欧李试管苗生根与移栽技术的研究

对欧李试管苗进行生根与移栽试验结果表明，欧李试管苗生根适宜的培养基配方为：MS＋IAA3．0mg／L＋PP3330．05mg／L＋蔗糖20g／L＋琼脂7g／L，培养23d，生根率达80％以上。移栽时用灭过菌而且通气透水性强的栽培基质为好，移栽成活率达80％以上。     

A seeding experiment of juvenile great scallops (Pecten maximus (L.)) off the Island of Jersey.

In 1995, landings of Great scallops, Pecten maximus(L.) increased dramatically in Jersey from around one tonne in the previousyear, to 66 tonnes and this continues to rise. This was caused by theintroduction of scallop diving permits and diversification of the fishingfleet.Due to this increase in effort it was decided that the feasibility of ranchingone-year-old juvenile scallops should be investigated. 100,000 scallops werepurchased from Ireland and seeded in specifically selected coastal sites. Thescallops grew from 22.8 mm shell length and 1.17 grams to 57.6mm and 23.2 grams in the first six months after seeding, and to93.3 mm and 88.9 grams during the subsequent 12 months. Growthrateslowed considerably during winter months. Given these growth rates the scallopswill reach market size in three years from settlement, less than the 4, 5 and 6years taken in Guernsey, the Eastern Channel and the offshore Irish Searespectively. Although growth rates are not unique and are comparable withotherinshore sites in the UK, they are significant for scallop farming in Jerseywaters. Mortality following re-seeding and predation rates by crab and starfishappears to be lower than reported by other workers. However this has not yetbeenquantified.     

大剂量激光照射、免疫抑制剂及二者配合应用对大鼠全胰十二指肠移植的影响

用健康雄性Ｗｉｓｔａｒ大鼠制备糖尿病模型，以成功的模型大鼠作为受体，健康雄性大鼠为供体，行全胰十二指肠移植术。用移植成功大鼠进行大剂量激光照射、免疫抑制剂及二者配合抗移植排斥反应的实验，于术后隔天监测血糖、尿糖的变化，于术后７天及出现血糖、尿糖持续升高时采取移植胰脏，进行病理组织学观察，以了解排斥反应的发生与程度。结果表明，日剂量为３９．７２４５Ｊ／ｃｍ￣２的激光照射，可推迟排斥反应的发生时间、降低排斥反应发生程度及延长大鼠全胰十二指肠移植物的存活时间。３９．７２４５Ｊ／ｃｍ￣２的激光照射与８～５～３ｍｇ／ｋｇ／ｄａｙ的硫唑嘌呤（Ａｚａ）配合，上述作用更为显著，超过各单一使用的效果，且与环孢霉素Ａ（ＣｓＡ）的作用效果接近。并证实２５～２０～１５ｍｇ／ｋｇ／ｄａｙ的ＣｓＡ及１０～８～５ｍｇ／ｋｇ／ｄａｙ的Ａｚａ抗全胰十二指肠移植抗排斥反应效果明显。     

Study on histocompatibility of pinealocyte microencapsules

AIM: To explore the histocompatibility of pinealocyte microencapsules in vivo. METHODS: The pineal glands of neonatal rats were removed under operating microscope and pinealocytes were isolated through collagenase and trypsin digestion. Pinealocytes were cultured for one week in vitro and collected immediately after digesting was encapsulated in APA microencapsules. The cells and empty capsules were transplanted into abdominal cavity or intermuscular space respectively and retrieved at the 15th or 30th day after operation. Morphological observation, HE staining, cell counting, and HPLC technique were used to analyze the shape, proliferation and function, the degree of inflammation fibrosis of retrieved microencapsules. RESULTS: The retrieve rate of cell capsule from abdominal cavity was about 85%. The retrieved capsules had integrated profile mostly although some were damaged. The amount of macrophages attached to capsule wall and the thickness of wall increased gradually following the period of transplantation. However, the retrieve rate, wall thickness had no difference between retrieved cell and empty capsules at the same time. Secretion ability of pinealocytes in capsule retrieved at 15th day after operation decreased rapidly and those retrieved at 30th day after operation lossed secretion function. CONCLUSION: APA microencapsules had histocompatibility relatively in vivo and protected pinealocytes in capsule from immunologic rejection of the host. The survival time was about 20 days. During this period cells in capsule maintained activity and MT secretion ability.     

