聚丙烯酰胺对干旱半干旱区不同作物水分利用及产值的影响

为了在半干旱区和干旱区农业生产中推广应用聚丙烯酰胺(polyacrylamide,PAM),在半干旱区选择了西瓜、马铃薯、玉米和谷子,在干旱区选择了春小麦、向日葵、玉米和番茄,以不施PAM为对照,测定PAM对不同作物产量、产值、水分利用效率和水分生产率的影响。结果表明半干旱区西瓜、马铃薯、玉米和谷子的产量分别提高了36.76%、24.83%、20.20%和13.16%,产值提高了36.32%,23.04%,9.37%,10.18%,水分利用效率提高了30.15%,18.83%,13.42%和6.24%,水分生产率提高了30.57%,22.97%,16.79%和11.66%。干旱区春小麦、向日葵、玉米、番茄的产量分别提高了18.56%,12.82%,13.65%和16.54%,产值提高了12.78%,10.70%,9.53%和14.71%,水分利用效率提高了13.49%,17.03%,12.96%和25.12%,水分生产率提高了16.14%,11.62%,11.79%和14.85%。干旱区春小麦的增产率和增值率虽然高于向日葵,但增产值却极显著低于向日葵。建议半干旱区应用PAM应优先选择西瓜和马铃薯,干旱区应优先选择番茄和向日葵。     

集中排水甘蔗地酸性硫酸盐土壤酸化的减缓

Recent research results suggest that acidification of acid sulfate soils may be inhibited in well-drained estuarine floodplains in eastern Australia by the absence of natural creek levees,The lack of natural levees has allowed the inuudation of the land by regular tidal flooding prior to the construction of flood mitigation work.Such physiographical conditions prevent the development of pre-draingae pyrite-derived soil acidifica-tion that possibly occurred at many levee-protected sites in eastern Australian estuarine floodplains during extremely dry spells.Pre-drainage acidification is considered as an important condition for accumulation of soluble Fe and consequently,the creation of favourable environments for catalysed pyrite oxidation.Under current intensively drained onditions,the acid materials produced by ongoing pyrite oxidation can be rapidly removed from soil pore water by lateral leaching and acid buffering,resulting in low concentrations of soluble Fe in the pyritic layer,which could reduce the rate of pyrite oxidation.     

Effect of low-molecular-weight organic acids on Cl- adsorption by variable charge soils

Low-molecular-weight (LMW) organic acids exist widely in soils and have been implicated in many soil processes.The objective of the present paper was to evaluate effect of two LMW organic acids, citric acid and oxalic acid, on Cl^- adsorption by three variable charge soils, a latosol, a lateritic red soil and a red soil, using a batch method. The results showed that the presence of citric acid and oxalic acid led to a decrease in Cl- adsorption with larger decreases for citric acid. Among the different soils Cl- adsorption in the lateritic red soil and the red soil was more affected by both the LMW organic acids than that in the latosol.     

皖南紫红色砂石岩上发育土壤的系统分类研究

对皖南不同时期紫红色砂石岩上发育的8个土壤剖面,按中国土壤系统分类体系,进行鉴别、检索、分类定名。阐述发生分类紫色土的2个亚类级土壤类型在中国土壤系统分类中归为3个土纲,即均腐土、雏形土和新成土;3个亚纲,即湿润均腐土、湿润雏形土和正常新成土;4个土类和6个亚类。提出按中国土壤系统分类的紫色土分类系统;并与美国土壤系统分类、国际土壤分类参比基础进行了参比。讨论了皖南紫红色砂石岩上发育为均腐土的成土环境条件,同时对发生分类紫色土在中国土壤系统分类类别检索提出修订建议。     

Belowground interactions between intercropped wheat and Brassicas in acidic and alkaline soils

