草莓微繁殖苗移栽后开花结果的研究

以草莓品种丰香、吐德拉、全明星、北辉为试材,研究了移栽时期、生根方式对草莓微繁殖苗移栽后开花结果的影响。草莓微繁殖苗在春季移栽时期对开花结果习性具有重要影响,2月份移栽,4个品种都开花结果,成熟期比露地栽培草莓大约晚20d,全明星、吐德拉和丰香3个品种的产量与普通苗露地栽培的产量相近;3月份移栽,虽然有开花结果,但并无经济学产量;4月份移栽,4个品种均未开花。不同品种的微繁殖苗在开花株率、单株产量等指标上差异显著,其中全明星微繁殖苗的开花结果能力最强,2月份移栽的微繁殖苗的开花株率达89.3%,单株产量达98.2g。在花序数上,培养基中不含植物生长调节剂处理显著高于附加植物生长调节剂的处理。     

Effect of cell transplantation for the treatment of acute myocardial infarction using vascular endothelial growth factor gene transferred neonatal cardiomyocytes

AIM:To observe the secretion of vascular endothelial growth factor (VEGF) when adenovirus induced VEGF165 (AdVEGF165) gene transferred into neonatal cardiomyocytes in vitro,and to investigate the impact on heart function after transplanted the transferred cardiomyocytes into infarct myocardium in rats.METHODS:Neonatal cardiomyocytes were cultured in vitro and labeled with BrdU,then transferred by AdVEGF165.ELISA was applied to assay the expression and secretion of VEGF.Wistar rats,in which left descending branch of coronary artery was ligated,were randomly divided into four groups and transplanted into MI area with transferred cardiomyocytes (group Ⅰ),untransferred cardiomyocytes (group Ⅱ),AdVEGF165 (group Ⅲ) and culture medium (group Ⅳ),respectively.The echocardiograph was applied to evaluate the heart function before and after cell transplantation.Then the rats were executed and the hearts were harvested for histological (hematoxylin-eosin) and immunohistological (anti-BrdU dyeing) examinations.The vessels were also counted in injected area.RESULTS:ELISA indicated that the expression and secretion of VEGF in groupⅠwere higher than those in the rest (P<0.01).Echocardiograph revealed that the improvement of ejection fraction (EF) in groupⅠwas greater than that in other groups (P<0.01).Immunohistological study showed that the implanted cardiomyocytes survived in MI area.More blood vessels in groupⅠthan other groups were observed (P<0.01) by vessel-counting.CONCLUSIONS:AdVEGF165 gene transfer increased the VEGF-production,accelerated neovascularization in MI area and increased the blood flow.The method makes favor for the survival of implanted cells and greatly improves the heart function.     

大鼠糖尿病模型制备与全胰十二指肠移植术研究

用１％的Ａｌｌｏｘａｎ以５０ｍｇ／ｋｇ．Ｗ的剂量经尾静脉快速注射，成功地在健康雄性Ｗｉｓｔａｒ大鼠制成了糖尿病模型，以健康大鼠为供体，成功地进行了模型大嫌十二指肠移植术。为激光等抗移植排斥反应的深入打下了良好的基础。     

Tissue irradiation injury and past-transplantation distributing diversity of Sca-1 positive cells from murine fetal liver