黄脉爵床快速繁殖技术研究

黄脉爵床组织培养快速繁殖，基本培养基以MS和1/2MS为好，植物激素为BA和NAA，BA质量浓度为1.0mg/L左右，NAA质量浓度为0.1mg/L左右。在扩大繁殖过程中将单芽和分丛生芽法改用微型短枝扦插法，可加快繁殖和提高移栽成活率。     

猕猴桃试管苗移栽条件研究

在普通塑料大棚内，对影响猕猴桃试管苗移栽成活的因素进行研究，结果表明，空气湿度、温度、移栽季节是影响试管苗成活的关键因子，同时充分的炼苗也是获得高成活率的必要条件。在移栽1－15d内，空气湿度保持在90％以上，能获得较高的成活率。适宜的移栽温度为8－22℃较适宜。移栽基质为沙：土：粪＝2：1：1的复合土；除夏季高温时期成活率低外，其余季节均可进行移栽，而以冬季移栽成活率最高。     

蛤蟆通水库东方欧鳊移殖试验

蛤蟆通水库东方欧鳊移殖试验ＴＥＳＴＯＮＴＨＥＴＲＡＮＳＰＬＡＮＴＡＴＩＯＮＯＦＡＢＲＡＭＩＳＢＲＡＭＡＯＲＩＥＮＴＡＬＩＳＴＯＨＡＭＡＴＯＮＧＲＥＳＥＲＶＯＩＲ夏重志姜作发董崇智赵春刚（黑龙江水产研究所，哈尔滨１５００７０）ＸｉａＺｈｏｎｇｚｈｉ，Ｊ...     

Decellularized porcine corneal posterior lamellae as carrier matrix for cultivating human umbilical vein endothelial cells in vitro

AIM：To investigate the feasibility of corneal posterior lamellar reconstruction with human umbilical vein endothelial cells (HUVECs) and porcine cornea acellular matrix in vitro, and to observe the physiological function of the transplantation in vivo. METHODS：HUVECs were isolated, cultured, and labeled with fluorescent dye CM-DiI. Porcine corneas were treated with 100% glycerinum, cut to a thinner structure step by step, and dried on the super-clean bench. Transmission electron microscope were used to observe the histological changes of the porcine cornea acellular matrix. Labeled HUVECs were seeded onto the porcine cornea acellular matrix, and examined by scanning electron microscopy. When the HUVECs and Descemets membrane fusion formed a monolayer, the corneal transplantation in rabbits was performed. Twenty-four New Zealand white rabbits were randomly divided into experimental group and control group (n=12 each), and their left eyes served as recipients. RESULTS：Cultured HUVECs exhibited polygonal shape. More than 90% HUVECs were labeled with CM-DiI and the cell membrane was positive with red fluorescence, which was detectable at least up to 3 generations. The histological examination indicated that porcine cornea cells were clearly extracted, and the collagen fibers were well arranged. A continuous monolayer of HUVECs on the porcine cornea acellular matrix was observed under scanning electron microscopy. The reconstructed corneal posterior lamellae were similar to the normal cornea. The observation of transplantation showed that the cornea in experimental group was substantially transparent. However, that in control group was oedematous and adiaphanous. CONCLUSION：Corneal posterior lamellae can be reconstructed in vitro by cultivating HUVECs on porcine cornea acellular matrix. After xenogeneic transplantation, the graft survives in vivo and expresses normal corneal endothelial cell biological functions. Deep lamellar corneal endothelial transplantation is an effective keratoplasty.     

不同移植时间对油茶两段法育苗苗木的影响

[目的]研究不同移植时间对油茶（Camellia oleifera Abel.）两段法育苗苗木的影响,以确定准确的移苗时间。[方法]采用完全随机区组设计,多次重复,进行育苗对比试验,测定苗木移栽成活率和苗高。[结果]苗木移栽成活率以10月12日移植最高达99%,10月31日移植次之,达97%;9月21日最低,仅为80%。苗木高度以10月12日移植最好,平均苗高达12.6 cm;10月31日移植次之,达10.9cm;9月21日移植最差,只有7.8 cm。[结论]不论是苗木移栽成活率还是苗高生长量及质量,油茶幼苗在10月中下旬移植最好。     

不同栽培基质对葛萝槭生长发育的影响

以平均苗高7.4cm、平均地径1.82 cm、无病虫害的一年生葛萝槭扦插苗为试验材料,采用农林废弃物醋渣、松针、菇渣主要基础性基质,分别与粉煤灰、树皮粉、锯末、羊粪以不同的体积比混合而成,对不同育苗基质上生长的葛萝槭的苗高、地径、叶片数、冠幅、成活株数进行调查。结果表明:处理9的成活率最高达到了92.78%,表现最好。处理9、处理1、处理3、处理8、处理10可推广使用;不同基质对苗高、叶片数的相对生长量存在显著性差异(α=0.05),对地径、冠幅的相对生长量不存在显著性差异(α=0.05);不同基质处理的葛萝槭的年生长规律一致即3月下旬到4月上旬进入树液流动期,4月中旬到6月上旬为植株的生长初期,6月中旬到8月为速生期,9月进入苗木的硬化期,且在7月中旬出现了生长暂缓期现象。