Erwinia amylovora populations resistant to oxolinic acid in Israel: prevalence, persistence and fitness

Oxolinic acid (OA) has been the only bactericide used against fire blight in pear and quince orchards in Israel since 1998. OA-resistant Erwinia amylovora strains (Ea-OA^R) were detected in several orchards in two restricted areas in the northern Galilee region during 1999–2001. In the following years, resistant strains could not be detected in some of these locations. Documenting the fate of Ea-OA^R strains in commercial orchards at eight sites in northern Israel during 2000/03 revealed that the resistant population appeared irrespective of the number of sprays applied and the severity of the disease. The persistence of the Ea-OA^R populations varied from site to site, ranging from 4 to 20 months; these differences could be attributed to the fire blight management activities of growers. Comparative studies on the fitness of Ea-OA^R and E. amylovora strains sensitive to OA (Ea-OA^S) were conducted in vitro and in planta using two strains of each group. In four of the six comparisons, disease incidence on detached blossoms inoculated with Ea-OA^S was significantly higher than that on blossoms inoculated with Ea-OA^R. In two experiments conducted on 8-year-old pear trees grown under netting, the colonization of Ea-OA^S in blossoms, annual shoots and perennial spurs was significantly higher than that of the Ea-OA^R. In two experiments conducted on 2-year-old trees grown under netting in an experimental station, the incidence of shoots exhibiting fire blight symptoms and the rate of symptom progress within the branches were significantly higher in trees inoculated with Ea-OA^S than in those inoculated with Ea-OA^R. The results of this study suggest that OA-resistant E. amylovora strains have lower fitness than wild-type strains. These findings may have implications for fire blight management.     

Efficacy of oxolinic acid and other bactericides in suppression ofErwinia amylovora in pear orchards in Israel

The efficacy of oxolinic acid (at 200 and 300 μg a.i./l) and of several antibiotic compounds (streptomycin sulfate at 100 μg a.i./l, glycocide B at 700 μg a.i./l, kasugamycin at 80 μg a.i./l and gentamicin sulfate at 30 and 60 μg a.i./l) againstErwinia amylovora, the causal agent of fire blight in pears, was evaluated in 43 orchard experiments in 1997–2000 in Israel. In addition to the above orchard experiments, the efficacy of the bactericides was tested in live experiments with artificial inoculation. Natural fire blight symptoms were observed in 16 of the 43 experiments; in 13 of them, disease intensity and its distribution among the experimental plots provided a basis for data analysis, leading to reliable conclusions concerning the efficacy of the tested bactericides. Oxolinic acid at 300 μg a.i./l was highly effective againstE. amylovora and reduced disease severity significantly in all experiments, as compared with the untreated plots; however, a concentration of 200 μg a.i./l was not effective in some cases. Among the tested antibiotics, only gentamicin sulfate was as effective as oxolinic acid. Results of the artificial inoculation experiments corroborated those obtained in the naturally infected orchards. The pre-infection activity of oxolinic acid was determined on blossom clusters that were sprayed with the bactericide before inoculation. Control efficacy on blossom clusters sprayed 1–4 days before inoculation ranged from 68% to 80%, a level which did not differ significantly from that observed on blossom clusters sprayed on the day of inoculation (80% control). The postinfection activity of oxolinic acid was determined on blossom clusters that were sprayed with the compound after inoculation. Oxolinic acid was as effective when applied 1 or 2 days after inoculation as when it was applied on the day of inoculation; however, application of the bactericide 3 days after inoculation no longer resulted in significant disease suppression. Oxolinic acid has been used commercially in Israel since 1998 with appreciable success. Contribution No. 533-00 from the Agricultural Research Organization     

