Quick detection of sex determining region protein gene in mouse fetuses

AIM: To detect quickly the Y-chromosome specific sex determining region protein (Sry) gene in mouse fetuses on embryonic day 14.5 with a PCR method. METHODS: We designed specific primers with the OLIGO 5. 0 software. Templates were prepared in 30 minutes by the following way. About 1 mg embryonic tissue but not fetal liver was suspended, and treated with 200μL of lysis buffer, consisting of PCR buffer containing 20 mg/L proteinase K, 0. 5% NP-40, and 0.05% Tween 40, at 60°C for 15 minutes, heated for 5 minutes at 100 °C, 10μL was used as template. The PCR react ion was performed in 50μL, using two sets of primers specific for Sry gene (chromosome Y) and IL-3 gene (chromosome 11) . PCR conditions and cycle numbers were optimized. The assessment of the results was done by electrophoresis in 3% agarose run at high voltage. The specificity of the method was conf irmed by fluorescent in situ hybridization (FISH) using a specific male probe on embryonic tissue cells. RESULTS: Electrophoresis showed that PCR product of male control DNA consisted of a 649 bp product representing the IL-3 gene and a 444 bp product representing the Y-specific Sry gene, female control DNA only one 649 bp product. Fetuses with two bands matching those as seen inmale control DNA are the presumpt ive male fetuses. Fetuses, only the IL-3-associated 649 bp band, are the presumptive female fetuses. These were confirmed by FISH. The ent ire procedure took <3. 5 h. CONCLUSION: The established PCR assay offers a quick, simple, accurate, and sensitive detection of sex determining region protein gene in mouse fetuses. This method allowed the preparation and culture of pure male and female hematopoietic stem cells from fetal tissue.     

云南桔小寡鬃实蝇种群动态研究

经过水平和垂直地带的性诱观测与室内外调查试验，发现云南桔小寡鬃实蝇年发生４－５代。第二代为主害代，主要为害芒果、桃、番石榴等。成虫诱集高蜂期除柑桔点（高峰期为９－１０月）、甜橙点（高峰期为１０－１２月）外，蓁各点均在６－７月间。各年高峰期早晚和诱集量不同。同年各点成虫高峰期出现的时间也有差异，主要表现为从南到北高峰期推迟。冬季除昆明外，其它各点均可诱到成虫。以高温高湿的西双版纳数量最多。同一地区随     

松褐天牛引诱剂林间对比试验

在重庆地区进行的不同引诱剂对松褐天牛的诱捕试验结果表明,3种引诱剂对松褐天牛都有一定的引诱效果。其中,引诱剂M-99的效果最好,引诱活性平均为46.60头/诱捕器;引诱剂A-3引诱活性平均为37.00头/诱捕器;FJ-Ma-05最弱,平均为16.20头/诱捕器;且引诱剂M-99在6—7月的引诱活性均高于其他药剂。根据试验中松褐天牛的种群变化规律可知,6—7月是防治松褐天牛的最佳时期。     

4种不同性诱剂对玉米草地贪夜蛾诱集作用

为准确掌握草地贪夜蛾成虫发生动态,筛选对当地种群专一性强、灵敏度高的性诱剂,对深圳百乐宝生物农业科技有限公司(BLB)、宁波纽康生物技术有限公司(NK)、江苏宁录科技股份有限公司(NL)、南京新安中绿生物科技有限公司(XAZL)4个厂家生产的草地贪夜蛾性诱剂在江苏盐城市大丰区进行田间诱捕效果试验,探讨草地贪夜蛾性诱剂的使用技术。结果表明:4种不同性诱剂诱芯对草地贪夜蛾的诱集效果存在明显差异。BLB诱芯和NL诱芯可以有效监测草地贪夜蛾成虫田间发生动态,羽化高峰期明显,且峰值日期一致,NK诱芯诱集的成虫虫峰不明显,XAZL诱芯零星诱到成虫,无明显成虫高峰;BLB诱芯监测期内日均诱蛾量、最高单次诱蛾量、总诱蛾量均显著高于其他3种诱芯,其总诱蛾量达136.75头,分别是NL诱芯、NK诱芯、XAZL诱芯的1.47倍、4.76倍、10.52倍;通过对BLB诱芯进行持续30 d和持续60 d的诱捕对比试验,发现BLB诱芯1~30 d内诱捕性能稳定,诱蛾量大,31~60 d内日均诱蛾量、最高单次诱蛾量、总诱蛾量均有所下降且达显著水平,尤其是50 d后诱捕量下降明显。结果表明:BLB诱芯的诱蛾量大,能够明显反映出草地贪夜蛾的羽化高峰期,符合测报要求,更适合本地区草地贪夜蛾的监测,使用该诱芯时建议30 d更换1次。     

桑毛虫性信息素研究 Ⅲ.性诱测报试验

采用我们自行研究合成的桑毛虫性信息素(顺—7—十八碳烯醇异戊酸酯)聚乙烯塑料诱芯进行桑毛虫发生期的测报,巳获得成功。试验结果表明,性诱发蛾进度与田间产卵进度具有极显著的相关关系,性诱蛾量能比较真实地反映田间成虫的实际发生情况.用性诱蛾量高峰日预测成虫的产卵高峰和次代幼虫的孵化高峰期,解决了性诱测报中预先无法估计50％发蛾高峰期的难题,这对指导测报和防治具有十分重要的现实意义。与传统的黑光灯诱蛾测报法比较,性诱测报具有准确性高,方法简便,成本低廉等优点,是一项值得推广的新的测报方法。     