AIM: To study the relationship between tissue irradiation injury and past-transplantation distributing diversity of Sca-1 positive cells from murine fetal liver and their offspring cells in multi-organs after syngeneic sex mismatch hematopoietic remodeling.METHODS: The Sca-1 positive cells from the livers of C57BL/ 6j male mouse fetus aged 14.5 day were identified and separated by quick PCR and magnetic cell sorting (MACS) techniques. In order to reconstruct hematopoiesis of the adult female mice, which were lethally irradiated with 8G of ［Co⁶⁰］, the 2×10⁴ of Sca-1+ cells were transplanted through caudal vein into each of them. After 6 months, these recipient mice were sacrificed, and their kidneys, livers, lungs, stomachs, and small intestines were taken out, fixed and slices were made. Fluorescence in situ hybridization was performed and observed by fluorescent microscope. Images were captured and analyzed through appropriative cool CCD and software. RESULTS: After 2×10⁴ of Sca-1+ cells were transfused, the hematopoietic function in the lethally irradiative female mice was completely restored. The cells containing Y chromosome were observed 6 months latter in multi-organs, including kidney, liver, lung, stomach, and small intestine. The frequency of the cells containing Y chromosome respectively was kidney (1.65±0.18)%, liver (0.90±0.10)%, lung (1.90±0.60)%, stomach (6.10±0.50)%, and small intestine (7.61±2.30)%, presented the trend of small intestine>stomach>lung>kidneys>liver. Combined informational analysis showed that the presenting frequency of the cells containing Y chromosome was consistent with the irradiative sensitivity of the organ. CONCLUSION: These findings suggest that the capability of differentiation of Sca-1 positive cells from murine fetal liver was potentially connected to the extent of damage in these organs when transferred in vivo.     

猪胚胎细胞核移植研究进展

细胞核移植是目前倍受学术界和国际社会关注的焦点问题之一.已经获得牛、羊、猪、兔、鼠等核移植个体,而且成功地得到体细胞核移植绵羊和小鼠.尽管猪胚胎细胞核移植也有报道,但进展缓慢.现对猪细胞核移植的研究现状,以及与之有关的卵母细胞孤雌激活等问题作较系统的综述.     

泊湖优质沉水植物的移植扩繁效果研究

[目的]探讨泊湖沉水植物的移植扩繁效果。[方法]2008～2009年,选择泊湖水深0．5～1．5m的区域作为移植扩繁优质沉水植物示范区,利用播种和网袋垂挂育苗等方法移植扩繁菹草、轮叶黑藻、苦草等优质沉水植物。[结果]当菹草鳞状石芽播种量为15000g/hm^2。时,其扩繁生物量达3935g／m^2。当轮叶黑藻种芽和苦草种子播种量为3750g／hm^2时,移植扩繁区沉水植物群落的总生物量为2573g／m^2,其中轮叶黑藻的平均生物量为1352．5g／m^2,苦草的平均生物量为631．3g／m^2。[结论]选择优质沉水植物菹草、轮叶黑藻和苦草在泊湖适宜水深移植扩繁效果显著。     

铁坚油杉野生大苗移植驯化栽培简报

铁坚油杉是我国的特有树种,作者于2003年12月修文县小箐乡移栽8株到扎佐林场进行栽培驯化,通过野生铁坚油杉大树进行移栽试验,经过4年生长,带土移栽成活6株,初步证明对其有限的资源进行引种驯化、异地保护是可行的。     

晋中麦后不同苗龄移栽对玉米生长及产量的影响

在山西晋中冬麦区,利用秸秆育苗钵进行不同苗龄麦后移栽玉米试验,对夏玉米的生长、产量进行了研究。结果表明,在充分利用现有的光热资源条件下,利用秸秆育苗钵育苗,在幼苗二叶一心和三叶一心的苗龄下移栽玉米,可以实现早栽培、延长玉米有效生育期,使中晚熟玉米可以正常成熟,从而提高当地玉米产量。     

Effect of VEGF on morphology and function of rat ovarian tissues after autologous transplantation

AIM: To evaluate the effect of vascular endothelial growth factor (VEGF) on the ovarian tissues during transplantation. METHODS: Twenty regularly cycling rats were randomly divided into 2 groups: the rats in group A received fresh autologous ovarian transplantation without VEGF administration (n=10); the rats in group B received fresh autologous ovarian transplantation with VEGF administration (n=10). Four weeks after transplantation, the ovarian tissues and sera of the rats were collected for histological examination and determination of the hormone levels. RESULTS: The serum concentration of estradiol in group B was significantly higher than that in group A (P<0.05). Four weeks after transplantation, we found that the ovarian tissues were significantly developed, and the average size of the ovaries in group B was significantly larger than that in group A (P<0.05). CONCLUSION: The ovarian tissues with VEGF administration develop better than those without VEGF administration. VEGF can increase the graft vascularity and the percentage of surviving tissues.