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101.
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Yasuhiro Inoue Takayuki Matsuura Tatsuji Ohara Koji Azegami 《Journal of General Plant Pathology》2006,72(2):111-118
The entire genome of bacteriophage OP1, lytic for Xanthomonas oryzae pv. oryzae causing bacterial leaf blight of rice, and the partial genomes of related phages were sequenced and analyzed. The OP1 genome comprises double-stranded, 4785-bp long DNA with 51.1% G + C content. Fifty-nine open reading frames (ORFs) were detected.
ORF25 had similarity with the tail fiber gene of phages, whose product is related to host specificity. The ORF25 regions were
amplified from four host-range mutants (OP1h, OP1hC, OP1h2, and OP1h2C) by polymerase chain reaction, and their deduced amino acid sequences were compared. Three mutants (OP1hC, OP1h2, OP1h2C) had duplications of a small domain in the N-terminal portion, although there were slight differences in the position of the duplicated sequences. One mutant OP1h had substituted amino acids in the duplication region. New mutants isolated in the laboratory (OP1hC and OP1h2C from OP1 and OP1h2) acquired the ability to lyse strain N5874 belonging to phagovar (lysotype) C. However, they rapidly lost this lytic ability
when incubated with other phagovars. This loss was always accompanied by a loss of the characteristic repeats, suggesting
that the host range of OP1-related phages changed mainly through duplication and deletion of a small domain in ORF25.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers AP008979,
AB214312 to AB214316 相似文献
104.
Skeletal muscle is the most abundant tissue and the main component of muscle in animals.The process of skeletal muscle cell's proliferation and differentiation is the basis of muscle development and it's directly affects the meat production performance of domestic animals.It has been found that epigenetic modification plays an important role in regulating the proliferation and differentiation of skeletal muscle cells.In this study,the effects of epigenetics on skeletal muscle in terms of the effects of DNA methylation on the proliferation and differentiation of skeletal muscle cells,the selection and expression of factors regulated by histone acetylation,the regulation of non-coding RNA,and the effects of chromosome remodeling.Research progress in the process of muscle cell proliferation and differentiation,briefly describes the effects of different modification methods and factors on the two processes of skeletal muscle proliferation and differentiation.At the same time,the methods and means used by predecessor in the study of skeletal muscle proliferation and differentiation were reviewed,and then the role of apparent regulatory factors in skeletal muscle growth was analyzed.The purpose was to further explain the important role of epigenetic modification in the proliferation and differentiation of skeletal muscle,enhanced the understanding of the regulation of skeletal muscle proliferation and differentiation,provided a reference path for integration with animal production,and also provided skeletal muscle.Molecular regulation of such as growth and development provided more reference materials. 相似文献
105.
人参总皂苷及其衍生物对小鼠淋巴细胞和NK细胞免疫活性的影响 总被引:6,自引:0,他引:6
为了提高人参总皂苷的生物学活性,对其进行了硫酸化修饰,并探讨总皂苷及其衍生物对小鼠脾T、B淋巴细胞增殖(MTT法)和NK细胞杀伤活性(乳酸脱氢酶释放法)的影响。结果表明,2种衍生物质量浓度在50~500 mg/L时可以促进小鼠脾T、B淋巴细胞的分化增殖和NK细胞对YAC-1的杀伤作用,质量浓度较高时促进作用明显优于总皂苷;衍生物质量浓度达到1 400 mg/L时,仍然可以显著促进T淋巴细胞的增殖。试验结果提示,人参皂苷的这种衍生物有可能成为一种毒副作用较小、免疫活性更强的药物。 相似文献
106.
We improved the overall performance of fast-growing poplar by utilizing a low-cost, effective and simple method. The fast-growing poplar was modified by a vacuum-pressure impregnation method with three... 相似文献
107.
以玉米秸秆(RCS)为原料,经氨水预处理得预处理玉米秸秆(PCS),再经乙酸酐酯化改性制备改性玉米秸秆(ECS)吸油材料,并考察不同酯化条件对产物吸油性能的影响。结果表明:RCS、PCS(预处理6 h)对0~#柴油的吸收倍率分别为1.90和4.43 g/g;以冰乙酸为溶剂,冰乙酸与乙酸酐质量比为1∶1,秸秆与冰乙酸-乙酸酐混合液的质量比为1∶15,催化剂浓硫酸用量为4.5%(以秸秆质量计),反应温度为110℃条件下,酯化反应5 h所得改性产物ECS的吸油率可达9.03 g/g,且ECS疏水性和漂浮性能均得到显著改善。采用XRD、FT-IR、SEM和BET等方法对预处理前后及酯化改性后秸秆进行了分析与表征。结果表明:酯化反应已顺利进行,酯化改性后秸秆呈非晶态,ECS呈均匀的介孔结构,介孔及粗糙表面的出现提高了材料的吸油率和漂浮性能。 相似文献
108.
水载型木材防腐剂中有效成分固着的影响因素 总被引:1,自引:0,他引:1
水载型木材防腐剂在木材防腐市场中占据着主导地位,然而其中的有效成分的抗水流失性差是其存在的主要缺点,这在很大程度上是由于防腐处理材在使用前防腐剂有效成分与木材成分之间的固着反应不充分而造成的,因此提高防腐有效成分在处理材中的固着质量是提高抗流失性的关键.笔者对国内外在水载型防腐剂中有效成分固着方面的研究进行了较为系统、全面的介绍,其中包括木材树种、固着的环境温度与相对湿度、固着时间、木材含水率、防腐剂的保持量、防腐剂成分组成等不同因素的影响,并在此基础上提出了今后的研究方向. 相似文献
109.
软X—射线法测定木材生长轮密度的研究 总被引:5,自引:2,他引:3
本文开发了一种用于木材生长轮材质变异分析的软X-射线测量法,对14种木材进行了测定和分析,获得了满意的测量结果。 相似文献
110.