Plant biotechnology: A case study ofBacillus thuringiensis (Bt) and its application to the future of genetic engineered trees

Agricultural productivity may be raised in a sustainable way by many different technologies such as biological fertilizers, soil and water conservation, biodiversity conservation, improved pest control, and changes in land ownership and distribution. Of these measures, biotechnology applications probably hold the most promise in augmenting conventional agricultural productivity, because biotechnology applications give not only the need to increase production, but also protect the environment and conserving natural resources for future generations. Biotechnology applications will have the possibilities to increase productivity and food availability through better agronomic performance of new varieties, including resistance to pests; rapid multiplication of disease-free plants; ability to obtain natural plant products using tissue culture; diagnosis of diseases of plants and livestock; manipulation of reproduction methods increasing the efficiency of breeding; and the provision of incentives for greater participation by the private sector through investments. Insect resistance through the transfer of a gene for resistance fromBacillus thuringiensis (Bt) is one of the most advanced biotechnology applications already being commercialized in many parts of the world. This paper reviews the development and the status ofBt technology and application ofBt transgenic plants in current agriculture, and discusses specific issues related to the transfer of the technology to the future of genetic engineered trees with emphasis on conifers. Biography: Tang Wei (1964-), male, Ph. Doctor, Research associate, Department of Biology, Howell Science Complex, East Carelina University, Greenville, NC 27858-4353, USA. Responsible editor: Chal Ruihai     

锈色粒肩天牛与寄主树种关系的初步研究

在连续 3a的定位观测和分析基础上 ,本文初步报道了锈色粒肩天牛与文献报道中的几种寄主树种之间的关系。结果如下 :(1)根据室内饲养下成虫对不同树种的取食及产卵情况和在不同树种上接种 (卵 )的幼虫情况 ,可将几种寄主树种分为 4类 :高感树种 (国槐 )、低感树种 (云实、黄檀 )、抗虫树种 (刺槐 )、免疫树种 (旱柳 ) ;(2 )酚酸类物质在旱柳中的含量 (3 0 3～ 9 73mg·(10 0g) - 1 ) ,显著高于其它树种中的含量 (1 71～5 39mg·(10 0g) - 1 ) ,可能是锈色粒肩天牛在旱柳上表现拒食、拒产卵、拒钻蛀的重要原因。氨基酸含量较高、组分相对最全 (17种 ) ,以及较高的C N值 ,是国槐基本营养物质的显著特征 ,这可能是锈色粒肩天牛嗜食国槐 ,对其造成严重危害的根本原因。锈色粒肩天牛很少取食危害刺槐 ,这与刺槐树种的各部分C N值过低 ,造成营养失调有关。黄檀木材纤维平均长度为 0 837mm ,平均宽度为 18 90 μm ,长宽比为 5 0 96 ,其韧性是参试树种中最大的 ,因而可能造成幼虫啃食困难 ,加上木材中的C N低 (0 36 ) ,因此 ,具有较强的抗虫性。云实树皮薄而光滑 ,造成成虫产卵困难 ,因此 ,在自然状态下 ,锈色粒肩天牛不易对其产生危害。     

云南木蠹象的危险性分析

根据云南木蠹象Pissodes yunnanensis的生物学特性，采用有害生物危险性分析(Pest Risk Analysis，简写PRA)方法，从该虫的分布状况、潜在危险性和寄主的经济重要性等方面，对其进行了危险性分析与评估，结果表明：云南木蠹象在我国西南地区属于中度危险的森林有害生物。     

树木嫩枝角,栓质层量与抗两种星天牛的关系

应用组织化学方法测定了６３种树种嫩枝中的淀粉，蛋白质，角质，栓质，单宁含量，经与天牛成贝选择取食量进行对比分析，结果说明，枝条中角质层，栓质层厚者抗虫，含量低者感虫，可作为选择抗虫性树种的指标之一。而蛋白质和淀粉相对含量与在牛选择取食量无关。     

