全文获取类型
收费全文 | 22300篇 |
免费 | 935篇 |
国内免费 | 2254篇 |
专业分类
林业 | 1078篇 |
农学 | 1868篇 |
基础科学 | 385篇 |
4935篇 | |
综合类 | 7883篇 |
农作物 | 1485篇 |
水产渔业 | 807篇 |
畜牧兽医 | 4689篇 |
园艺 | 1468篇 |
植物保护 | 891篇 |
出版年
2024年 | 89篇 |
2023年 | 327篇 |
2022年 | 576篇 |
2021年 | 769篇 |
2020年 | 750篇 |
2019年 | 856篇 |
2018年 | 599篇 |
2017年 | 941篇 |
2016年 | 1166篇 |
2015年 | 1036篇 |
2014年 | 1153篇 |
2013年 | 1525篇 |
2012年 | 1707篇 |
2011年 | 1725篇 |
2010年 | 1505篇 |
2009年 | 1505篇 |
2008年 | 1268篇 |
2007年 | 1456篇 |
2006年 | 1223篇 |
2005年 | 882篇 |
2004年 | 711篇 |
2003年 | 578篇 |
2002年 | 420篇 |
2001年 | 345篇 |
2000年 | 361篇 |
1999年 | 289篇 |
1998年 | 201篇 |
1997年 | 214篇 |
1996年 | 218篇 |
1995年 | 172篇 |
1994年 | 126篇 |
1993年 | 142篇 |
1992年 | 131篇 |
1991年 | 103篇 |
1990年 | 92篇 |
1989年 | 80篇 |
1988年 | 63篇 |
1987年 | 46篇 |
1986年 | 37篇 |
1985年 | 27篇 |
1984年 | 16篇 |
1983年 | 10篇 |
1982年 | 5篇 |
1981年 | 7篇 |
1980年 | 5篇 |
1978年 | 5篇 |
1977年 | 4篇 |
1962年 | 4篇 |
1956年 | 9篇 |
1955年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
D. Bilalis P. Efthimiadis & G. Karagiannis 《Journal of Agronomy and Crop Science》2001,187(2):121-126
The effect of three herbicides, diclofop-methyl (DM), imazamethabenz-methyl (IM) and fenoxaprop-ethyl (FE), was investigated at the recommended rate (×) and double the recommended rate (2×) in a durum wheat crop. FE had the smallest effect on root system characteristics, while IM had the greatest effect. For all herbicides, the double rate treatment gave a greater statistical reduction than the single rate for root system characteristics. FE had the smallest effect on the activity of aerobic microorganisms, while DM gave the greatest reduction, 10 days after application, and all three herbicides reduced earthworm abundance. DM gave the smallest reduction in the number of tillers and in plant height, while the double rate of IM gave the greatest reduction. For all three herbicides, double the recommended rate resulted in a significant reduction in the weight of 1000 seeds, while no statistically significant deviations were noted at the recommended rate. Finally, the use of all three herbicides resulted in significantly lower statistical yields compared to the sample (control plot), although there were no significant statistical differences among them, for either recommended or double recommended rates. 相似文献
2.
J. Müller 《Journal of Agronomy and Crop Science》1993,171(4):217-235
Dry Matter Production, CO2 Exchange, Carbohydrate and Nitrogen Content of Winter Wheat at Elevated CO2 Concentration and Drought Stress
Methods of mathematical modelling and simulation are being used to an increasing degree in estimating the effects of rising atmospheric CO2 concentration and changing climatic conditions on agricultural ecosystems. In this context, detailed knowledge is required about the possible effects on crop growth and physiological processes. To this aim, the influence of an elevated CO2 concentration and of drought stress on dry matter production, CO2 exchange, and on carbohydrate and nitrogen content was studied in two winter wheat varieties from shooting to milk ripeness. Elevated CO2 concentration leads to a compensation of drought stress and at optimal water supply to an increase of vegetative dry matter and of yield to the fourfold value. This effects were caused by enhanced growth of secondary tillers which were reduced in plants cultivated at atmospheric CO2 concentration. Analogous effects in the development of ear organs were influenced additionally by competitive interactions between the developing organs. The content and the mass of ethanol soluble carbohydrates in leaves and stems were increased after the CO2 treatment and exhausted more completely during the grain filling period after drought stress. Plants cultivated from shooting to milk ripeness at elevated CO2 concentration showed a reduced response of net photosynthesis rate to increasing CO2 concentration by comparison with untreated plants. The rate of dark respiration was increased in this plants. 相似文献
Methods of mathematical modelling and simulation are being used to an increasing degree in estimating the effects of rising atmospheric CO
3.
