基于光合有效辐射瞬时值估算日均值的方法

光合有效辐射是太阳辐射的一部分,是植被进行光合作用和陆地生态系统碳循环的核心因素,对估算植被生产力具有重要影响。本文基于SURFRAD多年观测数据,建立了一个线性回归模型,由上午、下午瞬时光合有效辐射值估算日均值。该模型模拟效果较好,单个观测站的均方误差低于9 W·m^-2,判定系数不低于0.96,并适用于不同经纬度、气候条件和海拔高度下的区域,总的均方误差为8.863 1 W·m^-2,判定系数R2为0.977,表明该模型和方法有较好的可行性和可靠性。     

基于NIR定量模型快速预测发酵麸皮中还原糖和可溶性蛋白成分的含量

为建立一种能快速预测发酵麦麸还原性糖和可溶性蛋白含量的定量分析模型,本研究以发酵麦麸为样本,采用3,5-二硝基水杨酸比色法（3,5-dinitrosalicylic acid, DNS）和BCA蛋白浓度测定法分别测定样品的还原性糖和可溶性蛋白含量;利用近红外光谱技术（Near infrared spectrum instrument, NIR）结合偏最小二乘法（Partial least squares regression, PLS）,比较预处理方法、最佳波长及主成分因子的决定系数R²,建立发酵麦麸还原性糖和可溶性蛋白含量的NIR快速检测定量模型。结果表明：1）发酵麦麸还原性糖定量模型的预处理方法使用一阶导数 （First derivative, FD）+二阶导数 （Second derivative, SD）+标准正态变换 （Standard normal variate, SNV）,光谱范围为908~1 670 nm ,主因子数为7时,模型效果最优,其决定系数Rc²为0.904 8,校正均方根误差（Square error corrected, SEC）为1.576 1,相对分析误差（Relative percentage difference, RPD）为3.240 8;外部验证集决定系数Rp²为0.954 9且还原糖活性成分的验证集样本的测定值与NIR光谱预测值的P值为0.959 5>0.05。2）发酵麦麸可溶性蛋白定量模型的预处理方法使用一阶导数 （First derivative, FD）+二阶导数 （Second derivative, SD）+标准正态变换 （Standard normal variate, SNV）,光谱范围为908~1 670 nm ,主因子数为10时,模型效果最优,其决定系数Rc²为0.938 2,校正均方根误差（Square error corrected, SEC）为2.003,相对分析误差（Relative percentage difference, RPD）为4.021 9;外部验证集决定系数Rp²为0.994 4,且可溶性蛋白活性成分的验证集样本测定值与NIR光谱预测值的P值为0.901 9>0.05。综上,建立的NIR光谱定量模型稳定性和准确性较好,且预测准确度良好,可用于快速预测发酵麦麸样品的还原性糖和可溶性蛋白含量。     

Does root-sourced ABA play a role for regulation of stomata under drought in quinoa (Chenopodium quinoa Willd.)

The Andean seed crop quinoa (Chenopodium quinoa Willd.) is traditionally grown under drought and other adverse conditions that constrain crop production in the Andes, and it is regarded as having considerable tolerance to soil drying. The objective of this research was to study how chemical and hydraulic signalling from the root system controlled gas exchange in a drying soil in quinoa. It was observed that during soil drying, relative g_s and photosynthesis A_max (drought stressed/fully watered plants) equalled 1, until the fraction of transpirable soil water (FTSW) decreased to 0.82 ± 0.152 and 0.33 ± 0.061, respectively, at bud formation, indicating that photosynthesis was maintained after stomata closure. The relationship between relative g_s and relative A_max at bud formation was represented by a logarithmic function (r² = 0.79), which resulted in a photosynthetic water use efficiency WUE_Amax_/gs$\text{WU}{\text{E}}_{A_{\text{max}}/g_{\text{s}}}$ of 1 when FTSW > 0.8, and increased by 50% with soil drying to FTSW 0.7–0.4. Mild soil drying slightly increased ABA in the xylem. It is concluded that during soil drying, quinoa plants have a sensitive stomatal closure, by which the plants are able to maintain leaf water potential (ψ_l) and A_max, resulting in an increase of WUE. Root originated ABA plays a role in stomata performance during soil drying. ABA regulation seems to be one of the mechanisms utilised by quinoa when facing drought inducing decrease of turgor of stomata guard cells.     

