Protein quality of peas as influenced by location,nitrogen application and seed inoculation

A study was conducted to evaluate the contribution of location, N application andRhizobium seed inoculation to variations in seed protein content and amino acid (AA) composition of field peas. The magnitude of AA variations with protein level and the nature of the relationships that are involved were determined. Regression equations to predict AAs from protein were developed for the cultivar Bohatyr. The experiments were carried out at two locations in southern Manitoba in 1994. The levels of N fertilization investigated were: 56, 75, 100, 125, 150, 200, 250 and 300 kg/ha. At each level of N application, seeds planted were eitherRhizobium inoculated or not inoculated. The combination of location, fertilizer treatments and inoculation yielded 192 samples for chemical analyses. The samples were analyzed for dry matter (DM), N and AA contents. Location and N fertilization had significant (p0.001) effects on seed protein content and AA composition. Seed protein content increased with increasing levels of N application. The response of protein to fertilization was not the same in both locations as evidenced from the presence of interaction (p0.01) between location and N application. Except for methionine and cystine, percent AAs in DM increased with increasing levels of N application. The effects of N application on the concentrations of methionine and cystine were not consistent. On protein basis, the concentrations of AAs decreased with increasing levels of N application. The only exception was arginine which strongly increased in concentration. There was no effect (p0.05) of seed inoculation observed in this study. Strong positive correlations (r>0.80) between seed protein content and AA concentrations expressed as percent of DM were found for all AAs except for methionine (r=0.76) and cystine (r=0.51). When AA concentrations are expressed as g per 16 g N, 15 of the 17 AAs were negatively correlated to seed protein content. Only arginine (r=0.78) and aspartic acid (r=0.17) had positive correlations. The regression equations developed from this study could be used to predict the concentrations of AAs except methionine and cystine for the cultivar Bohatyr once the protein content is known.     

河北省蔬菜业区域布局及发展对策刍议

阐述了河北省蔬菜产业规模扩大、质量提高的发展现状、适宜发展蔬菜业的资源、区位优势和制约因素,按照构建优势农产品产业带的要求,将河北蔬菜产区划分成冀中南、环京津、沿海、冀西北4个优势区域,分析了各区发展的方向、目标、重点及应采取的主要措施,提出了适当增加投入比重、采取优惠财政政策、开辟绿色通道等推进优势区域布局的政策性建议。     

滇1型杂交水稻质核雄性不育恢复基因的研究

用混合品系分析方法(BLA)，在滇1型杂交水稻的保持系和恢复系中的DNA各取20个样混合，对OSR—33引物进行筛选，结果发现OSR—33引物在保持系和恢复系中有遗传差异。然后又用这对引物分别对保持系和恢复系的单株DNA样品进行扩增。另外，用滇1型杂交粳稻的保持系和恢复系与不育系进行测交，杂种F1花粉的育性经1％的I—KI染色。用扩增出的带型与F1花粉的育性进行相关分析，结果表明扩增出带型与花粉的育性呈显著正相关，两种带型对应的花粉可育率均值经t测验差异极显著，表明OSR—33标记与滇1型细胞质雄性不育育性恢复基因连锁，由于OSR—33引物是设计在第10染色体长臂的中部，所以，滇1型细胞质雄性不育育性恢复的一个基因可初步定位于第10染色体长臂的中部。     

犬瘟热脏器组织中的抗原定位及病理形态学观察

本文采用免疫组化间接ＢＡ法，对临诊疑似犬瘟热病犬脏器组织中的抗原，进行了定位检查并进行了系统病理学观察。结果表明：发病犬大脑、小脑、延髓、肺、心、脾、膀胱、肠、肝、胰、肾上腺等组织细胞及血管内皮、支气管上皮细胞胞浆内均可见抗原阳性反应。同时，抗原阳性反应组织、器官均可见不同程度的病理变化。     

江苏地区雷电活动分布特征

利用江苏省气象部门提供的ADTD雷电探测系统2007年全年的闪电资料,分析了江苏地区闪电的月变化、日变化、季节变化等特征,并将所得结果与美国俄克拉荷马和堪萨斯州的闪电气候进行了对比。结果表明：江苏省云地闪中负闪占绝大多数。占总地闪的95．4％;地闪主要出现在夏季,占全年的92．8％,其中,8月份出现的地闪次数为全年之最,高达385817次,约占全年的45．7％;正地闪所占比例的月际变化与闪电次数正好相反,夏季正地闪所占的比例最小,而冬季正地闪所占比例较大;闪电的发生也有明显的日变化;雷电发生的主要时间段在各个季节是不同的,形成雷电的原因也不同。     

Determining soil enzyme location and related kinetics using rapid fumigation and high-yield extraction

Knowledge concerning the location of soil enzymes and their kinetics is necessary for a better understanding of their roles in biogeochemical cycles. A rapid dichloromethane fumigation procedure and a new extraction method giving negligible co-extraction of humic substances were used. Fumigation and consequent cell lysis increased the activity of arylsulfatase, but not that of acid and alkaline phosphomonoesterase, suggesting that only the former enzyme was located both extracellularly and intracellularly. Extracted arylsulfatase displayed a Michaelis–Menten constant (K_m) significantly lower than in fumigated soil, which in turn was significantly lower than in unfumigated soil. In contrast, each phosphomonoesterase displayed the same K_m in extracts, fumigated and unfumigated soils. These results show that activity of soil phosphomonoesterases is not influenced by any direct interaction with soil and, therefore, are more likely to be extracellular enzymes primarily devoted to biogeochemical cycling than are arylsulfatases.     

基于UWB定位系统在粮库中的应用分析

粮库中熏蒸投药散气、清理粮食挂壁、应急救援等仓储作业项目存在较高的危险系数,为使危险作业过程得到有效监管,提出了一种基于UWB技术的定位系统。与其它定位技术相比,该技术具有能耗低、精度高、抗干扰能力强等优点,可在粮库生产场景中得到广泛应用,进一步确保粮库安全生产,契合智能化粮库建设的需求。     

川西古镇建筑景观研究——以黄龙溪为例

通过对川西典型古镇成都市双流县黄龙溪的现场调研,从地理位置、整体布局、居住文化等方面,简要剖析了黄龙溪古镇的风水朝向、民居特色、客家建筑和干栏文化,强调了古代风水学上"负阴抱阳,背山面水"以及"四砂神"(即左青龙、右白虎、前朱雀、后玄武)的绝妙格局,展现了黄龙溪古镇朴实飘逸的建筑风格,"七街九镇"的建筑布局,飞檐翘角、鳞次栉比的民居韵律。对川西古镇建筑景观的研究和当今景观建筑设计具有较大的参考价值。     

谈林区公路中涵洞位置的设计

本对林区公路涵洞位置提出了几种设计方法。即（１）逢沟设涵;（２）改移河槽;（３）截弯曲直;（４）特殊情况下的涵洞设计。     

新型城镇化中休闲农业的定位与作用

从休闲农业的内涵与特性入手,认为它是集生产、生活、生态“三生”一体功能的新型产业,具有可持续性、科技性、文化性和产业融合性的特征。介绍了发展休闲农业与新型城镇化的关系,在实现农民市民化、就地城镇化、文化传承、生态保护和城乡统筹发展5个方面存在的意义。然后从资金保障、发展动力、劳动力资源和物质保障4个方面阐述休闲农业是如何引导新型城镇化的形成,具体介绍了休闲农业引导的新型城镇化的发展模式——旅游小城镇、旅游新农村社区和旅游卫星城镇。最后阐明休闲农业在引导过程中要注意城镇化的不同特色、合理规划和利益共享3个要点。