In this study, the effects of nitrogen (N) rate (60, 120, 180 and 240 kg N ha?1 applied in three equal dressings at seeding and after the first and second cuttings) and stubble height (7, 14 and 21 cm) on the dry matter (DM) yield, crude protein (CP) content, and CP yield of a sorghum–sudangrass hybrid [Sorghum bicolor (L.) Moench × Sorghum sudanense (Piper) Stapf., cv. Pioneer 988] in the three‐cut system was investigated. The N rate had no significant effect in the first and third cuttings, but in the second cutting DM yields increased significantly with increase in N rate. The highest yield of 9.1 t ha?1 was obtained with 80 kg N ha?1 for the average of 2 years at the second cutting, but no significant difference was found among the 40, 60 and 80 kg N ha?1 rates. CP content and yield were not significantly affected by N rate at the first and third cuttings, but CP content and yield were significantly affected by application of N at the second cutting. Stubble height had a significant effect on CP content at the third cutting. However, it had no significant effect on CP content at the first and second cuttings. Stubble height had a significant effect on the CP yield at the first cutting, but no significant effect on CP yield at the second and third cuttings. 相似文献
4.
Field experiments were conducted at Tamil Nadu Rice Research Institute, Aduthurai, India, during the wet seasons of 1992 and 1993 to study the effect of full and partial substitution of fertiliser N with green manure N (Sesbania rostrata) on nitrogen uptake, yield attributes and yield of rice. The experiment consisted of eight treatments with two levels of N (100 and 200 kg ha−1 ) and three sources of N application viz., fertilizer, integrated (1:1 fertilizer and green manure N) and green manure N compared to the recommended practice (150 kg fertilizer plus 6.25 t ha−1 (72 kg N) green manure) and a no N control. Nitrogen application markedly increased the N uptake. Combined use of the two N sources at 200 and 222 kg N ha−1 and of single fertilizer N at 200 kg N ha−1 recorded the maximum N uptake, increased the yield attributes such as number of panicles per unit area, weight per panicle, number of total and filled grains per panicle and test weight. At 200 kg N ha−1 full substitution of N by green manure reduced the grain yield but only partial substitution of N by green manure resulted in almost similar yield as single fertilizer N. Thus 200 kg N ha−1 applied in equal proportions of fertilizer and green manure N can be recommended for medium duration rice cultivars. 相似文献
5.
Two-year field trials with winter wheat cultivars Batis and Toronto were conducted in Southern Bavaria, Germany, to investigate the possible causes of cultivar differences in response to N supply varying in total amount and time of application. Cultivar-related differences in grain yields were observed in treatments with low and medium N supply. High doses of N supply resulted in grain yield adjustment or grain yield advantage for cv. Toronto. The results of this study revealed a consistent, genotypic pattern in response to N fertilization in spite of strong seasonal effects. Systematic modifications in canopy growth rates in response to N supply were of particular relevance and a main factor for differences in tillering intensity resulting in modified stand densities. In the present study, cultivar differences in spike development and interactions with N supply related more to abortion than to initiation processes for number of spikelets and number of flowers per spikelet. High grain density (grains per m2) of cv. Toronto was evident during reproduction stages even under conditions of medium N supply. However, decreased growth rates during the later part of grain filling in combination with low 1000 grain weight, which was barely modified by N fertilization, allowed only partial utilization of this potential. It is assumed that sink limitations were of particular relevance for grain yield development in cv. Toronto, while cv. Batis combined a less intense response to N supply with more stability in the development of grain yield components. 相似文献
6.
INTRODUCTIONIntheworldoftoday,theglobalclimatechangeanditsinfluenceonecologyhavebe-comeaveryimportantproblem,towhichmanyscientists,governnentleadersandordinarypcoplepaycloseattentionI1-'].Inl979,theWorldClimateResearchProgram(WCP)waslaiddowninthefirstworldclimatemeeting.lnl99(),thesecondworldclimatemeetingwasconvcl1edinGencva,andalltl1eexpertsagreedthattheglobalwarmlngwillbeextremelyseriousdisasterthananynatUralcalamityever.Attl1eMectingof"WorldEnvironmentandDevelopment,"holdinBrazil… 相似文献
7.
