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排序方式: 共有123条查询结果,搜索用时 31 毫秒
101.
Ying Wang Xiao-Ming Li Sui-Qun Yang Fan-Zhong Zhang Bin-Gui Wang Hong-Lei Li Ling-Hong Meng 《Marine drugs》2022,20(3)
An unusual sesquiterpene glycoside trichoacorside A (1) and two novel sorbicillinoid glycosides sorbicillisides A (2) and B (3), together with a known compound sorbicillin (4), were isolated and identified from the culture extract of an endophytic fungus Trichoderma longibrachiatum EN-586, obtained from the marine red alga Laurencia obtusa. Trichoacorside A (1) is the first representative of a glucosamine-coupled acorane-type sesquiterpenoid. Their structures were elucidated based on detailed interpretation of NMR and mass spectroscopic data. The absolute configurations were determined by X-ray crystallographic analysis, chemical derivatization, and DP4+ probability analysis. The antimicrobial activities of compounds 1–4 against several human, aquatic, and plant pathogens were evaluated. 相似文献
102.
In this study, the influence of changes in fruit lighting on individual and total phenols in ‘Fuji’ apple, as well as color development was studied. Content levels of eight quercetin glycosides, five anthocyanins, two catechins and a hydroxycinnamic acid in the skin of apples were analyzed, using high-performance liquid chromatography, tandem mass spectrometry (HPLC-MS). Total phenol, chlorophyll and carotenoid contents were determined by spectrometry. The purpose of this study was to compare content levels of those compounds in apple skin of fruit grown in different parts of the tree canopy, under and outside of the hail net. Lighting of fruit was measured during the last month before harvest. The lowest values were measured in the inner fruit and higher ones in the outer parts of the canopy, while the highest values were measured in fruit growing at the top of the tree. The hail net had no influence on the decrease of lighting in comparison to the control. Light conditions in the tree canopy influenced lower content levels of quercetin glycosides and most anthocyanins in the fruit skin in the inner part of the tree canopy, whereas fruit from the canopy top contained the highest levels of quercetin glycosides and cyanidin glycosides. Catechin, epicatechin and chlorogenic acid content levels in apple skin were independent of fruit position in the canopy or hail net usage, except for chlorogenic acid, where the content level was higher in cases when the orchard was covered with a hail net. Fruit from the top and outer parts of the canopy had a darker and redder coloration than inner fruit, while no influence of canopy position on chlorophyll and carotenoids was detected. Since quercetin glycosides and cyanidin glycosides are influential in red skin color development, better coloration of fruit from the outer and top canopy was observed. More intensive lighting stimulated a higher content level of flavonoids and, consequently, better coloration, which is an important factor in fruit quality. 相似文献
103.
Youngdeuk Lee Eunyoung Jo Yeon-Ju Lee Tae-Yang Eom Yehui Gang Yoon-Hyeok Kang Svini Dileepa Marasinghe Sachithra Amarin Hettiarachchi Do-Hyung Kang Chulhong Oh 《Marine drugs》2022,20(1)
We recently identified a β-agarase, Gaa16B, in the marine bacterium Gilvimarinus agarilyticus JEA5. Gaa16B, belonging to the glycoside hydrolase 16 family of β-agarases, shows less than 70.9% amino acid similarity with previously characterized agarases. Recombinant Gaa16B lacking the carbohydrate-binding region (rGaa16Bc) was overexpressed in Escherichia coli and purified. Activity assays revealed the optimal temperature and pH of rGaa16Bc to be 55 °C and pH 6–7, respectively, and the protein was highly stable at 55 °C for 90 min. Additionally, rGaa16Bc activity was strongly enhanced (2.3-fold) in the presence of 2.5 mM MnCl2. The Km and Vmax of rGaa16Bc for agarose were 6.4 mg/mL and 953 U/mg, respectively. Thin-layer chromatography analysis revealed that rGaa16Bc can hydrolyze agarose into neoagarotetraose and neoagarobiose. Partial hydrolysis products (PHPs) of rGaa16Bc had an average molecular weight of 88–102 kDa and exhibited > 60% hyaluronidase inhibition activity at a concentration of 1 mg/mL, whereas the completely hydrolyzed product (CHP) showed no hyaluronidase at the same concentration. The biochemical properties of Gaa16B suggest that it could be useful for producing functional neoagaro-oligosaccharides. Additionally, the PHP of rGaa16Bc may be useful in promoting its utilization, which is limited due to the gel strength of agar. 相似文献
104.
