中国南海周边犬类贾第虫感染情况调查与基因序列分型

贾第虫是一种人兽共患肠道寄生原虫,在全世界范围内传播,其感染率与地区和环境有非常大的关系。为了解中国南海周边国家和地区犬类贾第虫感染情况,判断海洋隔离是否是影响贾第虫传播的重要因素,自2018年6月至2019年7月,从中国广州、越南胡志明市、马来西亚吉隆坡、新加坡和菲律宾马尼拉分别采集犬类粪便样本616份。提取每个地区犬类粪便样本的DNA,以贾第虫磷酸丙糖异构酶(triose phosphate isomerase)基因TPI和BG(genotyping at the beta giardin)基因制作巢式PCR引物。提取的犬粪基因组DNA为模板进行巢式PCR扩增,确定其阳性感染率,PCR阳性产物经测序并将结果与GenBank中的贾第虫序列比对,判断其分型。结果发现,616份样品中,有48份样品检验结果为贾第虫感染阳性,阳性率为7.79%(48/616)。不同地区感染率分别是:中国广州4.81%、越南胡志明市6.90%、马来西亚吉隆坡9.09%、新加坡2.86%和菲律宾马尼拉11.52%。其中48份样品在TPI和BG位点分别检测到条带,阳性率分别为7.63%和5.19%。阳性样品再经过...     

常态下景天科室内观赏植物释放负离子的研究

目的为景天科拟石莲花属植物规模化繁殖和生产提供依据。方法以景天科拟石莲花属植物雪莲种子、红边月影、女雏、花乃井和小蓝衣叶片以及3种播种基质(V_{泥炭土422#}∶V_珍珠岩=3∶2、V_{泥炭土680#}∶V_珍珠岩=3∶2和V_{本地红壤土}∶V_珍珠岩=3∶2)为试材,测定了3种基质的理化性质及其对红边月影、女雏、花乃井和小蓝衣叶插生根率、新芽直径以及对雪莲种子发芽率的影响。结果3种基质的容重、总孔隙度、通气孔隙度、持水孔隙度、有机质和有效氮磷钾元素均存在差异,pH值和EC值均符合拟石莲花属植物的生长要求。3种基质对拟石莲花属植物叶插生根率影响不显著(P>0.05),对新芽直径和种子发芽率影响显著(P<0.05),拟石莲花属植物物种间的叶插生根率和新芽直径存在显著(P<0.05)或极显著(P<0.01)差异,拟石莲花属植物物种和基质间存在互作效应。基质理化性质中,对拟石莲花属植物发芽率和生根率均为显著影响的共性因素为基质中有机质含量、氮元素含量和持水孔隙度,而对生长速度有极显著影响的因素为通气孔隙度。结论泥炭和珍珠岩复配较适宜作为拟石莲花属植物的叶插和播种繁殖基质,针对不同拟石莲花属植物品种筛选不同性质的泥炭,并且在不同生长期选用不同理化性质的基质,可获得更好的繁育效果。     

种植密度与施氮量对江西紫色土烤烟产量及农艺性状的影响

目的探讨不同种植密度与施氮量对山地烟生长发育及烟叶品质特征的影响,以期为山地烟优质高产高效提供理论依据。方法以K326为试验材料,通过大田试验,采用随机区组排列,设置3个种植密度(13875、15150和16665株/hm²)和2个施氮量(90和105 kg/hm²),研究不同种植密度与施氮量对山地烟农艺性状、产值、产量及烟叶品质的影响。结果相同密度下,施氮量的增加可促进烤烟生长发育,产值、产量也增加,但总糖和还原糖均下降,总氮和烟碱含量上升,感官质量提高。同一施氮量水平下,种植密度增加,烟株长势减缓,烟叶产量和产值增加,但烟叶单株质量持续下降,总糖和还原糖升高,总氮和烟碱下降,感官质量总体呈下降趋势。结论适度提高山地烟种植密度和施氮量可提高烟叶产量和质量,即种植密度15150株/hm²和施氮量105 kg/hm²技术组合较为适合山地烟生产开发,其产量较对照提高12.76%,产值较对照提高8.52%,烟叶化学成分更协调,香气量更高,清香型特征更为彰显,较为满足山地烟优质、适产的生产需求。     

Ecological consequences of variation in standard metabolism and dominance among salmon parr

Abstract – Standard metabolic rate (SMR, closely related to basal and resting metabolism) varies by up to threefold among juvenile Atlantic salmon, Salmo salar L., of similar size at common temperature. Here, consequences of this variation are predicted by combining empirically derived relationships between SMR, specific dynamic action, energy budgets, water velocity, food level in the environment and food availability to fish. The range of velocities across which growth is predicted to occur is inversely related to a fish’s SMR. Growth is positively related to SMR at high but negatively related to it at low‐food levels. The relationship between food level and the range of velocities over which lower SMR fish can grow but higher SMR fish cannot is asymmetrically bi‐phasic and peaked. It is predicted that maternal manipulation of offspring SMR would generate fitness benefits through bet‐hedging against unpredictability in food level and increases in the overall range of velocities that the family of offspring can occupy and thrive in.     

