regression parameters representing patterns of the time-series trends. By classifying homogeneous sub-area groups, stock structures were determined at the P <0.05 and P <0.50 levels. The P<0.50 level was recognized as a useful criterion for ‘weak’ test variables since masked or vague structures at the P <0.05 level were likely cleared at this level in many cases. Results of this study and past stock structure studies were reviewed and compared. It was concluded that there are two major and two minor stocks of yellowfin tuna. The two major stocks (the western and the eastern) are located at 40^o-90^oE and 70^o-130^oE respectively. The minor stocks are the far western and the far eastern stocks (the latter possibly being a part of the Pacific stock), which are located westward of 40^oE and eastward of 110^oE respectively. Neighboring stocks are intermingled in adjacent waters.     

An examination of the use of angling data to estimate total returns of Atlantic salmon, Salmo salar, to two rivers in Newfoundland, Canada

Conservation spawning requirements (limits) for Atlantic salmon, Salmo salar L., have been developed for a number of rivers in Newfoundland. Status of stocks is evaluated against these limits and scientific advice and recommendations on levels of recreational catch provided to resource managers. A critical factor in this process is the determination of total returns. This has been accomplished for 30–40% of the rivers in question over the years through the use of fish counting fences and traps installed in fishways. In Newfoundland, it is not logistically or financially possible to operate fish counting facilities in all of the approximately 200 Atlantic salmon rivers. Thus angling catch data have been used as an alternative means of estimating total returns to rivers without counting facilities. Estimates of total returns were three to four times higher than the actual values for one of the two rivers studied and deviated from the actual by as much as 60% for the other. Management implications of the approach are discussed.     

Discrimination of the natal origin of young-of-the-year Allis shad (Alosa alosa) in the Garonne–Dordogne basin (south-west France) using otolith chemistry

Abstract –  The otolith composition of age 0+ Allis shad ( Alosa alosa ) captured in two rivers in the Gironde system in south-west France, the Dordogne and the Garonne, was analysed to establish the natal origin of the fish. Inductively coupled plasma mass spectrometry analyses were used to take measurements of 15 isotopes, among which Co, Fe, Sr and Rb were the most important elements in the discrimination process. It was possible to reclassify each sampled fish into its natal river basin (Garonne or Dordogne) with a high degree of accuracy. Linear discriminant analysis correctly reclassified 91.1% of the Garonne samples and 87.8% of the Dordogne samples (mean success in reclassification in 20 runs ± 1 SD: 89.21 ± 8.6%). Artificial neural networks correctly reclassified 90.1% of the Garonne samples and 81.2% of the Dordogne samples (mean success in reclassification after 1000 training cycles ±1 SD: 85.6 ± 9.1%). Using these tools the present results show that it is possible to assign a natal origin to juvenile shad that hatched in the Dordogne–Garonne basin based on the trace elements in the otolith.     

南方山地森林小班蓄积量调查方法的对比分析

以南方山地森林为研究对象,选取有代表性的4个小班,分别采用角规抽样调查法、中心样圆法、样圆群法、六株木法、垂直样带法、标准地法共6种方法对小班蓄积量进行测定,并从6种方法的调查精度、误差大小、工作量及工具和仪器的难易程度等方面对小班蓄积量进行了对比分析,认为角规抽样调查法是最适合南方山地森林小班蓄积量的调查方法。     

建立相似林分样木因子回归模型推算采伐木蓄积

在安化县杉木人工林相似林分内设立样地,测量样地对角线上26株林木的地径、胸径和树高,用Excel软件建立地径与胸径、胸径与树高的回归模型,再测量采伐木地径,用回归模型预测采伐木的胸径和树高,推算采伐木蓄积量。同时,采用地径一元立木材积表法测算林木蓄积量,并与采伐作业设计的二元立木材积表法测算的林木蓄积量相比较。结果表明:采用建立相似林分样木因子回归模型推算采伐木蓄积量方法接近采伐作业设计的蓄积量。     

海洋渔业生物增殖放流效果评估研究进展

增殖放流是重要的海洋渔业资源恢复措施,目前该项技术在世界各国大量开展。增殖放流效果评估是 放流工作体系的核心环节之一,根据评估结果,可以不断改进放流技术,使放流效果不断改善。从增殖效果、生态效 果、社会效果3 个方面对国内外海洋渔业生物放流效果评估的主要研究方法和成果进行论述,并对该领域未来的研 究趋势进行展望,可以为放流效果评估工作的高效开展、放流技术的改进提供参考。     

双向选择对团头鲂生化遗传变异的影响↑（*）

本文报导双向选择对团头鲂生化遗传变异的影响。试验材料鱼包括来自淤泥湖的原种，１９８６－１９９４年群体选育的第四代（“选育 Ｆ４”），以及１９９１、１９９４年连续兄妹杂交的第二代（“近交Ｆ２”）。计检测了８种同工酶，１９个位点。三群体的多态座位比例一致（５．２６％），但平均杂合度趋异。“选育Ｆ４”平均杂合度最高（０．０２１３），“近交Ｆ２”最低（０．０１７４）。经４代选育，平均杂合度增加４％，而经２代近交，平均杂合度降低１５％。     

仁寿县种草养畜生产现状调查报告

仁寿县 (四川农区种草养畜生产发展的重点县 )种草养羊、养牛、养兔、养鹅、养猪发展变化很大 ,是耗粮型畜牧业向节粮型畜牧业转变的典型代表。要加强搞好草畜配套 ,以畜定草工作。大力发展牧草品种、数量和种植面积。