重金属污染对土壤微生物种群数量及活性的影响

通过综述重金属污染对土壤微生物种群数量及其生物活性方面的影响,指出土壤重金属污染会破坏微生物种群,降低微生物活性,抑制微生物生长,进而影响土壤质量。在此基础上,讨论了微生物活动又是评价土壤环境质量的敏感性指标。     

重金属致植物矿质营养元素代谢异常的生理效应

重金属胁迫会导致植物体细胞的矿质营养元素缺乏、代谢过程紊乱,甚至可引起植物的死亡。综述了重金属导致植物体的某些矿质元素代谢异常的生理效应。     

贵州尾矿区野生豆科牧草根瘤菌及其抗性测定

为了筛选获得对复合重金属具有耐受能力且能在本地豆科植物根系内定殖共生的抗性根瘤菌,本研究从贵州省内的锰矿区、铅锌矿区采集野生豆科牧草根瘤样品,经分离纯化、菌体形态观察、16S rRNA基因序列比对分析,获得71个根瘤菌保存菌株。系统发育和区系分析结果表明,分离菌株分属7属28种,其中根瘤菌属(Rhizobium)的分布频率高达54.93%,为优势属,木兰根瘤菌(R.indigoferae)的分布频率最高(15.49%),为优势种。选择来自不同尾矿区、不同种类的根瘤菌17株,采用重金属平板法测定各供试菌株对贵州尾矿土分布较多的Pb²⁺,Zn²⁺,Cr⁶⁺,Mn²⁺,Cd²⁺五种重金属的耐受性,结果发现17个菌株对3种或3种以上的重金属都具有不同程度的耐受能力,其中HMZT039-6,HSST042-6,HSST042-1,ZHQT052-1和HXTT050-1对5种重金属离子均有耐受能力,为筛选出的最优抗性根瘤菌株。本研究结果可为本地区土壤重金属污染的修复治理提供共生微生物资源。     

土壤重金属特性空间变异性方法研究

环境保护及农业可持续发展成为当今世界人类面临的重要课题,重金属污染尤为突出,并通过食物链进入人体,对人体健康构成极大危害;本文简要分析了土壤重金属的来源,并对土壤重金属特性空间变异定量研究中所涉及的主要分析方法作了初步介绍;提出了重金属特性空间变异定量研究方法的两点启示:①地统计学与3S(GIS,RS,GPS)技术的结合来研究重金属空间变异;②提出了地统计学与土壤重金属空间变异分布建模.     

Characterization of Ca-ATPase activity in gill microsomes of freshwater mussel, Lamellidens marginalis (Lamarck) and heavy metal modulations

Freshwater mussel Lamellidens marginalis showed a Ca²⁺-stimulated ATPase activity in the microsomal fraction of gill. Mg²⁺ was also found to be an activating cation for this ATPase enzyme. Ca²⁺ ATPase showed maximal activity at 40 °C and between pH 7.5–8.0. Substrate specificity of Ca²⁺ ATPase was highest with ATP, followed by GTP and other trinucleotides such as UTP, CTP and ADP also showed some amount of hydrolysis. Ca²⁺ ATPase showed slight inhibition with ruthenium red and sodium vanadate and is insensitive to sodium azide, ouabain, oligomycin B and phenylalanine. Heavy metals like Hg²⁺, Cu²⁺ and Zn²⁺ showed 50% inhibition of Ca²⁺ ATPase activity at concentrations of 0.25 mM, 0.5 mM and 1 mM, respectively whereas Cd²⁺ and Ni²⁺ showed 39% and 28% inhibition of enzyme activity at 1 mM concentrations.     

Use of treated mine water for rainbow trout (Oncorhynchus mykiss) culture: a production scale assessment

In previous proof-of-concept work, it was shown that the use of treated coal mine water for rainbow trout (Oncorhynchus mykiss) culture in a cage was technically feasible, though only a 50-fish bioassay was grown and no work on production-related issues was conducted. To further advance the use of treated mine water, an under-utilized water resource throughout Mid Appalachia, work was conducted to assess the effects of using treated coal mine water for the intensive production of rainbow trout in a flow-through system. During this study, comprehensive water quality data were collected to supplement fish weight and length data taken during routine monthly sampling events. The 8000 fish grew well in the raceway system over the 9 months of production, where a feed conversion ratio of 1.4 and a condition factor of 5.1 × 10⁻⁴ were measured with stocking and harvest densities of 26.4 and 50.2 kg/m³, respectively. Further, total net production was 3275 kg (7220 lb) with 98.6% survival. Throughout the study, dissolved ion concentrations (Fe, Al, Mg, Ca, and SO₄) often exceeded recommended tolerance limits. Further, elevated ammonia nitrogen concentrations generated from a component of the mine water-treatment process were identified as a potential limiting factor for aquaculture development. However, when the non-ideal effects of high ionic strength and the speciation of dissolved metal–ligand complexes were taken into account, the concentrations of free metal ions were within recommended tolerance limits.     