粉条加工中添加石蜡的检测技术研究

【目的】探讨粉条加工过程中非法添加石蜡的测定方法,为食品监管提供保障。【方法】先以石蜡成品摸索石蜡测定的气相色谱质谱条件,再以石蜡成分C18H38和C24H50进行回收率和相对标准偏差试验。【结果】不同预处理方法中,超声萃取处理的C18H38和C24H50在粉条中的萃取回收率最高,分别为93.5%和91.0%;20min超声时间萃取粉条中烷烃成分较为适宜;所建立的粉条中石蜡含量测定方法的相对标准偏差为3.6～4.8,样品回收率均大于90.0%,达到定量检测要求。【结论】所建立的石蜡检测方法操作简单,测定结果准确可靠,可用于粉条中石蜡成分的定性及定量测定。     

速生林木材液化及其产物应用研究

[目的]为速生桉树木材液化及其产物的应用提供参考。[方法]以硫酸为催化剂,苯酚为液化剂,对速生人工林桉树木材进行液化,并取液化产物与甲醛进行树脂化反应制备液化木基酚醛树脂LWPF,研究液比对液化反应效率、液化产物重均分子量及LWPF树脂胶合性能的影响。[结果]随着液比的提高,液化产物的残渣率及重均分子量均降低,而LWPF树脂制得的胶合板的木破率提高;当液比提高到一定程度后,液化产物的残渣率和重均分子量及LWPF树脂所制胶合板的木破率变化不明显。[结论]对速生桉树木材的液化工艺及其液化产物的树脂化合成工艺进行了优化。     

湖南省森林公园在全省旅游经济中的地位评析

通过分析近10年间湖南省森林公园旅游收入在全省旅游经济中的地位变化情况 ,发现全省森林公园旅游收入增长势头强劲但不太稳定 ,总体呈波状运行态势 ,在全省旅游经济中所占比重较小但潜力巨大 ,提出了应优化产业结构、推动产业链延伸、健全森林旅游产品体系 ,为全省旅游产业发展培育新的经济增长极.     

应用~(15)N示踪探究楸树无性系对氮素的吸收和分配

为探讨楸树无性系对氮素的吸收、分配及利用特性,以2年生楸树无性系015-1、1-3、7080、1-4和004-1组培苗为试验材料,应用15N示踪技术对楸树无性系进行施肥试验。结果表明,5个楸树无性系氮肥的吸收率、利用率及分配率具有较强的一致性,氮肥利用率介于27.14%~31.24%之间。楸树无性系根和叶的肥料氮比例(Ndff)明显大于茎,楸树无性系根和叶对氮肥的竞争力较强,茎对氮肥的竞争力最弱。015-1茎部氮素分配率及无性系7080根部氮素分配率明显高于其他4个无性系;氮素分配率在各个器官中差异显著,叶片氮素的分配率最高,总体趋势为叶根茎。本研究结果为楸树氮肥的合理施用提供了理论依据。     

Factors Affecting Recipients' Pregnancy,Pregnancy Loss,and Foaling Rates in a Commercial Equine Embryo Transfer Program

During 11 breeding seasons, 351 7- to 10-day-old horse embryos were nonsurgically transferred into recipients that ovulated between 3 and 10 days earlier. Pregnancy rates at 14 and 40 days and foaling rates were 77.8% (273/351), 69.2% (243/351), and 64.4% (226/351), respectively. Pregnancy loss between 14 and 40 days was 11% and between 40 days and delivery was 7%. The transfer of quality grade 3 to 4 embryos resulted in a significantly lower pregnancy rate at 14 days compared with the transfer of grade 1 to 2 embryos (46.2% vs. 79%; P < .05). Eight-day-old embryos resulted in significantly lower pregnancy losses than day 9 or 10 embryos, as occurred for embryos between 400 and 1200 μm compared with embryos <400 μm. Embryos recovered from mares >20 years resulted in a significantly higher pregnancy loss rate than those recovered from younger mares. The same happened for embryos coming from mares affected by reproductive pathologies compared with healthy mares performing sport activity. None of the evaluated parameters influenced recipients' foaling rate significantly.