Mechlorethamine, procarbazine and prednisone for the treatment of resistant lymphoma in dogs

Forty-one dogs with resistant lymphoma were treated with a modified MOPP (mechlorethamine, vincristine, procarbazine and prednisone) protocol (MPP [mechlorethamine, procarbazine and prednisone] administered on a 21-day cycle, shortened from the 28-day MOPP cycle). The overall response rate to MPP was 34% for a median of 56 days (95% confidence interval 30–238). Seventeen percent of dogs had a complete response for a median duration of 238 days, 17% had a partial response for a median of 56 days and 32% had stable disease for a median of 24 days. Histological grade or cell morphology on cytology was associated with response. Minimal toxicity was observed with the MPP protocol, suggesting that further dose intensification or addition of another chemotherapeutic agent would be possible.     

A dexamethasone,melphalan, actinomycin‐D and cytarabine chemotherapy protocol as a rescue treatment for feline lymphoma

Nineteen cats with relapsed high‐grade/large‐cell lymphoma were treated with dexamethasone, melphalan, actinomycin‐D and cytarabine (DMAC). All cats had received Cyclophosphamide, Vincristine, Prednisolone (COP) as first‐line chemotherapy and most cats had received at least 2 prior rescue agents with 14 of 19 having received both epirubicin and lomustine. Five cats (26%) exhibited a response (defined as an improvement or resolution of tumour‐associated clinical signs/tumour volume, or complete/partial response) to chemotherapy though no patients received more than 2 cycles of DMAC. Most cats tolerated the protocol well though 3 patients exhibited Veterinary Cooperative Oncology Group (VCOG) grade 4 neutropenia and 1 patient exhibited grade 4 thrombocytopenia. The median progression‐free survival and overall survival from starting DMAC were 14 and 17 days respectively. There is still an unmet need for successful rescue chemotherapy protocol for cats with relapsed lymphoma. [Correction added on 02 November 2017, after first online publication: The expansion for the term DMAC was previously incorrect and has been corrected in this current version.]     

不同盐胁迫对高羊茅种子萌发的影响

用不同浓度的NaCl,Na2CO3,Na2SO4 ,NaCl和Na2CO3 的混合液以及NaCl、Na2CO3和Na2SO4的混合液分别处理高羊茅（Festuca arundinacea）种子,研究不同盐分胁迫对高羊茅种子发芽率、发芽势、发芽指数、活力指数以及幼苗根长和苗高的影响。结果表明,低浓度盐处理对高羊茅种子的萌发影响不大,高浓度盐处理显著抑制了种子的萌发。随着盐浓度的升高,高羊茅种子的发芽率、发芽势、发芽指数和活力指数均呈下降趋势,且对幼苗生长和幼根生长的抑制作用加强。高羊茅种子对不同类型盐的耐受性各异,不同盐分对种子萌发的抑制程度为Na2SO4<混合盐(NaCl+Na2CO3+Na2SO4)相似文献   

含蒙脱土和多糖的保水剂对高羊茅品种可奇思种子萌发及生长的影响

采用正交实验法在室温下合成了含有天然产物蒙脱土（MMT）和羧甲基纤维素钠（CMC）的系列环境友好型保水剂,研究了不同保水剂组成和合成条件对高羊茅品种可奇思（简称可奇思）种子发芽及生长指标的影响规律。结果表明,试验合成的保水剂能显著提高可奇思的发芽能力和植株的生长指标,天然组分MMT和CMC含量对其发芽和植株生长影响显著。用性能最佳的保水剂处理可奇思种子,其发芽率、发芽指数、活力指数、总干重、总根长和总分蘖数分别比空白对照试样提高了61．6％、80．5％、125％、102％、67．6％和68．6％,比市售合成类保水剂对照试样提高了4．50倍、5．50倍、7．60倍、6．88倍、3．88倍和3．92倍,这对提高可奇思种子发芽能力和促进其植株生长具有重要意义,对该保水剂在可奇思种子发芽中的应用提供了试验依据,     

