Molecular characterization of RNA and protein synthesis during a one-step growth curve of bovine viral diarrhoea virus in ovine (SFT-R) cells

The aim of this study was to determine the kinetics of noncytopathic bovine viral diarrhoea virus (BVDV) multiplication and synthesis of BVDV specific RNA and proteins in ovine cells (SFT-R) during a one-step growth curve. The virus titre and RNA level were determined by focus-forming assay and real time RT-PCR. The RNA synthesis was detected by Northern blot while synthesis of E2 and NS3 proteins was assayed by immunohistochemistry and Western blot. The results showed that synthesis of viral RNA is initiated at 4 h, NS3 and E2 proteins are detectable at 6-7 h and the replication cycle is complete at 10-12 h. Additionally, we provide evidence that NS2-3 protein was cleaved in ovine cells early during infection and in proliferated leukocytes of acutely infected sheep. This study showed that synthesis of BVDV RNA and proteins in ovine cells occurs at similar times as found in bovine cells.     

手机在林业科技活动中的应用初探

针对林业科技活动中记录采集数据受制于工作条件的问题,提出使用手机彩信、录音、照相、录像等功能记录采集数据的方法和途径,以及进行远程咨询的设想,供林业科技人员参考。     

High-credibility RFID-based animal data recording system suitable for small-holding rural dairy farmers

In order to remain globally competitive and to ensure traceability, intensive and extensive livestock operations are adopting radio-frequency-based electronic identification (RFID) and data recording systems. Such integrated systems offer dual advantages of lowered labor costs due to automation and enhanced profits due to optimization of animal productivity, health and welfare. However, RFID-based systems might not be economically viable for small-hold livestock farmers unless there is considerable value advantage. Further, the set up and operation of a data recording system for small-hold farmers is also difficult due to size-constraints and distant farm units. We have developed an integrated system for small-hold dairy farmers to enable employing of RFID technology to ensure credibility of data recording, and avoidance of livestock insurance-related claim malpractices. The system can additionally be used to periodically collect performance records and to operate veterinary service delivery. The integrated system comprises of: (a) an RFID tag or insert; (b) an RFID reader; (c) a PDA/mini-laptop with custom software installed; (d) a USB modem internet connection; and (e) a central data server on web platform with dedicated server-level software. The unique feature of the system is that the veterinary health worker (VHW) is able to register and enter new records only when the RFID reader connected to a mini-laptop is within reading range of the associated RFID tag. This also authenticates the visit by the VHW. Other data management operations such as browsing, sorting, data analysis and report generation can be carried out when the VHW is away from the RFID field. We have deployed and validated the system in a cluster of 5000 dairy animals spread over more than 10 villages with an average of two to three animals per farmer in Thanjavur district, Tamil Nadu, India. The system is user-friendly and easy to operate in that the animals’ insurance registration and issuance of policy documents can be done in a single farm visit. The system can also be used for collecting periodic animal records and sending SMS ‘alerts’ to the farmers. Initial economic analysis suggests that the investment cost would be recovered even if fraudulent claims in around 0.5% of the insured animals can be prevented. The sustenance cost can be recovered from the improvised health and production management service delivery to the farmers. It is however emphasized that the system can only be implemented in organized dairy operations wherein the milk processing company can establish functional collaboration with veterinary service providers, insurance company micro-finance companies and this consortium can bear the cost of RFID in exchange for long term multilateral benefits to all the stakeholders.     

抗菌肽SMAP-29基因密码子优化及其在毕赤酵母中的表达

[目的]对抗菌肽SMAP-29的基因密码子进行优化,并使其在毕赤巴斯德酵母(Pichia pastoris)中表达。[方法]根据已知抗菌肽SMAP-29的氨基酸序列,参照毕赤巴斯德酵母密码子的偏好性,设计合成抗菌肽SMAP-29成熟肽基因片段,并同载体pPIC3.5K连接,电击转化至毕赤酵母受体菌GS115中,经G418筛选高拷贝转化子,并用MM、MD板和PCR法筛选Mut+表型。用甲醇诱导表达构建好的重组酵母表达基因工程菌,并裂解酵母细胞进行Tricine-SDS-PAGE分析。[结果]在诱导至第2天的细胞裂解液中检测到与预测的SMAP-29分子量相当,约为3.2 kD的表达带;表达产物对金黄色葡萄球菌和白色念珠菌有明显的抑菌作用,而对大肠杆菌抑菌效果不明显。[结论]该研究为SMAP-29在生物医学、农业等领域中的应用奠定基础。     

