热激对温敏核不育系“安农S-1”花药生理变化的影响

试验研究热激对温敏核不育系“安农S-1”花药生理变化的影响结果表明,温敏核不育系“安农S-1”在花粉母细胞形成至减数分裂期经热激(38℃)处理后,其花药中游离氨基酸总量、天冬氨酸和丙氨酸含量均显著高于低温(23℃)处理;而游离脯氨酸和可溶性蛋白质含量则显著低于低温处理。对照经热激(38℃)处理后,其花药中游离氨基酸和可溶性蛋白质含量与低温处理基本一致,这些生理变化可能影响花粉母细胞正常的形成和减数分裂,以致花粉不能正常发育并最终导致花粉败育。     

糙米发芽工艺参数研究

[目的]优化糙米发芽工艺参数.[方法]以黑龙江省糙米为原料制备发芽糙米,通过单因素试验确定最佳的糙米浸泡温度、浸泡时间、发芽温度、发芽时间,紫外分光光度法快速测定确定糙米及发芽糙米中的γ-氨基丁酸和游离氨基酸的含量为指标,进行正交试验.[结果]试验表明,浸泡温度为30℃,浸泡时间为10 h,发芽温度为35 ℃,发芽时间为34 h,此条件下γ-氨基丁酸的积累量达最高.浸泡温度为35℃,浸泡时间为12 h,发芽温度为25 ℃,发芽时间为34 h,此条件游离氨基酸积累量达最高.[结论]研究可为进一步了解黑龙江省七台河糙米产品提供依据.     

我国《反垄断法》立法中几个基本问题

目前，我国反垄断方面还没有形成完整的法律体系，制定一部与已有的《反不正当竞争法》相互协调，对市场竞争起到保护和促进作用的《反垄断法》已是当务之急。在此立法中，要明确反垄断法的立法宗旨、反垄断法规范制约的垄断行为、反垄断法的列外、反垄断执法机构的设置与职责，以及垄断行为应承担的法律责任等几个主要问题。     

植物组织中自由水和束缚水含量测定方法的改进

在对测定植物组织自由水和束缚水的常规方法——阿贝折射仪法的误差来源进行分析的基础上,对其测定方法进行了改进,将测定糖液的浓度改为直接测定叶片的质量,通过测试不同蔬菜的组织材料,确定了改进后的测定方法——称重法。称重法操作简便,误差小。     

Protective effects of diltiazem on liver,pancreas and small intestine in hemorrhagic-shock canine

AIM: To investigate the protective effects of diltiazem (Dil) on liver, pancreas and small intestines in hemorrhagic-shock canines and its mechanism. METHODS: The canines were bled to a mean arterial pressure (MAP) of 5.33-6.67 kPa for 30 min to produce the model of shock. During the shock, the dogs received water-soluble calcium channel blocker Dil or normal saline. The MAP was kept at the level for 90 min, then the total blood which was bled previously was reperfused. They were observed for 240 min. RESULTS: Dil could significantly elevated MAP of the hemorrhagic-shock canines (P<0.01) and the activity of superoxide dismmutase (SOD) of pancreas tissue (P<0.01), also it could decrease content of malondialdehyde (MDA) of the liver, pancreas and small intestine tissues (P<0.01). Electron microscope data indicated that Dil-treated dogs have a normal ultrastructure in the liver, pancreas and small intestine tissues. CONCLUSION: Dil can protect the structure and function of the liver, pancreas and small intestine in hemorrhagic-shock canines.     

仔猪红细胞内游离原卟啉微量测定法及正常值探讨

利用荧光分析法建立了猪红细胞内游离原卟啉（ＦＥＰ）微量测定法。经回收率和重复性测定，证明该法准确度高，完全符合实验要求。可以用于仔猪缺铁性贫血的诊断，并可以作为研究仔猪铁代谢的一个主要参数。应用本法首次测定了３～３０日龄仔猪在补铁与未补铁情况下ＦＥＰ值和ＦＥＰ／Ｈｂ比值。补铁组ＦＥＰ值为１８５．２４±１３．１２～２５８．６３±２１．０９μｇ／ｄｌＲＢＣ；ＦＥＰ／Ｈｂ比值为７．００±０．５４～９．４３±０．７３μｇ／ｇＨｂ，未补铁组ＦＥＰ值为１９４．６９±９．２３～２４７．８８±１５．１４μｇ／ｄｌＲＢＣ；ＦＥＰ／Ｈｂ比值在６．９６±０．３３～１０．１９±０．７３μｇ／ｇＨｂ。关键词     

Effect of mild hypothermia on energy metabolism and hydroxyl radical production after cerebral ischemia/reperfusion in gerbils

