A rapid, seedling-based bioassay for identifying low cadmium-accumulating individuals of Durum wheat (Triticum turgidum L.).

We have developed a seedling-based bioassay that iscapable of identifying low Cd-accumulating phenotypes(homozygous and heterozygous) after 96–120 h ofexposure. Our experiments were conducted using¹⁰⁹Cd as a tracer at subtoxic concentrations tosimulate conditions that might be experienced in thefield. Supply of Cd (10^-11 M) to 4-d oldseedlings for 0–8 h resulted in no differences in rootand leaf Cd content between the low (TL05) and high(TL04) Cd-accumulating isolines. Increasing time ofexposure produced significant differences in leaf Cdaccumulation between isolines, with differencesbecoming most pronounced after the bulk of appliedcadmium ( 95%) was depleted from absorption solutions( 72 h). Similar results were obtained with8-d old seedlings, where differences between genotypeswere more pronounced in young leaves (2nd leaf) orshoot bases. Individuals from five low and highCd-accumulating near isogenic pairs (50individuals/isoline) were screened using Cdconcentration of shoot bases as the screeningcriterion. Mean scores within each isoline pair weresignificantly different, although overlap ofindividual scores was observed at intermediate foliarCd concentrations. The 2^nd leaf to root Cdcontent ratio, which reflects root to shoottranslocation, provided a better parameter todistinguish low from high Cd-accumulating isolines. Plants used for this bioassay could also be rescuedfor subsequent experimental crosses, providing a rapidand cost-effective tool for early detection of the lowCd-accumulating phenotype.     

Effect of Drechslera (Helminthosporium) sacchari Toxin upon the Permeability of Sugarcane Leaf and Callus Tissues

Sugarcane leaves and calli from highly susceptible and resistant varieties to eyespot disease were used to evaluate the Drechslera sacchari toxin effect at different concentrations and incubation times by measuring electrolyte leakage. This expression of disease resistance was observed not only in the leaf but also in the callus. Furthermore, the growth of resistant calli MS medium supplemented with DS toxin, was higher when compared to the susceptible ones. The possibility of obtaining resistant somaclones is confirmed.     

FEM Dynamical Analysis of the Reinforced Concrete Beam with Bonding Steel Plate

In order to researchmechanical capability of the reinforced concrete beam with bonding steel plate, adopting separatemethod to establish finite element model of the reinforced concrete beam with bonding steel plate, using FEM to study dynamical characteristics of the model, have got good results which can not be easily got using traditional resolution method, which can give referenceto study dynamical characteristics of the reinforced concrete beam with bonding steel plate.     

粘虫痘病毒增效蛋白的重组表达及其生物活性

昆虫病毒增效蛋白(enhancin)在体外能促进核型多角体病毒(NPV)对昆虫离体细胞系的感染;在体内能显著提高NPV对昆虫幼虫的感染效果,并可增强苏云金芽孢杆菌(Bt)对幼虫的毒力(Hashimoto et al.,1991;Roelvink et al.,1995)。粘虫痘病毒(Pseudaletia separata entomopoxvirus,PsEP     

微热管阵列平板太阳能集热器中空保温层厚度优化

为分析微热管阵列平板太阳能集热器的热性能,该文建立了集热器的CFD模型,对其进行数值模拟,将模拟结果与试验结果进行对比,验证了模型的可靠性。采用该CFD模型对集热器保温层厚度进行优化,结果表明,当实心保温层导热系数分别为0.02、0.03、0.04、0.05W/(m·K)时,优化的实心保温层厚度分别为4.5、5.0、5.5、5.5cm。合理设计的中空保温层(空气层与实心保温层相结合的保温层形式)集热器能够达到与实心保温层集热器相当的保温隔热效果,同时可使集热器保温层成本及质量降低25%~50%。最后,该文给出了保温层总厚度分别为4、5、6cm时的中空保温层厚度优化结果,为该类集热器保温层的设计提供了理论依据。     

