NDV融合蛋白裂解位点基因的重组昆虫杆状病毒表达载体的构建与鉴定

将新城疫病毒（ＮＤＶ）Ｆ４６Ｅ９株和ＬａＳｏｔａＥ４株的融合蛋白裂解位点（Ｆｃ）基因从本实验室构建的重组质粒ｐＵＣＦ４６Ｆｃ和ｐＵＣＬａＦｃ中用ＥｃｏＲＩ和ＳａｌⅠ切出。将这２个毒株的Ｆｃ基因定向克隆入昆虫杆状病毒表达载体ｐＳＸＩＶＶＩ＋Ｘ３的ＥｃｏＲＩ和ＳａｌⅠ位点之间，获得２个毒株的Ｆｃ基因克隆ｐＸ３Ｆ４６Ｆｃ和ｐＸ３ＬａＦｃ。重组质粒用ＥｃｏＲＩ／ＳａｌⅠ、ＰｓｔⅠ酶切法、ＰＣＲ和核酸探针法进行了鉴定，证明插入的基因来自ｐＵＣＦ４６Ｆｃ和ｐＵ－ＣＬａＦｃ，插入的位置和方向都正确。这２个载体的构建为Ｆｃ基因在昆虫细胞系统的表达创造了条件。     

哈尔滨北方森林动物园观赏肉食目动物寄生蠕虫感染情况调查

为了解哈尔滨北方森林动物园观赏肉食目动物寄生蠕虫的感染状况,于2009年3～5月采用饱和盐水漂浮法和水洗沉淀法对犬、猫、熊和浣熊4科的67只观赏肉食目动物粪便进行了蠕虫虫卵检查。结果有19只检出虫卵,感染率为28．4％。其中猫科、熊科、浣熊科、犬科的感染率分别为63．6％、16．7％、14．3％和5．0％,在检出的虫卵中,各种蛔虫卵占优势。该调查为动物园制定肉食目动物驱虫方案奠定了基础。     

岷县草产业发展现状及策略

岷县地处秦岭、黄土高原和青藏高原的交汇带。本研究通过分析其草产业现状,指出岷县草产业发展主要存在天然草地退化严重、栽培草地管理粗放和特色草产业加工技术不完善的问题,提出岷县草产业今后发展的方向是分区管理和分类经营,从建立草地生态补偿基金、发展草地观光旅游业、提高栽培草地生产力、提高草地资源的利用效率和培训农民能手等方面提出了岷县草产业的发展策略。     

嘉兴平原桑园土壤分类及肥力的调查

选择浙北嘉兴平原桑园代表性土壤，进行调查，研究其成因、分类及特性，并对高低产桑园土壤的理化性状进行化验分析，明确了嘉兴平原桑园土壤是历史上由人工堆迭而成，在分类系统上归属于潮湿土纲、正常潮湿土亚纲、潮土土类、暗潮土亚类、堆迭土土属，又分为粘质堆迭土、壤质堆迭土、粉质堆迭土３个土种。在统计众多土壤理化性状数据的基础上，暂拟本区高产桑园土壤１６项理化性状，作为本地区桑园地力建设的参考。     

紫花苜蓿多叶型与三叶型品种同工酶的比较研究

采用聚丙烯酰胺凝胶电泳技术,对国外2种多叶型苜蓿及国内外5种三叶型苜蓿幼苗的5种同工酶进行了测定,将酶谱数量化,应用SPSS统计软件求出各品种间的遗传一致度及欧氏遗传距离,并进行了聚类分析.结果表明:1)测定的5种同工酶中过氧化物酶(PER)、酯酶(EST)、淀粉酶(AMY)具有多态性,综合此3种酶酶谱可以鉴定供试的7个品种;2)2个多叶型品种具有一共同特征酶带PER-4,国内品种的过氧化物酶活性明显高于国外品种;3)品种间的遗传一致度为0.826～0.952,欧氏遗传距离为0.500～1.118,多叶型品种间遗传一致度为0.904,欧氏遗传距离为0.500;4)等级聚类分析结果为,以欧氏遗传距离0.400为分界,供试品种分为3类,2个多叶型品种为一类,其余的5种三叶型品种分为另两类.     

