Transplantation of exogenous adipose tissue derived mesenchymal stem cells for treatment of acute myocardial infarction in rat

AIM: To establish a method of isolating,culturing the adipose tissue-derived mesenchymal stem cells in vitro and to investigate the possibility of exogenous transplanting the adipose tissue derived mesenchymal stem cells for treatment of rat acute myocardial infarction.METHODS: 18 male rats were separated randomly into 3 groups: sham surgery group (control,n=6),acute myocardial infarction control group (AMI,n=6) and myocardial infarction plus cell transplantation group (AMI+cell,n=6).The infarcted hearts were made by occlusion of left coronary artery.The mesenchymal stem cells were isolated from the rats’ peritoneum by using digestion methods and reproduced in vitro,then the cells were labeled with BrdU and implanted into the infarcted heart of the rats.Heart functions were measured 4 weeks after implantation.The hearts were also harvested for pathological and histoimmunochemical observations to determine the survival and location of the implanted cells.RESULTS: Plenty of mesenchymal stem cells were obtained from the adipose tissue of rats’ peritoneum.Compared with the AMI group,the left ventricular systolic pressure in the cell therapy group was increased significantly (P<0.01),the left ventricular end-diastolic pressure was decreased (P<0.01),and the ratio of the left ventricular pressure rise and decay (±dp/dt) was decreased (P<0.05).The number of blood vessels was increased at the boundary of infarction site by pathological observation.The labeled cells were founded in the infarcted myocardium and the blood vessel wall.CONCLUSION: The adipose tissue is a new optional stem cell source.The methods of exogenous transplantation of adipose tissue derived mesenchymal stem cells for treatment of AMI is effective and feasible.     

Depressant effect and mechanism of atorvastatin on the chronic rejection of aortic allograft in rats

AIM: To investigate the depressant effect and mechanism of atorvastatin on the chronic rejection of aortic allograft in rats.METHODS: The models of abdominal aorta transplantation were made with micro-surgery in rats.The recipients were divided into three groups: allograft control group,atorvastatin-treated group and isograft control group.Vascular intimal thickness in all of the groups was observed by histological examination.The expression of proliferation cell nuclear antigenl(PCNA) and α-smooth muscle actin(α-SMA) were determined by immunohistochemistry.The content of nitric oxide was measured by nitrate reductase chromatometry.RESULTS: The vascular intimal thickness in atorvastatin-treated group (11.60%±2.40%) was lower than that in allograft control group (34.60%±6.40%,P<0.05) and higher than that in isograft control group (1.15%±0.65%,P<0.05).The expression level of PCNA was decreased in atorvastatin-treated group (4.80%±0.80%) than that in allograft control group (18.40%±1.80%,P<0.05) and higher than that in isograft group (1.20%±0.40%,P<0.05).CONCLUSION: The expression of PCNA in the transplanted aorta is suppressed by atorvastatin,which results in relief of chronic rejection of aortic allograft.     

南蛇藤试管苗生根培养基及移栽基质的筛选

对南蛇藤无根试管苗在不同生根培养基、瓶内生根苗在不同基质中的生长情况进行的研究结果表明,生根效果较好的培养基为1/2MS ABT0.8mg/L(单位下同) 1.5%蔗糖和1/2MS NAA1.0 1.5%蔗糖,生根率分别为59.4%和58.4%,蛭石、珍珠岩=1∶2为最佳移栽基质,移栽成活率为96.8%。     

苹果优良矮砧S_(18)、s_(20)微茎尖脱毒组织培养技术研究

通过对武乡海棠(Malus honanensis Rehd.)优良矮砧S_(18)、S_(20)两个单系的0.2mm微茎尖脱毒组织培养技术研究,解决了微茎尖切割、接种、分化及生根移栽等一系列技术难题,获得了S_(20)、S_(18)一批脱毒试管苗及移栽壮苗.分化数分别达2.0和3.6,生根率达76.1%和90.0%,平均根长2cm以上,平均根数5条,移栽成活率达78%.