AIM: To study the effect of mild hypothermia on energy metabolism and hydroxyl radical production as well as delayed neuronal death (DND) in hippocampus during cerebral ischemia/reperfusion in gerbils.METHODS: Forebrain ischemia was induced by occluding bilateral common carotid arteries with aneurysm clamps for 10 min in gerbils. The DND in hippocampal CA1 sector was assessed by histological examination, and hydroxyl radical, ATP (adenosine triphosphate), ADP (adenosine diphosphate),AMP (adenosine monophosphate) levels were determined by high performance liquid chromatography with electrochemical or ultraviolet detection. RESULTS: The number of survival neuronal in hippocampal CA1 sector in mild hypothermia + I/R group was more than that in I/R group after ischemia/reperfusion 96 h. The content of 2,3-DHBA (2,3- dihydroxybenzoic acid) in hippocampus in I/R group was much higher than those in sham operation and mild hypothermia + I/R group after reperfusion 6 h (P＜0.01), but there were no significant differences in 2,3-DHBA outputs among 3 groups 48 h and 96 h after reperfusion. There were no obvious differences in ATP, ADP, AMP level in hippocampus among 3 groups 6 h after reperfusion. However, the content of ATP,ADP,AMP in mild hypothermia + I/R group was much higher than those in I/R group 48 and 96 h after reperfusion (P＜0.01).CONCLUSION: Mild hypothermia can reduced DND by improving the cerebral energy metabolism during forebrain ischemia/reperfusion in gerbils.     

低温胁迫对割手密和斑茅游离脯氨酸含量的影响(Ⅱ)

研究了来自不同原生地甘蔗近缘野生种割手密、斑茅无性系叶片中游离脯氨酸(Fpro)的含量及其与低温的关系。结果表明,冬季割手密和斑茅叶片中Fpro的含量与其原生地的海拔、纬度之间呈极显著的正相关关系,冬季的Fpro含量平均值分别是夏季的11.4和7.7倍,导致Fpro积累的真正原因是低温;在持续低温胁迫条件下,割手密、斑茅叶片中Fpro含量呈波动式增长,在作变温处理时Fpro的消长是可逆的。叶片中Fpro含量与其抗寒性关系十分密切的特点,表明它可以作为甘蔗近缘野生种抗寒力鉴定的指标。     

不同光周期条件下PhyB和ABA对不同苜蓿品种的秋眠性调控

短日照和低温是导致苜蓿秋眠的主要环境因子。本研究检测了3个不同秋眠型苜蓿在8,12和16 h光照光周期条件下叶片和顶芽中PhyB和ABA的含量,并探讨其对秋眠的影响机制。结果表明,1)和非秋眠苜蓿相比,秋眠苜蓿叶片中PhyB含量高,PhyB作为绿色植物主要光受体可能通过光周期调控或参与了苜蓿的秋眠;并且3个品种均是在8 h光照下PhyB合成量高,暗示长暗期利于其合成。2)和12,16 h光照相比,8 h处理顶芽和第一展开叶中ABA含量最高,显然短日照利于ABA的合成;8 h光照处理时,秋眠型苜蓿品种Vernal ABA含量分别比半秋眠型CW5 0 2和非秋眠型CUF1 0 1高2.1 2和2.0 3倍,1 2 h光照处理ABA含量Vernal比CW5 0 2和CUF1 0 1分别高3 6.2 4%和46.46%,但16 h光照处理ABA含量并不表现上述规律,说明8和12 h光照程度不同地为苜蓿创造了逆境效应,ABA可能是调控或诱导秋眠的激素。3)8 h光照下苜蓿秋眠特征明显,生长严重受抑,不开花;12 h光照下苜蓿秋眠特征比8 h光照处理轻,生长受抑但抑制程度低于8 h光照处理,不开花;16 h光照下3个品种生长、开花正常。可能是PhyB含量多寡程度不同地调控了光周期反应,进而影响了ABA合成,而ABA在不同光周期诱导下通过合成量不同调控了不同秋眠基因表达,诱发了秋眠。     

香木莲对短时低温胁迫处理的生理生态响应

通过测定木兰科植物香木莲在人为短时低温处理下的一些生理生化指标的变化情况,可以分析香木莲苗木的抗性生理情况,以期对香木莲的抗寒性进行评价.实验结果表明,香木莲的叶片在经短时低温处理后,其脯氨酸含量、丙二醛含量及相对电导率均表现为不同程度的上升.而叶片的叶绿素荧光指数的变化则表现为随处理时间的延长,叶片的原初荧光(Fo)上升,其光化学效率(Fv/Fm)及可变荧光/原初荧光比率(Fv/Fo)则表现为随处理时间的延长而降低.这表明,短时低温处理对叶片的叶绿体光系统的潜在活性及量子传递效率造成了一定的影响,结合生理生化指标的变化,可以部分解释香木莲抗寒机制.