昆虫病原线虫对木麻黄毒蛾侵染能力的初步研究

本文报道应用昆虫病原线虫Steinernema spp.室内对木麻黄毒蛾的侵染能力。通过斯氏属九个线虫品系对木毒蛾的侵染力测定筛选出S.feltiae Agriotos为最佳品系;能寄生低、中、高龄幼虫及蛹;可在3天内把害虫致死。供试的其它八个线虫品系也能不同程度地致死害虫,并在死虫体内繁殖。S.f.Agriotos线虫的剂量对木毒蛾的致死速度及效果有影响,以1000条线虫/害虫侵染期线虫为最适宜剂量,林间对木毒蛾幼虫的致死率平均为89.6%。     

植物生物技术:苏云金杆菌抗虫基因的研究和它对未来树木遗传工程的影响

许多不同的技术可以促进农业生产力的持续增长，生物技术是其中最重要和最有前途的方法。因为生物技术不仅可以增加生产力。而且在环境和自然资源的保护中起重要作用，应用生物技术可以生产高产、优质、高抗性和无病植物，同时也可以提高育种效率和促进食品的工业化生产。苏云金杆菌抗虫基因是目前生物技术领域中应用最广泛的例子之一。由此而产生的抗虫植物正在广泛应用于世界各地的商业化生产。本文综述了苏云金杆菌抗虫基因的研究进展和它在未来林木遗传工程中的应用。表4参68。     

Development, manufacture and application of Beta-Cypermethrin aqueous capsule suspension

The optimal formula of Beta-Cypermethrin aqueous capsule suspension was screened out through the experiment,preparation and manufacture of basic formulas, which has no sedimentation, suspended drops of oil, peels and agglomerating and the diameter of capsule is in range of 10-30μm, conformed to the technical requirement. The aftereffect of the 3.3% Beta-Cypermethrin aqueous capsule suspension which was manufactured according to the optimal formula was up more than 25 days. The experiments on controlling the larvae of Dendrolinmus superans Butler and the adults of Xylotrechus rusticus L were carried out with different concentrations of this chemical. The death rate reached 80% when 250 times solution of the chemical was sprayed on stem to control the larvae of D. superans. For control of the adults of X. rusticus, 200, 400 and 600 times solution of the chemical were applied and their control effects (death rate) reached 85.23%, 74.21% and 66.59% respectively. Two kinds of solution (200 times and 300 times) of the chemical were used to control the larvae of D. superans in large area, and the control effect for both concentrations was over 90%.     

银中杨抗病虫性研究

用室内外接种及自然感病的方法，对银中杨抗烂皮病进行测定，采用系统聚类方法进行分析，结果证明银中杨为感病最轻品种，聚在第一类，并对鉴定林中　１６个品系的病情指数作方差分析，结果表明各品系间感病指数有极度显著差异。同时对银中杨及对照品种的虫害按五级分类标准进行调查并计算受害指数，结果表明银中杨是抗杨干象、白杨透翅蛾等虫害的优良树种。     

人工林害虫的生态控制策略

森林害虫的科学管理必须考虑长远的经济效益和社会效益问题，只有对森林害虫进行生态控制才能达到森林可持续经营的目的。在人工林构建中，只有模拟天然林结构，从害虫栖息地抗虫林分结构出发，才能从根本上解决猖獗成灾问题。在对黑龙江省主要造林树种的天然抗虫结构、人工混交林和人工纯林的结构及害虫发生情况进行对比分析的基础上，提出了人工林害虫生态控制策略。     

影响松毛虫赤眼蜂种群消长因子研究

１９８６－１９９０年的研究得知：松毛虫赤眼蜂在浙江于３月下旬开始活动，１０月上旬至１１月陆续以蛹在马尾松毛虫、云南松毛虫等虫卵中越冬在林中常年都可见到该蜂的活动，其种群数量出现的高峰期与松毛虫卵期相一致，林中昆虫卵的多少、蜜源植物的有无、温度的高低、是影响松毛虫赤眼蜂林间种群消长的主要因子。