全文获取类型
收费全文 | 8506篇 |
免费 | 404篇 |
国内免费 | 584篇 |
专业分类
林业 | 1077篇 |
农学 | 549篇 |
基础科学 | 182篇 |
816篇 | |
综合类 | 3400篇 |
农作物 | 623篇 |
水产渔业 | 294篇 |
畜牧兽医 | 1501篇 |
园艺 | 591篇 |
植物保护 | 461篇 |
出版年
2024年 | 22篇 |
2023年 | 102篇 |
2022年 | 194篇 |
2021年 | 233篇 |
2020年 | 208篇 |
2019年 | 329篇 |
2018年 | 191篇 |
2017年 | 269篇 |
2016年 | 296篇 |
2015年 | 290篇 |
2014年 | 469篇 |
2013年 | 589篇 |
2012年 | 637篇 |
2011年 | 600篇 |
2010年 | 527篇 |
2009年 | 585篇 |
2008年 | 510篇 |
2007年 | 543篇 |
2006年 | 502篇 |
2005年 | 539篇 |
2004年 | 338篇 |
2003年 | 252篇 |
2002年 | 212篇 |
2001年 | 146篇 |
2000年 | 148篇 |
1999年 | 138篇 |
1998年 | 86篇 |
1997年 | 79篇 |
1996年 | 81篇 |
1995年 | 57篇 |
1994年 | 41篇 |
1993年 | 55篇 |
1992年 | 59篇 |
1991年 | 36篇 |
1990年 | 27篇 |
1989年 | 27篇 |
1988年 | 18篇 |
1987年 | 15篇 |
1986年 | 6篇 |
1985年 | 7篇 |
1984年 | 6篇 |
1983年 | 5篇 |
1981年 | 4篇 |
1980年 | 4篇 |
1979年 | 2篇 |
1977年 | 2篇 |
1974年 | 1篇 |
1956年 | 5篇 |
1955年 | 2篇 |
排序方式: 共有9494条查询结果,搜索用时 0 毫秒
31.
杨衡 《农业图书情报学刊》2005,17(3):154-157
本文从语言特点、编排体例及内容特点出发,对《中图法》(第四版)类目注释进行了系统的认识,并就此提出了自己的一些看法。 相似文献
32.
33.
G. Jager H. Velvis 《European journal of plant pathology / European Foundation for Plant Pathology》1995,101(4):467-478
The position of plants withRhizoctonia solani sclerotia (black scurf) on progeny tubers was mapped for an experimental field at Haren where potatoes were grown continuously and in rotation with other crops for five successive years, and for another field at Borgercompagnie with a 12 frequency of potatoes during three potato crops. Initially, the distribution of plants with black scurf on both fields was rather dense and homogeneous. In the following years the distribution became heterogeneous and patchy. The local decline ofR. solani AG 3 (the common potato pathogen) in Haren was apparently caused by an unknown factor selectively suppressingR. solani AG 3, while simultaneouslyR. solani AG 5 increased in mass. This AG 5 type proved to be an inferior competitor of AG 3 on the potato plant in a laboratory experiment. The specificR. solani antagonistVerticillium biguttatum did not play a role. A similar factor could have reduced the formation of black scurf in the experimental field at Borgercompagnie, whereV. biguttatum was also too infrequent to account for the decline.R. solani AG 5 was not present here and could not indicate the presence of a selective factor against AG 3. 相似文献
34.
通过多点水稻苗田大面积示范试验结果表明,通化市农科所研制的水稻苗田除草剂“封闭1号”具有除草效果好(90~100%)、安全、使用方便、成本低等特点。1988~1991年,累计推广面积(苗田)达15.6万亩,创造经济效益150万元。 相似文献
35.
The effects of root-applied chalcone at 0.15 mmol L−1 on the growth and lignin biosynthesis in maize were investigated. The contents of 4-coumarate: CoA ligase (4CL, EC 6.2.1.12) substrates in maize shoots were increased more rapidly in the samples with chalcone application than in the control and the increase occurred at ≤ 3 h after the application (HAA). The lignin content was reduced by chalcone at ≤ 6 HAA. The shoot growth was suppressed by chalcone at ≤ 9 HAA. Consequently, the results suggest that chalcone suppressed maize growth by inhibiting monolignol biosynthesis. 相似文献
36.
