不同基因型香料烟叶片发育过程中碳氮代谢关键酶活性及代谢产物变化

摘 要：研究了浙江新昌不同基因型香料烟叶片发育过程中碳氮代谢关键酶的活性及代谢产物的含量变化规律。结果表明,在香料烟叶片发育过程中,酸性转化酶(INV)的活性均呈下降趋势;谷氨酰胺合成酶（GS）的活性Samsun品种在20d叶龄时最高,而Basma和Canik在30d叶龄时最高;苯丙氨酸解氨酶（PAL）的活性均表现为30d时最低;随着叶片成熟总氮含量降低,烟碱含量升高,总碳、可溶性总糖随着叶片成熟其含量呈升高趋势;品种不同,碳氮代谢关键酶的活性及代谢产物的含量变化均有很大差异。     

基于无人机遥感与卷积神经网络的草原物种分类方法

基于无人机高光谱成像遥感系统,在400～1 000 nm波段内采集低矮、混杂生长的荒漠草原退化指示物种的高光谱图像信息。分别在退化指示物种的开花期、结实期和黄枯期进行飞行实验,飞行高度30 m,高光谱图像地面分辨率2. 3 cm。采用特征波段提取与深度学习卷积神经网络相结合的方式,提出一种荒漠草原物种水平分类的方法,结合植物物候给出了中国内蒙古中部荒漠草原物种分类的推荐时相,总体分类精度和Kappa系数平均值分别达到94%和0. 91。研究结果表明,无人机高光谱成像遥感技术及深度卷积神经网络可以较好地实现荒漠草原退化指示物种的分类,与基于径向基核函数的支持向量机、基于主成分分析的深度卷积神经网络分类法相比,基于特征波段选择的深度卷积神经网络分类法效果最好,分类精度最高。无人机搭载高光谱成像仪低空遥感和卷积神经网络法提供了一种草原物种水平分类的途径。     

基于无人机高光谱遥感影像的防护林树种分类

树种信息对林业资源监测和管理具有重要意义,及时准确地掌握树种及长势状况是防护林工程建设与效益评价的基础。为研究利用无人机高光谱数据进行防护林树种分类的效果,选取典型区域使用Matrice600型六旋翼无人机搭载Rikola高光谱成像仪获取高光谱影像,基于支持向量机-递归特征消除算法（SVM-RFE）选取原始波段最佳组合,再结合纹理特征、植被指数和数理统计特征,使用随机森林算法对所有特征进行重要性评估并与分类精度相结合进行特征优化,进而构建高光谱影像全波段、原始波段最佳组合、全部特征变量、基于随机森林（RF）特征优化后特征变量4种分类方案,分别采用最大似然法（MLC）、支持向量机（SVM）、随机森林对防护林优势树种进行分类。结果表明：所提出的基于交叉验证的SVM-RFE算法选出的原始波段组合能更好地还原原始光谱特征;通过RF算法的特征重要性分析与分类精度相结合的方法可以有效选出重要特征,当使用全部特征的85%（包括17个光谱特征、3个纹理特征、5个植被指数和3个数理统计特征）进行分类时,总体精度最高为9593%（Kappa系数为0.9475）;所有特征中植被指数特征最重要,3种分类方法中RF算法分类总体精度（OA）最高。     

Polyamines enhance chilling tolerance of cucumber (Cucumis sativus L.) through modulating antioxidative system