Organisms specialized to thrive in cold environments (so-called psychrophiles) produce enzymes with the remarkable ability to catalyze chemical reactions at low temperature. Cold activity relies on adaptive changes in the proteins’ sequence and structural organization that result in high conformational flexibility. As a consequence of flexibility, several such enzymes are inherently heat sensitive. Cold-active enzymes are of interest for application in a number of bioprocesses, where cold activity coupled with easy thermal inactivation can be of advantage. We describe the biochemical and functional properties of two glycosyl hydrolases (named LYS177 and LYS188) of family 19 (GH19), identified in the genome of an Antarctic marine Pseudomonas. Molecular evolutionary analysis placed them in a group of characterized GH19 endolysins active on lysozyme substrates, such as peptidoglycan. Enzyme activity peaks at about 25–35 °C and 40% residual activity is retained at 5 °C. LYS177 and LYS188 are thermolabile, with Tm of 52 and 45 °C and half-lives of 48 and 12 h at 37 °C, respectively. Bioinformatics analyses suggest that low heat stability may be associated to temperature-driven increases in local flexibility occurring mainly in a specific region of the polypeptide that is predicted to contain hot spots for aggregation. 相似文献
105.
106.
Irina Bakunina Olga Nedashkovskaya Larissa Balabanova Tatyana Zvyagintseva Valery Rasskasov Valery Mikhailov 《Marine drugs》2013,11(6):1977-1998
A total of 16 marine strains belonging to the genus Arenibacter, recovered from diverse microbial communities associated with various marine habitats and collected from different locations, were evaluated in degradation of natural polysaccharides and chromogenic glycosides. Most strains were affiliated with five recognized species, and some presented three new species within the genus Arenibacter. No strains contained enzymes depolymerizing polysaccharides, but synthesized a wide spectrum of glycosidases. Highly active β-N-acetylglucosaminidases and α-N-acetylgalactosaminidases were the main glycosidases for all Arenibacter. The genes, encoding two new members of glycoside hydrolyses (GH) families, 20 and 109, were isolated and characterized from the genomes of Arenibacter latericius. Molecular genetic analysis using glycosidase-specific primers shows the absence of GH27 and GH36 genes. A sequence comparison with functionally-characterized GH20 and GH109 enzymes shows that both sequences are closest to the enzymes of chitinolytic bacteria Vibrio furnissii and Cellulomonas fimi of marine and terrestrial origin, as well as human pathogen Elisabethkingia meningoseptica and simbionts Akkermansia muciniphila, gut and non-gut Bacteroides, respectively. These results revealed that the genus Arenibacter is a highly taxonomic diverse group of microorganisms, which can participate in degradation of natural polymers in marine environments depending on their niche and habitat adaptations. They are new prospective candidates for biotechnological applications due to their production of unique glycosidases. 相似文献
107.
研究了不同浓度萘乙酸(NAA)对黄瓜根、芽生长的影响。结果表明,不同浓度萘乙酸对黄瓜根、芽的生长影响不同,与对照相比,萘乙酸在0.0001~0.01mg/L浓度范围内促进黄瓜根、芽生长,最佳浓度为0.0001mg/L。当浓度超过1mg/L时,萘乙酸则明显抑制黄瓜根、芽生长,当浓度为10mg/L时,黄瓜幼苗出现烂根现象。 相似文献
108.
In this study the fate of naphthalene, fluorene and pyrene were investigated in the presence and absence of enchytraeid worms. Microcosms were used, which enabled the full fate of 14C-labelled PAHs to be followed. Between 60 and 70% of naphthalene was either mineralised or volatilised, whereas over 90% of the fluorene and pyrene was retained within the soil. Mineralisation and volatilisation of naphthalene was lower in the presence of enchytraeid worms. The hypothesis that microbial mineralisation of naphthalene was limited by enchytraeids because they reduce nutrient availability, and hence limit microbial carbon turnover in these nutrient poor soils, was tested. Ammonia concentrations increased and phosphorus concentrations decreased in all microcosms over the 56 d experimental period. The soil nutrient chemistry was only altered slightly by enchytraeid worms, and did not appear to be the cause of retardation of naphthalene mineralisation. The results suggest that microbial availability and volatilisation of naphthalene is altered as it passes through enchytraeid worms due to organic material encapsulation. 相似文献
109.
Three new phenolic glycosides, curculigosides F–H (1–3), were isolated from rhizomes of Curculigo orchioides Gaertn. Their structures were elucidated based on comprehensive spectroscopic analyses including IR, MS, 1D- and 2D NMR (HSQC, COSY, and HMBC). Curculigosides F–H (1–3) were evaluated for their anti-HBV activity in vitro using the HBV transfected Hep G2.2.15 cell line. Compound 1 exhibited weak activity with an IC50 value of 2.08 mM on hepatitis B virus (HBV) e antigen (HBeAg) secretion of the HepG2.2.15 cell line. 相似文献
110.
A new triglucosylated naphthalene derivative, named aloveroside A (1), together with two known anthraquinone dimers and two 6-phenyl-2-pyrone derivatives, was isolated from the Aloe vera ethanolic extracts. The structure of 1 was established as 1-(((4-(1-O-β-D-glucopyranosyl -(1→4)-β-D-xylopyranoside)-hydroxymethyl)-1-hydroxy-8-O-α-L-rhamnopyranoside)naphthalene-2-yl)-ethanone by means of spectroscopic evidences and chemical methods. All these compounds were tested for their BACE inhibitory activity but no significant activities were found. 相似文献