Bioinformatics Analysis of Bone Morphogenetic Protein 4 (BMP4) of Sheep

The assay was aimed to explore the biological characteristics of bone morphogenetic protein 4 (BMP4) of sheep,NCBI,DNAMAN DNAStar,TMHMM Server v.2.0,PsortⅡ,SignalP various bioinformatical softwares were used to speculate the physical and chemical properties,hydrophobic property,phosphorylation site,conservative structure domain,protein secondary structure of BMP4 protein.Also,the three-dimensional structure was forecasted with the SWISS-MODEL Workspace software.The results indicated that the BMP4 of sheep had high homologies with the BMP4 of various species.The encoded protein was a hydrophilic protein which was unstable.There was no transmembrane regions and it was likely to be located in the nucleus.What was more,there was signal peptide and eighteen phosphorylation sites.Through the forecast of functional domains,the protein had two functional domains,including the transforming growth factor-beta (TGF-beta) superfamily and TGF-beta propeptide superfamily.The result was consistent with the function of BMP4 gene family,it also demonstrated that BMP4 was a growth factor and it had the function of signal transduction.The amino acid homology between the predicted 3D structure of protein and template 3bmp.1.A was 88.29%.The bioinformatics analysis of BMP4 gene could provide reference for the further study in practice.     

基于Lasso和BP神经网络的组合预测及其应用——以居民消费支出预测为例

在变量选择的基础上，构建基于Lasso方法和BP神经网络的预测模型，并对我国城乡居民的消费支出进行预测，结果显示：基于Lasso方法和BP神经网络的组合预测精度要明显高于BP神经网络、Lasso方法的预测精度；在2014~2020年，我国农村居民消费增长率有所提升，城镇居民消费增长率减缓，城乡居民消费增长率之间的差距呈下降趋势，但短期内城乡居民消费差距依然难以缓和。     

河南卫河流域脆弱性评价

结合卫河自身特点和地理环境建立13项指标体系,运用基于熵权的模糊数学模型对其脆弱性进行评价。结果表明,鹤壁的脆弱性为0.69,焦作为0.55,新乡为0.39,安阳为0.36,濮阳为0.23。最后针对评价结果分析了原因,提出了合理性建议和措施。     

浅析科学施肥

肥料对农作物增长、稳产、高产非常重要,就科学施肥的意义以及施肥中常见的问题做以分析,提出科学施用化肥方法,提高肥料利用率。     

Effect of Psammaplin A on in vitro Development of Bovine Aging Oocytes

To investigate the effect of histone deacetylation inhibitor Psammaplin A (PsA) on the development of bovine aging oocytes in vitro,oocytes were randomly divided into control group,aging group and 50 mmol/L PsA treated aging group (PsA group).Immunofluorescence staining and JC-1 were used to detect the blastocyst rate of bovine oocytes after parthenogenetic activation,the number of cells in blastocysts,apoptosis,reactive oxygen species (ROS),glutathione (GSH) and mitochondrial membrane potential intensity of embryos.The results showed that the blastocyst rate of the aging group was significantly lower than that of PsA and control groups (P<0.05).The blastocyst rate of PsA group was not significantly different from that of control group (P>0.05).The number of cells in the blastocysts of control group and PsA group were significantly higher than that of aging group (P<0.05).The number of cells in the blastocysts of PsA group was not significantly different from that of control group (P>0.05).The apoptosis rate in aging group was significantly higher than that of control and PsA groups (P<0.05),the apoptosis rate of PsA group was significantly higher than that of control group (P<0.05).The GSH level of MⅡ oocytes in aging group was significantly lower than that of control and PsA groups (P<0.05).There was no significant difference in GSH level between control and PsA groups (P>0.05).The ROS level of the embryos in aging group was significantly higher than that of control and PsA groups (P<0.05).The ROS level in PsA group was significantly higher than that of control group (P<0.05).The mitochondrial membrane potential of early embryos of aging group 4-8 cells was significantly lower than that of control and PsA groups (P<0.05).The mitochondrial membrane potential intensity of control group was significantly higher than that of PsA group (P<0.05).In summary,PsA could effectively delay the aging of bovine oocytes and improve the quality of oocytes.     

房地产开发企业动态能力的维度结构与实证研究

羊群行为属于行为经济学的一部分，认为市场主体在信息环境不确定的情况下，其行为易受到其他参与主体的影响，模仿他人决策。基于Hwang和Samlton羊群行为测度方法（HS方法）及EGARCH模型，运用2003年1季度至2014年3季度我国30个省市房地产市场销售价格数据，验证我国房地产市场羊群行为的存在性，并分析购房者羊群行为对中国房地产价格波动的影响。结果表明：我国房地产市场羊群行为显著存在，且购房者羊群行为与房地产价格波动呈正相关关系。     

自动折叠喷洒臂的设计及运动学特性

为了解决当前卷盘式喷灌机所配套的喷灌车自动化水平较低的问题,设计了一种基于电动缸驱动的自动折叠喷洒臂,并在喷洒臂之间连接处设计了一种当喷洒臂完全展开时能够实现自密封的端面密封铰链.基于喷洒臂完全折叠时收拢于车架两侧,完全伸展时铰链左右铰合密封等特征建立各杆件参数约束方程,确定了喷洒臂的各杆件尺寸.通过矢量方程法对喷洒臂进行运动学分析,以电动杆推杆的转角、位移、速度、加速度为输入量,各杆件的角位移、角速度、角加速度为输出量,通过Simulink搭建喷洒臂的运动仿真框图并进行分析.结果表明:完全折叠时,喷管1与水平方向呈90°夹角;伸展过程中,各喷管运动平稳,且始终保持在水平直线方向;完全展开时,3个喷管转动90°,且各喷管铰接处完全闭合不发生水泄漏现象,说明设计具有一定的合理性.     

高职院校《国际市场营销》课程案例教学法的运用

在高职院校《国际市场营销》课程教学中采用案例教学法是高职教育培养高素质、国际化人才的必然要求。文章首先阐述了《国际市场营销》课程中运用案例教学法的意义，并分析了当前该课程运用案例教学法普遍存在的问题，最后有针对性地提出了运用与实施该教学方法的条件和要求。     

两个新的黄瓜SnRK2基因的鉴定及特征分析

植物SnRK2基因在非生物逆境应答中具有重要功能,但有关黄瓜SnRK2基因的研究尚缺乏报道。笔者通过对黄瓜基因组的重新挖掘,发现了2个新的SnRK2基因,分别与拟南芥的SnRK2.4和SnRK2.10同源。本试验对2个新的黄瓜SnRK2基因的结构特征及顺式调控元件进行了全面分析,为进一步研究其在黄瓜逆境应答中的功能奠定了基础。