板齿摘脱滚筒的核心功能——吸附作用的理论研究

从观察现象出发，经理论分析旋转气流所产生的使茎秆、穗头向滚筒贴附的作用，揭示了该作用与板齿结构和运动参数间的关系，并对环流流场的流速、压头的形成亦进行了分析。实验测定结果，基本验证了这些关系     

Effect of subcritical hydrophobicity in a sandy soil on water infiltration and mobile water content

Recent research shows that most soils are more or less water repellent. Already subcritical water repellency may cause incomplete soil wetting and preferential flow. Both processes potentially reduce the residence time of water and solutes in the vadose zone, resulting in an enhanced risk of groundwater contamination. The objective of the present paper is, therefore, to evaluate the impact of reduced soil wettability on the soil water infiltration rate and to investigate the tendency towards preferential flow with the analysis of the immobile water content in the infiltration zone. In november 2002, a field experiment was done in a coniferous forest, 30 km N of Hannover, Germany. Soil hydrophobicity was quantified by measuring the contact angles. The hydraulic conductivity of the podsolic sandy soil was measured depth‐dependent with a double‐ring tension infiltrometer in three soil horizons. To quantify possible preferential‐flow effects, a LiBr‐Tracer was added to the infiltrating water to evaluate the mobile water‐content fraction after infiltration. Additionally, infiltration rates of water were compared with infiltration rates of ethanol which were determined after water infiltration at the same locations. Results show that the actual water repellency of field‐moist soil was mainly subcritical (contact angle <90°). Water infiltration rates were reduced due to subcritical repellency by a factor of 3–170 compared with ethanol infiltration rates (exclusion of wetting effects). This spatially variable infiltration behavior was not clearly reflected neither by the small‐scale contact‐angle measurements nor by the analysis of the average immobile soil water content in the infiltration zone. We conclude that this specific infiltration behavior of water caused by small‐scale wettability effects may temporarily reduce the local connectivity of water‐flow pathways.     

Mechanical Model of Steel Plate Composite Shear Walls

Based on concepts of mechanics, a mechanical model of novel type of steel plate composite shear wall is presented. The novel type of structure is formed by steel plate shear wall and T-shaped solid-web composite columns. Flexural stiffness of steel beams, lateral stiffness of the T-shaped solid-web composite columns, shear stiffness of steel plate composite shear wall and shear stiffness of beam-column connection are taken into account in the mechanical model. And the equivalent damping between steel plate and boundary is considered. Based on the deformation features of structures and the calculation hypothesis, the lateral stiffness model and the energy dissipation model of structures are developed. Meanwhile, the calculation equations of elastic ultimate and plastic ultimate of shear strength of structures are set up. The theoretical analysis results inosculate better with the results of experiment. The comparison between the result calculated from the formula and the experimental result shows that the calculation precision is high enough to meet the demand of theoretical analysis. The difference, including equivalent model, stress states, manufacturing defect and installation error, between the formula and the experiment has been further discussed.     

苜蓿提取液抑菌活性研究

[目的]研究苜蓿提取液对4种常见菌绿脓杆菌、停乳链球菌、大肠杆菌和金黄色葡萄球菌的抑菌活性。[方法]采用二倍稀释法和血平板鉴定相结合的方法测定其最低抑菌浓度(MIC)。[结果]苜蓿提取液对绿脓杆菌和停乳链球菌的MIC分别为0.674和1.347mg/ml;对大肠杆菌和金黄色葡萄球菌的MIC均为2.695 mg/ml。[结论]苜蓿提取液对绿脓杆菌和停乳链球菌有较好的抑菌活性,对大肠杆菌和金黄色葡萄球菌的抑菌效果一般。     

包申格效应对X65管道钢管强度的影响

进口X65卷板制管后屈服强度明显下降，通过包申格效应试验，分析制管过程中的应力，应变对钢管屈服强度的影响，对进口卷板制管前后屈服强度的变化情况进行了详细研究，指出了影响钢管强度的几个主要因素。