Lignan production inDaphne odora cell cultures

Lignan production in callus and cell suspension cultures ofDaphne odora is reported for the first time. The cell suspension culture produced pinoresinol, lariciresinol, secoisolariciresinol, matairesinol, and wikstromol. The production of matairesinol in the cell suspension culture was much higher than that inDaphne odora stem tissues.Part of this report was presented at the 51th annual meeting of the Japan Wood Research Society, Tokyo, April 2001     

豆科树种无性快速繁殖技术研究与进展

豆科树种目前已广泛应用于荒山绿化、饲用及观赏。文中从扦插(嫩枝扦插、硬枝扦插、根插)，组织培养等方面综述了豆科树种无性快速繁殖技术的研究和进展。指出目前豆科树种嫩枝扦插技术研究还局限在对某一树种繁殖途径的探索阶段；硬枝扦插技术研究较为系统和深入，对不同树种扦插生根的影响因素有了比较深入的了解，并因此建立起较为成熟的技术体系；根插方法简单，成活率高，但是研究普遍较少；豆科树种组织培养虽较为困难，但某些树种也已经建立起了较为完善的再生体系。此外，还对豆科树种快速繁殖的生理基础研究进行了讨论，建议加大对豆科树种快速繁殖技术和生理机制的研究力度，为解决优良种质资源快速扩繁奠定基础。     

心叶球兰组织培养与快速繁殖试验

以心叶球兰叶片为外植体,愈伤组织诱导和继代增殖培养基MS+6-BA2.0mg.L-1+NAA0.5mg.L-1+2,4-D1.0mg.L-1+GA1.0mg.L-1+白糖3%,诱导的愈伤组织多,生长旺盛,增殖倍数为2～3倍;丛芽诱导培养基MS+KT0.5mg.L-1+NAA0.5mg.L-1+GA1.0mg.L-1+白糖3%,芽的诱导率为79.3%;在丛芽增殖及复壮培养基1/2MS+IBA1.0mg.L-1+白糖2%上,有20%～30%玻璃化丛芽转为绿色苗,玻璃化单芽经复壮培养长成绿色苗;诱导生根培养基为1/2MS+IBA0.5mg.L-1+白糖2%,玻璃化生根率为56.4%,淡绿色苗生根率达91.1%。瓶苗移栽基质泥炭土∶珍珠岩∶甘蔗渣(1∶1∶1)的成活率最高,达91.4%。     

Tissue culture micropropagation of Douglas-fir

A procedure was developed for plantlet production from embryos of Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco]. The Medium for Conifer Morphogenesis, used at full strength, and supplemented with 10 M benzyladenine for 17 days produced an average of 6.8 shoots on more than 90% of the embryos. The percentage of shoot-forming embryos as well as the average number of shoots per embryo varied significantly among eight seedlots. For secondary multiplication, 89% of the adventitious shoots produced axillary buds on MCM with 5 M benzyladenine. However, 0.5 M BA was more suitable for the elongation of axillaries. Rooting ranged from 0–87% depending upon the treatment. The highest percentage was obtained with a 7-week incubation in peat:perlite containing 1/5-strength medium, 1% sucrose and 2.7 M naphthaleneacetic acid, followed by 5 weeks on peat:perlite with 1/5-strength major and minor salts, full iron and organics, 1% sucrose and 0.1% charcoal.     

台湾金线莲增殖培养试验

以台湾金线莲茎尖为外植体诱导的腋芽作为中间繁殖体材料,开展基本培养基和植物生长调节剂对增殖培养的影响,结果表明,基本培养基、6-BA和NAA及其水平对台湾金线莲茎尖的增殖均有显著或极显著影响,最佳增殖培养基的配方是MS+6-BA 3.0 mg·L-1+NAA 0.1 mg·L-1,其繁殖系数达5.9。     

对提高伊春林区森林公园生态旅游功能的探讨

森工老林区的林业发展,已步入以生态建设为主的轨道,在传统林业向现代林业的转变中,发展生态旅游已成新宠。伊春林区继五营国家森林公园后又陆续建立了一批森林公园或类似于此的诸多山庄、渡假村等。由此,就伊春林区森林公园的发展现状及生态旅游功能的发挥提出了建设性意见。     

红叶石楠扦插育苗技术

在查阅有关文献和总结育苗经验的基础上,从苗圃地的选择与规划、采穗圃的营建及管理、扦插技术、苗木移栽及病虫害防治等方面总结了红叶石楠无性扦插育苗技术,为红叶石楠苗木的规模化生产提供参考。     

The effect of short day treatments on containerized Douglas-fir morphology,physiology and phenology

The effect of short day treatments (blackout) on Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) container seedlings at the time of lift and following cold storage was investigated. Variables measured included height, root collar diameter (RCD), root growth capacity (RGC), photosynthetic efficiency after –18 °C freezing (PEF), and days to terminal bud break (DBB). From one to four blackout dormancy induction treatments were started on three dates (July 12, July 26, and August 10) with 10 or 20 d between multiple blackouts. Increasing the number of blackout treatments resulted in lower RCD, lower DBB in the late winter/early spring, and higher PEF in the early fall. Later blackout start dates decreased PEF in the early fall, and increased overall height and late fall RGC as compared to earlier blackout start dates. Nurseries growing Douglas-fir seedlings from coastal Pacific Northwest provenances should be aware that blackout regimes can decrease RGC in the late fall, and cause quicker dormancy release in the early spring. Coastal Douglas-fir can be lifted and planted in the early fall, when RGC and DBB are relatively high. If planting between February and April is necessary, seedlings given blackout should be cold stored in January to maintain an adequate level of dormancy, RGC and PEF.     

浅议贵州森林旅游的深层次开发

在分析旅游文化与贵州森林旅游深层次开发相互关系的基础上，结合贵州森林旅游业发展的实际情况，阐述了贵州森林旅游深层次开发的必要性和可行性，提出贵州森林旅游深层次开发的设想和保障措施。     

红托竹荪母种分离培养初探

通过不同培养基培养红托竹荪母种，探明红托竹荪母种培养基的最佳营养条件，为红托竹荪生产提供母种制种技术。