夏秋季中街山列岛养殖海域营养盐水平及有机污染状况评价

根据2009年8月和10月夏、秋季节中街山养殖区调查数据,对该海域的营养盐水平及有机污染现状进行了分析评价。结果表明：中街山列岛养殖区无机氮夏季含量较高,16.7%的站位超四类水质标准,秋季明显下降,符合二类水质标准。活性磷酸盐含量夏、秋季无明显差异,而硅酸盐秋季较夏季明显升高。磷酸盐为该海域的限制因素,夏季尤为明显。该海域已呈富营养化状态,有机污染也已达到轻度污染水平,且夏季高于秋季。     

咸宁市桂花资源地理标志知识产权现状及对策

介绍了我国地理标志保护概况及桂花资源的地理标志知识产权保护概况,分析了咸宁市桂花资源的优势,并从地理标志特征、地理标志保护、非物质文化遗产等方面,探讨咸宁县桂花及其相关特产的地理标志保护对策。     

Early-sowing,high-yielding and high-quality cultivation techniques of fall-planting balsam pear in the hilly area

利用山区中高海拔特有的地理气候资源优势,采用秋苦瓜早播技术,包括品种选择、播期安排、培育壮苗、合理密植、田间管理等技术,达到提早上市、提高经济效益的目的。     

发展油橄榄产业势在必行

我国用占世界7%的土地（耕地占3%）解决了占世界1/5人口的“吃饭”问题,但人口增加,耕地减少的趋势近期难以逆转,“粮食安全”仍不容忽视。大力发展油橄榄这种产油量高的木本油料替代占用耕地较多的草本油料,应是解决耕地紧张的一条出路。油橄榄在我国的适生范围较广,主要集中于长江上游的云、贵高原,金沙江流域、三峡低山河谷和秦巴山地区,其中有近60%是贫瘠的丘陵山区,属国家和地方专项资金扶持的贫困县,也是“三峡”水利设施工程（航运、发电）的水土保持植树造林地区。在这一范围内,因地制宜发展油橄榄生产,具有开发山区,综合治理长江,促进贫困地区经济发展,改善生态环境的多重效益。     

我国油橄榄中试园土壤条件及其营养指标的研究

1983—1987年对湖北省武昌、宜昌、当阳县和陕西省城固县油橄榄中试园土壤与营养状况进行了研究。结果表明:油橄榄生长较好的(一类树下)土壤条件是pH7—8,CaCO_3>5％,有机质>1.8％,速效N>74.4—87.4mg/kg土,速效P>40mg/kg土,速效K>200mg/kg土,交换性Ca103—181mmol/kg土,交换性Mg24—34mmol/kg土。生长结实好的一类树叶片营养指标为N2.0—2.5％,P_2O_5 0.14％,K_2O 1.5％,CaO 4.0％,MgO 0.8％。通过分析建立了预测产量和施肥控制的回归方程。     

Supplemental iron and phosphorus increase dietary inclusion of cottonseed and soybean meal in olive flounder (Paralichthys olivaceus)

A long‐term feeding experiment was conducted to investigate the use of cottonseed and soybean meal (CS) with iron and phosphorus supplements in diets for olive flounder (Paralichthys olivaceus). Olive flounder with an initial average size of 28.5 ± 0.35 g (mean ± SD) were divided into 15 groups (three tanks per dietary treatment) and fed 480 g kg^?1 crude protein diets in which each of five isonitrogenous diets was formulated to contain different levels of cottonseed/soybean meal (1 : 1) to replace fish meal (FM) with iron and phosphorus supplementations. The five experimental diets were as follows: diet 1 (control), 0%CS; diet 2, 20%CS; diet 3, 30%CS; diet 4, 30%CS + Fe&P; and diet 5, 40%CS + Fe&P. After 26 weeks of feeding trial, no significant differences were observed in weight gain, feed utilization and survival among all the treatments. The total gossypol accumulation in liver of fish fed diets supplemented with iron was significantly lower than that of fish fed diets without supplementation of iron. The results indicate that the addition of iron in diets could prevent the absorption of free gossypol. The findings in this study suggest that dietary supplements of iron and phosphorus could increase the inclusion of cottonseed and soybean meal for FM replacement in diets for marine fish species.     