I类新城疫病毒NDV08-004株微型基因组的构建及其功能鉴定

目的本研究旨在构建Ⅰ类新城疫病毒NDV08-004的微型基因组和辅助质粒,为构建病毒拯救体系奠定基础。方法根据Ⅰ类新城疫病毒NDV08-004的全基因组序列,采用SOE方法将增强绿色荧光蛋白基因(eGFP)插入NDV08-004的3’-Leader和5’-Trailer区域之间后反向插入转录载体pOLTV5中,构建了NDV08-004的微型基因组pLGT-03。将pLGT-03转染辅助病毒NDV08-004感染的表达T7RNA聚合酶BSRT7/5细胞进行功能鉴定。采用RT-PCR将NDV08-004的NP、P和L蛋白基因亚克隆入表达载体pCI-neo中,分别构建了三个辅助质粒pCI-NP、pCI-P、pCI-L。通过构建的微型基因组pLGT-03和三个辅助质粒按照一定的比例共转染BSRT7/5细胞来验证辅助质粒的功能。结果微型基因组pLGT-03转染辅助病毒感染的BSRT7/5细胞后可见明显荧光,表明微型基因组构建成功。微型基因组和辅助质粒共转染可见荧光表达,表明三个辅助质粒均具有相应的功能。结论表明微型基因组和辅助质粒均具有相应的功能,为建立NDV08-004的反向遗传操作平台奠定了基础。     

Production of interspecific hybrids between Alstroemeria pelegrina L. var. rosea and A. magenta Bayer by ovule culture

Interspecific hybrids were efficiently produced in the cross-incompatible combination between Alstroemeria pelegrina L. var. rosea and A. magenta Bayer by culturing immature ovules with placenta 7–14 days after pollination on 2 g/l Gelrite-solidified MS medium containing 3% (w/v)sucrose. The plants showed intermediate characteristics between the parents and their hybridity was confirmed by karyotype and DNA analyses. The mean number of chromosome association per PMC at metaphase I was 2.60I+6.70II, pollen stainability was20.8%, and they produced viable seeds after self-pollination. Furthermore, mature plants were obtained when the hybrids were backcrossed as male parents with both the parents. The backcross-progeny from A. pelegrina var. rosea × hybrids exhibited 3.8 to 79.7% pollen stainability and that from A. magenta × hybrids 78.8 to 98.3%. Almost all of these plants produced viable seeds after self-pollination, which implies that they can beutilized for breeding of novel cultivars of Alstroemeria. This revised version was published online in July 2006 with corrections to the Cover Date.     

槭属植物远缘杂交初探

以美国红枫为母本,我国原产的元宝枫为父本,采用直接授粉法、切除柱头法、切除柱头加化学试剂法3种方法在温室条件下,用超低温保存的元宝枫花粉对带有花芽的离体美国红枫枝条进行授粉实验;子房发育约20~25d之后,分别采集经不同处理的子房对其进行表面灭菌处理后,接种于1/2MS+6-BA(0.01mg/L)+NAA(0.1mg/L)的培养基上进行胚抢救培养。结果表明:含水量为12.2%的花粉经超低温保存一年之后,保持着与新鲜花粉相当的活力。3种不同的授粉方法中,生长情况最好的为直接授粉法,切除柱头加化学试剂法次之,而切除柱头发效果最差。通过胚抢救技术获得了一定量的杂种后代株系。     

部分菊属植物种间杂种难稔性及其克服

菊花(Dendranthema morifolium(Ramat.) Tzvel.)近缘种属植物中具有许多栽培菊花所缺乏的优良性状,如抗逆性、抗病虫性,通过远缘杂交结合幼胚拯救技术能有效克服菊属(Dendranthema (DC.)Des Moul.)种间障碍,进行菊花种质创新和品种改良[1].远缘杂交亲本由于缺少可识别遗传信息及染色体同源性差异,种间杂种可能具有难稔性[2].