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排序方式: 共有689条查询结果,搜索用时 15 毫秒
21.
[目的]研究桐子叶水提物对小鼠的抗敏作用。[方法]用卵清蛋白作为致敏抗原,制作小鼠致敏模型,豌察桐子叶水提物对小鼠过敏反应的作用。[结果]高、中浓度组桐子叶水提物对卵清蛋白引起的小鼠过敏反应均有明显的抑制作用。[结论]桐子叶水提物对卵清蛋白致敏的小鼠有明显的抗敏作用。 相似文献
22.
Galvez J de Souza Gracioso J Camuesco D Galvez J Vilegas W Monteiro Souza Brito AR Zarzuelo A 《Fitoterapia》2006,77(7-8):515-520
Turnera ulmifolia is a plant popularly known in Brazil and South America as chanana. Some species of Turnera are widely used in folk medicine for different types of inflammatory diseases. In this study, the preventive intestinal antiinflammatory activity of a lyophilized infusion obtained from the aerial parts of T. ulmifolia was tested in the trinitrobenzenesulphonic acid (TNBS) model of rat colitis. The results obtained revealed that pretreatment to colitic rats with the extract, at 250 and 500 mg/kg, significantly attenuated the colonic damage induced by TNBS. This beneficial effect was associated with an improvement in the colonic oxidative status, since the infusion prevented the glutathione depletion that occurred as a consequence of the colonic inflammation. On the other hand, this antioxidant activity was confirmed in in vitro studies. In conclusion, the preventive effect exerted by the lyophilized infusion of T. ulmifolia in the TNBS model of rat colitis is probably related to its antioxidant properties, due to its flavonoids content. 相似文献
23.
[目的]探讨无公害灭鼠剂克鼠星对退耕还林地鼠害的防治效果。[方法]于2000~2004年在陕西省吴旗、甘泉、千阳、麟游、永寿和淳化6县,采用无公害灭鼠剂克鼠星对退耕还林地鼠害进行防治推广试验。[结果]在鼠害严重的造林地,造林前用克鼠星防治1次,造林后再用克鼠星防治1次,平均造林成活率达到97.1%,造林后2年平均保存率达到95.3%,分别比对照提高18.4%和28.5%。果树由于立地条件较好,鼠害防治彻底,2年保存率达到97.3%。应用灭鼠剂克鼠星1号进行鼢鼠防治,春季和秋季的防治效果均在89.4%以上,对达乌尔鼠兔的防治率均在84.7%以上。[结论]灭鼠剂克鼠星的研制与应用,杀灭了害鼠,同时保护了天敌,在农林牧业鼠害的防治方面,具有广阔的应用前景。 相似文献
24.
用毛细管区带电泳法(CZE)测定了小鼠脑中的铝,在10mmol/L苄胺和40%甲醇溶液中,Al3+在7min内与其他组分分离,用间接紫外吸收法在 214nm处检测,并以外标法定量,对样品进行5次测定,RDS为2.9%,回收率为96.8%,方法简便、快速、灵敏度高,重现性好,成本低。 相似文献
25.
Three consecutive days of injections of triiodothyronine (T3)(0.038, 0.075, 0.15 and 1.54 nmoles/g) significantly elevated the acetylcholinesterase (AchE) activity in the brain of Singi fish, Heteropneustes fossilis (Bloch). The higher doses of 0.075, 0.15 and 1.54 nmoles of T3/g induced a greater increase in enzyme activity than 0.038 nmoles/g. A T3 dose of 0.019 nmoles/g was found to be ineffective. The T3 action on AchE activity was blocked by cycloheximide. Thiourea treatment for 30 days decreased the AchE activity below the control level. This reduced level of the enzyme activity was brought back even above the control level by T3 injections. It is, therefore, suggested that thyroid hormone is involved in the sustenance of AchE activity in fish brain. 相似文献
26.
