Factors affecting seroprevalence of Toxoplasma gondii in the endangered Iberian lynx (Lynx pardinus)

Wild felids are considered important in maintaining the sylvatic cycle of Toxoplasma gondii. Although, T. gondii antibodies have been reported in several species of wild felids, little is known of the epidemiology and risk factors associated with T. gondii infection in wild cats. The Iberian lynx (Lynx pardinus) is the most endangered felid species in the world. In the present study, seroprevalence and associated risk factors for T. gondii infection in a large population of Iberian lynx in Spain were determined. Serum samples from 129 Iberian lynx collected from 2005 to 2009 and 85 wild rabbits (Oryctolagus cuniculus), sharing the habitat with the Iberian lynx, were tested for antibodies to T. gondii by the modified agglutination test (MAT) using a cut-off value of 1:25. Antibodies to T. gondii were found in 81 of 129 (62.8%) Iberian lynx. Seroprevalence to T. gondii in Iberian lynx significantly increased with age (P < 0.001). T. gondii seroprevalences were similar in free-ranging (66.7% of 93) and wild-caught captive lynx (69% of 84) but significantly lower in captive-born lynx (22.5% of 40). Seroprevalence was higher in lynx with concurrent Cytauxzoonfelis (88% of 25) but not with concurrent Feline Leukemia Virus (FeLV) infection (53.8% of 13). There were no significant differences in seroprevalence between sexes, geographic region and year of sample collection (2005–2009). Oocysts of T. gondii were not detected microscopically in fecal samples from 58 lynx. Wild rabbits are considered the most important food for the lynx. Antibodies to T. gondii were found in 14 (11.9%) of 85 rabbits tested. The present results indicate that T. gondii infection is widespread in the two areas where Iberian lynx survive in Spain. The fact that four captive-born lynx seroconverted was indication of contact with T. gondii also in the Captive Breeding Centers, hence, control measures to prevent T. gondii infection would be necessary in these centers.     

Risk screening of non‐native freshwater fishes in Croatia and Slovenia using the Fish Invasiveness Screening Kit

The Fish Invasiveness Screening Kit (FISK) version 2 was used to assess the invasiveness potential of 40 introduced and translocated freshwater fish species to Croatia and Slovenia. Based on a priori classification of invasiveness, receiver operating characteristic analysis of FISK scores from two independent assessors resulted in a statistically significant calibration threshold of 11.75. This indicated that FISK was able to discriminate reliably between non‐native species likely to pose a high risk of being invasive and those likely to pose a medium or low risk of invasiveness. Seven species were categorised as ‘medium risk’ and the other 33 as ‘high risk’, whereas no species was categorised as ‘low risk’. The two highest scoring species were European catfish Silurus glanis and North African catfish Clarias gariepinus. Mean scores for all species classified a priori as invasive were ranked as ‘high risk’ sensu lato and fell into the ‘moderately high risk’ subcategory. FISK proved to be a valid tool for assessing the risks posed by non‐native fishes in Croatia and Slovenia. For this reason, it can be adopted as a reliable tool for the prevention of new translocations or introductions of potentially invasive species in the risk assessment area, as well as to assist in decisions regarding future management (i.e. monitoring, control and eradication) and conservation strategies.     

Predicting early life connectivity of Antarctic silverfish,an important forage species along the Antarctic Peninsula

The early life stages of the Antarctic silverfish (Pleuragramma antarctica), an important prey species for higher predators in the Southern Ocean ecosystem, dominate the larval fish assemblages of the Bransfield Strait, one of the most important areas for larval retention off the Antarctic Peninsula. Nevertheless, the spatial location of areas where they were spawned and the timing of larval hatching remain unknown. By linking Lagrangian particle tracking simulations with age data obtained using otolith microincrements from fish caught north of Joinville Island in a pelagic survey, we estimated the distribution of hatch dates and subsequent growth rates of silverfish reaching the Bransfield Strait, and predicted the areas where they were spawned. Larval hatching peaked during the last week of December, and the inner shelf and shelf break, east of the Larsen Ice shelf, were the dominant areas predicted to contribute to larval assemblages in the Bransfield Strait. Over simulated periods of 600–630 days, 35–40% of particles remained within the Bransfield Strait, suggesting an important source of supply to higher predators feeding off the northern Antarctic Peninsula. The daily growth rate at the mean size of 22.3 mm was 0.18 mm, corresponding to a daily change in size of approximately 0.82% standard length (SL), and large variability in growth rate suggested a wide range of environmental conditions experienced during the period of advection from the spawning areas. These results provide spatial predictions that can be tested empirically in future studies, using the simulated trajectories to inform sampling design and spatial coverage.     

