全文获取类型
收费全文 | 1865篇 |
免费 | 102篇 |
国内免费 | 65篇 |
专业分类
林业 | 164篇 |
农学 | 93篇 |
基础科学 | 57篇 |
173篇 | |
综合类 | 993篇 |
农作物 | 38篇 |
水产渔业 | 54篇 |
畜牧兽医 | 305篇 |
园艺 | 102篇 |
植物保护 | 53篇 |
出版年
2024年 | 5篇 |
2023年 | 31篇 |
2022年 | 30篇 |
2021年 | 56篇 |
2020年 | 53篇 |
2019年 | 50篇 |
2018年 | 48篇 |
2017年 | 61篇 |
2016年 | 79篇 |
2015年 | 74篇 |
2014年 | 123篇 |
2013年 | 130篇 |
2012年 | 173篇 |
2011年 | 150篇 |
2010年 | 111篇 |
2009年 | 130篇 |
2008年 | 115篇 |
2007年 | 131篇 |
2006年 | 97篇 |
2005年 | 77篇 |
2004年 | 47篇 |
2003年 | 46篇 |
2002年 | 32篇 |
2001年 | 25篇 |
2000年 | 31篇 |
1999年 | 13篇 |
1998年 | 19篇 |
1997年 | 14篇 |
1996年 | 10篇 |
1995年 | 9篇 |
1994年 | 11篇 |
1993年 | 10篇 |
1992年 | 4篇 |
1991年 | 6篇 |
1990年 | 6篇 |
1989年 | 10篇 |
1988年 | 1篇 |
1987年 | 3篇 |
1984年 | 3篇 |
1982年 | 3篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1977年 | 1篇 |
排序方式: 共有2032条查询结果,搜索用时 312 毫秒
981.
982.
Abstract AIMS: To estimate the seroprevalence of antibodies to Erysipelothrix rhusiopathiae in chickens in New Zealand, and to estimate the effect of housing type, geographical location and age on seroprevalence. METHODS: A cross-sectional serological survey of a convenience sample of 545 broiler, breeder, and layer chickens in 55 flocks was conducted in 2010–2011. Birds were aged 5–83 weeks; housing types were free-range, shed, caged, and unknown; and flocks were located in the Auckland, Manawatu, North Canterbury, Otago, Taranaki, Waikato, and Wairarapa regions of New Zealand. An ELISA was used to measure antibodies to E. rhusiopathiae. Samples with an optical density reading ≥1.50 were considered to be positive. Logistic regression analysis was used to model the effect of housing type, geographical location and flock age on the prevalence of samples positive for antibodies to E. rhusiopathiae. RESULTS: The overall prevalence of samples with antibodies to E. rhusiopathiae was 39.8 (95% CI=35.68–44.06)% for the 545 samples, and 46/55 (84%) farms that were tested had at least one positive sample. Mean seroprevalence for types of housing was 44.2 (95% CI=37.79–50.70)% for free-range (n=240 birds), 23.7 (95% CI=17.83–30.38)% for shed (n=190), 73 (95% CI=56–86)% for caged (n=37) and 50 (95% CI=38–62)% for unknown (n=78). The disease was present in all seven geographical locations from which samples were obtained for this study. Seroprevalence increased with increasing age of birds (p<0.001); for birds ≤12 weeks of age it was 2 (95% CI=0.3–8)% (n=91), 13–24 weeks 29.1 (95% CI=23.34–35.46)% (n=230), 25–36 weeks 47 (95% CI=32–64)% (n=40), 37–48 weeks 75 (95% CI=51–91)% (n=20), >48 weeks 63.8 (95% CI=54.78–72.12)% (n=127). Neither housing type nor geographical location had a significant effect on the likelihood of samples being positive for antibodies to E. rhusiopathiae. CONCLUSIONS: The results of this study suggest the prevalence of erysipelas under New Zealand field conditions may be higher than expected and that the disease is significantly associated with increasing age. Housing type and geographical location appear to be unrelated to seroprevalence. CLINICAL RELEVANCE: Further study of the epidemiology of E. rhusiopathiae in chickens in New Zealand should be considered in order to minimise the extent of birds' exposure to the organism. These findings will assist in the design of further studies. 相似文献
983.
AIM: To determine the prevalence of Mycobacterium bovis infection in brushtail possums (Trichosurus vulpecula) that did not have macroscopic lesions of bovine tuberculosis, and to evaluate culture of pooled tissues from multiple possums as a method for determining the M. bovis-infection status of wildlife populations in New Zealand. METHODS: Pools of selected tissues were collected from possums from four different populations known to be infected with M. bovis. Tissue pools from individual animals, and combined pools from multiple animals, were cultured for M. bovis. RESULTS: In the four populations investigated, the prevalence of possums with macroscopic lesions confirmed by culture to be infected with M. bovis ranged from 1 to 19 (mean 31/283; 10.9)%. The prevalence of possums with non-visible lesions that were culture positive for M. bovis in the same populations ranged from 4 to 10 (mean 24/283; 8.5)%. The mean of the log10 cfu of M. bovis of the macroscopic lesions and of the culture-positive samples that did not have visible lesions was 3.85 (SE 0.26) and 1.46 (SE 0.26) log10 cfu, respectively (p<0.01). Mycobacterium bovis was cultured from pools of 30–50 animals in the four populations studied. CONCLUSIONS: The finding of M. bovis infection in possums with non-visible lesions identified a potential deficiency of declaring possum populations free of M. bovis on the basis of absence of macroscopic lesions. The culturing of pools of selected tissues from multiple animals without visible lesions can be used to reduce laboratory costs of possum surveys without a major reduction in the ability to detect M. bovis infection. 相似文献
984.