应用松褐天牛引诱剂进行林间监测技术的研究

本文就应用松褐天牛ＭｏｎｏｃｈａｍｕｓａｌｔｅｒｎａｔｕｓＨｏｐｅ引诱剂监测松褐天牛在林间的种群密度、成虫携带松材线虫Ｂｕｒｓａｐｈｅｌｅｎｃｈｕｓｘｙｌｏｐｈｉｌｕｓ（ＳｔｅｉｎｅｒｅｔＢｕｈｒｅｒ）Ｎｉｃｋｌｅ病原状况进行了探讨。结果表明：在松材线虫病病区外围设置引诱剂能及时发现松褐天牛携带松材线虫病原及扩散的区域。按１０００ｍ２设置１个诱捕器的网格状布局，该引诱剂的诱捕率为１８．９３％     

诱捕器引诱松墨天牛试验

2002~2004年在厦门市的同安区西山、郭山马尾松纯林设置试验地,采用FJ-MA-02松墨天牛引诱剂对松墨天牛进行引诱。结果表明:4~6月诱虫量占整个诱虫量的83.3%,诱虫高峰期在5月。西山每个诱捕器2002~2004年每年平均分别引诱到60.6头、29.8头和12.2头,平均每667 m2林地引诱到1.9头、0.9头和0.5头。诱虫量分别比上一年下降了50.8%、59.1%。郭山每个诱捕器分别引诱到47.8头、20.6头和11.5头,平均每667 m2引诱到1.3头、0.5头和0.3头。诱虫量分别比上一年下降了57.0%、44.2%。说明引诱剂对降低松墨天牛种群密度,控制松墨天牛危害能发挥重要作用。     

白蜡虫优良种虫及种群性比组成的研究

白蜡虫(Ericerus pela Chavannes)是我国特产资源昆虫,国内产区很多。实验表明各产区繁育白蜡虫的产量与质量差异显著,其中以金沙江下游云、贵、川三省相接壤的白蜡虫产区,繁育的白蜡虫产卵数量多、雄卵比率高、产蜡时间长、产蜡量多,同时蜡虫的产量高,是我国白蜡虫的优良种虫。     

Effects of photoperiod on sex steroids and gonad maturation in Arctic charr

Four groups of Arctic charr (age 3+), previously exposed to natural photoperiod, were subjected to different photoperiod regimes from February 20: Simulated natural photoperiod (SNP; Tromsø 70°N), continuous light (LD 24:0) followed by short day (LD 6:18) from either May 1 (L_Feb–May) or June 25 (L_Feb–June), or continuous light (LD 24:0) throughout the experiment (L_Continuous). In females, peak levels of estradiol-17β and testosterone were 6 and 10, 6 and 7, and 3 and 5 weeks advanced, in L_Feb–May, L_Feb–June and L_Continuous, respectively, whereas in males plasma levels of 11-ketotestosterone peaked 8 weeks earlier in L_Feb–May and L_Feb–June than in SNP and L_Continuous. Median ovulation time in L_Feb–May and L_Feb–June was advanced by 10 weeks compared to SNP and L_Continuous, and median spermiation time (first observation of running milt in each individual) in L_Feb–May, L_Feb–June and L_Continuous was advanced by 10, 7 and 5 weeks, compared to SNP. A switch from long to short day early in the reproductive cycle (L_Feb–May and L_Feb–June) apparently resulted in stronger synchronization of both ovulation and spermiation. Ovulation in L_Feb–May and L_Feb–June occurred over 3 weeks (L_Feb–May) and 8 weeks (L_Feb–June), as compared to 10 weeks in the SNP group, whereas all males (except one individual) began spermiating over a period of 3 weeks (L_Feb–June), or less (L_Feb–May), as compared to 7 weeks in the SNP group. Lack of a short day stimulus (L_Continuous), on the other hand, resulted in larger variation in the timing of final maturation, with ovulation and spermiation being spread over 15 weeks. Low egg survival in L_Feb–May (17% versus 60% and 97% for L_Continuous and SNP) indicate that a long day signal of 10 weeks early in the reproductive cycle may be too short to ensure good egg and sperm quality. Photoperiod manipulation did not affect the proportion of maturing fish.     

Effects of sex ratio, stocking density and age of hybrid tilapia on seed production in concrete tanks in Saudi Arabia

The influence of sex ratio, stocking density and age of hybrid tilapia (Oreochromis niloticus X O. aureus) brood fish on seed production in concrete tanks were evaluated in 1992 and 1993. A sex ratio of 1:2 and 1:3 (male: female) and a density of 2 fish m-2 gave the best result with respect to number of seed female-1 day-1 and number of seed kg-1-1 female day-1. Maximum seed production female-1 day-1 was recorded for 3-year-old females and the minimum for 1-year-olds, but in terms of seed kg-1 female day-1 and seed m-2 day-1, 1-year-olds produced the maximum number of seed followed by 2-, 3- and 4-year-old females. Seasonal pattern in seed production was apparently influenced by season and temperature. From the first harvest on 21 April 1993 the production increased gradually, reaching a peak during the third harvest and a second peak during the sixth harvest. Thereafter seed production gradually decreased, reaching a minimum during the tenth harvest on 27 October 1993. No seed were found during the last harvest of 18 November 1993. Maximum production coincided with the highest water temperature of around 28°C.