生态环境中重金属的化学形态转化及影响因素研究

提出了环境中重金属的化学形态发生转化的2种途径,探讨了影响此转化过程的环境条件,包括pH、氧化还原电位和吸附质表面的键合作用等。     

Interaction of Cd-hyperaccumulator Solanum nigrum L. and functional endophyte Pseudomonas sp. Lk9 on soil heavy metals uptake

Bioaugmentation is a promising method for assisting phytoextraction of heavy metals from contaminated soil, and the development of bioaugmentation-assisted phytoextraction requires the understanding of the mechanism involved in the interaction between plants and inocula. In this study, a pot study was conducted to evaluate the effect of bacterial endophyte Pseudomonas sp. Lk9 which can produce biosurfactants, siderophores and organic acids on the growth and metal uptake of Cd-hyperaccumulator Solanum nigrum L. growing in multi-metal-contaminated soil. The results revealed that Lk9 inoculation could improve soil Fe and P mineral nutrition supplies, enhance soil heavy metal availability, and affect host-mediated low-molecular-weight organic acids secretion, thereby significantly increasing S. nigrum shoot dry biomass by 14% and the total of Cd by 46.6%, Zn by 16.4% and Cu by 16.0% accumulated in aerial parts, compared to those of non-inoculated control. The assessment of phytoextraction showed that Lk9 inoculation elevated the bioaccumulation factor of Cd (28.9%) and phytoextraction rates of all metals (17.4%, 48.6% and 104.6% for Cd, Zn and Cu, respectively), while the translocation factors had negligible difference between Lk9 inoculation (3.30, 0.50 and 0.40 for Cd, Zn and Cu, respectively) and non-inoculated control (2.95, 0.53 and 0.42 for Cd, Zn and Cu, respectively). It was also found that the symbiotic association between S. nigrum and Lk9 significantly increased the soil microbial biomass C by 39.2% and acid phosphatase activity by 28.6% compared to those in S. nigrum without Lk9. This study would provide a new insight into the bioaugmentation-assisted phytoextraction of heavy metal-contaminated soils.     

Response of ammonia oxidizing microbes to the stresses of arsenic and copper in two acidic alfisols

Soil pollution by elevated heavy metals exhibits adverse effects on soil microorganisms. Ammonia oxidizing bacteria and ammonia oxidizing archaea perform ammonia oxidative processes in acidic soils. However, influence of heavy metal stress on soil ammonia oxidizers distribution and diversity is inadequately addressed. This study investigated the responses of ammonia oxidizing bacteria and archaea to heavy metals, Cu and As during short-term laboratory experiment. Two different acidic alfisols named as Rayka and Hangzhou spiked with different concentrations of As, Cu and As + Cu were incubated for 10 weeks. Significant reduction in copy numbers of archaeal-16S rRNA, bacterial-16S rRNA and functional amoA genes was observed along elevated heavy metal concentrations. Ammonia oxidizing archaea was found to be more abundant than ammonia oxidizing bacteria in all the heavy metal treatments. The potential nitrification rate significantly decreased with increasing As and Cu concentrations in the two soils examined. Denaturing gradient gel electrophoresis analysis revealed no apparent community shift for ammonia oxidizing archaea even at higher concentrations of As and Cu. Phylogenetic analysis of archaeal amoA gene from 4 clone libraries indicated that all the archaeal amoA sequences were placed within 3 distinct clusters from soil and sediment group 1.1b of Thaumarchaeota. Our results could be useful for the better understanding of the ecological effects of heavy metals on the abundance and diversity of soil ammonia oxidizers.     

The effects of applied crop residues on losses of Fe, Mn, Cu and Zn in run-off from a soil prone to crusting

Tillage practices may reduce the organic matter content in near-surface soil horizons causing crust formation. Surface conditions may cause an increase in surface run-off, thus enhancing contaminant transfer of heavy metals or an acceleration in nutrient loss. This study examines the effect of applying crop residues to the surface of tilled soils on heavy metal losses by run-off. Losses in iron (Fe), manganese (Mn), zinc (Zn) and copper (Cu) were analysed. Run-off and sediment yield were measured on 1 m² plots using a rainfall simulator with a constant 65 mm/h intensity. Four successive rainfall applications were performed, the first three at 25 mm each and the last at 65 mm. Added corn straw varied between 0 and 4 t/ha in the five studied treatments. After 140 mm cumulative rainfall, total heavy metal losses were as follows: Fe from 137 to 950 mg/L, Mn from 2.3 to 12.83 mg/L, Cu from 0.09 to 0.72 mg/L and Zn from 0.31 to 2.46 mg/L. Dissolved fractions were as follows: Fe from 0.014 to 0.229 mg/L, Mn from 0.034 to 1.45 mg/L, Cu from 0.002 to 0.013 mg/L and Zn from 0.02 to 0.12 mg/L. Total concentrations of the studied elements decreased exponentially due to the effect of corn straw on soil loss. However, dissolved contents of Fe and Cu scarcely varied. Significant positive linear correlations were observed between total heavy metal content and soil and sediment loss by run-off. It is concluded that the addition of straw to a soil of low fertility prevents heavy metal loss.