全文获取类型
收费全文 | 12523篇 |
免费 | 523篇 |
国内免费 | 1529篇 |
专业分类
林业 | 730篇 |
农学 | 1283篇 |
基础科学 | 600篇 |
1924篇 | |
综合类 | 5511篇 |
农作物 | 871篇 |
水产渔业 | 554篇 |
畜牧兽医 | 1681篇 |
园艺 | 790篇 |
植物保护 | 631篇 |
出版年
2024年 | 51篇 |
2023年 | 138篇 |
2022年 | 311篇 |
2021年 | 353篇 |
2020年 | 416篇 |
2019年 | 548篇 |
2018年 | 463篇 |
2017年 | 567篇 |
2016年 | 720篇 |
2015年 | 544篇 |
2014年 | 592篇 |
2013年 | 738篇 |
2012年 | 861篇 |
2011年 | 784篇 |
2010年 | 729篇 |
2009年 | 655篇 |
2008年 | 603篇 |
2007年 | 624篇 |
2006年 | 518篇 |
2005年 | 403篇 |
2004年 | 342篇 |
2003年 | 299篇 |
2002年 | 248篇 |
2001年 | 216篇 |
2000年 | 236篇 |
1999年 | 206篇 |
1998年 | 175篇 |
1997年 | 264篇 |
1996年 | 245篇 |
1995年 | 218篇 |
1994年 | 239篇 |
1993年 | 235篇 |
1992年 | 289篇 |
1991年 | 272篇 |
1990年 | 233篇 |
1989年 | 91篇 |
1988年 | 45篇 |
1987年 | 25篇 |
1986年 | 17篇 |
1985年 | 10篇 |
1984年 | 8篇 |
1982年 | 5篇 |
1981年 | 6篇 |
1980年 | 5篇 |
1979年 | 5篇 |
1978年 | 5篇 |
1977年 | 2篇 |
1962年 | 3篇 |
1956年 | 5篇 |
1955年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
61.
J. Guan J. C. Kapteyn A. Kerkenaar M. A. De Waard 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(5):313-324
Differential accumulation of [14C]imazalil and [14C]fenarimol by germlings of wild-type and DMI-resistant isolates ofPenicillium italicum was studied at various pH values. At pH 7 and 8 the low-resistant isolate E300–3 accumulated 22% and 35%, respectively, less imazalil than the wild-type isolate W5. Imazalil accumulation at pH 5 and 6 was similar. Isolate E300–3 also accumulated less fenarimol as compared with the wild-type isolate. This difference was much more obvious than for imazalil and was observed at all pH values tested. Differences in accumulation of both imazalil and fenarimol between low (E300–3), medium (H17) and high resistant (I33) isolates were not observed. These results suggest that decreased accumulation of DMIs is responsible for a low level of resistance only and that additional mechanisms of resistance might operate in isolates with a medium and high degree of resistance. With all isolates fenarimol accumulation was energy-dependent. This was not obvious for imazalil.The wild-type and DMI-resistant isolates had a similar plasma membrane potential as determined with the probe [14C]tetraphenylphosphonium bromide ([14C]TPP+). Various test compounds, among which ATPase inhibitors, ionophoric antibiotics and calmodulin antagonists, affected the accumulation of [14C]TPP+, [14C]imazalil and [14C]fenarimol. No obvious correlation between the effects of the test compounds on accumulation levels of the fungicides and [14C]TPP+ could be observed. These results indicate that the plasma membrane potential does not mediate the efflux of DMI fungicides byP. italicum. 相似文献
62.
简怀玉 《湖南农业大学学报(自然科学版)》1990,17(3)
本文研究一类2k阶非线性偏微分方程组之解的正则性,没有假定通常的椭圆性条件而只假定所谓"无穷远处"的椭圆性条件,证明了解的k-1阶导数为李普希兹连续的. 相似文献
63.
Although the molluscicide Frescon is a strong neurotoxin to the Lymnaea stagnalis central nervous system in vitro, it is probable that the exposure of the whole animal to this molluscicide fails to result in central nervous system abnormalities: Frescon does not appear to reach the brain in sufficient quantity to disrupt its normal activity. However, only those Frescon analogs found to be neurotoxic were molluscicidal, suggesting some related mode, if not site, of action. Frescon and its analogs may act by affecting excitable tissues other than the nervous system (e.g., the snail musculature) by altering certain functional and/or structural membrane properties. 相似文献
64.
65.