Fire-induced changes in northern Patagonian landscapes

In northern Patagonia, Argentina we quantify changes in fire frequency along a gradient from mesic Nothofagus dombeyi forest to xeric woodlands of Austrocedrus chilensis at the steppe ecotone, and we examine patterns of vegetation change coincident with the changes in fire regimes across a range of spatial scales. At a regional scale changes in land cover types are documented by comparing 1:250000 scale cover type maps from 1913 and 1985. Changes in landscape structure are analyzed by comparing vegetation patterns on 1:24000 scale aerial photographs taken in 1940 and 1970. Fire frequency peaked in the late nineteenth-century due to widespread burning and clearing of forests by European settlers late in the century. Subsequently, fire frequency declined dramatically about 1910 due to the cessation of intentional fires and has remained low due to increasingly effective fire exclusion. At a regional scale there has been a dramatic increase during the twentieth century in the proportion of forest cover relative to areas mapped as recent burns or shrublands in 1913. Remnant forest patches that survived the widespread late-nineteenth century burning have coalesced to form more continuous forest covers, and formerly continuous areas of shrublands have become dissected by forest. Under reduced fire frequency there has been a shift in dominance from short-lived resprouting species (mostly shrubs) towards longer-lived species and obligate seed-dispersers such as Austrocedrus chilensis and Nothofagus dombeyi. Due to limited seed dispersal of these tree species, the spatial configuration of remnant forest patches plays a key role in subsequent changes in landscape pattern.     

库鄯线INERGEN气体自动灭火系统

介绍了库鄯输油道采用的ＩＮＥＲＧＥＮ气体自动灭火系统的组成、特点、工艺及操作主要设备参数以及测试方法等，对该系统的维护及保养提出了几点建议，该系统的建立可有效地保护库鄯输油管道首末站的站控室，通信机房、ＵＰＳ间及减压站的柴油发电机房、配电室，站控室，通信机房及ＵＰＳ间等。     

福建省1990-2009年森林火灾减灾投资效益分析

根据福建省1990-2009年森林火灾统计资料，以减少生态、经济和社会效益损失为目的，运用灾害经济学理论对相关数据进行实证研究．结果表明：福建省近20年来虽然不断提高森林防火的投入，但在减灾效益方面收到的效果却仍不理想．造成森林火灾减灾效益较低的主要原因分别是重大森林火灾发生率提高、森林单位面积综合价值提升及气候变化异常．     

浅谈瞭望塔在林火监测体系中的应用

针对五常市林业局瞭望塔布局和选址情况，论述了瞭望塔建设应本着科学规划，合理布局，注重实效，善于管理的原则建设和应用管理。     

林火对长白山自然保护区风灾区土壤性质的影响

以2012年长白山国家级自然保护区西坡风灾区森林火灾为研究背景，采用常规土壤分析方法研究了不同火烧强度下土壤理化性质的变化情况．结果表明：火烧后土壤容重和土壤含水率随火烧强度的增强显著下降；0～6cm土壤pH，土壤有机质，土壤全C、全N、速效P、速效K质量分数经轻度和中度火烧后均呈显著增加趋势；在火烧强度因子的控制下，对各养分质量分数进行的偏相关分析显示，除土壤pH外，其他各养分间存在显著的相关性．     

Effect of Freezing Snow Disaster on Forest Fuel and Fire Behavior in Southern China

After freezing snow disaster,stands of Pinus massoniana,Cunninghamia lanceolata. Liquidambar formosana,Cinnamomum camphora and Phyllostachys pubescens and new burned areas in Hunan Province were surveyed by setting field plots.Moisture content and fuel load were measured by sampling and fire behavior(such as rate of fire spread) was calculated.The results showed that fuel load got doubled instantly,fuel thickness enhanced,fuel continuity swelled and water content of fuel decreased as shrubs and herbs die...     

瓯海区森林火灾发生规律及其对策

根据瓯海区1990～1996年森林火灾发生的实际情况，探索其发生的规律，提出相应的对策。瓯海区森林火灾全年均有发生，主要集中在春季、最多发生在4月；从一天来看，最多发生在12～14时；引起火灾最多的人为因子是上坟烧银纸；肇事人年龄段，最多是51～60岁。因此，要搞好森林防火工作，应分别对象做好宣传工作，严禁带火源上山。     

松嫩草原火干扰状况研究

松嫩草原自然状态下火周期为４年，最易着火的季节—时间是每年４月和１０月，以４月份最为敏感。松嫩草原火提高的地下０．５ｃｍ处土壤温度与燃掉的可燃物量成直线正相关。燃烧指数以每年的４月份最高，大于６０，形成炙热速进火。草原火管理工作中，应着重加强对每年４月份的警戒。