基于流域的山区景观格局分析和分区研究--以山东省栖霞市为例

本文以位于胶东半岛中部山区的栖霞市为例,在对整个研究区进行流域提取的基础上,分别统计各流域内的景观指数,并通过聚类分析对整个栖霞市的山区景观进行分区。首先解译2010年Thematic Mapper(TM)遥感影像数据,获得栖霞市景观格局图,以1︰25 000地形图为基础数据源,建立DEM模型,利用ArcGIS水文分析模块将研究区划分为300个子流域。然后根据常用景观格局指数所反映的生态学意义和景观特征信息,并结合研究区内的具体景观结构,选择边缘密度(ED)、多样性指数(SDI)、斑块大小变异系数(PSCOV)、面积加权平均分维数(AWMPFD)和核心区密度(CAD)5个景观指数,并结合各流域的平均高程与空间分布对每一个子流域进行景观格局分析,探讨各子流域景观格局的空间差异,揭示人类活动、自然干扰等各种景观生态机制对区域景观生态的影响。研究结果为:ED值和CAD值随着海拔的增加而逐渐变大,垂直分异特征明显;SDI值呈现出由低海拔向高海拔逐步减少的趋势;PSCOV值随海拔的增加呈现出类似倒"U"形曲线;AWMPFD值不具有明显的垂直分异特征。最后将这5个指数进行聚类分析,同时利用流域主体景观类型对栖霞市进行景观格局分区,各土地利用类型被划分为3个景观生态类型区。研究结果为:一类区面积最小(10.14%),该区域是农作物种植的稳定农业区,景观结构和规模都很稳定,需要继续保护;二类区面积最大(50.93%),各种景观类型呈现空间交错分布,斑块形状复杂,属于自然景观和人文景观的过渡区域,需要加强该区域的土地规划和产业合理布局;三类区面积较大(35.92%),主要集中在中高海拔区域,林地和草坡地居多,开垦难度较大。分区结果能够较好地反映研究区景观生态结构的显著特征与空间分异状况,对栖霞市的土地管理和规划具有一定的指导意义。     

雷州半岛近岸海水水质评价

基于2012年对雷州半岛近岸水域海洋环境调查的结果,对其近岸海水水质进行评价。各因子一类海水平均达标率为75．12％。Cd、Cr^6＋、Zn．As和挥发性酚的一类海水达标率为100．00％;Hg的一类海水达标率最低,为11．67％;P、Pb、悬浮物的达标率也偏低,分别为45．00％、56．67％和58．33％。雷州半岛西南部近岸海域水质最好,西部海域水质好于东部。东部水域中,雷州湾水质好于湛江港和新寮湾。雷州半岛轻污染水域较多,湛江港、新寮湾附近海域水质最差。     

Differential weathering of granitic stocks and landscape effects in a Mediterranean climate,Southern Iberian Massif (Spain)

Mineralogical and structural features control the weathering processes and landform development of two different crystalline stocks in the Iberian Massif, Spain (the Santa Elena and Linares stocks). The Santa Elena stock shows irregular saprolite profiles and boulder landforms whereas the Linares stock develops broad and plain landscapes with uniform saprolites and less boulder forms. The Santa Elena stock is more closely jointed and fractured than the Linares body. The main secondary minerals are kaolinite, and illite; illite/smectite interstratification was only observed in deep samples from the Linares profile. The Linares profile is feldspar-enriched whereas in Santa Elena plagioclase is almost absent and clay mineral content, especially kaolinite, is higher. Feldspars show dissolution channels developed along cleavage planes filled by clay minerals. Biotite–kaolinite intergrowths have fanned-out textures of epitaxial disposition. Weathered materials are enriched in Al and H₂O and Fe, and depleted in Ca, Na, Mg, Ti, P. The Santa Elena weathered materials are richer in H₂O than those from Linares and have lower Si/Al ratios. Mineralogical and geochemical evidence indicate the Santa Elena materials are more intensely weathered. High fracturation and high Ca-richer plagioclase contents are key factors producing the pervasive Santa Elena stock weathering. Fluvial erosion removed the alteration products in incised tectonically controlled streams resulting in boulder accumulation.     