985.
[目的]探索湖北十堰麦区小麦条锈病冬繁区发展流行规律,使国家小麦主产区免受条锈病危害,保障全国夏粮增产丰收。[方法]采用高感小麦品种铭贤169结合人工接种的方法,在湖北十堰选取4个县市开展小麦条锈病定点接种和自然诱发监测试验。[结果]4个监测点接种后均发病扩展,未接种的自然诱发圃也显症发病;条锈病病菌冬季在十堰市辖区范围可以缓慢冬繁,潜育期随着海拔的升高潜伏期相应延长;早春阶段条锈病病菌在十堰市各县市可进行再侵染,但因气温低扩展缓慢,显症不明显。[结论]十堰市小麦条锈病春季菌源大部分为甘、陕及西南地区和本地冬繁的菌源。 相似文献
986.
为了解猪流感病毒(SIV)在我国的流行情况,本研究对2011年~2012年从不同省市重点地区屠宰场、规模化猪场和个体养殖户采集猪血清样品13044份,以经典H1N1 (CS)、类禽H1N1 (EA)、甲型H1N1(pdm/09)、H3N2、H5N1、H9N2亚型SIV抗原并采用血凝抑制(HI)方法检测.结果显示,2011年~2012年我国猪血清中类禽H1N1亚型SIV抗体平均阳性率比其他亚型高,分别为42.24%和45.89%,2011年甲型H1N1亚型SIV血清抗体平均滴度(GMT)比其他亚型高,GMT为53.32,2012年经典H1N1 GMT比其他亚型高,GMT为73.2.结果表明2011年~2012年我国猪群中H1N1亚型SIV感染较为普遍. 相似文献
987.
H. C. So D. L. Pearl T. von Königslöw M. Louie L. Chui L. W. Svenson 《Zoonoses and public health》2013,60(5):341-348
Molecular typing methods have become a common part of the surveillance of foodborne pathogens. In particular, pulsed‐field gel electrophoresis (PFGE) has been used successfully to identify outbreaks of Escherichia coli O157:H7 in humans from a variety of food and environmental sources. However, some PFGE patterns appear commonly in surveillance systems, making it more difficult to distinguish between outbreak and sporadic cases based on molecular data alone. In addition, it is unknown whether these common patterns might have unique epidemiological characteristics reflected in their spatial and temporal distributions. Using E. coli O157:H7 surveillance data from Alberta, collected from 2000 to 2002, we investigated whether E. coli O157:H7 with provincial PFGE pattern 8 (national designation ECXAI.0001) clustered in space, time and space–time relative to other PFGE patterns using the spatial scan statistic. Based on our purely spatial and temporal scans using a Bernoulli model, there did not appear to be strong evidence that isolates of E. coli O157:H7 with provincial PFGE pattern 8 are distributed differently from other PFGE patterns. However, we did identify space–time clusters of isolates with PFGE pattern 8, using a Bernoulli model and a space–time permutation model, which included known outbreaks and potentially unrecognized outbreaks or additional outbreak cases. There were differences between the two models in the space–time clusters identified, which suggests that the use of both models could increase the sensitivity of a quantitative surveillance system for identifying outbreaks involving isolates sharing a common PFGE pattern. 相似文献
988.
The objective was to determine factors influencing social organization in postpartum Angus cows and its effect on variation of cow–calf weight after 90 d. Postpartum Angus cows (N=90) with no history of puerperal problems, a calf suckling, and body condition score (BCS) no less than 3 were allocated into 3 groups of 30 (A, B, and C) according to parity (P) and cow body frame (CF). Using the agonistic interactions recorded during the study, a raw dominance value with subsequent Arc-sin conversion (Arc-sin DV) was calculated to generate a linear social dominance order (DO) with 3 social categories (dominant—D-, intermediate—I-, and subordinate—S-cows). The effects of postpartum body weight (PPBW), BCS, CF, and P on Arc-sin DV were statistically analyzed using ANOVA. Similar procedure was applied to analyze the effects of group, P, CF, and DO on cow body weight at 90 d (CW90), and the effects of DO of dams, P, group, calf gender (CG), and sire within breed (SWB) on calf body weight at 90 d (CFW90). Cows with larger CF and heavier PPBW obtained greatest (P<0.0005) Arc-sin DV. The youngest and the oldest cows tended (P<0.10) to obtain the lowest Arc-sin DV. After 90 d of trial, D cows were heavier than I (P<0.002) and S cows (P<0.0001). Calf birth weight was influenced by SWB (P<0.03) and P (P<0.05), and D cows had heavier (P<0.05) calf at birth than S cows. At 90 d, calf body weight was influenced by P (P<0.03) and DO of dam (P<0.008). Thus, calf weight at 90 d increased with DO of dams. In conclusion, CF, PPBW, and to a less extent P, influenced DO. Parity and CF influenced PPBW. Variation of CW90 was influenced by DO. Calf birth weight was influenced by SWB, P, DO of cow, and CG. Meanwhile, CFW90 was influenced by P and DO of cow. Interactions among social organization, management strategies, calf growth, and reproductive aspects need more attention. 相似文献
989.
990.
在我国,司法与媒体往往是亦友亦敌的关系,司法公正需要媒体监督,而媒体滥用监督权又会损害司法公正的实现.通过媒体监督与司法公正的内涵,媒体监督对司法公正的影响,以及正确处理两者的关系等问题的探讨,探求媒体与司法良性互动的途径,以达保障司法公正的目的. 相似文献