杏鲍菇菌丝体胞内与胞外多糖体外抗氧化活性研究

采用Fenton法、邻苯三酚自氧化法、还原力的测定以及盐酸萘乙二胺比色法等几种实验方法研究了杏鲍菇菌丝体胞内与胞外多糖体外抗氧化活性。结果表明：杏鲍菇菌丝体胞内与胞外多糖提取物均具有较强的体外抗氧化性能,且随着多糖浓度的增大其抗氧化活性逐渐增强。对羟基自由基（·OH）的清除能力,胞内多糖优于胞外多糖;对超氧阴离子自由基（O2）的清除能力,胞外多糖优于胞内多糖;在还原力抑制方面,胞外多糖优于胞内多糖;在对NO2－清除方面,胞外多糖表现为最强;在卵黄脂蛋白不饱和脂肪酸过氧化体系中胞外多糖抗氧化能力强于胞内。     

高性能低功耗智能流速流量仪系统的设计

为满足水文部门对河流流速流量测量多种的要求,在旋浆式流速仪基础上,设计了以P98V51为核心的高性能低功耗智能流速流量仪系统,介绍了该仪表的原理和软硬件设计方法并设计了上位机系统.应用表明,该流速流量仪实现了提高测量精度,降低仪表功耗,提高测量数据时效性,无纸记录,差水质条件下测量的要求.符合国家河流流量测验规范要求.     

基于Android平台的土壤采样信息自动记录系统

目前土壤采样信息大多依赖于实验人员手动纸质记录。为了更好地记录和处理土壤信息,提出了一种适用于现代化农业的土壤采样信息记录方法,利用Android系统结合服务器和数据库架构实现基于Android的土壤采样信息自动记录系统。整个系统设计包括系统架构设计和软件功能设计。在服务器上使用JSP和MySQL数据库管理系统（Database management system,DBMS）作服务器端,搭建云服务器及农田信息管理数据库。服务器端设计包括在WEB工程中引入JDBC驱动,使服务器直接连接数据库,实现WEB服务器与MySQL数据库的交互。Android客户端设计包括在Material Design设计规范下完成土壤自动采样记录系统Android客户端界面,以及利用JSON数据解析实现对云服务器下MySQL数据库信息的访问和操作。最后对系统软件进行有效性和健壮性测试。试验表明,该系统能有效地显示采样土壤位置的空气温度、湿度、经纬度以及土壤氮素含量等土壤采样信息,并能进行正确的地图位置显示,证明了Android系统在土壤采样记录方面的有效性以及构建系统的可行性。     

Amphibian conservation in Scotland: A review of threats and opportunities

相似文献   

胡杨悬浮细胞原生质体游离及质膜单离子通道测定

该文以胡杨 (Populuseuphratica)悬浮细胞为材料 ,利用 1 0 %纤维素酶“onozuka”R 10、0 0 1%果胶酶Y 2 3、0 15 %离析酶R 10和 0 1%半纤维素酶的混合酶液消化细胞壁 ,得到原生质体 .并利用膜片钳细胞贴附技术分别测到质膜内向和外向单通道电流 .通道电流在不同水平上变化 ,表明并非只有一种类型的通道开放 .树木细胞原生质体的分离以及利用膜片钳测定细胞质膜离子通道的成功实践为深入研究木本植物抗盐的细胞学机制奠定了基础     

抗菌肽BSN-37对大肠杆菌胞内物质泄露的影响

为了解抗菌肽BSN-37对大肠杆菌的作用机制,通过β-半乳糖苷酶试验测定了抗菌肽BSN-37对大肠杆菌CVCC1568的抑菌活性,以菌落计数法测定了抗菌肽BSN-37对大肠杆菌CVCC1568的抑菌动力学,用分光光度法测定了抗菌肽BSN-37对大肠杆菌CVCC1568细胞壁通透性,以及胞内紫外吸收物质、蛋白及核酸的泄露量。结果表明,抗菌肽BSN-37能有效抑制大肠杆菌CVCC1568的生长繁殖,随抗菌肽浓度增加,抑制活性越高,抗菌肽BSN-37作用后的大肠杆菌CVCC1568细胞壁通透性增加,胞内紫外吸收物质、蛋白和核酸的泄露量随时间延长而逐渐增加。说明抗菌肽BSN-37能破坏大肠杆菌CVCC1568的细胞壁和细胞膜,从而表现较好的抑菌活性。