The regeneration status of the endangered Acer opalus subsp. granatense throughout its geographical distribution in the Iberian Peninsula

Acer opalus subsp. granatense is an endemic endangered tree with a wide but fragmented distribution in the Mediterranean mountains. The persistence of its small populations often depends on just a few adults, and consequently is highly vulnerable to factors limiting recruitment. In this paper, we examined the regeneration status of this maple in 16 populations throughout its whole geographical distribution in the Iberian Peninsula. Age and size structures were analysed as indicators of the viability of the species. Additionally, we studied the effects of herbivory by ungulates and the role of shrubs as nurse plants in maple regeneration dynamic. Our results show that A. opalus subsp. granatense has active recruitment throughout its range in the Iberian Peninsula. Shrubs served as the main microsites for recruitment, not only for early establishment but also for long-term survival. However, ungulates heavily damaged maple saplings in all locations and microhabitats. A direct consequence of herbivory is the uncoupling of age and size structures, saplings becoming older but not higher, possibly affecting population turnover in the long term. We suggest that the conservation of the small populations of Acer opalus subsp. granatense heavily depends on the control of herbivory pressure throughout the maple distribution area.     

Age and growth of threeline grunt <Emphasis Type="Italic">Parapristipoma trilineatum</Emphasis> along the south-western coast of Kii Peninsula,Japan

Age and growth were determined for threeline grunt Parapristipoma trilineatum based on otolith readings of 1043 specimens collected along the south-western coast of Kii Peninsula, Japan. Observations of the otolith margin, together with comparisons of ring mark counts with age in artificially reared specimens, verified that such marks were produced once a year. The formation period corresponded approximately to the main spawning season (May–June). Both surface and cross-section methods were used for age determination; the surface method often resulted in underestimations of age for fish older than 2 years. Discrepancies between the two methods became greater with fish growth. The von Bertalanffy growth curve, based on ages inferred from the cross-section method, was FL _t=331×{1−exp[−0.283×(t+1.45)]}. No substantial difference was detected between male and female growth parameters. The oldest maximum ages inferred from the cross-section method were 15 and 21 years for females and males, respectively. It seems likely that P. trilineatum grows more slowly and lives longer than previously thought, and the difference is attributed primarily to differences in aging methods used in the present and previous studies.     

Microhabitat use by Iberian nase Chondrostoma polylepis and Iberian chub Squalius carolitertii in three small streams, north-west Portugal

Abstract –  Microhabitat use of Iberian nase Chondrostoma polylepis (Steindachner 1865) and Iberian chub Squalius carolitertii (Doadrio 1988) was studied in three small streams of the Lima basin, north-west Portugal. Measurements were made for 210 and 248 individuals, respectively, distributed in two size classes, based on fish ages, during winter, spring, summer and autumn 1998. Both species moved to shallow and slow-flowing microhabitats in summer and autumn, but this mainly reflected seasonal variation in microhabitat availability. Larger individuals of both species used deeper and lower percentage cover areas than smaller conspecifics, however significant ontogenetic differences were only found for water velocity. Different patterns of nonrandom microhabitat use were displayed by both cyprinids. Larger I. nase were in deeper and more discovered waters than those available in the habitat, whereas I. chub was over-represented in slow-flowing areas. There was no evidence of interspecific competition for microhabitat, therefore it is unlikely that spatial resource was limited during the present study.     

Assessing the role of the aquatic Natura 2000 network to protect both freshwater European species of community interest and threatened species in a Mediterranean region

1. The European Natura 2000 (N2K) network of protected areas stands out as the main conservation strategy in the European Union to preserve biodiversity under the auspices of the Convention on Biological Diversity.
2. The management of N2K sites is mainly focused on protecting the biological elements as Special Areas of Conservation for habitats or species listed in the European Habitats Directive, or as Special Protection Areas for birds listed in the European Birds Directive for which the site was designated. It seems urgent, therefore, to discriminate which N2K sites are really providing effective protection for aquatic biodiversity.
3. Although the main objective of N2K is to protect species listed in the Birds and Habitats Directives, the conservation status of threatened species included in the European Red List of the IUCN must also be a concern for the European Union.
4. Focusing on Central Spain as a case study, the aim of this work was to evaluate how far aquatic N2K (i.e. N2K sites designated for the occurrence of freshwater elements) is effective in protecting both those aquatic species in the Directives [referred to here as Species of Community Importance (SCI) and threatened species (TS)].
5. Most of the N2K sites in the study area have been designated using aquatic elements, which means that N2K could theoretically provide appropriate mechanisms to preserve aquatic biodiversity. However, N2K provides a low percentage of overlap with some of the hotspots identified for both TS and SCI. Surprisingly, TS are in general similarly or even better represented than SCI by N2K.
6. N2K may provide good coverage for aquatic biodiversity, mainly by acting as an umbrella for other species, but it could be improved by addressing some spatial gaps, thereby making it more relevant to current challenges such as climate change and other human impacts.