长白山森林土壤有机碳库大小及周转研究

主要分析不同森林植被下有机碳的分解动态和土壤碳库各组分大小、周转时间。结果表明：土壤样品培养90天，CO2累计释放量表层大致为1723～5065mg／kg、下层大致为178～642mg／kg。分解速率总的趋势是前期快，后期慢，表层明显大于下层。大小顺序为：冷杉林〉针阔混交林和阔叶林〉针叶林。在不同植被下的表层和下层土壤中，活性碳占总有机碳的0．54％～1．67％，0．45％～5．48％．平均驻留时间为11～56天、60～88天；缓效性碳占总有机碳的23．0％～63．3％，33．2％～72．2％，平均驻留时间为4～70年、24～161年；惰效性碳占总有机碳的35．5％～75．5％．26．0％～65．%。表层土壤的总有机碳、活性碳、缓效性碳和惰效性碳含量都明显大于下层。凋落物的化学组成主要决定活性碳库、缓效性碳库含量，土壤的粘粒含量等性质主要决定惰效性碳库含量。     

渔业发展的主体及其行为分析

渔业发展的进程和结果取决于相应的经济主体在发展过程中的行为策略。演化经济学从个体行为的层面很好地解释了推动经济发展的深层因素。论文基于演化经济学的研究视角,针对我国渔业发展的现状,分析了经济主体尚未完全确立、经济主体的行为能力缺乏、所处的市场环境不完善等问题。提出培育具有独立经济行为的主体,使其具有主动适应外部市场环境变化的行为能力,以及完善相应的市场机制的运行,是实现渔业持续发展的关键所在。     

新疆农牧区贫困户参与旅游精准扶贫行为

以新疆农牧区贫困户为研究对象,采用计划行为理论模型以及双槛模型的方法,研究了影响贫困户的参与行为,以期提出减贫路径。结果表明:农牧民是否参与旅游精准扶贫的行为前提为是否有一定的参与意愿。贫困户的个人特征中,家庭创业、人均纯收入、通用电对参与旅游精准扶贫产生了正向的促进作用,与村主干路距离、外出务工人员、思想动力不足导致的贫困对参与旅游精准扶贫产生了负面影响;在行为规范中,指令性规范、示范性规范、合作态度对旅游精准扶贫中的前景看好、知觉行为控制力对农牧民参与旅游精准扶贫产生了正面影响。     

粗腿羊肚菌子囊孢子萌发过程及其细胞核行为

用4μg/mL 4′,6-二脒基-2-苯基吲哚(DAPI)对细胞核染色,采用激光共聚焦显微成像技术观察粗腿羊肚菌(Morchella crassipes)子囊孢子的萌发过程。新鲜的粗腿羊肚菌子囊孢子呈椭圆状,大小13.0~15.3μm×21.5~24.0μm,成熟子囊孢子的细胞核数量为18~32个;CYM液体培养基,23℃培养6h后子囊孢子体积逐渐膨大至初始体积的1.7~2.9倍,泡囊化现象明显;6~12h开始形成芽管,泡囊逐渐消失,出芽点在椭圆形细胞的一端形成或两端先后形成,数量不等的细胞核由子囊孢子内迁至芽管中,形成多核丝状;芽管生长至20~30μm后,在出芽点与孢子连接处形成隔膜,12~24h后,随着芽管的持续生长,形成新的隔膜和菌丝细胞,菌丝细胞多核;芽管及初生菌丝直径6~8μm,约为子囊孢子直径的1/3;24h内没有观察到初生菌丝之间发生细胞融合现象。     

Genetic immunization with the immunodominant antigen P48 of Mycoplasma agalactiae stimulates a mixed adaptive immune response in BALBc mice

A DNA vaccine against contagious agalactia was developed for the first time, encoding the P48 of Mycoplasma agalactiae. Specific immune responses elicited in BALB/c mice were evaluated. Both total IgG and IgG1 were detected in mice vaccinated with pVAX1/P48. Proliferation of mononuclear cells of the spleen, levels of gamma interferon, interleukin-12, and interleukin-2 mRNAs were enhanced in immunized animals. Results indicate that pVAX1/P48 vaccination induced both T_h1 and T_h2 immune responses. Nucleic acid immunization could be a new strategy against M. agalactiae infections and may be potentially used to develop vaccines for other Mycoplasma diseases.