马铃薯渣酶法水解液制备单细胞蛋白饲料的研究 总被引:1,自引:0,他引:1
本文研究了酶法水解马铃薯渣制备膳食纤维后的滤液制备单细胞蛋白的可行性。试验结果表明,单细胞蛋白边糖化边发酵的摇瓶培养的较优工艺条件为:底物浓度为8%(添加8%的麸皮水)、初始pH值为4.5、接种量为15%、葡萄糖淀粉酶加入量为100U/g(原料中淀粉)、青霉素加入量为80U/g(原料)、培养温度为28℃、培养时间为6h、转速为250r/min。在此条件下,干酵母产量最高为19.920g/L,单细胞蛋白中的蛋白质含量达12.27%。 相似文献
37.
38.
陇南山区生态旅游资源评价及开发策略 总被引:12,自引:1,他引:12
陇南山区生态环境脆弱,社会经济落后,同时又是生态旅游资源富集区,正处在西安旅游区、成都旅游区和兰州旅游区的包围之中,区位条件和客源市场较佳,实施生态旅游开发将对发展地方经济和提高游客生态环境意识意义重大。在对其旅游资源、区位条件、客源市场进行定性与定量评价的基础上,提出开发森林生态游、休闲度假游、避暑消夏游、三国怀古游、长征体验游等特色旅游项目,设计出不同尺度、不同主题的若干旅游线路,并针对发展生态旅游所面临的问题提出了相关对策。 相似文献
39.
XU Rui ZHANG Yun ZHANG Mei GE Zhi-ming CHEN Wen-qiang LI Xiu-chang CAI Heng ZHU Hui 《园艺学报》2003,19(5):642-644
AIM:To elucidate the relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats (SHR).METHODS:Intracellular free calcium concentrations were measured by Fura 2 methodology and left ventricular function quantitated by cardiac catheterization in 20 SHR aged 10, 22, and 34 weeks and 20 age-matched Wistar-kyoto (WKY) rats.RESULTS:(1) The systolic blood pressure(SBP), intracellular calcium concentrations and left ventricular mass / body weight index (LVM/BW) were significantly higher in all three age groups of SHR than the corresponding groups of WKY; (2) Compared with age-matched WKY groups, the peak left ventricular pressure descending rate(-dp/dtmax) decreased while left ventricular relaxation time constant (τ)increased significantly in SHR aged 22 and 34 weeks. The peak left ventricular pressure ascending rate(dp/dtmax) and the left ventricular contractility index were significantly increased only in the 34 weeks SHR; (3) Intracellular calcium concentrations showed a positive correlation with LVM/BW,SBP,-dp/dtmax and τ(r=0.47-0.83,P<0.01)and a negative correlation with dp/dtmax and the left ventricular contractility index (r=-0.46,P<0.05 and r=-0.81, P<0.01).CONCLUSION:Intracellular calcium overload is one of the potential mechanisms in the induction of left ventricular hypertrophy as well as of systolic and diastolic dysfunction. 相似文献
40.
AIM:To manufacture recombinant protein of the highly conserved domain in human bone morphogenetic protein-1(BMP-1) using gene engineering methods as antigen for making wide spectrum antibody to BMP-1.METHODS:We analyzed the gene sequences and protein structures of BMP-1 and its related proteins, and chose a highly conserved fragment as target gene. Total RNA was prepared from human osteosarcoma cell line Saos-2, then the target gene was amplified with RT-PCR. The PCR product was cloned into prokaryotic expression vector pMAL c2 to get recombinant vector BMP-1(322-588aa)-pMAL c2. After transforming the recombinant plasmid into DH5-alpha and screening, several prositive clones were got for sequencing. Finally the transformed cells was induced with IPTG to get fusion protein.RESULTS:The BMP-1 gene fragment was successfully cloned into vector pMAL c2, and was able to express efficiently with IPTG inducement. The amount of expressed fusion protein is about 66%-72% in total volume of bacterial proteins.CONCLUSIONS:The recombinant protein contains several key domains(2 CUB domains and 1 EGF domain), which are shared by BMP-1 and its related proteins. Specific wide spectrum antibody to human BMP-1 and its related proteins may be generated with this recombinant protein antigen. 相似文献