Physiological properties and classification of strains of Treponema sp. isolated from pigs in poland

Fifty-one treponemas were isolated from pigs. Twenty-three isolates with typical morphology and growth characteristic were beta hemolytic, enteropathogenic, produced indole and with exception of three strains did not ferment fructose. These strains were classified as typical T. hyodysenteriae and were usually isolated from pigs with symptoms of mucohemorrhagic diarrhoea. The seventeen other isolates were weakly beta hemolytic after 48 h incubation, enteropathogenic, 12 out of 17 produced indole, 10 out 17 fermented fructose. These strains were usually isolated from pigs with symptoms of gray-green diarrhoea and classified as T. hyodysenteriae 2 biotype or intermediate type. They may be compared with Treponema sp. isolated by Taylor et al. Eleven non enteropathogenic strains showed typical characteristic for T. innocens. Gas chromatography analysis of the fatty acids production from glucose, showed that all isolated treponemas produced acetate and butyrate. Typical T. hyodysenteriae produced additionally propionate. Strains of T. hyodysenteriae biotype 2 produced propionate or isobutyrate as well.     

草原综合顺序分类法的新改进：Ⅰ类的划分指标及其分类检索图

根据全国２３５２个气象站３０年以上的气候资料以及其他资料，对草原的综合顺序分类法第一级分类的热量级，湿润度级指标进行了修正，设计了新的分类检索图，将地带性和非地带性的天然草地，以及人工草地的类，纳入到统一的检索图的中，并可进行计算机检索。     

Influence of Land-Use Legacies Following Shrub Reduction and Seeding of Big Sagebrush Sites

Big sagebrush (Artemisia tridentata Nutt.) plant communities often require management to reduce shrub density and rehabilitate understory vegetation. We studied vegetation responses to a two-way chain harrow treatment and broadcast seeding of 12 herbaceous species at eight Wyoming big sagebrush (A. tridentata Nutt. subsp. wyomingensis Beetle & Young) sites. These sites differed in land-use history; five were cultivated for dryland wheat production during the 1950 ? 1980s and then seeded with introduced forage grasses (C-S), while three had not been exposed to this land-use legacy (non C-S). Our objective was to evaluate whether the C-S legacy influences the magnitude of vegetation change following contemporary treatment. Before treatment, C-S sites had lower sagebrush cover, higher dead sagebrush cover, and higher broom snakeweed (Gutierrezia sarothrae [Pursh] Britton & Rusby) cover than adjacent non C-S sites. Plant community change 3 years after treatment, determined with multivariate ordination analysis of species composition, varied between site histories, and response to treatment was most strongly correlated with reductions in sagebrush cover, increases in perennial grasses, and increases in 10 other herbaceous species—including some undesirable species and four that were seeded in 2010. Five years after treatment, mature sagebrush cover remained reduced for both land-use histories, yet density of sagebrush seedlings and broom snakeweed increased in C-S sites during the second and third years after treatment. In addition, perennial forb cover increased for C-S sites, while perennial grass biomass increased for non C-S sites. Our results emphasize that broad variability in plant community responses to sagebrush reduction and seeding is possible within the same ecological site classification and that legacy effects due to the combination of past cultivation and seeding should be considered when planning restoration projects, including the consideration that seeding may not always be necessary on C-S sites.     

小RNA病毒基因组3'非编码区.结构与功能研究进展

小RNA病毒属于单股正链RNA病毒,包括肠道病毒属、鼻病毒属、心脏病毒属、口疮病毒属和肝病毒属等几个主要的属.口蹄疫病毒(FMDV)、脊髓灰质炎病毒(PV)、丙型肝炎病毒(HCV)、脑心肌炎病毒(EMCV)是该科病毒的典型代表.小RNA基因组的末端具有非编码区(UTR),是病毒基因组复制的主要调控位点,3'非编码(3'...