Effects of 2 concentrations of sodium citrate on coagulation test results, von Willebrand factor concentration, and platelet function in dogs

BACKGROUND: Blood collection tubes containing 3.2% (0.109 M) sodium citrate, instead of 3.8% (0.129 M) sodium citrate, have recently become available in the United States. These tubes are visually indistinguishable from the traditional 3.8% sodium citrate tubes, except for wording on the label. Consequently, samples for hemostatic evaluation are frequently collected in tubes containing the lower concentration of sodium citrate. HYPOTHESIS: Results of hemostasis assays are different in samples collected in 3.2% versus 3.8% sodium citrate. ANIMALS: Twenty healthy dogs. METHODS: This study aimed at determining whether results of standard coagulation tests, von Willebrand factor concentration (vWF:Ag), and platelet function with the platelet function analyzer PFA-100a were affected by the different concentrations of sodium citrate. Blood samples were collected in tubes containing either 3.2% or 3.8% sodium citrate concentrations and processed routinely for coagulation assays (one-stage prothrombin time [OSPT], activated partial thromboplastin time [aPTT], fibrinogen concentration, and platelet count), vWF:Ag, and platelet function assays with a PFA-100. RESULTS: There was no significant difference between samples collected in 3.2% versus those collected in 3.8% sodium citrate for OSPT, aPTT, fibrinogen concentration, platelet count, or vWF:Ag. The closure times with collagen/adenosine diphosphate were significantly shorter (66 +/- 8.1 versus 74.8 +/- 9.7 seconds; P < .0001) with the 3.2% than with 3.8% sodium citrate concentration, and the hematocrit was significantly higher (47.9 +/- 5.6 versus 46.0 +/- 4.7 seconds; P = .03) in samples collected in 3.2% than in those collected in 3.8% sodium citrate. CONCLUSIONS AND CLINICAL IMPORTANCE: There is no clinically relevant effect of collection of blood into 3.2% or 3.8% sodium citrate.     

A Method of Adaptive Fuzzy Classfication on Rockmass Blast Ability

This paper presents a series of targets which are usefull for engineering.The paper also presents an adaptive weight function according to the fail mechanism of rockmass and a fuzzy aynthetic evaluation medel on rockmass blast ability.The model is applied to evaluate the blast ability of rockmass in an engineering and the result shows that the model is effective.     

Determination of Molecular Weight and Distribution of Hyroxyethl Starch (HES) by Means of GPC- RI- MALLS Technique

The author introduces a method and experimental procedures that determine dn/dc,molecular weight and its distribution of the hyroxyethyl starch(HES) by means of Gel Permeation Chromatography-Refractive index-Multi-angle laser light scattering detection(GPC-RI-MALLS)techniques.The key experimental technique and results are discussed.The method takes CH3COOH/CH3COONa as mobile phase and solvent.The experiment gets satisfactory results.It can be used as a routine method to determine the hyroxyethyl starch(HES) molecular weight and its distribution.     

A NEW METHOD OF RESEARCHINE THE SITE DUPLE FACE DYNAMIC BALANCE OF MACHINE RIGID ROTATOR BASE DON AMPLITUDE MEASUREMENT

In this paper, a new method of researching; the site dupleface dynamic balance of machine rigid rotator, namely duple test weightmethod is presented. Weight the new method against the existing method,namely single test weight method [1], both the efficiency increasing and theenergy-consumming dicreasing would be forty percent and over, with thebasic points of vibration theory, the formula of calculating weight andposition of the duple face dynamic unbalance of a rotator by means ofapplying the duple test weight is developed in detail. The method in handll-ing distinct bearing form is also discussed. Rightness of the new method isproved out by the living example of the simulant contrast testing carried outn the dynamic balance test machine and the site balance for a rotatorsystem of motor.     

活性多肽提取分离方法研究进展

综述了近几年来生物活性多肽物质的分离和提纯方法,主要包括色谱、电泳等方法,以及这些方法在肽类研究中的应用现状。     

电磁制动器性能评价体系的建立及指标权重的确定

首次建立电磁制动器性能的评价体系,重点介绍其中指标体系的确立方案及依据,通过利用层次分析法建立评价模型并对各指标进行权重的确定和层次的总排序,取得了比较客观合理的评价结果。     

基于灰色关联度变权法的浅层地下水环境质量综合评价

引入指数型白化权函数对灰色关联度变权法中的白化权函数进行修正,使得能够对地下水更合理、客观、准确地评价。将晋江市浅层地下水看作一个灰色系统,在灰色关联度变权法模型基础上,对各水质监测点不同类别的隶属度和灰色关联度作对比分析判断其评价等级以及质量优劣次序,说明灰色关联度变权法对地下水环境质量综合评价方法适当。研究区内绝大多数地区水质状况良好,影响区域地下水质量的指标主要为TDS、锰、硝酸盐、亚硝酸盐、氨氮、氯化物,水质较差区主要分布于沿海工业分布较密集区及引污水灌溉农田地区,说明工农业发展、人类活动加剧及海水入侵是造成本区水质变化的主要原因之一。     

底盘测功机等效汽车质量测试方法研究

提出了在用车工况法尾气排放测试系统中底盘测功机转动惯量的汽车等效质量的可溯源的测试方法。同美国BAR 97提供的测试方法进行了对比测试和分析,指出了BAR 97方法的不足,提高了底盘测功机加载滑行测试的准确度。     

叶用型沙棘育种研究--“蒙×中”沙棘F1代雄株变异特性与选优标准的研究

通过对蒙古沙棘亚种优良品种与中国沙棘亚种优良类型F1代雄株的变异特性分析,建立了选择优 良单株的标准,为叶用型沙棘选择育种提供了科学依据。     

南瓜叶防治菜粉蝶幼虫的药效评价及成本分析

通过实验室研究和大田试验对收集自我国民间的土农药——南瓜叶榨取液防治芥蓝菜粉蝶幼虫的药效进行验证和经济方面的分析评价。实验室研究结果表明：南瓜叶榨取液防治菜粉蝶主要作用机制是抑制其取食，在12h和24h的取食抑制中浓度FRC50（Feeding Restrain Content）分别为511．3214和484．3441mg·L^-1；12h和24h菜粉蝶幼虫对浸渍过榨取液芥蓝叶片的非选择性拒食率和选择性拒食率分别为66．00％、57．75％和56．04％、56．52％。大田试验结果表明：在第2、5、7d，榨取液对于芥蓝的保叶效果分别达到了48．17％、65．82％和70.46％；对虫害的防治效果分别达到了97．58％、86．98％和90．72％。经济分析结果显示：用土农药防治菜粉蝶，亩产芥蓝利润值最高，比不进行防治生产方式增加116．35元。