Expression of vascular endothelial growth factor in canine oral malignant melanoma

Serum, plasma and tissue expression of vascular endothelial growth factor (VEGF) was measured in 20 dogs previously diagnosed histologically with oral melanoma. The concentrations of VEGF in serum and plasma were significantly higher in dogs with melanoma than in a control population (P ≤ 0.002). Concentrations of VEGF in the serum and plasma of dogs with melanoma were highly correlated (r = 0.867). Ninety‐five per cent of melanoma tissues expressed VEGF. Two staining patterns were detected: diffuse and granular cytoplasmic staining. High blood concentrations of VEGF were correlated to a shorter survival time in dogs receiving definitive therapy (P = 0.002). Survival times were significantly longer in dogs receiving definitive therapy versus palliative therapy (median 496 versus 97 days, P = 0.007). Blood concentrations of VEGF were associated with stage (P < 0.05). Dogs with oral melanoma have increased serum, plasma and tissue concentrations of VEGF. Increased expression of VEGF may be a reasonable target for future therapy of canine oral melanoma.     

赣南脐橙园蜘蛛优势种的季节动态及与气象因子的相关分析

赣南脐橙园蜘蛛已知13科38种。脐橙树冠层蜘蛛优势种有3种,其中草间钻头蛛(Hylyphantes graminicola)全年以5 - 6月及9 - 10月份的发生数量最大;斑管巢蛛(Clubiona deletrix)在7 - 10月份发生量较大,2 - 3月份的数量明显减少;鳞纹肖蛸(Tetragnatha squamata)在4月和12月份的种群数量较多,而6 - 9月份较少见到。地面层蜘蛛优势种有2种,其中草间钻头蛛的季节消长同样具有两个高峰期,但跨度较少,仅在6月份和10月份出现;拟水狼蛛(Pirata subpiraticus)种群数量在3月份达到最高峰,在12月份处于低谷。不同季节脐橙树冠层草间钻头蛛和斑管巢蛛的发生数量都与平均气温和日照时数呈显著或极显著正相关,而鳞纹肖蛸的季节发生数量与平均气温间为显著负相关;地面层拟水狼蛛的数量季节变动与相对湿度和降雨量间表现出极显著的正相关性。     

Risk factors for outbreaks of infectious salmon anemia in farmed Atlantic salmon, Salmo salar

Infectious salmon anemia (ISA) is a viral disease occurring in farmed Atlantic salmon (Salmo salar) that is characterized by lethargy, anorexia, anemia and death. To control the disease in New Brunswick, Canada, 7.5 million fish from outbreak cages have been destroyed since 1997. Despite changes made by farmers, 2002 was the worst year ever for ISA losses in the region.

We evaluated the associations between potential risk factors and ISA outbreaks in the Atlantic-salmon sites in New Brunswick. This was a multilevel study in which the site-level design was a retrospective cohort study while the cage-level design was a modified case-cohort study. The questionnaire was divided into site-level questions, cage-level questions and hatchery information.

The important factors identified by this study can be categorized as environmental, farmer controlled or industry controlled according to the capacity to change or eliminate them. Environmental risk factors such as increasing the depth of the net (if nets were ≤9 m, odds ratio (OR) = 3.34) and decreasing the depth of water underneath the net (if depth of water underneath the net >3 m, OR = 3.34) are for the most part dictated by site location. Wild pollock (Pollachius virens) in the cage reflects the number of wild pollock that live in the site location. If there were ≥1000 pollock in the cage, the odds of disease in the cage increased 4.43-fold. Risk factors that are under farm control include increasing the number of times that the salmon are treated for sea lice (OR = 3.31 if lice treatments are ≤2 times), transferring small smolts into seawater (OR = 2.40 if smolts weighed >99 g) and improving on the adaptation of smolts to seawater to reduce post-transfer mortalities (OR = 4.52 if there was at least one cage with post-transfer mortalities >5%). The industry-controlled factors need to be addressed by the industry as a whole. Organizing boat travel to minimize the time and frequency of boats travelling to or by sites currently is being reviewed. This will be extremely important because the OR = 9.43 if processing boats travel within 1 km of the site and the OR = 4.03 if the site has dry feed delivered by the feed company. Because the hazard ratio increased stepwise from 1 if the nearest neighbor with ISA was ≥5 km up to 5.5 if the nearest site with ISA was within 0.5 km, increasing the distance between sites might be necessary for effective control.     

松嫩平原西部荒漠化景观动态及驱动力研究-以吉林省大安市为研究区域

以松嫩平原西部荒漠化的典型区域大安市为研究对象,在GIS平台下,根据1986年,1996年和2000年TM影像解译获取的景观动态数据,分析近15年大安市荒漠化土地动态变化规律。并应用Markov模型预测大安市景观格局动态,结果表明如不采取合理有效的措施,2050年荒漠化土地将占土地总面积的42.30%,高-中覆盖度草地,水域,沼泽将大面积退化,分别仅占1986年水平的48.69%,24.71%和22.74%。在此基础之上,运用灰色关联方法分析了土地荒漠化的主要驱动因子。近15年来筑路、开荒等不合理的社会经济活动是土地荒漠化的主要驱动因子;气候因子的变动有利于土地荒漠化过程的发展,是土地荒漠化过程的背景因子;水文因子迭加了人类的无序开发、利用,是土地荒漠化过程的重要影响因子。     

