微生态复合菌制剂在对虾养殖中的应用研究

以芽孢杆菌和乳酸菌为主体菌,并辅以放线菌组成的微生态复合菌制剂用于对虾养殖中,探讨了它们对养殖水体理化因子、生物因子和对虾生长的影响。模拟试验结果表明,投加微生态复合菌制剂后,试验池6dCODCr去除率高达84.7%;迅速矿化有机氮,有效加强硝化-反硝化作用,控制NH4-N在一定水平,NO2-N去除率高达81.5%;pH稳定在8.0～8.3之间,DO保持在5mg·L-1的较高水平。虾池长期监测结果表明,试验池水质各项指标均优于对照池;无任何大规模虾病发生;未出现“耍食”现象。试验池比对照池增产近1倍,成本降低近80%。     

五叶地锦离体培养及植株再生

以五叶地锦(Parthenocissus quinquefolia)子叶柄、下胚轴、叶片为外植体进行离体培养,以1/2 MS、B5和Heller为基本培养基,附加不同浓度的细胞分裂素(6-BA,KT, TDZ)及生长素(NAA)诱导下胚轴直接再生不定芽.实验结果表明,暗处理30 d,靠近下胚轴的子叶柄接种在1/2 MS 0.3 mg·L-16-BA培养基上,其不定芽分化率最高达64.67%;下胚轴接种在1/2 MS 2.0 mg·L-1KT 0.05 mg·L-1NAA和Heller 0.5 mg·L-1BA 0.1 mg·L-1NAA培养基上,其不定芽分化率接近,达到24%左右;无菌苗的叶片接种在B5 0.5 mg·L-1TDZ 0.05 mg·L-1NAA培养基上,其不定芽分化率达17.7%.芽增殖培养基为1/2 MS 0.5 mg·L-16-BA 0.1 mg·L-1NAA增殖系数为3～5, 1/2 MS 0.5 mg·L-1IBA 500 mg·L-1活性炭适于再生幼苗的生根,生根苗经移栽全部成活.     

Hydrogen sulfide ameliorates high glucose-induced endothelial cell senescence by suppressing oxidative stress

AIM：To explore the effect of hydrogen sulfide on the senescence of human umbilical vein endothelial cells (HUVECs) induced by high glucose. METHODS：Senescence model was established by treating HUVECs with 33 mmol/L glucose for 48 h. The parameters were detected to demonstrate the effect of hydrogen sulfide on senescence and the mechanism involved was also investigated. RESULTS：In the cells treated with high glucose, the proliferation was attenuated with a higher number of senescence-associated β-galactosidase (SA-β-Gal) positive cells, and plasminogen activator inhibitor 1 (PAI-1) protein expression, malondialdehyde (MDA) production and NF-κB p65 activity were increased significantly, but the expression of superoxide dismutase 1 (SOD1) was decreased. However, the cell number and SOD1 expression were increased, and the number of SA-β-Gal positive cells, PAI-1 protein expression, MDA production and the activity of NF-κB p65 were decreased after sodium hydrosulfide (100 and 200 μmol/L) treatment.CONCLUSION：Exo-genous hydrogen sulfide prevents HUVECs against high glucose-induced senescence by suppressing oxidative stress and NF-κB p65 activity.     

miR-140-3p inhibits viability,invasion and migration of non-small-cell lung cancer A549 cells by targeting PD-L1

AIM: To explore the target relationship between microRNA-140-3p (miR-140-3p) and programmed cell death ligand 1 (PD-L1) and their effect on the viability, migration and invasion of non-small-cell lung cancer A549 cells.METHODS: RT-qPCR was used to detect the miR-140-3p expression in HLF-1, A549 and H1299 cells, and then the A549 cells with the most significant difference were selected as the subsequent research object. TargetScan software and dual-luciferase reporter assay were performed to predict and confirm the target relationship between miR-140-3p and PD-L1. RT-qPCR and Western blot were used to determine the effects of miR-140-3p mimic and inhibitor on PD-L1 expression level. MTT assay was used to detect the viability of A549 cells. Transwell assay was performed to detect the migration and invasion abilities of the A549 cells.RESULTS: miR-140-3p was significantly down-regulated in the A549 cells and H1299 cells (P<0.05). Transfection with miR-140-3p mimic decreased the expression of PD-L1 and inhibited the viability, migration and invasion of the A549 cells. Transfection with pcDNA3.0-PD-L1 reversed the inhibitory effect of miR-140-3p on the viability, migration and invasion of the A549 cells.CONCLUSION: miR-140-3p inhibits the viability, migration and invasion of A549 cells by targeting PD-L1.     