Our previous studies showed that, under P-limiting conditions, growth and P uptake were lower in the wheat genotype Janz than in three Brassica genotypes when grown in monoculture. The present study was conducted to answer the question if P mobilised by the Brassicas is available to wheat; leading to improved growth of wheat when intercropped with Brassicas compared to monocropped wheat. To assess if the interactions between the crops depend on soil type, the wheat genotype Janz and three Brassica genotypes (two canolas and one mustard) were grown for 6 weeks in monoculture or wheat intercropped with each Brassica genotype in an acidic and an alkaline soil with low P availability (with two plants per pot). Wheat grew equally well in the two soils, but the Brassicas grew better in the acidic than in the alkaline soil. In the acidic soil, monocropped Brassicas had a 3 to 4 fold greater plant dry weight (dw) and P uptake than wheat; plant dw and P uptake in wheat were decreased or not affected by intercropping and increased in the Brassicas. In the alkaline soil, dw and P uptake of the Brassicas was twice as high as in wheat, with intercropping having no effect on these parameters. The contribution of wheat to the total shoot dw and P uptake per pot was 4-21% and 32-40% in acidic and alkaline soil, respectively. Mycorrhizal colonisation was low in all genotypes in the acidic soil (1-6%). In the alkaline soil, mycorrhizal colonisation of monocropped wheat was 62%, but only 43-47% in intercropped wheat. Intercropping decreased P availability in the rhizosphere of wheat in the acidic soil but had no effect on rhizosphere P availability in the alkaline soil. Intercropping had a variable effect on rhizosphere microbial community composition (assessed by fatty acid methylester analysis (FAME) and ribosomal intergenic spacer amplification (RISA)), ranging from intercropping having no effect on the rhizosphere communities to intercropping resulting in a new and similar rhizosphere community composition in both genotypes. The results of this study show that intercropping with Brassicas does not improve growth and P uptake of wheat; thus there is no indication that P mobilised by the Brassicas is available to wheat.     

Pyruvate dehydrogenase activity is important for colonization of seeds and roots by Enterobacter cloacae

Enterobacter cloacae is a plant-beneficial bacterium that shows promise for suppression of damping-off of cucumber and other crops caused by Pythium ultimum. We have been using a mutational approach to determine the E. cloacae genes important in bacterial-plant and bacterial-pathogen interactions in the spermosphere and rhizosphere. E. cloacae M43 is a transposon mutant of E. cloacae 501R3 that was significantly impaired in colonization of seeds and roots of diverse crop plants. Strain M43 did not increase in population on cucumber, sunflower, and wheat seeds and was significantly reduced in growth on pea seeds relative to strain 501R3. Populations of M43 were also dramatically lower than those of strain 501R3 in cucumber, pea, sunflower, and wheat rhizosphere in 42 d experiments. Molecular characterization of M43 demonstrated that there was a single transposon insertion in the genome of this strain and that this insertion was in a region of the E. cloacae genome with a high degree of DNA sequence identity with aceF. aceF encodes the dihydrolipoamide acetyltransferase subunit of the pyruvate dehydrogenase complex (PDHC). Cell lysates from strain 501R3 grown on minimal medium plus 50 mM glycerol and 2 mM acetate contained 0.011±0.0036 U pyruvate dehydrogenase activity while cell lysates from M43 grown under identical conditions contained no detectable pyruvate dehydrogenase activity. Additionally, the nutritional use profile of M43 under aerobic and anaerobic conditions was as expected for an ace mutant. Experiments reported here strongly suggest a role for aceF and the PDHC in colonization of seeds and roots of diverse crop plants by E. cloacae.     

用稀悬液Wien效应测量法来研究重金属离子与水稻土的相互作用

Interactions of three heavy metal ions, Cu²⁺, Cd²⁺, and Pb²⁺, and, for comparison, Na⁺ with electrodialytic clay fractions (less than 2 μm in diameter) of four paddy soils as well as a yellow-brown soil as a control soil were evaluated based on measurements of the Wien effect in dilute suspensions with a clay concentration of 10 g kg^?1 in four nitrate solutions of 2 × 10^?4/z mol L^?1, where z is the cation valence, and a nitric acid solution of 3 × 10^?5 mol L^?1. Field strengths ranging from 15 to 230 kV cm^?1 were applied for measuring the electrical conductivities (ECs) of the suspensions. The mean free binding energies between the various cations and all of the soils determined from exchange equilibrium increased in the order: Na⁺ < Cd²⁺ < Cu²⁺ < Pb²⁺. In general, the ECs of the suspensions in the sodium nitrate solution were smaller than those of the suspensions in the heavy metal solutions because of the lower electrophoretic mobility of sodium compared to the divalent cations. In terms of relative electrical conductivity-field strength relationships, relative electrical conductivity (REC) of suspensions containing various cations at field strengths larger than about 50 kV cm^?1 were in the descending order: Na⁺ > Cu²⁺ > Cd²⁺ > Pb²⁺ for all tested soils. A characteristic parameter of the REC-field strength curves, ΔREC₂₀₀, REC at a field strength of 200 kV cm^?1 minus that at the local minimum of the concave segment of the REC-field strength curves, characterized the strength of adsorption of the cations stripped off by the applied strong electrical field, and for all soils the values of ΔREC₂₀₀ were generally in the order: Na⁺ < Cu²⁺ ≤ Cd²⁺ ≤ Pb²⁺.     