Prevalence and different characteristics of two serotypes of Streptococcus parauberis isolated from the farmed olive flounder, Paralichthys olivaceus (Temminck and Schlegel), in Korea

The prevalence of two serotypes of Streptococcus parauberis isolated from the olive flounder, Paralichthys olivaceus, was evaluated in a total of 29 isolates between 2003 and 2010 in Korea. Streptococcus parauberis isolates were divided into two serologically distinct types (serotype 1 and serotype 2), except for one strain (S1091), using an agglutination assay with rabbit antiserum, and serotype 1 was identified as the dominant type (24 of 29 isolates) in this study. To identify the characteristics of the two serotypes of S. parauberis, we conducted a biochemical test using the API 20 Strep kit, a transmission electron microscopy (TEM) assay, sequence analysis of 16S‐23S rRNA intergenic spacer region (ISR) and a pathogenicity test. In TEM, both serotypes possessed polysaccharide capsule layers around the cell surface when bacterial cells were treated with a homologous serotype of rabbit antiserum. However, we were unable to discriminate serotype‐specific biochemical characteristics and genetic characteristics of 16S‐23S rRNA ISR between the two serotypes. In the pathogenicity test, the serotype 1 strains induced significantly higher mortality than the serotype 2 strains in olive flounder when experimentally inoculated via the intraperitoneal route.     

Detection of antigenic proteins expressed by lymphocystis virus as vaccine candidates in olive flounder, Paralichthys olivaceus (Temminck & Schlegel)

Although the major capsid proteins (MCPs) of lymphocystis disease virus (LCDV) have been characterized, little is known about the host-derived immune response to MCPs and other LCDV antigenic proteins. To identify antigenic proteins of LCDV that could be used as vaccine candidates in olive flounder, Paralichthys olivaceus, we analysed the viral proteins responsible for its virulence by applying immuno-proteomics. LCDV proteins were separated by one-dimensional gel electrophoresis, transferred to polyvinylidene difluoride membrane, and probed with homogeneous P. olivaceus antisera elicited by LCDV natural infection and vaccination with formalin-killed LCDV. Four immune-reactive proteins were obtained at 68-, 51-, 41- and 21 kDa using antisera collected from natural infection while two proteins at 51- and 21 kDa exhibited response to antisera from vaccinated fish, indicating that the latter two proteins have vaccine potential. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and nanoelectrospray MS/MS, the 51 and 21 kDa proteins were identified as MCP and an unknown protein, respectively.     

利凉处理对大棚环境、秋番茄生长与结果及其茎基腐病的影响

为验证利凉的应用效果,采用大田试验方法,研究利凉处理下大棚环境、秋番茄生长、结果和茎基腐病的状况。结果表明:在晴天和阴天条件下,利凉处理棚膜均起到降低大棚内的地温、气温和光照强度的作用,同时可提高大棚内的空气相对湿度,为秋番茄生长提供良好的环境条件。与对照大棚相比,利凉处理大棚提高了秋番茄植株的生长速率,可使秋番茄第一花序坐果率提高18.51%,第一层果实产量提高82.66%,同时可降低大棚秋番茄茎基腐病的发病率达15.28%。综上,利凉可以在大棚秋番茄生产上应用。     

The source of autumn and spring downstream migrating juvenile Atlantic salmon in a small lowland river

Over 3 years, 32,444 age‐0 group Atlantic salmon (Salmo salar) parr tagged with passive integrated transponder tags throughout the River Frome catchment were assigned to one of three groups, nonmigrants, autumn migrants and spring smolts, depending on the detection and the timing of detection at downstream tag readers (situated 8.6 km above the tidal limit). We examined the effect of density at the time of tagging (n·m^?2), distance upstream from the tidal limit (km), fish length (mm), Fulton condition index, habitat type (divided into two types, main river and carrier), days after 1 September that each fish was tagged and year (replication) on the proportions of fish in each of the migration groups. Distance upstream from tidal limit was strongly negatively related to the proportion of autumn migrants and positively related to the proportion of spring smolts. Nonmigrants had a lower average body size than migrants, although there were no differences in the sizes of autumn migrants and spring smolts in September prior to migration. Fish density had no effect on migration strategy. A lower proportion of fish migrated as autumn migrants from the smaller carrier habitats than the main river channel. There is some evidence that those parr destined to become autumn migrants underwent a lower mortality rate during September before tagging than those destined to become spring migrants indicating possible physiological or behavioural differences between these two groups of fish at that time. More research into the factors responsible for initiating the autumn migration is required.