Nir Melnikov Yehuda Kamari Michal Kandel-Kfir Iris Barshack Ami Ben-Amotz Dror Harats Aviv Shaish Ayelet Harari 《Marine drugs》2022,20(7)
Vitamin A and provitamin A carotenoids are involved in the regulation of adipose tissue metabolism and inflammation. We examined the effect of dietary supplementation using all-trans and 9-cis β-carotene-rich Dunaliella bardawil alga as the sole source of vitamin A on obesity-associated comorbidities and adipose tissue dysfunction in a diet-induced obesity mouse model. Three-week-old male mice (C57BL/6) were randomly allocated into two groups and fed a high-fat, vitamin A-deficient diet supplemented with either vitamin A (HFD) or β-carotene (BC) (HFD-BC). Vitamin A levels in the liver, WATs, and BAT of the HFD-BC group were 1.5–2.4-fold higher than of the HFD group. BC concentrations were 5–6-fold greater in BAT compared to WAT in the HFD-BC group. The eWAT mRNA levels of the Mcp-1 and Cd68 were 1.6- and 2.1-fold lower, respectively, and the plasma cholesterol and triglyceride concentrations were 30% and 28% lower in the HFD-BC group compared with the HFD group. Dietary BC can be the exclusive vitamin A source in mice fed a high-fat diet, as shown by the vitamin A concentration in the plasma and tissues. Feeding BC rather than vitamin A reduces adipose tissue macrophage recruitment markers and plasma lipid concentrations. 相似文献
8.
规模猪场4大疫病的血清学检测及免疫抑制病因分析 总被引:4,自引:0,他引:4
选取杭州、绍兴、嘉兴和湖州4个地区的规模猪场为试验猪场,用正向间接血凝试验(IHA)检测猪瘟病毒(CSFV)和口蹄疫病毒(FMDV)抗体合格率;用酶联免疫吸附试验(ELISA)检测猪繁殖与呼吸综合征病毒(PRRSV)和猪圆环病毒2型(PCV2)抗体阳性率,分析免疫抑制状况。同时,采取临床可疑病料,用RT-PCR技术检测PRRSV、PCV2、CSFV和伪狂犬病毒(PRV)的感染率。结果显示:不同年龄猪群中,PCV2抗体阳性率都在96.0%以上,而CSFV、FMDV抗体合格率及PRRSV抗体阳性率呈现一致的变化趋势,即母猪最高,哺乳仔猪其次,培育猪和肥育猪最低;在病原检测中,PRRSV和PCV2检出率最高,分别为44.3%和60.7%。另外,双重感染和多重感染明显,特别是PRRSV+PCV2+CSFV三重感染,阳性率达7.6%。本试验结果为制定有效的免疫程序等防治措施提供科学依据。 相似文献
9.
10.
Kaiping Deng Steffen Uhlig Laura B. Goodman Hon S. Ip Mary Lea Killian Sarah M. Nemser Jodie Ulaszek Shannon Kiener Matthew Kmet Kirstin Frost Karina Hettwer Bertrand Colson Kapil Nichani Anja Schlierf Andriy Tkachenko Mothomang Mlalazi-Oyinloye Andrew Scott Ravinder Reddy Gregory H. Tyson 《Journal of veterinary diagnostic investigation》2022,34(5):825
The COVID-19 pandemic presents a continued public health challenge. Veterinary diagnostic laboratories in the United States use RT-rtPCR for animal testing, and many laboratories are certified for testing human samples; hence, ensuring that laboratories have sensitive and specific SARS-CoV2 testing methods is a critical component of the pandemic response. In 2020, the FDA Veterinary Laboratory Investigation and Response Network (Vet-LIRN) led an interlaboratory comparison (ILC1) to help laboratories evaluate their existing RT-rtPCR methods for detecting SARS-CoV2. All participating laboratories were able to detect the viral RNA spiked in buffer and PrimeStore molecular transport medium (MTM). With ILC2, Vet-LIRN extended ILC1 by evaluating analytical sensitivity and specificity of the methods used by participating laboratories to detect 3 SARS-CoV2 variants (B.1; B.1.1.7 [Alpha]; B.1.351 [Beta]) at various copy levels. We analyzed 57 sets of results from 45 laboratories qualitatively and quantitatively according to the principles of ISO 16140-2:2016. More than 95% of analysts detected the SARS-CoV2 RNA in MTM at ≥500 copies for all 3 variants. In addition, for nucleocapsid markers N1 and N2, 81% and 92% of the analysts detected ≤20 copies in the assays, respectively. The analytical specificity of the evaluated methods was >99%. Participating laboratories were able to assess their current method performance, identify possible limitations, and recognize method strengths as part of a continuous learning environment to support the critical need for the reliable diagnosis of COVID-19 in potentially infected animals and humans. 相似文献