浅水芹夏秋轻简栽培技术研究

为探索浅水芹夏秋轻简栽培技术,研究了不同稻草覆盖量和覆盖方式对水芹生长的影响。试验结果表明,浅水芹夏秋栽培宜采用免催芽直接排种;覆盖方式以遮阳网大棚覆盖为最佳,其次为稻草覆盖,最适宜的稻草覆盖量为200~250 kg/667 m2。     

Development of a multiplex PCR assay to detect Edwardsiella tarda,Streptococcus parauberis,and Streptococcus iniae in olive flounder (Paralichthys olivaceus)

A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore, this technique was found to have high specificity when tested with related bacterial species. This method represents a cheaper, faster, and reliable alternative for identifying major bacterial pathogens in olive flounder, the most important farmed fish in Korea.     

树轮宽度记录的1861年以来松潘地区秋雨指数变化特征及其形成机理

为了分析气候变暖背景下松潘地区秋雨的时空变化特征及其形成机理,利用采自四川省松潘县二道海的冷杉树轮样本建立了树轮宽度标准年表,并重建了松潘地区过去150 a秋雨指数,回归方程的误差缩减值(RE=0.19)、乘积平均数(t=3.46)和F检验值(16.4)都达到了0.01的显著性水平,重建方程稳定可靠。研究结果表明:(1)过去150 a松潘秋雨没有明显的线性变化趋势,但是年代际波动显著,先后出现了3个偏强时段和4个偏弱时段。(2)从秋雨指数突变检验来看,松潘秋雨可以分为3个时期,1861—1910年、1961—2010年为明显波动期,1911—1960年为相对平稳期。(3)不同时期松潘秋雨的形成机理存在显著差异,其中1861—1910年松潘秋雨的强弱主要由北方冷空气的强弱主导,1911—1960年则主要受夏季风系统控制,1961—2010年松潘秋雨受冷暖空气同步异常的影响。总体来说,1861—2010年松潘秋雨主要呈现出年代际波动的变化特征,这种年代际差异主要是由于北方冷空气和夏季风系统对松潘秋雨影响程度的差异造成的。     

Removal of Dispersed Dyes from Aqueous Solution Using Activated Carbon Prepared from Olive Stones

In order to valorize olive stones and to show its potential use in the sorption of two dispersed dyes, it was transformed in activate carbon and characterized. The effects of different system variables： pH, agitation speed, temperature and initial dye concentration were studied in the batch tests. The adsorption capacity of activated carbon for the dyes removal was found to be affected by the solution＇s pH. Acidic pH was found the favour disperse dyes removal. Over 95% removal was achieved for both the dyes at pH 3. The equilibrium time for both dyes was 30 min. Both Langmuir and Freundlich isotherms could be used to describe the adsorption of the dyes. Freundlich adsorption model succeeded in fitting the adsorption isotherms of dyes on olive stones activated carbon in single-solute systems, and prediction of the competitive adsorption behavior of dyes with the Freundlich-based Sheindorf-Rebuhn-Sheintuch （SRS） model gave acceptable results.     

南京地区西瓜秋延迟栽培技术研究

为延长西瓜供应时间,实现反季节上市,提高大棚利用率,研究了南京地区西瓜秋延迟栽培技术。结果表明:‘早佳8424’和‘小兰’为最适宜南京地区秋延迟栽培的中型和小型西瓜品种;采用地爬式栽培,西瓜单果重,商品性好,投资较小;采用吊蔓栽培,栽培密度大,西瓜亩产高;种苗8月上旬定植,生长期避开高温,利于高产增收。     

Prevalence and different characteristics of two serotypes of Streptococcus parauberis isolated from the farmed olive flounder, Paralichthys olivaceus (Temminck and Schlegel), in Korea

The prevalence of two serotypes of Streptococcus parauberis isolated from the olive flounder, Paralichthys olivaceus, was evaluated in a total of 29 isolates between 2003 and 2010 in Korea. Streptococcus parauberis isolates were divided into two serologically distinct types (serotype 1 and serotype 2), except for one strain (S1091), using an agglutination assay with rabbit antiserum, and serotype 1 was identified as the dominant type (24 of 29 isolates) in this study. To identify the characteristics of the two serotypes of S. parauberis, we conducted a biochemical test using the API 20 Strep kit, a transmission electron microscopy (TEM) assay, sequence analysis of 16S‐23S rRNA intergenic spacer region (ISR) and a pathogenicity test. In TEM, both serotypes possessed polysaccharide capsule layers around the cell surface when bacterial cells were treated with a homologous serotype of rabbit antiserum. However, we were unable to discriminate serotype‐specific biochemical characteristics and genetic characteristics of 16S‐23S rRNA ISR between the two serotypes. In the pathogenicity test, the serotype 1 strains induced significantly higher mortality than the serotype 2 strains in olive flounder when experimentally inoculated via the intraperitoneal route.     

Detection of antigenic proteins expressed by lymphocystis virus as vaccine candidates in olive flounder, Paralichthys olivaceus (Temminck & Schlegel)

Although the major capsid proteins (MCPs) of lymphocystis disease virus (LCDV) have been characterized, little is known about the host-derived immune response to MCPs and other LCDV antigenic proteins. To identify antigenic proteins of LCDV that could be used as vaccine candidates in olive flounder, Paralichthys olivaceus, we analysed the viral proteins responsible for its virulence by applying immuno-proteomics. LCDV proteins were separated by one-dimensional gel electrophoresis, transferred to polyvinylidene difluoride membrane, and probed with homogeneous P. olivaceus antisera elicited by LCDV natural infection and vaccination with formalin-killed LCDV. Four immune-reactive proteins were obtained at 68-, 51-, 41- and 21 kDa using antisera collected from natural infection while two proteins at 51- and 21 kDa exhibited response to antisera from vaccinated fish, indicating that the latter two proteins have vaccine potential. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and nanoelectrospray MS/MS, the 51 and 21 kDa proteins were identified as MCP and an unknown protein, respectively.     

浅谈旌德秋冬季农业开发

提出了旌德秋冬季农业开发的建议：提高认识,合理布局,扩大规模,科技推动,产业化经营,推动旌德生态农业发展。     

天津滨海树木春植秋植效果分析

通过对天津滨海地区园林工程实践中数据的调查,比较了苗木春植与秋植的效果,明确了苗木秋植在园林工程建设中的重要作用,分析阐述了形成实际工作中秋植苗木成活率高于春植苗木成活率的原因,并提出了春植与秋植相结合的建议。     

利用模糊数学方法预测秋白菜适宜收获期

依据秋白菜受冻的日最低气温指标,利用本溪市1957～2008年逐日最低气温资料,应用模糊数学隶属度函数预测日最低≤-7℃的初日趋势,建立多元回归模型预测日最低气温≤-7℃初日,为指导秋白菜适时收获、合理安排上期提供科学的气象依据。