热研4号王草干燥特性的试验研究

在将热研4号王草调制成青贮饲料或干草的加工中,干燥是一个关键性环节,也是发展热研4号王草产业的瓶颈问题。为此,采用快速薄层干燥的试验方法,研究热研4号王草的含水量与干燥时间、干燥介质温度两者之间的关系,并分析影响其营养成分的因素。结果表明:热研4号王草的干燥最佳介质温度为150°～180℃,鲜草刈割后必须在24h内进行快速干燥。     

低氧胁迫下鲢miR-17a-5p及其靶基因分析

为了探究miR-17a-5p在鲢低氧胁迫下的功能,在前期鲢small RNA测序的基础上对鲢miR-17a-5p进行靶基因预测及功能富集分析,通过双荧光素酶活性验证其与HIF-1α的靶向关系,并检测低氧胁迫下miR-17a-5p和其靶基因在鲢肝脏、脑、心脏和鳃四个组织中的动态表达特征。结果显示,鲢miR-17a-5p在不同物种间高度保守,预测出381个miR-17a-5p的潜在靶基因显著富集在硫代谢、mTOR信号通路以及萜类骨架的生物合成3个KEGG信号通路上。miR-17a-5p可与HIF-1α mRNA的3′UTR结合,并降低HIF-1α mRNA水平,低氧胁迫下miR-17a-5p的表达呈下降趋势,而HIF-1α表达则呈上升趋势;3个显著富集KEGG通路中的11个miR-17a-5p潜在靶基因在低氧胁迫过程中的不同组织中的表达,尤其是肝脏中的表达,除SGK1外,均呈显著上升趋势。研究表明,低氧胁迫下鲢各组织中miR-17a-5p的表达下调减弱了其对靶基因的抑制作用,进而导致HIF-1α、ddit4和Lrp5等响应低氧胁迫的基因表达上调。本研究为低氧胁迫下鲢miRNA的表达与调控机...     

半B-（p，r）-预不变凸函数与非线性规划问题

定义了一类重要的非凸函数——半B-（p,r）-预不变凸函数,它是半预不变凸函数的真推广．首先用例子说明了此类函数的存在性,并说明它是B一不变凸函数、半预不变凸函数和B-（p,r）-预不变凸函数的推广;然后,讨论了半B-（p,r）-预不变凸函数的基本性质与集合刻画,并给出了半B-（p,r）-预不变凸规划问题的非可微最优性条件,其结论具有一般性,推广了涉及不变凸函数、半预不变凸函数和B-（p,r）-预不变凸函数的一些结论．     

MiR‐330‐5p negatively regulates ovine preadipocyte differentiation by targeting branched‐chain aminotransferase 2

The differentiation of preadipocytes into adipose tissues is tightly regulated by various factors including microRNAs and cytokines. This article aims to study the effect of miR‐330‐5p on expression of BCAT2 in ovine preadipocytes. Ovine preadipocytes were isolated, and we found that the miR‐330‐5p expression decreased gradually during the early differentiation of ovine preadipocytes, while BCAT2 expression increased. BCAT2 was identified as a direct target of miR‐330‐5p, ectopic expression of miR‐330‐5p could change the expression of both BCAT2 mRNA and protein. Silencing BCAT2 had the same inhibition effects as overexpressing miR‐330‐5p on the preadipocyte differentiation, but overexpressing BCAT2 had the converse effects. Taken together, we demonstrated that miR‐330‐5p is a negative regulator of differentiation by targeting BCAT2, and clarified the role of BCAT2 and miR‐330‐5p during preadipocyte differentiation.     

绵羊肺炎支原体 p113 基因的序列分析及功能预测

采用多种软件对p113基因的相似性、跨膜结构、重复序列、信号肽、抗原表位等进行预测,并与同源序列进行比较分析.结果表明,p113基因是猪肺炎支原体黏附素基因p97的直系同源基因,并具有与P97蛋白R2区类似的特征性重复序列.通过综合比较分析,推测P113蛋白极可能是绵羊肺炎支原体的黏附素和膜表面免疫原.     

Ethanol extract from Cortex Albiziae attenuates mouse acute liver injury induced by carbon tetrachloride via modulation of NF-κB pathway

AIM:To investigate the protective effect of ethanol extract from Cortex Albiziae on acute liver injury, and to explore its possible mechanism. METHODS:Acute liver injury in mice was induced by single intraperitoneal injection of 25% carbon tetrachloride (olive oil solubilization). The effective parts of ethanol extract from Cortex Albizziae against acute liver injury were screened. The pathological changes of the liver tissues were examined by pathological sections with HE staining. The activity of total superoxide dismutase (T-SOD) and the content of malondialdehyde (MDA) of the liver tissues were detected, the serum levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were mea-sured by ELISA, and the protein expression levels of NF-κB p65, Bcl-2 and Bax in the liver cells of the mice in each group were determined by Western blot. RESULTS:Compared with model group, the serum levels of AST and ALT in low-dose n-butanol phase of ethanol extract from Cortex Albiziae (AB-L, 4 mg·kg^-1·d^-1) group and high-dose n-butanol phase of ethanol extract from Cortex Albiziae (AB-H, 8 mg·kg^-1·d^-1) group were significantly decreased. The necrosis extent and degree of the hepatocytes and infiltration of inflammatory cells were significantly lower than that in model group. Compared with model group, the serum levels of TNF-α and IL-6 in AB-H group and AB-L group were significantly decreased (P<0.05). The protein level of NF-κB p65 in the nuclei of mouse liver cells in AB-H group and AB-L group were also decreased significantly (P<0.05). Compared with model group, the protein expression of Bax was decreased, the protein expression of Bcl-2 was increased, and the Bcl-2/Bax ratio was increased in AB-L group and AB-H group. CONCLUSION:The n-butanol phase of ethanol extract from Cortex Albiziae may protect the liver by reducing the activation of NF-κB p65, inhibiting the excessive release of inflammatory cytokines IL-6 and TNF-α, and decreasing hepatocyte apoptosis via regulating Bcl-2 and Bax expression.