AIM:The effective antisense sequences targeted VEGF mRNA with computer software would be screened and designed, and effect of them on growth K562 cells and protein expression of VEGF were studied with experiments.METHODS:Seven antisense sequences were selected and synthesized, which consisted of 18-20 deoxynucleotide acid and were modified with phosphorothioate, according to principle of low free energy of overall △G37 Overall. Cell growth was assayed by trypan blue dye exclusion assay and level of VEGF protien in the media was determined by ELISA.RESULTS:Six of seven sequences were capable of inhibing growth of K562 cells and downregulating the VEGF protein expression significantly, compared with Scrambed control group. It was found that there was a close correlation between low level of overall △G37 and antisense effectiveness (r=0.887,P<0.01).CONCLUSION:VEGF mRNA antisense oliogdeoxynucleotides, which were designed by computer software of RNAstructure, were able to inhibit growth of K562 and its protein expression. The VEGF mRNA may be new target attached by drugs. At same time, the computer aided design is useful methods to obtain the effective antisense. 相似文献
66.
中国风水侵蚀交错区分布特征分析 总被引:6,自引:1,他引:6
风水侵蚀交错带的研究 ,对景观、土地覆盖 /土地利用、全球变化等研究具有重要意义。论文以GIS技术为支撑 ,定量计算出我国风水侵蚀带。在分布特征上 ,主要分布我国北方地区 ,其中以西部沙漠 ,北部山脉沿线为重 ,这种分布与气候、地貌分界有明显联系。 49.6 %风水侵蚀复合带的降水量小于 2 0 0mm。风水侵蚀复合带的土地利用主要以草地为主 ,占 41.8% ,其次为耕地。两种类型的分布 ,草地以与其它土地利用类型交错分布为主 ;而耕地是有重心的分散分布。风水侵蚀复合带的总体侵蚀要强于全国水平。 相似文献
67.
AIM: To investigate the protective effect of onychin on the endothelial cells injured by oxidative stress. METHODS: The injured model was established by endothelial cells treated with menadione. The growth inhibitory rate of endothelial cell was determined by MTT assay; NO2-/NO3- concentration in the medium was determined by nitrate reductase assay; eNOS and caveolin-1 protein levels were determined by Western blot. RESULTS: Onychin significantly decreased the growth inhibitory rate of endothelial cells injured by menadione, increased NO2-/NO3- concentration in the medium and eNOS activity and up-regulated caveolin-1 expression. CONCLUSION: Onychin possesses a protective effect against endothelial cell injury induced by menadione via caveolin-1/eNOS pathway. 相似文献
68.
AIM:To investigate whether the bcl-2 antisense oligonucleotide increases the sensitivity of HL60 and K562 cell lines to daunorubicin.METHODS:IC50 for HL60 and K562 was determined with MTT method, the expression levels of Bcl-2 protein were assayed by immunofluorescence using fluoresce isothiocyanate labeling. In addition, apoptosis was detected by morphological observation and flow cytometric analysis of DNA fragmentation.RESULTS:It was found that the two oligonucleotides directed against the coding region and the translation initiation of bcl-2 mRNA, combined respectively with daunorubicin, inhibited expression of bcl-2 protein, increased apoptosis in HL60 and K562 cells, and decreased IC50 of daunorubicin significantly (P<0.05). Compared to the antisense oligonucleotide directed against the translation initiation of bcl-2 mRNA, the antisense oligonucleotide directed against the coding region showed stronger effects in the aspects of increasing the sensitivity of HL60 cells to daunorubicin (P<0.05).CONCLUSIONS:These two antisense sequences in the translation initiation and the coding region of bcl-2 mRNA increased the sensitivity of HL60 and K562 cell lines to daunorubicin in a sequence-specific manner. 相似文献
69.
AIM:To investigate the effects of Liucha extract on the growth of tumor cells in vitro and its possible mechanism.METHODS:The capability of colony forming of human leukemia K562 cell in vitro and the cells metabolism were studied by semi-solid agar culture and MTT staining. Then , the changes in morphology in the tumor cells were examined using electronic microscope.RESULTS:Semi-solid agar culture and MTT colorimetric analysis showed that Liucha extrats could significantly inhibit the growth of the tumor cells and their capability of colony forming. Also,under the electronic microscope,it was found that the tumor K562 cell had a narrower perinuclear space,condensation of chromatin and an enlarged mitochondria , in which the cristase disappeared.CONCLUSION:The extract from Liucha possesses an inhibitory effect on K562 cell growth in vitro through affecting the metabolism of the tumor cells. 相似文献
70.