Hana Duranova Monika Martiniakova Radoslav Omelka Birgit Grosskopf Ivana Bobonova Robert Toman 《Acta veterinaria Scandinavica》2014,56(1)
Background
Chronic exposure to cadmium (Cd), even at low concentrations, has an adverse impact on the skeletal system. Histologically, primary and secondary osteons as basic structural elements of compact bone can also be affected by several toxicants leading to changes in bone vascularization and mechanical properties of the bone. The current study was designed to investigate the effect of subchronic peroral exposure to Cd on femoral bone structure including histomorphometry of the osteons in adult male rats.In our study, 20 one-month-old male Wistar rats were randomly divided into two experimental groups. In the first group, young males received a drinking water containing 30 mg of CdCl2/L, for 90 days. Ten one-month-old males without Cd intoxication served as a control group. After 90 days of daily peroral exposure, body weight, femoral weight, femoral length, cortical bone thickness and histological structure of the femora were analysed.Results
We found that subchronic peroral application of Cd had no significant effect on body weight, femoral length and cortical bone thickness in adult rats. On the other hand, femoral weight was significantly increased (P < 0.05) in Cd-intoxicated rats. These rats also displayed different microstructure in the middle part of the compact bone where vascular canals expanded into central area of substantia compacta and supplied primary and secondary osteons. Additionally, a few resorption lacunae which are connected with an early stage of osteoporosis were identified in these individuals. Histomorphometrical evaluations showed that all variables (area, perimeter, maximum and minimum diameter) of the primary osteons’ vascular canals, Haversian canals and secondary osteons were significantly decreased (P < 0.05) in the Cd group rats. This fact points to alterations in bone vascularization.Conclusions
Subchronic peroral exposure to Cd significantly influences femoral weight and histological structure of compact bone in adult male rats. It induces an early stage of osteoporosis and causes reduced bone vascularization. Histomorphometrical changes of primary and secondary osteons allow for the conclusion that the bone mechanical properties could be weakened in the Cd group rats. The current study significantly expands the knowledge on damaging action of Cd on the bone. 相似文献27.
K. Hylland C. Haux C. Hogstrand K. Sletten R. A. Andersen 《Fish physiology and biochemistry》1994,13(1):81-91
One isoform of the low-molecular-weight metal-binding protein metallothionein (MT) has been isolated from the liver of Atlantic
cod by size-exclusion and ion-exchange chromatography. Cod MT contained 33% cysteine, no aromatic amino acids or arginine.
As is the case for other piscine MTs, the N-terminus of cod MT lacked the asparagine in position 4 which is present in mammalian
MTs. In addition, cod MT differed from all other vertebrate MTs described in that the N-terminal methionine was not acetylated.
Antibodies were raised in rabbits against hepatic MT from cod by repeated injections of native protein mixed with adjuvant.
Anti-cod MT antisera cross reacted with similarly-sized proteins in liver, brain, anterior kidney, posterior kidney, spleen,
intestine, gills and ovaries. The putative MT in cod brain migrated differently to that of the other tissues in native gel
electrophoresis. Intraperitoneally injected Cd (1 mg/kg) was nearly entirely associated with the MT-peak in hepatic and renal
cytosols, whereas a single injection of Zn (10 mg/kg) resulted in increases in all cytosolic Zn pools of the liver and no
apparent change in cytosolic Zn, Cu, Ni or Cd in kidney. 相似文献
28.
29.
Three natriuretic peptides with similar structures were isolated from eels and their amino acid sequences determined; atrial
natriuretic peptide (ANP) from atria, ventricular natriuretic peptide (VNP) from ventricles, and C-type natriuretic peptide
(CNP) from brains. All three hormones were circulating in eel blood, and their plasma levels invariably decreased when eels
were transferred from fresh water (FW) to seawater (SW). Eel ANP and VNP inhibited drinking in FW and SW eels. Eel ANP inhibited
water and Na+ absorption by the intestine of SW eels. The potency of these ANP effects was 2–3 orders greater than those of other hormones
which are known to have similar effects. Eel ANP and VNP induced antidiuresis but not antinatriuresis in FW eels. Eel ANP
increased plasma cortisol level in SW eels but not in FW eels. The antidiuretic effect and the stimulation of cortisol secretion
in eels are opposite to the ANP effects reported in mammals. These data suggest that ANP plays a complex role in the eel osmoregulation.
Résumé Trois peptides natriurétiques présentant des structures similaires ont été isolés chez l'anguille et leurs séquences en acides aminés a été déterminées; le peptide atrial natriurétique (ANP) dans l'atria, le peptide ventriculaire natriurétique (VNP) dans les ventricules et le peptide natriurétique de type C dans le cerveau. Ces trois hormones sont présents dans le sang d'anguille et leur niveau plasmatique decroit invariablement quand les anguilles sont transférées d'eau douce en eau de mer. L'ANP et le VNP d'anguille inhibent l'action de boire chez les anguilles d'eau douce et d'eau de mer. L'ANP d'anguille inhibe l'absorption d'eau et de Na+ par l'intestin d'anguille en eau de mer. Ces effets de l'ANP sont 2 à 3 fois plus grands que ceux observés avec d'autres hormones qui sont connues pour avoir des effets similaires. L'ANP et le VNP d'anguille induisent l'antidiurèse mais pas l'antinatriurèse chez les anguilles d'eau douce. L'ANP d'anguille augmente chez ce poisson les niveaux de cortisol plasmatique en eau de mer mais pas en eau douce. L'effet antidiurétique et la stimulation de la sécrétion de cortisol chez l'anguille sont contraire aux effets de l'ANP observés chez les mammifères. Ces résultats suggèrent que l'ANP joue un role complexe dans l'osmorégulation de l'anguille.相似文献
30.