Nitrogen fixation by Azospirillium brasilense in soil and the rhizosphere under controlled environmental conditions

Summary Acetylene reduction activity by Azospirillum brasilense, either free-living in soils or associated with wheat roots, was determined in a sterilised root environment at controlled levels of O₂ tension and with different concentrations of mineral N. In an unplanted, inoculated soil nitrogenase activity remained low, at approximately 40 nmol C₂H₄ h^-1 per 2kg fresh soil, increasing to 300 nmol C₂H₄ h^-1 when malic acid was added as a C source via a dialyse tubing system. The N₂ fixation by A. brasilense in the rhizosphere of an actively growing plant was much less sensitive to the repressing influence of free O₂ than the free-living bacteria were. An optimum nitrogenase activity was observed at 10 kPa O₂, with a relatively high level of activity remaining even at an O₂ concentration of 20 kPa. Both NO _inf3 ^sup- and NH _inf4 ^sup+ repressed nitrogenase activity, which was less pronounced in the presence than in the absence of plants. The highest survival rates of inoculated A. brasilense and the highest rates of acetylene reduction were found in plants treated with azospirilli immediately after seedling emergence. Plants inoculated at a later stage of growth showed a lower bacterial density in the rhizosphere and, as a consequence, a lower N₂-fixing potential. Subsequent inoculations with A. brasilense during plant development did not increase root colonisation and did not stimulate the associated acetylene reduction. By using the ¹⁵N dilution method, the affect of inoculation with A. brasilense in terms of plant N was calculated as 0.067 mg N₂ fixed per plant, i.e., 3.3% of the N in the root and 1.6% in the plant shoot were of atmospheric origin. This ¹⁵N dilution was comparable to that seen in plants inoculated with non-N₂-fixing Psudomonas fluorescens.     

不同种植模式下紫色土养分流失影响因子研究

通过建立5个不同种植模式的径流小区，对每个小区径流泥沙中养分的含量进行研究．探讨机械组成、微团聚体含量、粘粒团聚度等因子对养分流失影响的程度，结果发现：15～30cm土层中〈0．01mm的粘粒含量与径流泥沙中全钾含量和速效钾含量有显著的相关性。0～15cm层中的〈0．01mm和〈0．001mm微团聚体含量都与径流泥沙中有机质、速效钾和全氮的含量有显著的相关性。同层中〈0．01mm粘粒团聚度与径流泥沙中全氮含量和速效钾含量有显著的相关性，而同层〈0．001mm粘粒团聚度与径流泥沙中全磷含量、速效钾含量和速效磷含量有显著的正相关。     

Arsenic-contaminated soils phytotoxicity studies with sunflower and sorghum

Background, Aim and Scope  Environmental pollution caused by arsenic (As) is a major ecological problem. There has been intense worldwide effort to find As-hyperaccumulating plants that can be used in phytoremediation—the green-plant-assisted removal of chemical pollutants from soils. For phytoremediation, it is natural to prefer cultivated rather than wild plants, because their agriculture is well known. This study was conducted to evaluate the tolerance of common sunflower(Helianthus annuus L.) and sugar sorghum(Sorghum saccharatum Pers.) for soil-As contents of 10–100 mg As kg^-1 soil, with sodium arsenite as a model contaminant. Methods  Plants were grown in a growth chamber for 30 days. Microfield experiments were conducted on experimental plots. To study the phytoremediation effect of the auxins indole-3-acetic acid (IAA) and 2,4-dichlorophenoxyacetic acid (2,4-D), we treated 1- and 3-day-old plant seedlings with water solutions of the auxins (concentrations 10^-5, 10^-7, and 10^-9 g l^-1). The soil and plant-biomass samples were analyzed for total As by using the color reaction of ammonium molybdate with As. Results and Discussion  Phytotoxicity studies showed that 100 mg As kg^-1 soil poisoned sunflower and sorghum growth by 50%. There was a linear correlation between soil-As content and As accumulation in the plants. Laboratory experiments showed that the soil-As content was reduced two- to threefold after sunflower had been grown with 10–100 mg As kg^-1 soil for 30 days. Treatment of sunflower and sorghum seedlings with IAA and 2,4-D at a concentration of 10^-5 g l^-1 in microfield experiments enhanced the phytoremediation two- to fivefold as compared with untreated control plants. The best results were obtained with 3-day-old seedlings. Conclusion, Recommendation and Outlook  (a) Sunflower and sorghum are good candidates to remediate As-polluted soils. (b) Phytoremediation can be improved with IAA or 2,4-D. (c) Mixed cropping of sorghum and sunflower may be another way of improving phytoremediation.