鱼肠道弧菌免疫检测方法的研究

采用灭活的鱼肠道弧菌作为抗原,制备兔抗血清,建立了鱼肠道弧菌的间接荧光抗体检测技术(indirect im-munofluorescence assay test,IFAT)、Western-blotting免疫印迹检测技术。IFAT结果显示阳性信号覆盖整个菌体,明亮,清晰,荧光信号呈长弧形且两端钝圆,与鱼肠道弧菌形状一致。Western-blotting结果显示共10条蛋白带发生免疫反应,其中6条蛋白带结果较清晰,显示出较强的特异性免疫反应,阴性对照组无条带。IFAT和Western-blotting结果说明所建立检测方法能够准确、快速的检测出鱼肠道弧菌。     

机械化直播油菜的优点及高产栽培技术

针对近年来油菜生产机械化程度低、用工多、成本高、效益低、面积逐年萎缩的情况,通过多年试验与总结,探索与完善了机械化直播油菜的高产栽培技术,实现了省工节本、提高机械化水平的目的,也为大面积油菜生产提供技术参考。     

杂交稻三香优410的特征特性及栽培技术

三香优410在建瓯种植,表现出丰产性好、抗性较强、米质优良等特性,栽培上要注意选好秧田、培育壮秧、合理密植、科学施肥、做好水管、病虫防治。     

农机与农艺结合中存在的问题及对策

农机与农艺紧密结合,对于农业经济发展具有重要意义,是未来农业发展的必然趋势。阐述农机与农艺结合的重要性,分析农机与农艺结合过程中存在的问题,提出加快农机与农艺结合的有效措施,以期为现代农业的发展提供参考。     

封闭型植物工厂发展现状与展望

经过多年探索和发展,封闭型植物工厂无论在材料、装置和技术都取得了快速进步,生产产量成数倍增长。在介绍发展封闭型植物工厂必要性的基础上,着重分析了封闭型植物工厂的发展现状、设施分类、栽培形式选择、栽培技术选择,最后指出了存在的问题,并对未来的封闭型植物工厂外部设施及其栽培技术进行了展望。     

深松整地作业技术要点

根据康平县在实施深松整地作业中遇到的实际问题,介绍深松机具的选择原则、作业质量标准、作业时间确定方法,以及作业技术要点和注意事项,为提高全县深松整地作业质量提供参考。     

Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects

The aim of this study was to develop a novel oil-in-water (O/W) emulsion as adjuvant diluents (AD) for live vaccine against classical swine fever (CSF) that could effectively enhance the immune effect of vaccine.The AD was prepared by high-pressure homogenization technique.Formulations and preparation parameters were optimized with response surface design.Its stability, particle size, polydispersity (PDI) and Zeta potential were characterized.The humoral immune response and cellular immune response of the AD were evaluated with BALB/c mice by intramuscular injection.The particle size of the AD prepared by optimized formulation and parameters was 100.4 nm, PDI was 0.147, and Zeta potential was —28.7 mV.The experiment results showed that the AD had good stability.The AD was inoculated combined with live vaccine against CSF into BALB/c mice by intramuscular injection.The results showed that the live vaccine against CSF specific immune responses could be evoked in mice by co-inoculation with AD and vaccine.The cellular immune response levels in co-inoculated groups were significantly higher than control group (P<0.05), with obvious phenomena of higher levels of IFN-γ, IL-6 and IL-4 in serum.The result revealed that cellular immune capability significantly improved with the AD.The results strongly revealed that cellular immune capability significantly improved by introducing AD for effective immune-adjuvant for live vaccine against CSF.     

中浙优8号在沙县作烟后稻种植表现及高产栽培技术

中浙优8号是中国水稻研究所和浙江勿忘农种业股份有限公司采用优质不育系中浙A与恢复系T-8配组而成的优质高产迟熟三系杂交稻新品种,经过3 a在沙县作烟后稻示范种植,表现群体整齐、分蘖力强、结实率高、米质较优、丰产稳产性好等特点。介绍其在沙县示范表现及高产栽培技术。     

水稻两段栽培技术在浉河区生产上的应用成效

介绍了水稻两段栽培技术的原理和方法,以及信阳市浉河区推广水稻两段栽培技术在减肥控氮、节本增效等方面取得的主要成效和主要做法。     

番茄树在百色的引种表现及栽培技术

从育苗、定植前准备、定植、田间管理、病虫害防治、采收等方面对农业观光园番茄树京丹六号栽培技术进行总结。