Variability of pesticide residues in crop units

The results of 89 new field trials and 11 supervised trials were considered, together with 91 sets of residue data evaluated earlier. The datasets consisted of 22,643 valid residue data. As all variability factors calculated from individual sample sets are affected by the uncertainties of sampling and analysis, the average of the P(0.975)/R(ave) (97.5th percentile of the residue population divided by the average residues in the lot) values gives the best estimate for the variability factor. The Harrell-Davis (H-D) method gave an average value of 2.89 for the variability factor for all samples, while the average variability factors obtained from samples derived from the new field and supervised trials were 2.8 and 2.7 with the IUPAC and H-D methods respectively. The number of residue values below the LOQ in a sample set significantly affects the observed variability factors. It was found that datasets containing over 20% non-quantifiable residues might not reflect the true variability of the residues. Mixing of treated and non-treated commodities may significantly increase the apparent variability. Consequently, only datasets of known origin and consisting of well-quantifiable residues should be used for estimation of the variability factor. Samples taken from marketed lots may not represent a single lot, and thus they have limited value in estimating the variability factor. The large number of residue data confirms the applicability of the default variability factor of 3 adopted by the FAO/WHO for deterministic estimation of the acute intake of pesticide residues.     

氟吡呋喃酮与8种杀虫剂复配对温室白粉虱的联合毒力

为了筛选出对温室白粉虱高效的农药复配组合,本试验选择9种杀虫单剂对田间采集的温室白粉虱进行了毒力测定,并利用共毒因子和共毒系数对氟吡呋喃酮与8种杀虫剂复配组合的联合毒力进行评价,同时对筛选出的药剂进行田间药效试验。结果表明：杀虫单剂毒力测定中,LC₅₀最低的为鱼藤酮,最高的为螺虫乙酯,48 h LC₅₀分别为0.43μg/mL和78.59μg/mL;毒力大小顺序为鱼藤酮>氟啶虫胺腈>除虫菊素>苦参·印楝素>d-柠檬烯>氟吡呋喃酮>啶虫脒>高效氯氰菊酯>螺虫乙酯。共毒系数大于120的复配组合为氟吡呋喃酮与d-柠檬烯5∶1、氟吡呋喃酮与苦参·印楝素1∶2和1∶5,共毒系数分别为268.31、247.80和241.46,LC₅₀分别为5.76、4.51μg/mL和4.34μg/mL。氟吡呋喃酮与d-柠檬烯5∶1复配增效作用明显,为最佳配比。田间药效试验表明,10 d后,17%氟吡呋喃酮可溶液剂与1%苦参·印楝素乳油、5%d-柠檬烯可溶液剂复配对温室白粉虱的防效达到83.5%～83...     

西部生态脆弱区典型县域农用地质量等别对比研究

以云南陆良、重庆丰都、陕西凤翔、新疆阿勒泰以及内蒙古达拉特为例,基于农用地分等成果,对五县农用地质量等别特征及其差异状况进行了对比研究,在此基础上,探讨了五县国家级自然质量等别、利用等别及经济等别与县域内标准耕作制度、农用地结构、土壤条件及灌排条件的相关关系,查明了农用地质量等别限制因素。结果表明:以上述五县为代表的西部地区农用地等别总体偏低,农用地多为中等地和低等地,高等地分布较少,优等地无分布;五县之间等别差异较明显,农用地质量从优至劣大致顺序为陕西凤翔、云南陆良、重庆丰都、新疆阿勒泰、内蒙古达拉特;五县国家级农用地等别与标准耕作制度、土壤条件以及灌排条件密切相关,应根据不同生态脆弱类型区的主导限制因素,制定相应的农用地质量提升策略。     

生态因子在马铃薯甲虫地理分布中的作用

本文依据已有的研究,通过探讨马铃薯甲虫Ｌｅｐｔｉｎｏｔａｒｓａ ｄｅｃｅｍｌｉｎｅａｔａ地理分布与各种生态因子之间的相互关系,阐述了马铃薯甲虫地理分布主要是由气候和寄主综合作用决定的,热量因子决定了该虫在纬度上的分布,寄主在其南界形成中起一定作用,而滞育是其适应气候和寄主季节性变化的最主要机制。     

植物真菌和卵菌病暴发的起源和传播

传染病暴发在植物、动物和人群中很常见。除了少数已发展为流行病和大流行病外,在很大程度上大多数传染病暴发的原因仍未知,植物真菌和卵菌病暴发尤其如此。所有流行病和大流行病都是从局部暴发开始,然后蔓延到更广泛的地理区域,因此了解其初始暴发的原因对于有效预防和控制植物病害流行病和大流行病至关重要。该文首先描述疾病暴发的定义和检测,随后简要描述导致植物传染病暴发的主要原因,包括寄主植物、病原体及其相关的环境因素,以一种真菌和一种卵菌病原体为例简要概述宿主病原体系统,并强调分子工具在帮助揭示病原体的起源和传播及其暴发及大流行方面的作用。由于人为活动及气候的加速变化,植物病害暴发的可能性越来越大,最后提出应该如何应对其暴发。