Cucumber chilling-resistant cultivar Changchun mici and -sensitive cultivar Beijing jietou were used in this study to investigate the effects of exogenous PAs on protection against chilling injury as well as on changes of physiological features, and the fluctuation of free PAs content in the leaves under chilling stress. Upon chilling treatment, free spermidine (Spd), spermine (Spm) and putrescine (Put) were remarkably induced in the leaves of cv. Changchun mici 1 day after treatment. The induction of Put declined thereafter, whereas Spd and Spm levels increased steadily. In the leaves of cv. Beijing jietou, Put content was increased only at 1 day after chilling while Spd content decreased significantly upon chilling treatment. Chilling reduced soluble protein content, and decreased the activities of antioxidant enzymes, including superoxidase dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) only in Beijing jietou. However, these changes could be renovated by exogenous application of Put and Spd. It was also found that pretreatment with Put and Spd diminished the increased electrolyte leakage and malondialdehyde (MDA) content caused by chilling in the leaves of both cultivars. Pretreatment of methyglyoxal-bis-(guanylhydrazone) (MGBG), the PAs biosynthetic inhibitor cancelled the effects of PAs in most of the treatments. Moreover, histochemical staining and quantitative measurements showed that exogenous application of Put and Spd eliminated but MGBG exaggerated the hydrogen peroxide (H₂O₂) accumulation caused by chilling stress, especially in leaves of Beijing jietou. Interestingly, Changchun mici was found to contain higher endogenous free PAs contents compared to Beijing jietou. While no significant difference of SOD, POD and CAT activities was found between non-chilling Changchun mici and Beijing jietou seedlings, the former exhibited higher APX activity than the latter. These results suggest that PAs play important roles in the tolerance of cucumber against chilling stress, which is most likely achieved by acting as oxidative machinery against chilling injury.     

芭蕉属野生种的地理分布

芭蕉属起源于热带亚热带区域,对芭蕉属野生种质资源的研究始于20世纪40年代。通过检索INIBAP的多个数据库和相关文献,归纳整理出芭蕉属野生种的分布规律,为野生蕉的种质资源保存、利用以及品种鉴定提供一定的理论依据。经分析,目前已经报道的芭蕉属共有52个野生种,其中真芭蕉组(Eumusa)11个,观赏蕉组(Rhodochlamys)9个,澳蕉组(Australimusa)13个,红花蕉组(Callimusa)18个,不确定组(Incertae sedis)1个。马来西亚、印度尼西亚、巴布亚新几内亚、印度和中国均分布有不同的3组芭蕉属野生种,但马来西亚和印度分布的种数最多,各分布有15种,约占总数的29%,可能是芭蕉属野生种的多样性中心。     

常见易混淆园林植物的识别

在园林绿化工程中很可能因不能准确识别植物种类而造成工程质量不符合合同要求,从而带来巨大损失。工程中出现品种混乱的原因很多,文章着重论述华中地区一些常见易混淆园林植物的分辨要点。     

Comparison of sympatric freshwater turtle populations from an urbanized Sydney catchment

• 1. Australian freshwater turtles are widely distributed throughout the continent, and in each river catchment there are at least two taxa. In south‐eastern Australia Chelodina longicollis and forms of Emydura macquarii co‐habit within a waterway, although they have been shown to partition habitat within the water column in non‐urban bodies of water. Limited comparative data are available for the urban populations.
• 2. Within urban Sydney C. longicollis (eastern long‐necked turtle) and Emydura macquarii dharuk (Sydney short‐necked turtle) share habitat. However, in contrast with non‐urban studies of C. longicollis and other sympatric E. macquarii taxa, it was observed that the population profile of the two species was similar at all sites, and that C. longicollis were present in greater numbers than E. m. dharuk.
• 3. The continued degradation of preferred habitat, low recruitment, and potential competition from introduced turtles place both species in a precarious position.
• 4. The shallow, impounded waterways of the regulated urban bodies of water align more closely with the preferred habitat of C. longicollis than with that of forms of E. macquarii, which prefer deeper flowing waters or large wetlands adjacent to rivers. Emydura m. dharuk may be at greatest risk of extinction in urban areas.
• 5. Across urban Sydney, the low numbers of E. m. dharuk compared with C. longicollis may be due to the lack of mobility of E. m. dharuk such that individuals tend to be stranded in sub‐optimal habitat. In contrast, C. longicollis has a greater propensity for overland movement, and a preference for the ‘new habitat’ resulting from urban impacts on the associated waterways, and thus appears to be able to utilize these modified urban waters more successfully.

Copyright © 2008 John Wiley & Sons, Ltd.     

Conserving indigenous crayfish: stock assessment and habitat requirements in the threatened Austropotamobius italicus

• 1. As part of the Austropotamobius pallipes species complex, the crayfish Austropotamobius italicus is a species of community interest whose preservation requires the designation of Special Areas of Conservation (SACs) (Annex II, EU Habitats Directive). This study aimed at (1) assessing the conservation status of this threatened indigenous species by stock assessment in central Italy and (2) identifying some aspects of its elective habitat.
• 2. Surveys were conducted in nine streams harbouring A. italicus (streams WI) and in 10 streams where crayfish populations became extinct at least 5 years before the study (streams WO).
• 3. The results confirmed that A. italicus is a K‐selected species, with a relatively slow growth rate (males: 0.34; females: 0.37) and a long life expectancy (males: 8.2 years; females: 7.8 years). The extant populations are healthy, showing balanced sex‐ratios and well structured age‐class compositions. Mortality is mainly due to fishing, which is illegal in Tuscany.
• 4. Principal Components Analyses showed that the streams WI and WO differ in the abundance of allochthonous plant detritus but not in the taxonomic composition of their macroinvertebrate communities. Age classes were found to be spatially segregated, juveniles mainly using cobbles as substrates and adults seemingly avoiding them.
• 5. The loss of the pristine riverine landscape seems to have been responsible, together with illegal fishing, for the local extinction of the species. As a consequence, retaining, enhancing, and restoring the habitat and its complexity are required for the preservation of A. italicus.
• 6. The designation of SACs might help in this endeavour if accompanied by programmes aimed at publicizing the need for conservation of this species. Unfortunately, crayfish‐focused projects supported by LIFE in Italy since 1992 (4%) and the SACs involved (1.4%) have been relatively few, despite the poor conservation status of this species and its well recognized ecological role.

Copyright © 2008 John Wiley & Sons, Ltd.     

Crunoecia irrorata (Curtis) (Trichoptera: Lepidostomatidae) and conservation of boreal springs: indications of clustering of red‐listed species

• 1. Crunoecia irrorata is widely dispersed across Europe, but rare and red‐listed in Finland. Its geographical range comprises most of the southern boreal zone in Fennoscandia. The species is confined to springs in the north, but occurs also in lower reaches of headwater streams in central and western Europe. It becomes more rare and less abundant towards the limits of its range across Europe, showing diffusive rarity. Glacial history and climate limit the distribution of the species in the north.
• 2. The most important habitat characteristic for C. irrorata in Finland was the total area of helocrene habitats. Its occurrence was positively related to taxon richness of benthic macroinvertebrates, but not bryophytes. Co‐occurrence of C. irrorata with other red‐listed spring‐dependent taxa was observed, indicating high conservation value of the springs occupied.
• 3. Crunoecia irrorata occurred frequently in moderately disturbed springs, indicating tolerance to human disturbance around springs. However, the effect of the disturbance on population size is not known. Forestry and water and gravel extraction are potential threats to C. irrorata, and only a few of its populations are strictly protected. Therefore, populations of C. irrorata in the boreal zone should be conserved and monitored to ensure the species' survival at the northern limits of its range.

Copyright © 2007 John Wiley & Sons, Ltd.     

大亚湾大型底栖动物物种多样性现状

利用2004年3月、5月、9月和12月的大型底栖动物定量采样数据,对大亚湾大型底栖动物的物种多样性进行研究。结果表明,因人类活动影响的加剧,目前大型底栖动物多样性明显低于历史水平。2004年大亚湾海域内共出现79种（类）大型底栖动物,全海域均匀度（J）、丰富度（D）和Shannon—Wiener指数（H’）均值分别为1．17、0．74和2．06。各多样性指数的季节变化较小,不存在显著差异（P〉0．05）。多样性水平以冬季〈春季〈夏季〈秋季。大亚湾明显的干、湿季变化对大型底栖动物多样性有所影响,湿季多样性水平高于干季。湾口海域大型底栖动物多样性水平较高,因湾顶和湾中部海域内小鳞帘蛤Veremolpamicra和粗帝汶蛤Timocleascabra极强的优势地位,导致湾顶和湾中部海域多样性水平较低。水温升高而引发的小鳞帘蛤和粗帝汶蛤丰度的变化,造成了湾顶和湾中部海域大型底栖动物多样性水平的季节变化。